ReadiUse™Tyramide(TSA)/ Styramide(PSA)优化的反应缓冲液 货号45090-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。

ReadiUse™Tyramide(TSA)/ Styramide(PSA)优化的反应缓冲液

ReadiUse™Tyramide(TSA)/ Styramide(PSA)优化的反应缓冲液

货号 45090 存储条件 在2-8度冷藏保存
规格 1000 Reactions 价格 1200
Ex (nm) Em (nm)
分子量 溶剂


对于许多免疫组织化学(IHC)应用,传统的酶促扩增程序足以实现足够的抗原检测。但是,有几个因素限制了这些程序的敏感性和实用性。酪酰胺信号放大(TSA)已被证明是一种特别通用且功能强大的酶放大技术,具有更高的测定灵敏度。TSA基于HRP在低浓度过氧化氢存在下将标记的含酪胺底物转化为可与HRP或附近的酪氨酸残基共价结合的氧化的高反应性自由基的能力。Power Styramide™信号放大(PSA™)是对TSA系统的最新升级,具有比相应TSA试剂高10至50倍的改进荧光信号。金畔生物是AAT Bioquest的中国代理商,为您提供最优质的TSA/PSA相关产品。 











将100μLDMSO加入到iFluor™染料标记的Styramide™缀合物(例如#45000)的小瓶中,制成100X Styramide™储备液。

2. H2O2储备溶液

向90μL的ddH 2 O中添加10μL的3%过氧化氢(组分B)。
注意:在使用当天准备新鲜的100X H 2 O 2溶液。




每1 mL PSA工作溶液需要1 mL TSA / PSA缓冲液(组分A),10μLStyramide™储备溶液和10μLH 2 O 2储备溶液。
注意:1 mL足够进行10次测试,基于每个盖玻片或96孔微孔板中每孔所需的100μLStyramide™工作溶液。




A Protocol for Double Fluorescent In Situ Hybridization and Immunohistochemistry for the Study of Embryonic Brain Development in Tribolium castaneum.
Authors: Buescher, Marita and Oberhofer, Georg and Garcia-Perez, Natalia Carolina and Bucher, Gregor
Journal: Methods in molecular biology (Clifton, N.J.) (2020): 219-232

Automation of Multiplexed RNAscope Single-Molecule Fluorescent In Situ Hybridization and Immunohistochemistry for Spatial Tissue Mapping.
Authors: Roberts, Kenny and Bayraktar, Omer Ali
Journal: Methods in molecular biology (Clifton, N.J.) (2020): 229-244

Detection of Cytokine Receptors Using Tyramide Signal Amplification for Immunofluorescence.
Authors: Wang, Herui and Pangilinan, Ryan L and Zhu, Yan
Journal: Methods in molecular biology (Clifton, N.J.) (2020): 89-97

Estimating the ploidy of Gracilariopsis lemaneiformis at both the cellular and genomic level.
Authors: Chen, Haihong and Feng, Xiaoqing and Jiang, Minjie and Xiao, Baoheng and Zhang, Jingyu and Zhang, Wei and Hu, Yiyi and Sui, Zhenghong
Journal: Journal of phycology (2020)

Liver fibrosis and CD206+ macrophage accumulation are suppressed by anti-GM-CSF therapy.
Authors: Tan-Garcia, Alfonso and Lai, Fritz and Sheng Yeong, Joe Poh and Irac, Sergio E and Ng, Pei Y and Msallam, Rasha and Tatt Lim, Jeffrey Chun and Wai, Lu-En and Tham, Christine Y L and Choo, Su P and Lim, Tony and Young, Dan Y and D’Ambrosio, Roberta and Degasperi, Elisabetta and Perbellini, Riccardo and Newell, Evan and Le Bert, Nina and Ginhoux, Florent and Bertoletti, Antonio and Chen, Qingfeng and Dutertre, Charles-Antoine
Journal: JHEP reports : innovation in hepatology (2020): 100062

Procedural Requirements and Recommendations for Multiplex Immunofluorescence Tyramide Signal Amplification Assays to Support Translational Oncology Studies.
Authors: Parra, Edwin Roger and Jiang, Mei and Solis, Luisa and Mino, Barbara and Laberiano, Caddie and Hernandez, Sharia and Gite, Swati and Verma, Anuj and Tetzlaff, Michael and Haymaker, Cara and Tamegnon, Auriole and Rodriguez-Canales, Jaime and Hoyd, Clifford and Bernachez, Chantale and Wistuba, Ignacio
Journal: Cancers (2020)

Sensitive Multiplexed Fluorescent In Situ Hybridization Using Enhanced Tyramide Signal Amplification and Its Combination with Immunofluorescent Protein Visualization in Zebrafish.
Authors: Lauter, Gilbert and Söll, Iris and Hauptmann, Giselbert
Journal: Methods in molecular biology (Clifton, N.J.) (2020): 397-409

Tagging the vanA gene in wastewater microbial communities for cell sorting and taxonomy of vanA carrying cells.
Authors: Gallego, Sara and Barkay, Tamar and Fahrenfeld, N L
Journal: The Science of the total environment (2020): 138865

The autophagic response to Staphylococcus aureus provides an intracellular niche in neutrophils.
Authors: Prajsnar, Tomasz K and Serba, Justyna J and Dekker, Bernice M and Gibson, Josie F and Masud, Samrah and Fleming, Angeleen and Johnston, Simon A and Renshaw, Stephen A and Meijer, Annemarie H
Journal: Autophagy (2020): 1-15

A Hybrid Detection Method Based on Peroxidase-mediated Signal Amplification and Click Chemistry for Highly Sensitive Background-free Immunofluorescent Staining.
Authors: Antonov, Stanislav A and Novosadova, Ekaterina V and Kobylansky, Andrey G and Tarantul, Vyacheslav Z and Grivennikov, Igor A
Journal: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society (2019): 771-782

ReadiUse™Tyramide(TSA)/ Styramide(PSA)优化的反应缓冲液.pdf