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TAQuest FAST qPCR Master Mix 用于TaqMan探针*无ROX* 货号17288-AAT Bioquest荧光染料

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上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品，欢迎访问AAT Bioquest荧光染料官网了解更多信息。

TAQuest FAST qPCR Master Mix 用于TaqMan探针*无ROX*

TAQuest FAST qPCR Master Mix 用于TaqMan探针*无ROX*

TAQuest FAST qPCR Master Mix 用于TaqMan探针*无ROX* 货号17288 货号 17288 存储条件 在零下15度以下保存, 避免光照
规格 1 mL 价格 1164
Ex (nm) Em (nm)
分子量 溶剂 Water
产品详细介绍

简要概述

产品基本信息

货号：17288

产品名称：TAQuest FAST qPCR Master Mix 用于TaqMan探针*无ROX*

规格：1ml

储存条件：-15℃避光防潮

保质期：12个月

 

产品物理化学光谱特性

溶剂：水

 

产品介绍

用于 TaqMan 探针的 TAQuest FAST qPCR Master Mix 是一种即用型 2X 溶液，针对 qPCR 和两步法 RT-qPCR 进行了优化，非常适合用于 TaqMan 基因表达分析。预混液与 FAST 条件兼容，因此在 20 uL 反应体积中进行 40 个 PCR 循环，可在 50 分钟内提供结果。预混液提供所有基本成分，包括我们专有的 TAQuest FAST 热启动 Taq DNA 聚合酶和优化的 PCR 缓冲液中的 dNTP，但模板、引物和探针除外。用于 TaqMan 探针的 TAQuest FAST qPCR Master Mix 设计用于使用具有卓越性能的内部阳性对照进行双重反应。预混液可确保所有样品类型（如基因组、质粒、病毒和 cDNA 模板）的 PCR 特异性和灵敏度。该预混液不含 ROX 参考染料。金畔生物是AAT Bioquest的中国代理商，为您提供最优质的TAQuest FAST qPCR Master Mix 用于TaqMan探针*无ROX*。 

 

适用仪器

qPCR  
仪器规格 基于探针的滤波片

 

样品实验方案
注意 在室温下解冻TAQuest FAST qPCR Master Mix 用于TaqMan探针*无ROX*。 使用前彻底涡旋 qPCR Master Mix。
1. 制备表 1 所示的下列反应混合物之一。
2. 轻轻涡旋混合试剂，然后短暂离心。
3. 在 qPCR 仪器中设置板并按表 2 所示操作。

 

表 1. 各反应每孔试剂组成

成分 体积 (25 µL/reaction) 体积 (50 µL/reaction) 最终浓度
TAQuest FAST qPCR Master Mix 用于TaqMan探针*无ROX* 12.5 µL 25 µL 1X
上游引物，10 µM 0.25-2.5 µL 0.5-5.0 µL 0.1-1.0 µM
下游引物，10 µM 0.25-2.5 µL 0.5-5.0 µL 0.1-1.0 µM
DNA模板 1-5 µL 1-5 µL 优化的浓度
无核酸酶水 25 µL 50     µL  

表 2. 热循环参数

范围 聚合酶激活 PCR (30-40个循环)
  Hold 变性 退火 延伸
温度 95 °C 95 °C 55-65 °C 68-72 °C
时间 (m:ss) 0:20 0:30 1:00 1:00

 

参考文献

Evaluation of the efficiency of TaqMan duplex real-time PCR assay for non-invasive pre-natal assessment of foetal sex in equine.
Authors: Kadivar, Ali and Rashidzadeh, Habiballah and Davoodian, Najmeh and Nazari, Hasan and Dehghani Tafti, Rohallah and Heidari Khoei, Heidar and Seidi Samani, Hasan and Modaresi, Jahangir and Ahmadi, Ebrahim
Journal: Reproduction in domestic animals = Zuchthygiene (2021): 287-291

Fast and Sensitive Real-Time PCR Detection of Major Antiviral-Drug Resistance Mutations in Chronic Hepatitis B Patients by Use of a Predesigned Panel of Locked-Nucleic-Acid TaqMan Probes.
Authors: Chu, Son V and Vu, Son T and Nguyen, Hang M and Le, Ngan T and Truong, Phuong T and Vu, Van T T and Phung, Thuy T B and Nguyen, Anh T V
Journal: Journal of clinical microbiology (2021): e0093621

Multiplex TaqMan Real-Time PCR Assay for Sensitive Detection of Two Weevil Species (Coleoptera: Curculionidae).
Authors: Aguirre, Carlos and Sánchez, Evelyn and Olivares, Natalia and Hinrichsen, Patricio
Journal: Journal of economic entomology (2021): 90-99

A TaqMan-based quantitative real-time PCR assay for identification of the goose circovirus.
Authors: Yang, Kan-Kan and Yin, Dong-Dong and Xu, Liang and Liang, Yue-Qiao and Tu, Jian and Song, Xiang-Jun and Shao, Ying and Liu, Hong-Mei and Qi, Ke-Zong
Journal: Molecular and cellular probes (2020): 101564

A development strategy to fast establish the Taqman qPCR based method to detect SNP mutations.
Authors: Jiang, Xiaohui and Xiang, Junbei and Wang, Ruifeng and Wan, Qian
Journal: Human cell (2020): 1331-1333

BlueTYPE – A low density TaqMan-RT-qPCR array for the identification of all 24 classical Bluetongue virus serotypes.
Authors: Ries, Christina and Beer, Martin and Hoffmann, Bernd
Journal: Journal of virological methods (2020): 113881

Brief comparative evaluation of six open one-step RT-qPCR mastermixes for the detection of SARS-CoV-2 RNA using a Taqman probe.
Authors: Haddar, Cyrille and Verhoeven, Paul O and Bourlet, Thomas and Pozzetto, Bruno and Pillet, Sylvie
Journal: Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology (2020): 104636

Development of a new TaqMan-based PCR assay for the specific detection and quantification of Simkania negevensis.
Authors: Baccari, Olfa and Elleuch, Jihen and Barkallah, Mohamed and Boukedi, Hanen and Ayed, Nourelhouda Ben and Hammami, Adnene and Fendri, Imen and Abdelkafi, Slim
Journal: Molecular and cellular probes (2020): 101645

One-step multiplex TaqMan probe-based method for real-time PCR detection of four canine diarrhea viruses.
Authors: Wang, Ruyi and Zhang, Wenyan and Ye, Rui and Pan, Zhongzhou and Li, Gairu and Su, Shuo
Journal: Molecular and cellular probes (2020): 101618

Quantification of Major Bacteria and Yeast Species in Kefir Consortia by Multiplex TaqMan qPCR.
Authors: Nejati, Fatemeh and Junne, Stefan and Kurreck, Jens and Neubauer, Peter
Journal: Frontiers in microbiology (2020): 1291

说明书
TAQuest FAST qPCR Master Mix 用于TaqMan探针*无ROX*.pdf

TAQuest qPCR Master Mix with Helixyte Green *高ROX* 货号17274-AAT Bioquest荧光染料

发表于由

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品，欢迎访问AAT Bioquest荧光染料官网了解更多信息。

TAQuest qPCR Master Mix with Helixyte Green *高ROX*

TAQuest qPCR Master Mix with Helixyte Green *高ROX*

货号 17274 存储条件 在零下15度以下保存, 避免光照
规格 1 mL 价格 1164
Ex (nm) Em (nm)
分子量 溶剂 Water
产品详细介绍

简要概述

产品基本信息

货号：17274

产品名称：TAQuest qPCR Master Mix with Helixyte Green *高ROX*

规格：1ml

储存条件：-15℃避光防潮

保质期：12个月

 

产品物理化学光谱特性

溶剂：水

 

产品介绍

TAQuest qPCR Master Mix with Helixyte Green 是一种即用型 2X溶液，针对 qPCR 和 2 步 RT-qPCR 进行了优化。预混液在优化的 PCR 缓冲液中包含我们专有的 TAQuest 热启动 Taq DNA 聚合酶和 dNTP。您只需添加模板和目标引物即可运行所需的 PCR 反应。热启动 Taq DNA 聚合酶允许您在室温下设置 PCR 反应，从而最大限度地减少非特异性产物的形成。该酶与优化的缓冲液结合使用，可确保对所有样品类型（如基因组、质粒、病毒和 cDNA 模板）的 PCR 特异性和灵敏度。 Helixyte Green 嵌入染料无需使用序列特异性探针即可快速检测和分析DNA。该预混液包含大量ROX 参考染料。金畔生物是AAT Bioquest的中国代理商，为您提供最优质的TAQuest qPCR Master Mix with Helixyte Green *高ROX*。 

 

适用仪器

qPCR  
仪器规格 SYBR Green 滤波片

 

样品实验方案
注意 在室温下用 Helixyte Green *高ROX* 解冻 TAQuest™ qPCR Master Mix。 使用前彻底涡旋 qPCR Master Mix。
1. 制备表 1 所示的下列反应混合物之一。
2. 轻轻涡旋混合试剂，然后短暂离心。
3. 在 qPCR 仪器中设置板并按表 2 所示操作。

 

表 1. 各反应每孔试剂组成

成分 体积 (25 µL/reaction) 体积 (50 µL/reaction) 最终浓度
TAQuest qPCR Master Mix with Helixyte Green *高 ROX* 12.5 µL 25 µL 1X
上游引物，10 µM 0.25-2.5 µL 0.5-5.0 µL 0.1-1.0 µM
下游引物，10 µM 0.25-2.5 µL 0.5-5.0 µL 0.1-1.0 µM
DNA模板 1-5 µL 1-5 µL 优化的浓度
无核酸酶水 25 µL 50     µL  

表 2. 热循环参数

范围 聚合酶激活 PCR (30-40个循环)
  Hold 变性 退火 延伸
温度 95 °C 95 °C 55-65 °C 68-72 °C
时间 (m:ss) 0:20 0:30 1:00 1:00

 

参考文献

Aligned Expression of IFI16 and STING Genes in RRMS Patients’ Blood.
Authors: Helbi, Sobhan and Ravanbakhsh, Behnam and Karimi, Mohammad and Kooti, Wesam and Jivad, Nahid
Journal: Endocrine, metabolic & immune disorders drug targets (2020): 878-886

SNPs and transcriptional activity of genes of innate and adaptive immunity at the maternal-fetal interface in woman with preterm labour, associated with preterm premature rupture of membranes.
Authors: Lyubomirskaya, Ekaterina S and Kamyshnyi, Alexandr M and Krut, Yuriy Ya and Smiianov, Vladyslav A and Fedoniuk, Larisa Ya and Romanyuk, Lidiya B and Kravets, Natalya Ya and Mochulska, Oksana M
Journal: Wiadomosci lekarskie (Warsaw, Poland : 1960) (2020): 25-30

Real-Time Reverse Transcription PCR as a Tool to Study Virulence Gene Regulation in Bacterial Pathogens.
Authors: Aviv, Gili and Gal-Mor, Ohad
Journal: Methods in molecular biology (Clifton, N.J.) (2018): 23-32

An improved RT-IPCR for detection of pyrene and related polycyclic aromatic hydrocarbons.
Authors: Meng, X Y and Li, Y S and Zhou, Y and Sun, Y and Qiao, B and Si, C C and Hu, P and Lu, S Y and Ren, H L and Liu, Z S and Qiu, H J and Liu, J Q
Journal: Biosensors & bioelectronics (2016): 194-199

Analysis of P. gingivalis, T. forsythia and S. aureus levels in edentulous mouths prior to and 6 months after placement of one-piece zirconia and titanium implants.
Authors: Siddiqi, Allauddin and Milne, Trudy and Cullinan, Mary P and Seymour, Gregory J
Journal: Clinical oral implants research (2016): 288-94

Real-time immuno-PCR for ultrasensitive detection of pyrene and other homologous PAHs.
Authors: Meng, X Y and Li, Y S and Zhou, Y and Zhang, Y Y and Qiao, B and Sun, Y and Yang, L and Hu, P and Lu, S Y and Ren, H L and Zhang, J H and Wang, X R and Liu, Z S
Journal: Biosensors & bioelectronics (2015): 42-7

Real-time polymerase chain reaction based on msa2c gene for detection of Babesia bovis.
Authors: Ramos, Carlos A N and Araújo, Flábio R and Souza, Ingrid I F and Bacanelli, G and Luiz, Hera L and Russi, Lívia S and Oliveira, Renato H M and Soares, Cleber O and Rosinha, Grácia M S and Alves, Leucio C
Journal: Veterinary parasitology (2011): 79-83

说明书
TAQuest qPCR Master Mix with Helixyte Green *高ROX*.pdf

TAQuest qPCR Master Mix with Helixyte Green *无 ROX* 货号17270-AAT Bioquest荧光染料

发表于由

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品，欢迎访问AAT Bioquest荧光染料官网了解更多信息。

TAQuest qPCR Master Mix with Helixyte Green *无 ROX*

TAQuest qPCR Master Mix with Helixyte Green *无 ROX*

TAQuest qPCR Master Mix with Helixyte Green *无 ROX* 货号17270 货号 17270 存储条件 在零下15度以下保存, 避免光照
规格 1 mL 价格 1164
Ex (nm) Em (nm)
分子量 溶剂 Water
产品详细介绍

简要概述

产品基本信息

货号：17270

产品名称：TAQuest qPCR Master Mix with Helixyte Green *无 ROX*

规格：1ml

储存条件：-15℃避光防潮

保质期：12个月

 

产品物理化学光谱特性

溶剂：水

 

产品介绍

TAQuest qPCR Master Mix with Helixyte Green 是一种即用型 2X溶液，针对 qPCR 和 2 步 RT-qPCR 进行了优化。预混液在优化的 PCR 缓冲液中包含我们专有的 TAQuest 热启动 Taq DNA 聚合酶和 dNTP。您只需添加模板和目标引物即可进行所需的 PCR 反应。热启动 Taq DNA 聚合酶允许您在室温下设置 PCR 反应，从而最大限度地减少非特异性产物的形成。该酶与优化的缓冲液结合使用，可确保对所有样品类型（如基因组、质粒、病毒和 cDNA 模板）的 PCR 特异性和灵敏度。 Helixyte Green 嵌入染料无需使用序列特异性探针即可快速检测和分析 DNA。该预混液不含 ROX 参考染料。金畔生物是AAT Bioquest的中国代理商，为您提供最优质的TAQuest qPCR Master Mix with Helixyte Green *无 ROX*。

 

适用仪器

qPCR  
仪器规格 SYBR Green 滤波片

 

样品实验方案
注意 在室温下用 Helixyte Green *无 ROX* 解冻 TAQuest™ qPCR Master Mix。 使用前彻底涡旋 qPCR Master Mix。
1. 制备表 1 所示的下列反应混合物之一。
2. 轻轻涡旋混合试剂，然后短暂离心。
3. 在 qPCR 仪器中设置板并按表 2 所示操作。

 

表 1. 各反应每孔试剂组成

成分 体积 (25 µL/reaction) 体积 (50 µL/reaction) 最终浓度
TAQuest qPCR Master Mix with Helixyte Green *无 ROX* 12.5 µL 25 µL 1X
上游引物，10 µM 0.25-2.5 µL 0.5-5.0 µL 0.1-1.0 µM
下游引物，10 µM 0.25-2.5 µL 0.5-5.0 µL 0.1-1.0 µM
DNA模板 1-5 µL 1-5 µL 优化的浓度
无核酸酶水 25 µL 50     µL  

表 2. 热循环参数

范围 聚合酶激活 PCR (30-40个循环)
  Hold 变性 退火 延伸
温度 95 °C 95 °C 55-65 °C 68-72 °C
时间 (m:ss) 0:20 0:30 1:00 1:00

 

参考文献

A SYBR Green I-based real-time polymerase chain reaction assay for detection and quantification of canine bufavirus.
Authors: Wang, Yong and Sun, Jianfei and Guo, Xu and Li, Wei and Zhang, Da and Liu, Guangqing and Zhou, Tianhong and Li, Yongdong
Journal: Molecular and cellular probes (2021): 101762

A duplex SYBR green I-based real-time polymerase chain reaction assay for concurrent detection of feline parvovirus and feline coronavirus.
Authors: Sun, Liting and Xu, Zhiqing and Wu, Junhuang and Cui, Yongqiu and Guo, Xu and Xu, Fazhi and Li, Yongdong and Wang, Yong
Journal: Journal of virological methods (2021): 114294

A new SYBR Green real-time PCR to detect SARS-CoV-2.
Authors: Marinowic, D R and Zanirati, G and Rodrigues, F V F and Grahl, M V C and Alcará, A M and Machado, D C and Da Costa, J C
Journal: Scientific reports (2021): 2224

A novel duplex SYBR Green real-time PCR with melting curve analysis method for beef adulteration detection.
Authors: Li, Jiapeng and Wei, Yixuan and Li, Jinchun and Liu, Ruixi and Xu, Suigen and Xiong, Suyue and Guo, Ya and Qiao, Xiaoling and Wang, Shouwei
Journal: Food chemistry (2021): 127932

A rapid and low-cost protocol for the detection of B.1.1.7 lineage of SARS-CoV-2 by using SYBR Green-based RT-qPCR.
Authors: Abdel Sater, Fadil and Younes, Mahmoud and Nassar, Hassan and Nguewa, Paul and Hamze, Kassem
Journal: Molecular biology reports (2021): 7243-7249

Design and characterization of a SYBR Green I-based melting curve method for investigation of HER2I655V polymorphism in breast cancer.
Authors: Desriani and Azamris and Ghaissani, Shabrina S and Kinanti, Senja R and Warisman, Muhammad A and Fitria, N
Journal: Journal, genetic engineering & biotechnology (2021): 6

Development and Validation of a SYBR Green Real Time PCR Protocol for Detection and Quantification of Nervous Necrosis Virus (NNV) Using Different Standards.
Authors: Olveira, José G and Souto, Sandra and Bandín, Isabel and Dopazo, Carlos P
Journal: Animals : an open access journal from MDPI (2021)

Development and application of SYBR Green Ⅰ real-time quantitative reverse transcription PCR assay for detection of swine Getah virus.
Authors: Xia, Yin-He and Shi, Zi-Cong and Wang, Xin-Wei and Li, Yong-Tao and Wang, Zeng and Chang, Hong-Tao and Liu, Hong-Ying and Chen, Lu and Wang, Chuan-Qing and Yang, Xia
Journal: Molecular and cellular probes (2021): 101730

Development of New PCR Assay with SYBR Green I for Detection of Mycoplasma, Acholeplasma, and Ureaplasma sp. in Cell Cultures.
Authors: Krzysztoń-Russjan, Jolanta and Chudziak, Jakub and Bednarek, Małgorzata and Anuszewska, Elżbieta Lidia
Journal: Diagnostics (Basel, Switzerland) (2021)

Development of SYBR Green I-based polymerase chain reaction for feline bocavirus 1 detection.
Authors: Wang, Yong and Li, Wei and Guo, Xu and Zhang, Da and Sun, Jianfei and Fu, Ziteng and Liu, Guangqing and Li, Yongdong and Jiang, Shudong
Journal: 3 Biotech (2021): 61

说明书
TAQuest qPCR Master Mix with Helixyte Green *无 ROX*.pdf

TAQuest FAST qPCR Master Mix 用于TaqMan探针*低ROX* 货号17291-AAT Bioquest荧光染料

发表于由

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品，欢迎访问AAT Bioquest荧光染料官网了解更多信息。

TAQuest FAST qPCR Master Mix 用于TaqMan探针*低ROX*

TAQuest FAST qPCR Master Mix 用于TaqMan探针*低ROX*

TAQuest FAST qPCR Master Mix 用于TaqMan探针*低ROX* 货号17291 货号 17291 存储条件 在零下15度以下保存, 避免光照
规格 5 mL 价格 3612
Ex (nm) Em (nm)
分子量 溶剂 Water
产品详细介绍

简要概述

产品基本信息

货号：17291

产品名称：TAQuest FAST qPCR Master Mix 用于TaqMan探针*低ROX*

规格：5ml

储存条件：-15℃避光防潮

保质期：12个月

 

产品物理化学光谱特性

溶剂：水

 

产品介绍

用于 TaqMan 探针的 TAQuest FAST qPCR Master Mix 是一种即用型 2X 溶液，针对 qPCR 和两步法 RT-qPCR 进行了优化，非常适合用于 TaqMan 基因表达分析。预混液与 FAST 条件兼容，因此在 20 uL 反应体积中进行 40 个 PCR 循环，可在 50 分钟内提供结果。预混液提供所有基本成分，包括我们专有的 TAQuest FAST 热启动 Taq DNA 聚合酶和优化的 PCR 缓冲液中的 dNTP，但模板、引物和探针除外。用于 TaqMan 探针的 TAQuest FAST qPCR Master Mix 设计用于使用具有卓越性能的内部阳性对照进行双重反应。预混液可确保所有样品类型（如基因组、质粒、病毒和 cDNA 模板）的 PCR 特异性和灵敏度。该预混液含少量 ROX 参考染料。金畔生物是AAT Bioquest的中国代理商，为您提供最优质的TAQuest FAST qPCR Master Mix 用于TaqMan探针*低ROX*。

 

适用仪器

qPCR  
仪器规格 基于探针的滤波片

 

样品实验方案
注意 在室温下解冻TAQuest FAST qPCR Master Mix 用于TaqMan探针*低ROX*。 使用前彻底涡旋 qPCR Master Mix。
1. 制备表 1 所示的下列反应混合物之一。
2. 轻轻涡旋混合试剂，然后短暂离心。
3. 在 qPCR 仪器中设置板并按表 2 所示操作。

 

表 1. 各反应每孔试剂组成

成分 体积 (25 µL/reaction) 体积 (50 µL/reaction) 最终浓度
TAQuest FAST qPCR Master Mix 用于TaqMan探针*低ROX* 12.5 µL 25 µL 1X
上游引物，10 µM 0.25-2.5 µL 0.5-5.0 µL 0.1-1.0 µM
下游引物，10 µM 0.25-2.5 µL 0.5-5.0 µL 0.1-1.0 µM
DNA模板 1-5 µL 1-5 µL 优化的浓度
无核酸酶水 25 µL 50     µL  

表 2. 热循环参数

范围 聚合酶激活 PCR (30-40个循环)
  Hold 变性 退火 延伸
温度 95 °C 95 °C 55-65 °C 68-72 °C
时间 (m:ss) 0:20 0:30 1:00 1:00

 

参考文献

Evaluation of the efficiency of TaqMan duplex real-time PCR assay for non-invasive pre-natal assessment of foetal sex in equine.
Authors: Kadivar, Ali and Rashidzadeh, Habiballah and Davoodian, Najmeh and Nazari, Hasan and Dehghani Tafti, Rohallah and Heidari Khoei, Heidar and Seidi Samani, Hasan and Modaresi, Jahangir and Ahmadi, Ebrahim
Journal: Reproduction in domestic animals = Zuchthygiene (2021): 287-291

Fast and Sensitive Real-Time PCR Detection of Major Antiviral-Drug Resistance Mutations in Chronic Hepatitis B Patients by Use of a Predesigned Panel of Locked-Nucleic-Acid TaqMan Probes.
Authors: Chu, Son V and Vu, Son T and Nguyen, Hang M and Le, Ngan T and Truong, Phuong T and Vu, Van T T and Phung, Thuy T B and Nguyen, Anh T V
Journal: Journal of clinical microbiology (2021): e0093621

Multiplex TaqMan Real-Time PCR Assay for Sensitive Detection of Two Weevil Species (Coleoptera: Curculionidae).
Authors: Aguirre, Carlos and Sánchez, Evelyn and Olivares, Natalia and Hinrichsen, Patricio
Journal: Journal of economic entomology (2021): 90-99

A TaqMan-based quantitative real-time PCR assay for identification of the goose circovirus.
Authors: Yang, Kan-Kan and Yin, Dong-Dong and Xu, Liang and Liang, Yue-Qiao and Tu, Jian and Song, Xiang-Jun and Shao, Ying and Liu, Hong-Mei and Qi, Ke-Zong
Journal: Molecular and cellular probes (2020): 101564

A development strategy to fast establish the Taqman qPCR based method to detect SNP mutations.
Authors: Jiang, Xiaohui and Xiang, Junbei and Wang, Ruifeng and Wan, Qian
Journal: Human cell (2020): 1331-1333

BlueTYPE – A low density TaqMan-RT-qPCR array for the identification of all 24 classical Bluetongue virus serotypes.
Authors: Ries, Christina and Beer, Martin and Hoffmann, Bernd
Journal: Journal of virological methods (2020): 113881

Brief comparative evaluation of six open one-step RT-qPCR mastermixes for the detection of SARS-CoV-2 RNA using a Taqman probe.
Authors: Haddar, Cyrille and Verhoeven, Paul O and Bourlet, Thomas and Pozzetto, Bruno and Pillet, Sylvie
Journal: Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology (2020): 104636

Development of a new TaqMan-based PCR assay for the specific detection and quantification of Simkania negevensis.
Authors: Baccari, Olfa and Elleuch, Jihen and Barkallah, Mohamed and Boukedi, Hanen and Ayed, Nourelhouda Ben and Hammami, Adnene and Fendri, Imen and Abdelkafi, Slim
Journal: Molecular and cellular probes (2020): 101645

One-step multiplex TaqMan probe-based method for real-time PCR detection of four canine diarrhea viruses.
Authors: Wang, Ruyi and Zhang, Wenyan and Ye, Rui and Pan, Zhongzhou and Li, Gairu and Su, Shuo
Journal: Molecular and cellular probes (2020): 101618

Quantification of Major Bacteria and Yeast Species in Kefir Consortia by Multiplex TaqMan qPCR.
Authors: Nejati, Fatemeh and Junne, Stefan and Kurreck, Jens and Neubauer, Peter
Journal: Frontiers in microbiology (2020): 1291

说明书
TAQuest FAST qPCR Master Mix 用于TaqMan探针*低ROX*.pdf

SYBR 染料 qPCR 校准板 *针对 ABI7500 快速 96 孔进行了优化* 货号67008-AAT Bioquest荧光染料

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SYBR 染料 qPCR 校准板 *针对 ABI7500 快速 96 孔进行了优化*

SYBR 染料 qPCR 校准板 *针对 ABI7500 快速 96 孔进行了优化*

SYBR 染料 qPCR 校准板 *针对 ABI7500 快速 96 孔进行了优化* 货号67008 货号 67008 存储条件 在零下15度以下保存, 避免光照
规格 1 Plate 价格 1140
Ex (nm) Em (nm)
分子量 溶剂 DMSO
产品详细介绍

简要概述

SYBR 染料 qPCR 校准板可用于维护配备 Fast 96 孔板的 7500 实时 PCR 系统。 对于大多数 qPCR 仪器，必要的校准应至少每六个月进行一次。 该校准板可以立即使用，无需任何额外的准备步骤。 qPCR 校准板可以通过更准确地表示实时实验中使用的荧光光谱来改善多路复用的 qPCR 结果。

 

参考文献

Combined evaluation of proliferation and apoptosis to calculate IC50 of VPA-induced PANC-1 cells and assessing its effect on the Wnt signaling pathway.
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Identification of Echinococcus granulosus Genotypes G1 and G3 by SNPs Genotyping Assays.
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Expression levels of serum circulating microRNAs in pediatric patients with ventricular and supraventricular arrhythmias.
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Journal: Advances in medical sciences (2021): 411-417
RPA-PCR couple: an approach to expedite plant diagnostics and overcome PCR inhibitors.
Authors: Munawar, Mustafa Ahmad and Martin, Frank and Toljamo, Anna and Kokko, Harri and Oksanen, Elina
Journal: BioTechniques (2020): 270-280
Rapid detection of porcine DNA in processed food samples using a streamlined DNA extraction method combined with the SYBR Green real-time PCR assay.
Authors: Tan, Lee Lee and Ahmed, Siti Aminah and Ng, Siew Kit and Citartan, Marimuthu and Raabe, Carsten A and Rozhdestvensky, Timofey S and Tang, Thean Hock
Journal: Food chemistry (2020): 125654
Expression of SARS-CoV-2 receptor ACE2 and TMPRSS2 in human primary conjunctival and pterygium cell lines and in mouse cornea.
Authors: Ma, Di and Chen, Chong-Bo and Jhanji, Vishal and Xu, Ciyan and Yuan, Xiang-Ling and Liang, Jia-Jian and Huang, Yuqiang and Cen, Ling-Ping and Ng, Tsz Kin
Journal: Eye (London, England) (2020): 1212-1219
Preoperative heat shock protein 47 levels identify colorectal cancer patients with lymph node metastasis and poor prognosis.
Authors: Mori, Koichiro and Toiyama, Yuji and Okugawa, Yoshinaga and Ichikawa, Takashi and Nagano, Yuka and Oki, Satoshi and Shimura, Tadanobu and Fujikawa, Hiroyuki and Hiro, Junichiro and Kobayash, Minako and Araki, Toshimitsu and Inoue, Yasuhiro and Mohri, Yasuhiko and Kusunoki, Masato
Journal: Oncology letters (2020): 333
Distribution of virulence genes in bacteremic methicillin-resistant Staphylococcus aureus isolates from various sources.
Authors: Wang, Fu-Der and Wu, Ping-Feng and Chen, Su-Jung
Journal: Journal of microbiology, immunology, and infection = Wei mian yu gan ran za zhi (2019): 426-432
Polycystic ovary syndrome dependency on mtDNA mutation; copy Number and its association with insulin resistance.
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Aberrant Expression of the miR-181b/miR-222 after Hematopoietic Stem Cell Transplantation in Patients with Acute Myeloid Leukemia.
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Journal: Indian journal of hematology & blood transfusion : an official journal of Indian Society of Hematology and Blood Transfusion (2019): 446-450

说明书
SYBR 染料 qPCR 校准板 *针对 ABI7500 快速 96 孔进行了优化*.pdf