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IDA 马来酰亚胺 货号12631-AAT Bioquest荧光染料

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IDA 马来酰亚胺

IDA 马来酰亚胺

IDA 马来酰亚胺 货号12631 货号 12631 存储条件 在零下15度以下保存, 避免光照
规格 5 mg 价格 2388
Ex (nm) Em (nm)
分子量 371.35 溶剂 DMSO
产品详细介绍

简要概述

产品基本信息

货号：12631

产品名称：IDA 马来酰亚胺

规格：5mg

储存条件：-15℃避光防潮

保质期：12个月

 

产品物理化学光谱特性

分子量：371.35

外观：固体

溶剂：DMSO

 

产品介绍

固定化金属亲和层析 (IMAC) 是一种常用的蛋白质纯化方法，特别是对于融合到多组氨酸标签的重组蛋白质。通过螯合配体固定在基质上的过渡金属离子与多组氨酸标签相互作用，有效地从样品中隔离融合蛋白。次氮基三乙酸 (NTA) 和亚氨基二乙酸 (IDA) 是市售树脂中常用的两种此类配体。 AAT Bioquest 提供各种 NTA 构建模块，用于开发基于 NTA 的纯化和检测。 IDA 对 NTA 是免费的。三齿 IDA 配体需要比四齿 NTA 更低的咪唑浓度来洗脱蛋白质。 IDA 是一种较小的分子，可以以更高的密度与基质偶联，从而导致更高的金属负载能力。 IDA 马来酰亚胺是一种出色的构建模块，可用于开发各种 IDA 探针，用于检测含多组氨酸的蛋白质，以及用于纯化多组氨酸标记蛋白质的工具。金畔生物是AAT Bioquest的中国代理商，为您提供最优质的IDA 马来酰亚胺。 

 

参考文献

Heterologous overexpression of active hexokinases from microsporidia Nosema bombycis and Nosema ceranae confirms their ability to phosphorylate host glucose.
Authors: Dolgikh, Viacheslav V and Tsarev, Alexander A and Timofeev, Sergey A and Zhuravlyov, Vladimir S
Journal: Parasitology research (2019): 1511-1518

Systematic analysis of the expression, solubility and purification of a passenger protein in fusion with different tags.
Authors: Bernier, Sarah C and Cantin, Line and Salesse, Christian
Journal: Protein expression and purification (2018): 92-106

Production of a truncated recombinant HA1 for influenza A H9 subtype screening.
Authors: Tombari, Wafa and Ghram, Abdeljelil
Journal: Biologicals : journal of the International Association of Biological Standardization (2016): 546-555

Characterization of the Eimeria maxima sporozoite surface protein IMP1.
Authors: Jenkins, M C and Fetterer, R and Miska, K and Tuo, W and Kwok, O and Dubey, J P
Journal: Veterinary parasitology (2015): 146-52

Effects of cholesterol incorporation on the physicochemical, colloidal, and biological characteristics of pH-sensitive AB₂ miktoarm polymer-based polymersomes.
Authors: Yin, Haiqing and Kang, Han Chang and Huh, Kang Moo and Bae, You Han
Journal: Colloids and surfaces. B, Biointerfaces (2014): 128-37

Construction of the coding sequence of the transcription variant 2 of the human Renalase gene and its expression in the prokaryotic system.
Authors: Fedchenko, Valerii I and Kaloshin, Alexei A and Mezhevikina, Lyudmila M and Buneeva, Olga A and Medvedev, Alexei E
Journal: International journal of molecular sciences (2013): 12764-79

Layer-by-layer polyelectrolyte deposition: a mechanism for forming biocomposite materials.
Authors: Tan, Yerpeng and Yildiz, Umit Hakan and Wei, Wei and Waite, J Herbert and Miserez, Ali
Journal: Biomacromolecules (2013): 1715-26

Controlled delivery of recombinant adeno-associated virus serotype 2 using pH-sensitive poly(ethylene glycol)-poly-L-histidine hydrogels.
Authors: Zeng, Yi-Fang and Tseng, S-Ja and Kempson, Ivan M and Peng, Shu-Fen and Wu, Wen-Teng and Liu, Je-Ruei
Journal: Biomaterials (2012): 9239-45

Detection of biological macromolecules on a biochip dedicated to UV specific absorption.
Authors: Robin, Kristelle and Reverchon, Jean-Luc and Mugherli, Laurent and Fromant, Michel and Plateau, Pierre and Benisty, Henri
Journal: Biosensors & bioelectronics (2009): 1585-91

[Isolation, prokaryotic expression and activity analysis of thymidylate kinase (tmk) gene from Phytoplasma of wheat blue dwarf].
Authors: Li, Bei and Ji, Lingling and Wu, Yunfeng and Hao, Xing’an
Journal: Wei sheng wu xue bao = Acta microbiologica Sinica (2008): 739-44

说明书
IDA 马来酰亚胺.pdf

Cy3醛 货号9010-AAT Bioquest荧光染料

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上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品，欢迎访问AAT Bioquest荧光染料官网了解更多信息。

Cy3醛

Cy3醛

Cy3醛 货号9010 货号 9010 存储条件 在零下15度以下保存, 避免光照
规格 5 mg 价格 3732
Ex (nm) 555 Em (nm) 569
分子量 813.90 溶剂 DMSO
产品详细介绍

简要概述

产品基本信息

货号：9010

产品名称：Cy3醛

规格：5 mg

储存条件：-15℃避光防潮

保质期：24个月

 

产品物理化学光谱特性

分子量：813.90

溶剂：DMSO

激发波长(nm)：555

发射波长(nm)：569

消光系数（cm-1 M-1）:150000

荧光量子产率：0.04

校正因子 (280 nm)：0.073

 

产品介绍

Cy3醛是一种可与胺，肼或羟胺反应的活性荧光染料。醛基可与pH 5-9的胺，酰肼或羟胺基反应。与胺反应性琥珀酰亚胺基酯基（NHS）不同，醛可以在酸性pH下与N端胺基反应，这是某些生物缀合反应有时需要的条件。醛与胺基反应形成席夫碱。用氢化物进一步还原将形成稳定的C-N键。醛与其他基团之间的反应可实现荧光素染料的位点特异性缀合和标记，使其靶向分子上的所需位置。可以通过普通的荧光仪器在TRITC或Cy3通道下轻松检测缀合Cy3染料。

点击查看光谱

 

参考文献

Resonance Energy Transfer-Based Nucleic Acid Hybridization Assays on Paper-Based Platforms Using Emissive Nanoparticles as Donors.
Authors: Doughan, Samer and Noor, M Omair and Han, Yi and Krull, Ulrich J
Journal: Methods in molecular biology (Clifton, N.J.) (2017): 301-326

Development of a DNA microarray-based multiplex assay of avian influenza virus subtypes H5, H7, H9, N1, and N2.
Authors: Shi, L and Sun, J-S and Yang, Z-P and Bao, H-M and Jiang, Y-P and Xiong, Y-Z and Cao, D and Yu, X-W and Chen, H-L and Zheng, S-M and Wang, X-R
Journal: Acta virologica (2014): 14-9

Development and application of antibody microarray for lymphocystis disease virus detection in fish.
Authors: Sheng, Xiuzhen and Xu, Xiaoli and Zhan, Wenbin
Journal: Journal of virological methods (2013): 243-9

The role of Src protein in the process formation of PC12 cells induced by the proteasome inhibitor MG-132.
Authors: Tarjányi, Oktávia and Berta, Gergely and Harci, Alexandra and Bacsa, Eszter B and Stark, Borbála and Pap, Marianna and Szeberényi, József and Sétáló, György
Journal: Neurochemistry international (2013): 413-22

A multiplexed screening method for agonists and antagonists of the estrogen receptor protein.
Authors: Li, Zhonghui and Yan, Ming and Li, Zhoumin and Vuki, Maika and Wu, Dan and Liu, Fei and Zhong, Wenying and Zhang, Luyong and Xu, Danke
Journal: Analytical and bioanalytical chemistry (2012): 1373-84

Fluorescence enhancement of silver nanoparticle hybrid probes and ultrasensitive detection of IgE.
Authors: Li, Hui and Qiang, Weibing and Vuki, Maika and Xu, Danke and Chen, Hong-Yuan
Journal: Analytical chemistry (2011): 8945-52

A novel in situ polymerase chain reaction hybridisation assay for the direct detection of bovine herpesvirus type 5 in formalin-fixed, paraffin-embedded tissues.
Authors: Cardoso, Tereza C and Gomes, Deriane E and Ferrari, Heitor F and Silva-Frade, Camila and Rosa, Ana C G and Andrade, Alexandre L and Luvizotto, Maria Cecília R
Journal: Journal of virological methods (2010): 509-12

Identification of S-nitrosated mitochondrial proteins by S-nitrosothiol difference in gel electrophoresis (SNO-DIGE): implications for the regulation of mitochondrial function by reversible S-nitrosation.
Authors: Chouchani, Edward T and Hurd, Thomas R and Nadtochiy, Sergiy M and Brookes, Paul S and Fearnley, Ian M and Lilley, Kathryn S and Smith, Robin A J and Murphy, Michael P
Journal: The Biochemical journal (2010): 49-59

Protein thiol oxidation in murine airway epithelial cells in response to naphthalene or diethyl maleate.
Authors: Spiess, Page C and Morin, Dexter and Williams, Chase R and Buckpitt, Alan R
Journal: American journal of respiratory cell and molecular biology (2010): 316-25

Micropatterning of biomolecules on glass surfaces modified with various functional groups using photoactivatable biotin.
Authors: Choi, Hyun Ju and Kim, Nam Hyun and Chung, Bong Hyun and Seong, Gi Hun
Journal: Analytical biochemistry (2005): 60-6

说明书
Cy3醛.pdf

2-Aminoethoxypropargyl ddGTP 货号17086-AAT Bioquest荧光染料

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上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品，欢迎访问AAT Bioquest荧光染料官网了解更多信息。

2-Aminoethoxypropargyl ddGTP

2-Aminoethoxypropargyl ddGTP

2-Aminoethoxypropargyl ddGTP 货号17086 货号 17086 存储条件 在零下15度以下保存, 避免光照
规格 1 umoles 价格 18288
Ex (nm) Em (nm)
分子量 675.24 溶剂 DMF
产品详细介绍

简要概述

产品基本信息

货号：17086

产品名称：2-Aminoethoxypropargyl ddGTP

规格：1 umoles

储存条件：-15℃避光防潮

保质期：24个月

 

产品物理化学光谱特性

分子量：675.24

外观：液体

溶剂：DMF

激发波长(nm)：N/A

发射波长(nm)：N/A

 

产品介绍

Sanger法是DNA测序中最早最靠谱的方法之一，DNA由四种脱氧核苷酸三磷酸（dNTP）合成。将每个新核苷酸添加到最后的dNTP的3′-OH基团中。可以将二脱氧胸苷三磷酸（ddTTPs）添加到正在生长的DNA链中，但是当它出现时，链延长会停止，因为下一个要连接的核苷酸没有3′-OH。待测序的DNA被制备成单链。该模板DNA带有大量dATP，dGTP，dCTP和dTTP的混合物。加入四种双脱氧核苷酸（ddATP，ddGTP，ddCTP和ddTTP）的混合物，每种混合物均以限量存在，并分别用发不同颜色的荧光的“标签​​”标记。因为所有四个正常核苷酸都存在，所以链延伸正常进行，直到偶然地DNA聚合酶插入ddNTP（而不是正常dNTP）。如果正常核苷酸与双脱氧形式的比率足够高，则在插入ddNTP之前，某些DNA链将成功添加数百个核苷酸，从而终止该过程。在孵育期结束时，片段的长度从最长到最短分离，一个核苷酸之间的差异足以使该链与下一较短和下一较长链分开。当被激光束照射时，四个DDNTP中的每一个都会发出不同的荧光，可以通过自动扫描仪打印输出序列。这些ddATP，ddGTP，ddCTP和ddTTP胺衍生物是开发Sanger测序试剂的重要组成部分。金畔生物是AAT Bioquest的中国代理商，为您提供最优质的2-Aminoethoxypropargyl ddGTP。 

 

参考文献

Polyadenylated sequencing primers enable complete readability of PCR amplicons analyzed by dideoxynucleotide sequencing
Authors: Beranek, M., Drastikova, M., Petera, J.
Journal: Acta Medica (Hradec Kralove) (2012): 160-4

UV-induced bond modifications in thymine and thymine dideoxynucleotide: structural elucidation of isomers by differential mobility mass spectrometry
Authors: St-Jacques, A., Anichina, J., Schneider, B. B., Covey, T. R., Bohme, D. K.
Journal: Anal Chem (2010): 6163-7

Analysis of processivity of mungbean dideoxynucleotide-sensitive DNA polymerase and detection of the activity and expression of the enzyme in the meristematic and meiotic tissues and following DNA damaging agent
Authors: Roy, S., Choudhury, S. R., Sengupta, D. N.
Journal: Arch Biochem Biophys (2008): 55-65

A dideoxynucleotide-sensitive DNA polymerase activity characterized from endoreduplicating cells of mungbean (Vigna radiata L.) during ontogeny of cotyledons
Authors: Roy, S., Sarkar, S. N., Singh, S. K., Sengupta, D. N.
Journal: FEBS J (2007): 2005-23

Mechanism-based suppression of dideoxynucleotide resistance by K65R human immunodeficiency virus reverse transcriptase using an alpha-boranophosphate nucleoside analogue
Authors: Selmi, B., Boretto, J., Sarfati, S. R., Guerreiro, C., Canard, B.
Journal: J Biol Chem (2001): 48466-72

Synthesis of the first ferrocene-labeled dideoxynucleotide and its use for 3′-redox end-labeling of 5′-modified single-stranded oligonucleotides
Authors: Anne, A., Blanc, B., Moiroux, J.
Journal: Bioconjug Chem (2001): 396-405

Improving dideoxynucleotide-triphosphate utilisation by the hyper-thermophilic DNA polymerase from the archaeon Pyrococcus furiosus
Authors: Evans, S. J., Fogg, M. J., Mamone, A., Davis, M., Pearl, L. H., Connolly, B. A.
Journal: Nucleic Acids Res (2000): 1059-66

Structure-based design of Taq DNA polymerases with improved properties of dideoxynucleotide incorporation
Authors: Li, Y., Mitaxov, V., Waksman, G.
Journal: Proc Natl Acad Sci U S A (1999): 9491-6

Characterization of the native and recombinant catalytic subunit of human DNA polymerase gamma: identification of residues critical for exonuclease activity and dideoxynucleotide sensitivity
Authors: Longley, M. J., Ropp, P. A., Lim, S. E., Copel and W. C.
Journal: Biochemistry (1998): 10529-39

Comparative performance of high-density oligonucleotide sequencing and dideoxynucleotide sequencing of HIV type 1 pol from clinical samples
Authors: Gunthard, H. F., Wong, J. K., Ignacio, C. C., Havlir, D. V., Richman, D. D.
Journal: AIDS Res Hum Retroviruses (1998): 869-76

说明书
2-Aminoethoxypropargyl ddGTP.pdf

AF680 NHS 酯 货号1835-AAT Bioquest荧光染料

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上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品，欢迎访问AAT Bioquest荧光染料官网了解更多信息。

AF680 NHS 酯

AF680 NHS 酯

货号 1835 存储条件 在零下15度以下保存, 避免光照
规格 1 mg 价格 2604
Ex (nm) 681 Em (nm) 704
分子量 ~1200 溶剂 DMSO
产品详细介绍

简要概述

产品基本信息

货号：1835

产品名称：AF680 NHS 酯

规格：1mg

储存条件：保存在冰箱-15℃干燥

保质期：12个月

 

产品物理化学光谱特性

分子量：〜1200

外观：固体

溶剂：DMSO

激发波长（纳米）：684

发射波长（纳米）：707

 

产品介绍

AF680 NHS酯（琥珀酰亚胺酯）与AlexaFluor®680NHS酯相同（AlexaFluor®是ThermoFisher的商标）。它是一种鲜红色的荧光染料。AF680染料是水溶性的并且pH从pH4到pH10不敏感。AF680的NHS酯（或琥珀酰亚胺酯）是最方便的胺反应形式，用于将该染料与蛋白质或抗体结合。

点击查看光谱

点击查看实验方案

 

参考文献

A combined solvatochromic shift and TDDFT study probing solute-solvent interactions of blue fluorescent Alexa Fluor 350 dye: Evaluation of ground and excited state dipole moments
Authors: M. K. Patil
Journal: Spectrochim Acta A Mol Biomol Spectrosc (2019): 142-152

Photobleaching Comparison of R-Phycoerythrin-Streptavidin and Streptavidin-Alexa Fluor 568 in a Breast Cancer Cell Line
Authors: S. N. Ostad
Journal: Monoclon Antib Immunodiagn Immunother (2019): 25-29

Comparison between photostability of Alexa Fluor 448 and Alexa Fluor 647 with conventional dyes FITC and APC by flow cytometry
Authors: S. Rai
Journal: Int J Lab Hematol (2018): e52-e54

Development of new hCaM-Alexa Fluor((R)) biosensors for a wide range of ligands
Authors: I. Velazquez-Lopez
Journal: Anal Biochem (2017): 13-22

[Neuroanatomical basis of clinical joint application of “Jinggu” (BL 64, a source-acupoint) and “Dazhong” (KI 4, a Luo-acupoint) in the rat: a double-labeling study of cholera toxin subunit B conjugated with Alexa Fluor 488 and 594]
Authors: J. J. Cui
Journal: Zhen Ci Yan Jiu (2011): 262-7

[Neuroanatomical characteristics of acupoint “Chengshan” (BL 57) in the rat: a cholera toxin subunit B conjugated with Alexa Fluor 488 method study]
Authors: X. L. Zhu
Journal: Zhen Ci Yan Jiu (2010): 433-7

 

相关产品

产品名称 货号
iFluor 594琥珀酰亚胺酯 Cat#1029

说明书
AF680 NHS 酯.pdf

6-NED叠氮化物 货号217-AAT Bioquest荧光染料

发表于由

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品，欢迎访问AAT Bioquest荧光染料官网了解更多信息。

6-NED叠氮化物

6-NED叠氮化物

6-NED叠氮化物 货号217 货号 217 存储条件 在零下15度以下保存, 避免光照
规格 1 mg 价格 3732
Ex (nm) 545 Em (nm) 567
分子量 615.78 溶剂 DMSO
产品详细介绍

简要概述

产品基本信息

货号：217

产品名称：6-NED叠氮化物

规格：1 mg

储存条件：-15℃避光防潮

保质期：12个月

 

产品物理化学光谱特性

分子量：615.78

外观：固体

溶剂：DMSO

 

产品介绍

5′-标记的引物对包括荧光标记的寡核苷酸，在每对中都带有在5’末端选择的染料，以及未标记的寡核苷酸。这些产品可用于多种分子生物学应用。6-NED是用于制备多色荧光寡聚体的一种颜色，该寡聚体是用于基因组片段分析的6-FAM，6-TET，6-VIC，6-HEX，6-NED和6-PET基因表达研究，基因分型和PCR应用。6 -NED叠氮化物是一种非常稳定的荧光探针，可轻松用于标记炔烃修饰的寡核苷酸（包括DBCO修饰的寡核苷酸）。

 

参考文献

Assessment of genetic diversity and bottleneck in Purnathadi buffaloes using short tandem repeat markers.
Authors: Ali, S Sajid and Kuralkar, S V and Das, Ramendra and Raina, Varinder and Kataria, R S and Vohra, Vikas
Journal: Animal biotechnology (2020): 1-12

HLA Class III: A susceptibility region to systemic lupus erythematosus in Tunisian population.
Authors: Hachicha, Hend and Mahfoudh, Nadia and Fourati, Hajer and Elloumi, Nesrine and Marzouk, Sameh and Feki, Sawsan and Fakhfakh, Raouia and Frikha, Faten and Ayadi, Abir and Maatoug, Amira and Gaddour, Lilia and Hakim, Feiza and Bahloul, Zouheir and Makni, Hafedh and Masmoudi, Hatem and Kammoun, Arwa
Journal: PloS one (2018): e0198549

Multicolor-based discrimination of 21 short tandem repeats and amelogenin using four fluorescent universal primers.
Authors: Asari, Masaru and Okuda, Katsuhiro and Hoshina, Chisato and Omura, Tomohiro and Tasaki, Yoshikazu and Shiono, Hiroshi and Matsubara, Kazuo and Shimizu, Keiko
Journal: Analytical biochemistry (2016): 16-22

Multiplex-Ready Technology for mid-throughput genotyping of molecular markers.
Authors: Bonneau, Julien and Hayden, Matthew
Journal: Methods in molecular biology (Clifton, N.J.) (2014): 47-57

Efficient multiplex simple sequence repeat genotyping of the oomycete plant pathogen Phytophthora infestans.
Authors: Li, Ying and Cooke, David E L and Jacobsen, Evert and van der Lee, Theo
Journal: Journal of microbiological methods (2013): 316-22

[Genetic polymorphisms of 15 X-STR loci in Shandong Han population].
Authors: Ma, Teng and Xu, Jing and Yang, Ya-Jun and Sun, Kuan and Xue, Fu-Zhong and Jin, Li and Li, Shi-Lin
Journal: Fa yi xue za zhi (2013): 202-5, 208

A multiplex RTi-PCR reaction for simultaneous detection of Escherichia coli O157:H7, Salmonella spp. and Staphylococcus aureus on fresh, minimally processed vegetables.
Authors: Elizaquível, Patricia and Aznar, Rosa
Journal: Food microbiology (2008): 705-13

Clinical validation of a new triplex real-time polymerase chain reaction assay for the detection and discrimination of Herpes simplex virus types 1 and 2.
Authors: Reil, Heide and Bartlime, Ariane and Drerup, Jana and Grewing, Thomas and Korn, Klaus
Journal: The Journal of molecular diagnostics : JMD (2008): 361-7

Internally controlled triplex quantitative PCR assay for human polyomaviruses JC and BK.
Authors: Dumonceaux, Timothy J and Mesa, Christine and Severini, Alberto
Journal: Journal of clinical microbiology (2008): 2829-36

Developmental validation of a multiplex qPCR assay for assessing the quantity and quality of nuclear DNA in forensic samples.
Authors: Swango, Katie L and Hudlow, William R and Timken, Mark D and Buoncristiani, Martin R
Journal: Forensic science international (2007): 35-45

说明书
6-NED叠氮化物.pdf