Harlan TD.88137 高脂饲料 Adjusted Kcal Diet (42% from fat)

Harlan TD.88137 高脂饲料 Adjusted Kcal Diet (42% from fat)
Harlan Teklad动物饲料
Harlan TD.88137 高脂饲料 Adjusted Kcal Diet (42% from fat)
A staple of atherosclerosis research
More than 25 years ago, our nutritionists collaborated with researchers at Rockefeller University to develop a diet with features of a ‘Western Diet’ to characterize and enhance atherosclerosis development in their newly generated Apoe deficient mouse model. With over 200 unique users worldwide, TD.88137 continues to be fed to genetically modified cardiovascular models to accelerate and enhance hypercholesterolemia and plaque formation.
Formula                                             g/Kg
Casein                                              195.0
DL-Methionine                                     3.0
Sucrose                                            341.46
Corn Starch                                       150.0
Andydrous Milkfat                               210.0
Cholesterol                                          1.5
Cellulose                                              50.0
Mineral Mix, AIN-76 (170915)               35.0
Calcium Carbonate                               4.0
Vitamin Mix, Teklad (40060)                10.0
Ethoxyquin                                          0.04
Critical dietary features of TD.88137 related to atherosclerosis development include:
++Cholesterol (0.2% total cholesterol)
++Total fat (21% by weight; 42% kcal from fat)
++High in saturated fatty acids  (>60% of total fatty acids)
++High sucrose (34% by weight)
Typical fatty acid profile of TD.88137
Typical fatty acid analysis,% of diet1                   Mean                             SD
Total                                                                    20.7                                1.5
Saturated fat                                                       12.8                                0.8
Monounsaturated fat                                             5.6                                0.5
Polyunsaturated fat                                             1.0                                  0.2
Unknown2                                                           1.3                                   0.3
Selected nutrient information1
Key points from the literature
TD.88137 has been used to accelerate atherosclerosis development in Apoe and Ldlr deficient models:
++In Apoe deficient mice, plasma cholesterol triples to >1500 mg/dL within three weeks (1, 2). Foam cell and lesion development occurs within 6-10 weeks
(2-4). Fibrous plaque formation is observed at 15 weeks with the development of fibrous caps after 20 weeks (2).
++Ldlr deficient mice fed for two weeks increase plasma cholesterol to >800 mg/dL and triglyceride to >300 mg/dL (5). After six weeks of feeding, hyperglycemia, hyperinsulinemia and dyslipidemia develop with small foam cell lesions in the aortic
arch (6, 7).
With over 420 citations, uses of TD.88137 continue to evolve and include atherosclerosis, obesity, non-alcoholic steatohepatitis (NASH), osteoporosis,
hypertension and metabolic syndrome. Contact us for a more extensive reference list.
Control diet options for TD.88137
Natural ingredient diets
++Also referred to as standard diets or chow
++Diets differ in the source and level of nutrients as well as the presence of non-nutritive factors (such as phytates or phytoestrogens) compared
to TD.88137
++Limits inferences to differences in dietary pattern versus a specific dietary component
Ingredient matched, low fat diets
++Controls for the type of ingredients, non-nutritive components and the source and level of specific nutrients
++Suggested ingredient matched, low fat dietary controls for TD.88137 listed below; data sheets can be found on our website at envigo.com
Suggested ingredient matched, low fat controls

References  参考文献
1. Plump, A.S., et al., Severe hypercholesterolemia and atherosclerosis in
apolipoprotein E-deficient mice created by homologous recombination in
ES cells. Cell, 1992. 71(2): p. 343-53.
2. Nakashima, Y., et al., Apoe-deficient mice develop lesions of all phases of
atherosclerosis throughout the arterial tree. Arterioscler Thromb, 1994.
14(1): p. 133-40.
3. Febbraio, M., et al., Targeted disruption of the class B scavenger receptor
CD36 protects against atherosclerotic lesion development in mice.
J Clin Invest, 2000. 105(8): p. 1049-56.
4. Nakashima, Y., et al., Upregulation of VCAM-1 and ICAM-1 at atherosclerosis-prone
sites on the endothelium in the Apoe-deficient mouse. Arterioscler Thromb
Vasc Biol, 1998. 18(5): p. 842-51.
5. Towler, D.A., et al., Diet-induced diabetes activates an osteogenic gene regulatory
program in the aortas of low density lipoprotein receptor-deficient mice.
J Biol Chem, 1998. 273(46): p. 30427-34.
6. Tsuchiya, K., et al., FoxOs integrate pleiotropic actions of insulin in vascular
endothelium to protect mice from atherosclerosis. Cell Metab, 2012. 15(3): p. 372-81.
7. Huszar, D., et al., Increased LDL cholesterol and atherosclerosis in LDL receptordeficient
mice with attenuated expression of scavenger receptor B1. Arterioscler
Thromb Vasc Biol, 2000. 20(4): p. 1068-73.
8. Yang, B., et al., Changes of skeletal muscle adiponectin content in diet-induced
insulin resistant rats. Biochem Biophys Res Commun, 2006. 341(1): p. 209-17.
9. Schafer, K., et al., Leptin promotes vascular remodeling and neointimal growth
in mice. Arterioscler Thromb Vasc Biol, 2004. 24(1): p. 112-7.
10. Lijnen, H.R., et al., Nutritionally induced obesity is attenuated in transgenic mice
overexpressing plasminogen activator inhibitor-1. Arterioscler Thromb Vasc
Biol, 2003. 23(1): p. 78-84.
11. Maquoi, E., et al., Modulation of adipose tissue expression of murine matrix
metalloproteinases and their tissue inhibitors with obesity. Diabetes, 2002.
51(4): p. 1093-101.
12. VanSaun MN, et al. 2009. High fat diet induced hepatic steatosis establishes a
permissive microenvironment for colorectal metastases and promotes primary
dysplasia in a murine model. Am J Pathol 175:355-64.
13. Dixon LJ, et al. 2013. Caspase-1 as a central regulator of high fat diet-induced
non-alcoholic steatohepatitis. PLoS One 8:e56100.

Harlan MCD饲料 TD.90262 Methionine/Choline Deficient Diet

Harlan MCD饲料 TD.90262 Methionine/Choline Deficient Diet
品牌:Harlan Teklad
英文名称:Methionine/Choline Deficient Diet
Harlan Teklad动物饲料

Amino acid defined | Envigo

A few amino acid defined formulas are shown below. This type of diet is used when a diet needs to be deficient in one or more amino acids, or adjustments need to be made to specific amino acid levels.
Formula examples:

  • TD.99366      Amino acid diet
  • TD.90262      Methionine and choline deficient diet
  • TD.00434      Folic acid deficient diet
  • TD.99386      No lysine AA diet

Sometimes this type of diet is used to further reduce the background level of certain vitamins that are found in protein sources such as casein or vitamin-free (alcohol extracted) casein. Some folic acid deficient diets utilize this approach.
The amino acid profile for most amino acid defined diets is based on work by Harper and Rogers (J. Nutr. (1965) 87:267-276) and is not representative of the amino acid profile of an intact protein source such as casein. Other amino acid profiles can be specified. When an amino acid(s) is removed or reduced, isonitrogenous adjustment can be made if required.

Harlan MCD饲料 TD.90262 Methionine/Choline Deficient Diet

Harlan定制研究饲料 Diet ingredients

Harlan定制研究饲料 Diet ingredients
Harlan Teklad动物饲料
You may want to prepare your own diet at your research site or facility, and if that is the case, you depend on the ingredients being of the highest quality. Envigo can provide you with many of the same ingredients used to produce our advanced Teklad custom research diets so you can develop your own formula. And as always, our nutritional experts are here to help you make the best decisions for your ingredients and ultimate formulation.
Our ingredients
We offer several ingredients for individual sale so that you can develop your own blends.
“VFT” casein

  • “Vitamin-free” test casein (alcohol-extracted) is best suited for purified test diet formulas where fat or vitamin content needs to be precisely controlled
  • The reduced levels of many vitamins in VFT casein make it the preferred protein source for many vitamin studies. Thus, the nickname “Vitamin-free” was given to the extracted casein many years ago

Vitamin mixes

  • The common vitamin mixes shown below use sucrose or corn starch as a carrier
  • Customized vitamin mixes may use cellulose if a non-nutritive carrier is necessary
  • Vitamin mixes are formulated with a diet inclusion rate in mind. Using more or less than recommended could impair the health of your laboratory animal
  • For those preparing their own diets, vitamin premixes are an efficient way to add vitamins to a diet
  • Vitamin mixes should be stored at 4 degrees celsius or lower upon receipt. Storage at –20 degrees celsius is recommended if the mix will be used beyond 1 month
  • Minimum order is 500 g for both stock and custom vitamin mixes
  • Our available vitamin mixes are commonly used in diets, and are available from stock
  • Vitamin mixes are shipped within a few days of order

Our Formula examples

  • CA.40060     Teklad vitamin mix
  • CA.40077     AIN-76A vitamin mix
  • TD.94047     AIN-93 vitamin mix

Mineral mixes

  • The common mineral mixes shown below as examples use sucrose as a carrier
  • Customized mineral mixes could use cellulose or no carrier if a non-nutritive carrier is necessary
  • Our mineral mixes are formulated with a diet inclusion rate in mind. Using more or less than recommended could impair the health of the animal
  • For those preparing their own diets, mineral premixes are an efficient way to add minerals to a diet
  • Mineral mixes should be stored in a dry cool environment and used within a year of receipt. The minimum order is 500 g for both stock and custom mineral mixes
  • These mineral mixes are commonly used in diets, and are available from stock
  • Our mineral mixes are shipped within a few days of order

Formula examples:

  • CA.170915    AIN-76 mineral mix
  • TD.94046      AIN-93G mineral mix
  • TD.94049      AIN-93M mineral mix

Other mixes (may or may not be available from stock):

  • TD.83171     Vitamin mix without A, D, E, choline
  • TD.81062     Iron deficient mineral mix based on AIN-76A
  • TD.79055     Calcium and phosphorus deficient mineral mixed based on AIN-76
  • TD.98057     Calcium and phosphorus deficient mineral mixed based on AIN-93
  • CA.170760   Rogers-Harper mineral mix


  • “无维生素”测试酪蛋白(酒精提取)最适合于那些需要精确控制脂肪或维生素含量的纯正试验饮食配方。
  • VFT酪蛋白中许多维生素含量的降低使得它成为许多维生素研究的首选蛋白质来源。因此,“无维生素”的绰号是在许多年前给提取出来的酪蛋白起的。


  • 以下常见的维生素混合物使用蔗糖或玉米淀粉作为载体。
  • 定制的维生素混合物可能使用纤维素,如果非营养载体是必要的。
  • 维生素混合物的制定考虑到了饮食中的包容率。多用或少用会损害你的实验动物的健康。
  • 对于那些自己准备饮食的人来说,维生素预混剂是向饮食中添加维生素的一种有效方法。
  • 维生素混合物应储存在4摄氏度或更低的接收。如果使用时间超过1个月,建议使用摄氏-20度。
  • 库存和定制维生素混合物的最低订购量为500克。
  • 我们可用的维生素混合物通常用于饮食,并可从库存中获得。
  • 维生素混合物在订购后几天内发货。


  • CA.40060特克拉德维生素混合物
  • CA.40077AIN-76A维生素混合物
  • TD.94047AIN-93维生素混合物


  • 下面所示的常见矿物混合物是以蔗糖为载体的。
  • 如果需要非营养的载体,定制的矿物混合物可以使用纤维素,也可以不使用载体。
  • 我们的矿物质混合物是根据饮食包容率来制定的。多用或少用会损害动物的健康。
  • 对于那些自己准备饮食的人来说,矿物预混料是向饮食中添加矿物质的有效方法。
  • 矿物混合物应存放在干燥、凉爽的环境中,并在收到后一年内使用。库存和自定义矿物混合物的最低订购量为500克。
  • 这些矿物混合物通常用于饮食,并可从库存中获得。
  • 我们的矿物混合物在订货后几天内装运。


  • CA.170915AIN-76矿物混合物
  • TD.94046AIN-93g矿物混合物
  • TD.94049AIN-9300万矿物混合物


  • TD.83171不含A,D,E,胆碱的维生素混合物
  • TD.81062基于AIN-76A的缺铁矿物配料
  • TD.79055AIN-76混合钙磷缺乏矿物
  • TD.98057AIN-93混合钙磷缺乏矿物
  • CA.170760罗杰斯-哈珀矿物混合物

Harlan teklad品牌饲料分类

Harlan teklad品牌饲料分类
Harlan Teklad动物饲料
Teklad diet, bedding and enrichment + Teklad laboratory animal diets + Custom research diets
Custom diets are developed for a specific purpose and benefit from your input and our expertise
Determining the most appropriate diet for your research model can be challenging, but we are here to help. With more than 20,000 unique formulas in our database attained over 40 years, Envigo nutritionists have vast experience to draw upon. To meet the progressive demands of science and to serve you better, we continually add to our database as we acquire new knowledge.
Common uses for custom diets include:
Control Nutrients
Vitamin or mineral adjusted
Protein or amino acid adjusted
Lipid or fatty acid adjusted
Induce Disease
Atherogenic (cholesterol, fat, cholate)
Diet-induced obesity (40-60% kcal from fat)
High carbohydrate (fructose, sucrose)
NaCl adjusted
Cuprizone demyelination
Dose Animals
Control gene expression – doxycycline or tamoxifen containing diets
Addition of customer-supplied ingredients/compounds
You can select from the wide variety of existing diets, or one designed specifically for your purposes. Either way, we encourage you to contact us for complimentary consultation with our nutritionists.
A small number of representative custom research diet formulas are found on our website. If you are unable to find a product code or formula description cited in a publication, or one used previously by your lab or others, contact us.
Harlan teklad品牌饲料分类

实验动物饲料 Teklad laboratory animal diets
标准天然成分饮食 Standard natural ingredient diets
定制研究饮食 Custom research diets
AIN饮食配方 AIN diet formulas
动脉粥样硬化 Atherogenic
饮食诱导肥胖 Diet induced obesity
脂肪/脂调节 Fat/lipid adjusted
碳水化合物调节 Carbohydrate adjusted
蛋白质调节饮食 Protein adjusted diets
维生素调整饮食 Vitamin adjusted diets
矿物调节饮食 Mineral adjusted diets
盐调节(天然成分) NaCl adjusted (natural ingredient)
氨基酸定义 Amino acid defined
多西环素饮食 Doxycycline diets
他莫昔芬饮食 Tamoxifen diets
客户供应的配料 Customer supplied ingredients
基混合体 Basal mixes
异黄酮调节 Isoflavone adjusted
兔子、猪和其他物种 Rabbit, swine and other species
饮食成分 Diet ingredients
药物饮食 Medicated diets

Harlan肥胖模型高酯饲料 Diet induced obesity

Harlan肥胖模型高酯饲料 Diet induced obesity
Harlan Teklad动物饲料
Diet induced obesity
Purified high fat diets used to induce obesity and obesity-related complications such as diabetes and metabolic syndrome typically have 40-60% of energy derived from fat. The diet tables below summarize relevant diet features for several Teklad custom research diets commonly used in rodent models.
Teklad also creates high-fat diets for other species, including pigs, primates, and dogs. Contact us to discuss the use of these diets or one that better meets your needs.

Commonly-used diet-induced obesity (DIO) Teklad rodent diets with 55-60% of calories from fat
Diet features TD.06414 stocked TD.93075 dough TD.07011 pellet
Kcal/g 5.1 4.8
Fat, % Kcal 60 55
Fat Sources,
% by weight
31% lard
3% soybean oil
27.4% vegetable shortening
1.6% corn oil
Fatty acid profile,
% total fat
37% saturated
47% monounsaturated
16% polyunsaturated
28% saturated, 30% trans
28% monounsaturated (cis)
14% polyunsaturated (cis)
% by weight
12.1 9.6
Notes 60F10S poster data
Compare to D12492
Trans fat
Example modifications TD.08500 coconut oil
TD.09766 milk fat
Ingredient matched,
low fat control diets*
TD.06416 (35% sucrose)
TD.08806 (11% sucrose)
TD.93074 (21% sucrose)
TD.120651 (7% sucrose)
References Mouse

*Control diets can be designed in several ways, depending on what features the researcher wants to modify relative to the high-fat diet. These are just a few examples.

Commonly-used diet-induced obesity (DIO) Teklad rodent diets with 40-45% of calories from fat
Diet features TD.06415 TD.08811 TD.88137 stocked TD.95217
Kcal/g 4.6 4.7 4.5 4.3
Fat, % Kcal 45 45 42 40
Fat sources,
% by weight
19.5% lard
3% soybean oil
21% milk fat
2% soybean oil
21% milk fat 10.6% vegetable shortening
4% milk fat
4% soybean oil
Fatty acid profile,
% total fat
36% saturated
46% monounsaturated
18% polyunsaturated
61% saturated
31% monounsaturated
8% polyunsaturated
62% saturated
27% monounsaturated
5% polyunsaturated
34% saturated, 18% trans
29% monounsaturated (cis)
19% polyunsaturated (cis)
% by weight
22.8 36.8 34.5 15.8
Notes Compare to D12451 45F30S poster data “Western Diet”
Cholesterol added
Trans fat
Example modifications TD.110716 milk fat
TD.10670 no dye
TD.130784 lard
TD.120438 no dye
TD.07201 lard
TD.00573 h-coconut oil, no cholesterol
TD.09682 blue dye
TD.07734 green dye
Ingredient matched,
low fat control diets*
TD.06416 (35% sucrose)
TD.110675 (18% sucrose)
TD.120455 (6% sucrose, resistant starch)
TD.120724 (14% sucrose)
TD.05230 (34% sucrose)
TD.08485 (12% sucrose)
TD.06101 (6% sucrose)
References Mouse



饮食特征 TD.06414 备好 TD.93075 面团 TD.07011 球团
千卡/克 5.1 4.8
脂肪,%千卡 60 55
12.1 9.6
注记 60F10S海报数据
与D 12492比较
示例修改 TD.08500椰子油



饮食特征 TD.06415 TD.08811 TD.88137 备好 TD.95217
千卡/克 4.6 4.7 4.5 4.3
脂肪,%千卡 45 45 42 40
21%乳脂 10.6%蔬菜酥油
22.8 36.8 34.5 15.8
注记 与D 12451比较 45F30S海报数据 “西餐”
示例修改 TD.110716乳脂




Diets for diet-induced obesity (DIO)
Diets with 55-60% of calories from fat like TD.06414 and TD.93075 are commonly used for inducing obesity in rodents. While considered extreme compared to typical human fat consumption, these diets are effective in initiating rapid weight gain in most rodents. With higher fat content there is less room for carbohydrate, thus the carbohydrate (particularly sucrose) amount is relatively low compared to other obesity inducing diets. If you are interested in high fat and high carbohydrate, look at diets with 40-45% of calories from fat (often referred to as western diets).
As the fat level increases, pellet quality (durability) is often compromised. Some higher fat formulas are available only in non-pelleted form or require specific carbohydrate, maltodextrin, for pelleting. Depending on the fat and carbohydrate sources used, the non-pelleted form could be dense and crumbly, dough-like, or paste-like. Though a little more challenging to work with, non-pelleted diet is still used by many researchers for diet-induced obesity models as these researchers suspect the softer form may enhance obesity development.
Diets with 40-45% of calories from fat, like TD.95217, TD.88137, TD.06415, and TD.08811, represent another popular diet pattern for diet-induced obesity work. These diets have double or triple the amount of sucrose found in higher fat diets. High levels of simple carbohydrate like sucrose and fructose may help to promote hypertriglyceridemia, insulin resistance, and fatty liver. Diets with a pattern of high sucrose and high saturated or trans fat are often referred to as “Western Diets” in obesity and cardiovascular fields. Some “Western Diets” have further modifications to the fatty acid profile or even specific vitamin and minerals adjustments to be even more closely matched to a Western Diet pattern. For specific fatty acid modifications, see examples on our fat/lipid adjusted diets page.
Diets for diet-induced diabetes
Many of the same diets used for inducing obesity in rodents can be used to enhance diabetes related phenotypes like insulin resistance and glucose intolerance. However, fasting hyperglycemia characteristic of diabetes (glucose > 200 mg/dL) is uncommon with a diet only approach. Pre-feeding a high fat diet to induce a certain level of obesity and insulin resistance and then giving low-dose streptozotocin (STZ) may be an effective approach if overt hyperglycemia is desired.
Ingredient matched, low-fat DIO control diets
There are many options with different levels and types of fat in addition to different types of carbohydrate ranging from sucrose (highly refined, simple digestion) to corn starch (refined, but more complex) to resistant starch (refined, but not fully digestible). A very basic purified control diet would be AIN-93M (TD.94048) or AIN-93G (TD.94045). AIN-93 diets have a moderate amount of sucrose at ~10%, and fat is from soybean oil with a healthy fatty acid profile. Additional examples of controls for specific DIO diets can be found in the above tables.
Many researchers choose to compare their high fat fed animals to animals fed a natural ingredient, grain-based diet (also referred to as standard diets or chow). These diets differ in the source and level of nutrients as well as in the presence of non-nutritive factors (such as phytates or phytoestrogens). Depending on what your main comparisons are, it may be suitable to have a grain-based diet as your control/reference group. However, making such comparisons limits inferences to dietary patterns versus a specific dietary component.