Aminopropargyl ddCTP [5-Propargylamino-2′,3′-dideoxycytidine-5′-triphosphate] 货号17070-AAT Bioquest荧光染料

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Aminopropargyl ddCTP [5-Propargylamino-2′,3′-dideoxycytidine-5′-triphosphate]

Aminopropargyl ddCTP [5-Propargylamino-2′,3′-dideoxycytidine-5′-triphosphate]

Aminopropargyl ddCTP [5-Propargylamino-2',3'-dideoxycytidine-5'-triphosphate]    货号17070 货号 17070 存储条件 在零下15度以下保存, 避免光照
规格 10 umoles 价格 11988
Ex (nm) Em (nm)
分子量 592.15 溶剂 DMF
产品详细介绍

简要概述

产品基本信息

货号:17070

产品名称:Aminopropargyl ddCTP [5-Propargylamino-2′,3′-dideoxycytidine-5′-triphosphate]

CAS:114748-56-0

规格:10 umoles

储存条件:-15℃避光防潮

保质期:24个月

 

产品物理化学光谱特性

分子量:592.15

外观:液体

溶剂:DMF

激发波长(nm):N/A

发射波长(nm):N/A

 

产品介绍

Sanger法是DNA测序中最早最靠谱的方法之一,DNA由四种脱氧核苷酸三磷酸(dNTP)合成。将每个新核苷酸添加到最后的dNTP的3′-OH基团中。可以将二脱氧胸苷三磷酸(ddTTPs)添加到正在生长的DNA链中,但是当它出现时,链延长会停止,因为下一个要连接的核苷酸没有3′-OH。待测序的DNA被制备成单链。该模板DNA带有大量dATP,dGTP,dCTP和dTTP的混合物。加入四种双脱氧核苷酸(ddATP,ddGTP,ddCTP和ddTTP)的混合物,每种混合物均以限量存在,并分别用发不同颜色的荧光的“标签​​”标记。因为所有四个正常核苷酸都存在,所以链延伸正常进行,直到偶然地DNA聚合酶插入ddNTP(而不是正常dNTP)。如果正常核苷酸与双脱氧形式的比率足够高,则在插入ddNTP之前,某些DNA链将成功添加数百个核苷酸,从而终止该过程。在孵育期结束时,片段的长度从最长到最短分离,一个核苷酸之间的差异足以使该链与下一较短和下一较长链分开。当被激光束照射时,四个DDNTP中的每一个都会发出不同的荧光,可以通过自动扫描仪打印输出序列。这些ddATP,ddGTP,ddCTP和ddTTP胺衍生物是开发Sanger测序试剂的重要组成部分。金畔生物是AAT Bioquest的中国代理商,为您提供最优质的Aminopropargyl ddCTP [5-Propargylamino-2′,3′-dideoxycytidine-5′-triphosphate]。 

 

参考文献

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Analysis of processivity of mungbean dideoxynucleotide-sensitive DNA polymerase and detection of the activity and expression of the enzyme in the meristematic and meiotic tissues and following DNA damaging agent
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A dideoxynucleotide-sensitive DNA polymerase activity characterized from endoreduplicating cells of mungbean (Vigna radiata L.) during ontogeny of cotyledons
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Mechanism-based suppression of dideoxynucleotide resistance by K65R human immunodeficiency virus reverse transcriptase using an alpha-boranophosphate nucleoside analogue
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Synthesis of the first ferrocene-labeled dideoxynucleotide and its use for 3′-redox end-labeling of 5′-modified single-stranded oligonucleotides
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Journal: Bioconjug Chem (2001): 396-405

Improving dideoxynucleotide-triphosphate utilisation by the hyper-thermophilic DNA polymerase from the archaeon Pyrococcus furiosus
Authors: Evans, S. J., Fogg, M. J., Mamone, A., Davis, M., Pearl, L. H., Connolly, B. A.
Journal: Nucleic Acids Res (2000): 1059-66

Structure-based design of Taq DNA polymerases with improved properties of dideoxynucleotide incorporation
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Journal: Proc Natl Acad Sci U S A (1999): 9491-6

Characterization of the native and recombinant catalytic subunit of human DNA polymerase gamma: identification of residues critical for exonuclease activity and dideoxynucleotide sensitivity
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Journal: Biochemistry (1998): 10529-39

Comparative performance of high-density oligonucleotide sequencing and dideoxynucleotide sequencing of HIV type 1 pol from clinical samples
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Journal: AIDS Res Hum Retroviruses (1998): 869-76

说明书
Aminopropargyl ddCTP [5-Propargylamino-2′,3′-dideoxycytidine-5′-triphosphate].pdf