代理商T4 DNA Ligase (5 U/µL) 试剂_200units
Thermo Scientific T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5′-phosphate and 3′-hydroxyl termini in duplex DNA or RNA. The enzyme repairs single-strand nicks in duplex DNA, RNA, or DNA/RNA hybrids. It also joins DNA fragments with either cohesive or blunt termini, but has no activity on single-stranded nucleic acids.
T4 DNA Ligase requires ATP as a cofactor.
• Active in Themo Scientific restriction enzyme, PCR, and RT buffers (when supplemented with ATP)
• Cloning of restriction enzyme generated DNA fragments
• T4 DNA Ligase
• Binding of T4 DNA Ligase to DNA may result in a band shift in agarose gels. To avoid this, incubate samples with 6X DNA Loading Dye & SDS Solution at 70°C for 5 min or 65°C for 10 minutes and chill on ice prior to electrophoresis.