胱天蛋白酶Caspase抑制剂Z-LETD-FMK 货号13307-AAT Bioquest荧光染料

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胱天蛋白酶Caspase抑制剂Z-LETD-FMK

胱天蛋白酶Caspase抑制剂Z-LETD-FMK

胱天蛋白酶Caspase抑制剂Z-LETD-FMK    货号13307 货号 13307 存储条件 在零下15度以下保存, 避免光照
规格 1 mg 价格 1200
Ex (nm) Em (nm)
分子量 654.69 溶剂 DMSO
产品详细介绍

简要概述

Z-LETD-FMK是一种细胞渗透性不可逆的caspase蛋白酶8抑制剂,与caspase-8的催化位点结合,在诱导凋亡中起重要作用。Z-LETD-FMK与caspase-8的结合抑制了该蛋白酶的活性,使其能够抑制与caspase-8激活相关的凋亡事件。为了抑制细胞凋亡,应在诱导细胞凋亡的同时添加Z-LETD-FMK。FMK衍生的肽可作为有效的不可逆抑制剂,而不会增加细胞毒性作用。金畔生物是AAT Bioquest的中国代理商,为您提供最优质的Z-LETD-FMK。 

产品说明书

样品实验方案

以下是我们推荐的方案,仅提供指导。具体实验应根据您的特定需求进行修改。

 

溶液配制

储备溶液配制

Z-LETD-FMK储备溶液配制:在Z-LETD-FMK中添加适量的DMSO,制成2-5 mM Z-LETD-FMK储备液。注意:将未使用的Z-LETD-FMK储备溶液分装在-20°C下。

 

操作步骤

  1. 根据需要处理样品以诱导凋亡。
  2. 添加Z-LETD-FMK。
    注意:我们建议使用最终浓度为10-100 µM的Z-LETD-FMK抑制胱天蛋白酶,例如对caspase-8抑制50 µM。
    注意:孵育的最佳时间和浓度需要通过实验确定。
  3. 根据需要对样品染色。
  4. 用适当的仪器检测荧光强度。 

 

图示

 

 

胱天蛋白酶Caspase抑制剂Z-LETD-FMK    货号13307

图1.用caspase 8抑制剂Z-LETD-FMK(Cat#13307)抑制Jurkat细胞中caspase 8活性。当天,将Jurkat细胞以200,000个细胞/ 90 µL /孔的密度接种在Costar黑色96孔板中。用1 µM星形孢菌素处理细胞5小时。加入Z-LETD-FMK并与星形孢菌素共孵育。用Cell Meter Caspase 8活性细胞凋亡检测试剂盒(Cat#22816)检测caspase-8活性。用Flexstation 3荧光酶标仪(Molecular Devices)在Ex / Em = 540 / 620nm(截止= 610nm)下测量荧光强度。

 

 

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说明书
胱天蛋白酶Caspase抑制剂Z-LETD-FMK.pdf