钙离子荧光探针Mag-Fluo-4 AM 货号20401-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。

钙离子荧光探针Mag-Fluo-4 AM

钙离子荧光探针Mag-Fluo-4 AM

钙离子荧光探针Mag-Fluo-4 AM    货号20401 货号 20401 存储条件 在零下15度以下保存, 避免光照
规格 10×50 ug 价格 1944
Ex (nm) 495 Em (nm) 528
分子量 817.65 溶剂 DMSO
产品详细介绍

简要概述

关键参数

仪器:荧光酶标仪
Ex:490
Em:525
Cutoff:515
推荐纯黑色孔板

 

仪器:荧光显微镜
通道:FITC
推荐黑色透明底孔板

 

仪器:流式细胞仪
通道:FITC

 

钙离子荧光探针Mag-Fluo-4 AM是美国AAT Bioquest生产的钙离子荧光探针,细胞渗透性Mag-Fluo-4 AM是Fluo-4 AM的类似物,Mg离子的Kd为4.7 mM,Ca离子的Kd为22μM,因此可用作细胞内Mg离子指示剂以及 低亲和力Ca离子指示剂。 这种低亲和力荧光Ca离子指示剂已被用于准确跟踪骨骼肌中空间平均游离Ca离子瞬态的动力学。 Mag-fluo-4产生关于骨骼肌中空间平均游离Ca离子瞬态的可靠动力学信息。金畔生物是AAT Bioquest的中国代理商,为您提供最优质钙离子荧光探针Mag-Fluo-4 AM。 

点击查看光谱

钙离子荧光探针Mag-Fluo-4 AM加载到活细胞中的方案(点击查看)

钙离子篇:时间轴式讲解应用于钙离子检测的探针

产品说明书

操作步骤

1.准备HHBS缓冲液

2.在高质量无水DMSO中制备2 mM至5 mM Mag-Fluo-4 AM储备液
2.1使用的Mag-Fluo-4 AM量:1毫克
2.2所需浓度:2 mM
2.3在合适的容器中,将1mg Mag-Fluo-4AM与611.51μL无水DMSO混合。

 

3.使用10μMMag-Fluo-4 AM 2在HHBS中制备2X工作溶液
3.1最终孔内浓度的Mag-Fluo-4 AM:5μM
3.2在合适的容器中混合16μL的Mag-Fluo-4AM。接下来,添加HHBS或您选择的缓冲液,直到体积为3.2 mL
注意:对于大多数细胞系,我们建议Mag-Fluo-4 AM的最终浓度为4至5μM

4.将100μL染料工作溶液加入已经含有100μL培养基的所需孔中。
4.1该步骤将染料工作溶液从2X稀释至1X,并将每种组分的最终浓度调节至5μM的Mag-Fluo-4 AM

 

5.孵育染料
5.1将染料加载板在细胞培养箱中孵育30-60分钟。
5.2将染料加载板在室温下孵育30分钟。
 

6.准备HHBS缓冲液(或您选择的缓冲液)。
6.1在合适的容器中加入HHBS或您选择的缓冲液,直至体积为4 mL
 

7.用HHBS缓冲液或您选择的缓冲液替换染料工作溶液,使用1.0 mM Probenecid。
7.1首先,从所需孔中除去200μL染料工作溶液和培养基。
7.2在相同的孔中加入200μL含有1.0mM丙磺舒的HHBS(或您选择的缓冲液)。

 

8.运行实验
8.1为您的样品添加所需的处理。
8.2以Ex / Em = 494/516 nm运行实验。

 

参考文献

Real-time intra-store confocal Ca2+ imaging in isolated mouse cardiomyocytes
Authors: Fernandez-Tenorio M, Niggli E.
Journal: Cell Calcium. (2016)

Redistribution of subcellular calcium and its effect on apoptosis in primary cultures of rat proximal tubular cells exposed to lead
Authors: Wang H, Wang ZK, Jiao P, Zhou XP, Yang DB, Wang ZY, Wang L.
Journal: Toxicology (2015): 137

Role of Mitofusin-2 in mitochondrial localization and calcium uptake in skeletal muscle
Authors: Ainbinder A, Boncompagni S, Protasi F, Dirksen RT.
Journal: Cell Calcium (2015): 14

EGF stimulates Mg(2+) influx in mammary epithelial cells
Authors: Trapani V, Arduini D, Luongo F, Wolf FI.
Journal: Biochem Biophys Res Commun (2014): 572

Tetanic Ca2+ transient differences between slow- and fast-twitch mouse skeletal muscle fibres: a comprehensive experimental approach
Authors: Calderon JC, Bolanos P, Caputo C.
Journal: J Muscle Res Cell Motil (2014): 279

TRPC1 is involved in Ca(2)(+) influx and cytotoxicity following Pb(2)(+) exposure in human embryonic kidney cells
Authors: Zhang H, Li W, Xue Y, Zou F.
Journal: Toxicol Lett (2014): 52

High-throughput functional assays of IP3-evoked Ca2+ release
Authors: Tovey SC, Taylor CW.
Journal: Cold Spring Harb Protoc (2013): 930

Measurement and simulation of myoplasmic calcium transients in mouse slow-twitch muscle fibres
Authors: Hollingworth S, Kim MM, Baylor SM.
Journal: J Physiol (2012): 575

Presence of store-operated Ca2+ entry in C57BL/6J mouse ventricular myocytes and its suppression by sevoflurane
Authors: Kojima A, Kitagawa H, Omatsu-Kanbe M, Matsuura H, Nosaka S.
Journal: Br J Anaesth (2012): 352

Sevoflurane protects ventricular myocytes from Ca2+ paradox-mediated Ca2+ overload by blocking the activation of transient receptor potential canonical channels
Authors: Kojima A, Kitagawa H, Omatsu-Kanbe M, Matsuura H, Nosaka S.
Journal: Anesthesiology (2011): 509

说明书
钙离子荧光探针Mag-Fluo-4 AM.pdf

钙离子荧光探针Mag-Fura-2 四钾盐 CAS 132319-57-4 货号20380-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。

钙离子荧光探针Mag-Fura-2 四钾盐 CAS 132319-57-4

钙离子荧光探针Mag-Fura-2 四钾盐 CAS 132319-57-4

钙离子荧光探针Mag-Fura-2 四钾盐  CAS 132319-57-4    货号20380 货号 20380 存储条件 在零下15度以下保存, 避免光照
规格 1 mg 价格 2604
Ex (nm) 336 Em (nm) 505
分子量 586.68 溶剂 Water
产品详细介绍

简要概述

产品基本信息

货号:20380

产品名称:Mag-Fura-2四钾盐*细胞不渗透*

CAS:132319-57-4

规格:1mg

储存条件:-15℃避光防潮

保质期:12个月

化学和光谱特性
外观:固体
分子量:586.68
可溶于:水
激发波长:336
发射波长:503

 

产品介绍

钙离子荧光探针Mag-Fura-2 四钾盐 是美国AAT Bioquest生产的钙离子荧光探针,Mag-Fura-2,四钾盐是一种细胞内镁指示剂,具有比率和UV光可激发。 这种水溶性盐形式可用于通过微量注射或从贴片移液管输注来进行细胞内负载。 这种特殊的镁指示剂也用于高浓度钙检测。金畔生物是AAT Bioquest的中国代理商,为您提供最优质的钙离子荧光探针Mag-Fura-2 四钾盐 。 

点击查看光谱

钙离子篇:时间轴式讲解应用于钙离子检测的探针

 

参考文献

Tuning the Spectroscopic Properties of Ratiometric Fluorescent Metal Indicators: Experimental and Computational Studies on Mag-Fura-2 and Analogues
Authors: Zhang G, Jacquemin D, Buccella D.
Journal: J Phys Chem B. (2017)

Fluorescence measurements of free [Mg2+] by use of mag-fura 2 in Salmonella enterica
Authors: Froschauer EM, Kolisek M, Dieterich F, Schweigel M, Schweyen RJ.
Journal: FEMS Microbiol Lett (2004): 49

Ionic selectivity of low-affinity ratiometric calcium indicators: mag-Fura-2, Fura-2FF and BTC
Authors: Hyrc KL, Bownik JM, Goldberg MP.
Journal: Cell Calcium (2000): 75

Characterization of Mg2+ transport in brush border membrane vesicles of rabbit ileum studied with mag-fura-2
Authors: Juttner R, Ebel H.
Journal: Biochim Biophys Acta (1998): 51

Intracellular Mg2+ regulation in voltage-clamped Helix aspersa neurones measured with mag-fura-2 and Mg(2+)-sensitive microelectrodes
Authors: Kennedy HJ.
Journal: Exp Physiol (1998): 449

Mag-Fura-2 (Furaptra) exhibits both low (microM) and high (nM) affinity for Ca2+
Authors: Martinez-Zaguilan R, Parnami J, Martinez GM.
Journal: Cell Physiol Biochem (1998): 158

Monitoring of Ca2+ release from intracellular stores in permeabilized rat parotid acinar cells using the fluorescent indicators Mag-fura-2 and calcium green C18
Authors: Tojyo Y, Tanimura A, Matsumoto Y.
Journal: Biochem Biophys Res Commun (1997): 189

Effects of monovalent cations, pH and temperature on the dissociation constant (KD) for the fluorescent indicator mag-fura-2 at different excitation wavelengths
Authors: Howarth FC, Singh J, Waring JJ, Hustler BI, Bailey M.
Journal: Magnes Res (1995): 299

Intrasynaptosomal free Mg2+ concentration measured with the fluorescent indicator mag-fura-2: modulation by Na+ gradient and by extrasynaptosomal ATP
Authors: Garcia-Martin E, Martin-Romero FJ, Gutierrez-Merino C.
Journal: J Neurochem (1995): 2757

Determination of cytosolic Mg2+ activity and buffering in BC3H-1 cells with mag-fura-2
Authors: Grubbs RD, Walter A.
Journal: Mol Cell Biochem (1994): 11

说明书
钙离子荧光探针Mag-Fura-2 四钾盐 CAS 132319-57-4.pdf

钙离子荧光探针Mag-Fluo-4 钾盐 货号20400-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。

钙离子荧光探针Mag-Fluo-4 钾盐

钙离子荧光探针Mag-Fluo-4 钾盐

钙离子荧光探针Mag-Fluo-4 钾盐    货号20400 货号 20400 存储条件 在零下15度以下保存, 避免光照
规格 10×50 ug 价格 1944
Ex (nm) 495 Em (nm) 528
分子量 681.77 溶剂 Water
产品详细介绍

简要概述

产品基本信息
产品编号:20400
产品名称:钙离子荧光探针Mag-Fluo-4 钾盐
规格:10×50 ug
保质期:收到后12个月

化学和光谱特性
分子量:681.77
溶剂:水
激发波长:494
发射波长:516

 

产品介绍

钙离子荧光探针Mag-Fluo-4 钾盐是美国AAT Bioquest生产的钙离子荧光探针,不受细胞渗透的Mag-Fluo-4是Fluo-4的类似物,Md离子的Kd为4.7 mM,Ca离子的Kd为22 µM,使其可用作细胞内Mg离子指示剂以及低剂量的Mg离子指示剂。亲和钙离子指示剂。金畔生物是AAT Bioquest的中国代理商,为您提供最优质的钙离子荧光探针Mag-Fluo-4 钾盐。 

点击查看光谱

钙离子篇:时间轴式讲解应用于钙离子检测的探针

 

参考文献

Real-time intra-store confocal Ca2+ imaging in isolated mouse cardiomyocytes
Authors: Fernandez-Tenorio M, Niggli E.
Journal: Cell Calcium. (2016)

Redistribution of subcellular calcium and its effect on apoptosis in primary cultures of rat proximal tubular cells exposed to lead
Authors: Wang H, Wang ZK, Jiao P, Zhou XP, Yang DB, Wang ZY, Wang L.
Journal: Toxicology (2015): 137

Role of Mitofusin-2 in mitochondrial localization and calcium uptake in skeletal muscle
Authors: Ainbinder A, Boncompagni S, Protasi F, Dirksen RT.
Journal: Cell Calcium (2015): 14

EGF stimulates Mg(2+) influx in mammary epithelial cells
Authors: Trapani V, Arduini D, Luongo F, Wolf FI.
Journal: Biochem Biophys Res Commun (2014): 572

Tetanic Ca2+ transient differences between slow- and fast-twitch mouse skeletal muscle fibres: a comprehensive experimental approach
Authors: Calderon JC, Bolanos P, Caputo C.
Journal: J Muscle Res Cell Motil (2014): 279

TRPC1 is involved in Ca(2)(+) influx and cytotoxicity following Pb(2)(+) exposure in human embryonic kidney cells
Authors: Zhang H, Li W, Xue Y, Zou F.
Journal: Toxicol Lett (2014): 52

High-throughput functional assays of IP3-evoked Ca2+ release
Authors: Tovey SC, Taylor CW.
Journal: Cold Spring Harb Protoc (2013): 930

Measurement and simulation of myoplasmic calcium transients in mouse slow-twitch muscle fibres
Authors: Hollingworth S, Kim MM, Baylor SM.
Journal: J Physiol (2012): 575

Presence of store-operated Ca2+ entry in C57BL/6J mouse ventricular myocytes and its suppression by sevoflurane
Authors: Kojima A, Kitagawa H, Omatsu-Kanbe M, Matsuura H, Nosaka S.
Journal: Br J Anaesth (2012): 352

Sevoflurane protects ventricular myocytes from Ca2+ paradox-mediated Ca2+ overload by blocking the activation of transient receptor potential canonical channels
Authors: Kojima A, Kitagawa H, Omatsu-Kanbe M, Matsuura H, Nosaka S.
Journal: Anesthesiology (2011): 509

说明书
钙离子荧光探针Mag-Fluo-4 钾盐.pdf

钙离子荧光探针Mag-520 AM 货号20406-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。

钙离子荧光探针Mag-520 AM

钙离子荧光探针Mag-520 AM

钙离子荧光探针Mag-520 AM    货号20406 货号 20406 存储条件 在零下15度以下保存, 避免光照
规格 10×50 ug 价格 3924
Ex (nm) 506 Em (nm) 525
分子量 579.46 溶剂 DMSO
产品详细介绍

简要概述

产品基本信息

货号:20406

产品名称:钙离子荧光探针Mag-520 AM

规格:10x50ug

储存条件:-15℃避光防潮

保质期:24个月

 

产品物理化学光谱特性

分子量:579.46

外观:固体

溶剂:DMSO

激发波长(nm):N/A

发射波长(nm):N/A

 

适用仪器


流式细胞仪  
激发: 488nm激光
发射: 530/30nm滤波片
通道: FITC通道
荧光显微镜  
激发: FITC滤波片
发射: FITC滤波片
推荐孔板: 黑色透明

 

产品介绍

目前市场现售的绝大多数镁离子指示剂都是基于三羧酸-APTRA螯合剂,该螯合剂来源于四羧酸-BAPTA-钙离子螯合剂。它们包括mag-fura-2、mag-indo-1、mag-fluo-4和mag-rhod-3。虽然它们都是为检测镁离子而设计的,但它们对钙的亲和力都高于镁。Mag-520 是第一个对镁的亲和力高于钙的商用镁指示剂。与其他商业镁离子指示剂(如流行的mag-fluo-4)相比,其用于镁离子测量的选择性明显提高,而不受钙离子的干扰。Mag-520 AM具有细胞渗透性,其光谱与FITC几乎相同。金畔生物是AAT Bioquest的中国代理商,为您提供最优质的钙离子荧光探针Mag-520 AM。 

钙离子篇:时间轴式讲解应用于钙离子检测的探针

 

参考文献

Fluorescence lifetime imaging of intracellular magnesium content in live cells
Authors: Sargenti, A., C and eo, A., Farruggia, G., D’Andrea, C., Cappadone, C., Malucelli, E., Valentini, G., Taroni, P., Iotti, S.
Journal: Analyst (2019): 1876-1880

Synthesis of a highly Mg(2+)-selective fluorescent probe and its application to quantifying and imaging total intracellular magnesium
Authors: Sargenti, A., Farruggia, G., Zaccheroni, N., Marraccini, C., Sgarzi, M., Cappadone, C., Malucelli, E., Procopio, A., Prodi, L., Lombardo, M., Iotti, S.
Journal: Nat Protoc (2017): 461-471

A novel fluorescent chemosensor allows the assessment of intracellular total magnesium in small samples
Authors: Sargenti, A., Farruggia, G., Malucelli, E., Cappadone, C., Merolle, L., Marraccini, C., Andreani, G., Prodi, L., Zaccheroni, N., Sgarzi, M., Trombini, C., Lombardo, M., Iotti, S.
Journal: Analyst (2014): 1201-7

Intracellular magnesium detection by fluorescent indicators
Authors: Trapani, V., Schweigel-Rontgen, M., Cittadini, A., Wolf, F. I.
Journal: Methods Enzymol (2012): 421-44

Development of fluorescent probes for intracellular magnesium measurement and their applications to analysis of cellular mechanism
Authors: Shindo, Y., Oka, K., Komatsu, H., Suzuki, K.
Journal: Tanpakushitsu Kakusan Koso (2007): 1552-7

Mitochondria are intracellular magnesium stores: investigation by simultaneous fluorescent imagings in PC12 cells
Authors: Kubota, T., Shindo, Y., Tokuno, K., Komatsu, H., Ogawa, H., Kudo, S., Kitamura, Y., Suzuki, K., Oka, K.
Journal: Biochim Biophys Acta (2005): 19-28

Design and synthesis of highly sensitive and selective fluorescein-derived magnesium fluorescent probes and application to intracellular 3D Mg2+ imaging
Authors: Komatsu, H., Iwasawa, N., Citterio, D., Suzuki, Y., Kubota, T., Tokuno, K., Kitamura, Y., Oka, K., Suzuki, K.
Journal: J Am Chem Soc (2004): 16353-60

Investigation of intracellular magnesium mobilization pathways I PC12 cells B simultaneous Mg-Ca fluorescent imaging
Authors: Kubota, T., Shindo, Y., Tokuno, K., Komatsu, H., Ogawa, H., Kitamura, Y., Suzuki, K., Oka, K.
Journal: J Am Coll Nutr (2004): 742S-4S

Fluorescent monitoring of Jurkatt cell intracellular magnesium during metabolic poisoning
Authors: Carroll, R. G., Thomas, J. M.
Journal: Shock (1994): 213-6

Measurement of intracellular magnesium concentration in 3T3 fibroblasts with the fluorescent indicator Mag-indo-1
Authors: Morelle, B., Salmon, J. M., Vigo, J., Viallet, P.
Journal: Anal Biochem (1994): 170-6

说明书
钙离子荧光探针Mag-520 AM.pdf

钙离子荧光探针Mag-Fura-2, AM CAS 132319-57-4 货号20383-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。

钙离子荧光探针Mag-Fura-2, AM CAS 132319-57-4

钙离子荧光探针Mag-Fura-2, AM CAS 132319-57-4

钙离子荧光探针Mag-Fura-2, AM  CAS 132319-57-4    货号20383 货号 20383 存储条件 在零下15度以下保存, 避免光照
规格 10×50 ug 价格 2604
Ex (nm) 336 Em (nm) 505
分子量 722.56 溶剂 DMSO
产品详细介绍

简要概述

产品基本信息

货号:20383

产品名称:钙离子荧光探针Mag-Fura-2, AM 

CAS:132319-57-4

规格:10x50ug

储存条件:-15℃避光防潮

保质期:12个月

化学和光谱特性
分子量:722.56
溶剂:DMSO
激发波长:336
发射波长:503

 

适用仪器


荧光显微镜  
激发: Fura 2滤波片
发射: Fura 2滤波片
通道: 黑色透明
荧光酶标仪  
激发: 340,380nm
发射: 510nm
cutoff: 475nm
推荐孔板: 黑色透明
读取模式 底读模式

 

产品介绍

钙离子荧光探针Mag-Fura-2, AM 是美国AAT Bioquest生产的钙离子荧光探针,Mag-Fura-2,AM是一种细胞内镁指示剂,它是成比例的且可被紫外线激发。它的光谱特性与Fura-2非常匹配。这种乙酰氧基甲基(AM)酯形式可用于无创性细胞内装载。它也用于测量活细胞中的高水平钙离子。金畔生物是AAT Bioquest的中国代理商,为您提供最优质的钙离子荧光探针Mag-Fura-2, AM 。 

点击查看光谱

点击查看实验方案

钙离子篇:时间轴式讲解应用于钙离子检测的探针

 

参考文献

Tuning the Spectroscopic Properties of Ratiometric Fluorescent Metal Indicators: Experimental and Computational Studies on Mag-Fura-2 and Analogues
Authors: Zhang G, Jacquemin D, Buccella D.
Journal: J Phys Chem B. (2017)

Fluorescence measurements of free [Mg2+] by use of mag-fura 2 in Salmonella enterica
Authors: Froschauer EM, Kolisek M, Dieterich F, Schweigel M, Schweyen RJ.
Journal: FEMS Microbiol Lett (2004): 49

Ionic selectivity of low-affinity ratiometric calcium indicators: mag-Fura-2, Fura-2FF and BTC
Authors: Hyrc KL, Bownik JM, Goldberg MP.
Journal: Cell Calcium (2000): 75

Characterization of Mg2+ transport in brush border membrane vesicles of rabbit ileum studied with mag-fura-2
Authors: Juttner R, Ebel H.
Journal: Biochim Biophys Acta (1998): 51

Intracellular Mg2+ regulation in voltage-clamped Helix aspersa neurones measured with mag-fura-2 and Mg(2+)-sensitive microelectrodes
Authors: Kennedy HJ.
Journal: Exp Physiol (1998): 449

Mag-Fura-2 (Furaptra) exhibits both low (microM) and high (nM) affinity for Ca2+
Authors: Martinez-Zaguilan R, Parnami J, Martinez GM.
Journal: Cell Physiol Biochem (1998): 158

Monitoring of Ca2+ release from intracellular stores in permeabilized rat parotid acinar cells using the fluorescent indicators Mag-fura-2 and calcium green C18
Authors: Tojyo Y, Tanimura A, Matsumoto Y.
Journal: Biochem Biophys Res Commun (1997): 189

Effects of monovalent cations, pH and temperature on the dissociation constant (KD) for the fluorescent indicator mag-fura-2 at different excitation wavelengths
Authors: Howarth FC, Singh J, Waring JJ, Hustler BI, Bailey M.
Journal: Magnes Res (1995): 299

Intrasynaptosomal free Mg2+ concentration measured with the fluorescent indicator mag-fura-2: modulation by Na+ gradient and by extrasynaptosomal ATP
Authors: Garcia-Martin E, Martin-Romero FJ, Gutierrez-Merino C.
Journal: J Neurochem (1995): 2757

Determination of cytosolic Mg2+ activity and buffering in BC3H-1 cells with mag-fura-2
Authors: Grubbs RD, Walter A.
Journal: Mol Cell Biochem (1994): 11

说明书
钙离子荧光探针Mag-Fura-2, AM CAS 132319-57-4.pdf