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Aminopropargyl ddTTP [5-Propargylamino-2′,3′-dideoxyuridine-5′-triphosphate] CAS 114748-59-3
货号 | 17072 | 存储条件 | 在零下15度以下保存, 避免光照 | |
规格 | 10 umoles | 价格 | 11988 | |
Ex (nm) | Em (nm) | |||
分子量 | 593.13 | 溶剂 | DMF | |
产品详细介绍 |
简要概述
产品基本信息
货号:17072
产品名称:Aminopropargyl ddTTP [5-Propargylamino-2′,3′-dideoxyuridine-5′-triphosphate]
CAS:114748-59-3
规格:10 umoles
储存条件:-15℃避光防潮
保质期:24个月
产品物理化学光谱特性
分子量:593.13
外观:液体
溶剂:DMF
激发波长(nm):N/A
发射波长(nm):N/A
产品介绍
Sanger法是DNA测序中最早最靠谱的方法之一,DNA由四种脱氧核苷酸三磷酸(dNTP)合成。将每个新核苷酸添加到最后的dNTP的3′-OH基团中。可以将二脱氧胸苷三磷酸(ddTTPs)添加到正在生长的DNA链中,但是当它出现时,链延长会停止,因为下一个要连接的核苷酸没有3′-OH。待测序的DNA被制备成单链。该模板DNA带有大量dATP,dGTP,dCTP和dTTP的混合物。加入四种双脱氧核苷酸(ddATP,ddGTP,ddCTP和ddTTP)的混合物,每种混合物均以限量存在,并分别用发不同颜色的荧光的“标签”标记。因为所有四个正常核苷酸都存在,所以链延伸正常进行,直到偶然地DNA聚合酶插入ddNTP(而不是正常dNTP)。如果正常核苷酸与双脱氧形式的比率足够高,则在插入ddNTP之前,某些DNA链将成功添加数百个核苷酸,从而终止该过程。在孵育期结束时,片段的长度从最长到最短分离,一个核苷酸之间的差异足以使该链与下一较短和下一较长链分开。当被激光束照射时,四个DDNTP中的每一个都会发出不同的荧光,可以通过自动扫描仪打印输出序列。这些ddATP,ddGTP,ddCTP和ddTTP胺衍生物是开发Sanger测序试剂的重要组成部分。金畔生物是AAT Bioquest的中国代理商,为您提供最优质的Aminopropargyl ddTTP [5-Propargylamino-2′,3′-dideoxyuridine-5′-triphosphate]。
参考文献
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