TAQuest qPCR Master Mix 用于TaqMan探针*高ROX* 货号17287-AAT Bioquest荧光染料

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TAQuest qPCR Master Mix 用于TaqMan探针*高ROX*

TAQuest qPCR Master Mix 用于TaqMan探针*高ROX*

货号 17287 存储条件 在零下15度以下保存, 避免光照
规格 5 mL 价格 3612
Ex (nm) Em (nm)
分子量 溶剂 Water




产品名称:TAQuest qPCR Master Mix 用于TaqMan探针*高ROX*









用于 TaqMan 探针的 TAQuest qPCR Master Mix 是一种即用型 2X溶液,针对 qPCR 和与 TaqMan 基因表达分析兼容的两步法 RT-qPCR 进行了优化。预混液在优化的 PCR 缓冲液中提供了所有基本成分,包括我们专有的 TAQuest 热启动 Taq DNA 聚合酶和 dNTP,但模板、引物和探针除外。热启动 Taq DNA 聚合酶允许您在室温下设置 PCR 反应,从而最大限度地减少非特异性产物的形成。优化的成分可确保 PCR 对所有样本类型(如基因组、质粒、病毒和 cDNA 模板)的特异性和灵敏度。用于 TaqMan 探针的 TAQuest qPCR Master Mix 设计用于使用内部阳性对照的双链反应。该预混液含大量 ROX 参考染料。金畔生物是AAT Bioquest的中国代理商,为您提供最优质的TAQuest qPCR Master Mix 用于TaqMan探针*高ROX*。 



仪器规格 基于探针的滤波片


注意 在室温下解冻TAQuest qPCR Master Mix 用于TaqMan探针*高ROX*。 使用前彻底涡旋 qPCR Master Mix。
1. 制备表 1 所示的下列反应混合物之一。
2. 轻轻涡旋混合试剂,然后短暂离心。
3. 在 qPCR 仪器中设置板并按表 2 所示操作。


表 1. 各反应每孔试剂组成

成分 体积 (25 µL/reaction) 体积 (50 µL/reaction) 最终浓度
TAQuest qPCR Master Mix 用于TaqMan探针*高ROX* 12.5 µL 25 µL 1X
上游引物,10 µM 0.25-2.5 µL 0.5-5.0 µL 0.1-1.0 µM
下游引物,10 µM 0.25-2.5 µL 0.5-5.0 µL 0.1-1.0 µM
DNA模板 1-5 µL 1-5 µL 优化的浓度
无核酸酶水 25 µL 50     µL  

表 2. 热循环参数

范围 聚合酶激活 PCR (30-40个循环)
  Hold 变性 退火 延伸
温度 95 °C 95 °C 55-65 °C 68-72 °C
时间 (m:ss) 0:20 0:30 1:00 1:00



Resilient SARS-CoV-2 diagnostics workflows including viral heat inactivation.
Authors: Lista, Maria Jose and Matos, Pedro M and Maguire, Thomas J A and Poulton, Kate and Ortiz-Zapater, Elena and Page, Robert and Sertkaya, Helin and Ortega-Prieto, Ana M and Scourfield, Edward and O’Byrne, Aoife M and Bouton, Clement and Dickenson, Ruth E and Ficarelli, Mattia and Jimenez-Guardeño, Jose M and Howard, Mark and Betancor, Gilberto and Galao, Rui Pedro and Pickering, Suzanne and Signell, Adrian W and Wilson, Harry and Cliff, Penelope and Kia Ik, Mark Tan and Patel, Amita and MacMahon, Eithne and Cunningham, Emma and Doores, Katie and Agromayor, Monica and Martin-Serrano, Juan and Perucha, Esperanza and Mischo, Hannah E and Shankar-Hari, Manu and Batra, Rahul and Edgeworth, Jonathan and Zuckerman, Mark and Malim, Michael H and Neil, Stuart and Martinez-Nunez, Rocio Teresa
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Frequency-encoded laser-induced fluorescence for multiplexed detection in infrared-mediated quantitative PCR.
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Real-time stability testing of air-dried primers and fluorogenic hydrolysis probes stabilized by trehalose and xanthan.
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Development of a novel internal positive control for Taqman based assays.
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[Multiplex polymerase chain reaction for genetically modified potato event AV43-6-G7 quantification. Proof of efficiency].
Authors: Tyshko, N V and Sadykova, E O and Sukhacheva, M V and Grouzdev, D S
Journal: Voprosy pitaniia: 62-70

TAQuest qPCR Master Mix 用于TaqMan探针*高ROX*.pdf