钙离子荧光探针Quin-2, AM CAS 83104-85-2 货号21050-AAT Bioquest荧光染料

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钙离子荧光探针Quin-2, AM CAS 83104-85-2

钙离子荧光探针Quin-2, AM CAS 83104-85-2

钙离子荧光探针Quin-2, AM CAS 83104-85-2    货号21050 货号 21050 存储条件 在零下15度以下保存, 避免光照
规格 1 mg 价格 2328
Ex (nm) 353 Em (nm) 495
分子量 829.76 溶剂 DMSO
产品详细介绍

简要概述

产品基本信息

货号:21050

产品名称:钙离子荧光探针Quin-2, AM

CAS:83104-85-2

规格:1mg

储存条件:-15℃避光防潮

保质期:24个月

 

产品物理化学光谱特性

分子量:829.76

溶剂:DMSO

激发波长(nm):353

发射波长(nm):495

 

适用仪器


荧光酶标仪  
激发: 340nm
发射: 495nm
cutoff: 475nm
推荐孔板: 黑色透明
读取模式: 底读模式

 

产品介绍

钙离子荧光探针Quin-2, AM是美国AAT Bioquest生产的钙离子荧光探针,Quin-2与钙紧密结合,与钙螯合剂EGTA类似,与钙的结合能力比镁更紧密。钙的结合会导致紫外线吸收和荧光发生很大变化。钙结合时引起荧光的光的波长比不结合时引起荧光的光的波长长。当在两个不同的波长下激发时,在两个波长下的荧光强度之比即为与游离钙结合的浓度之比。可以精确测量游离Quin-2的浓度,因此可以精确计算出游离钙的浓度。可以将Quin-2注射到细胞中以测量细胞内钙浓度的瞬时变化。Quin-2 AM对细胞具有渗透性,并用于研究活细胞。金畔生物是AAT Bioquest的中国代理商,为您提供最优质的钙离子荧光探针Quin-2, AM。 

点击查看实验方案

钙离子篇:时间轴式讲解应用于钙离子检测的探针

 

参考文献

W-5 and quin 2-AM reverse the inhibitory effect of insulin on lipolysis due to dibutyryl cAMP
Authors: Goko H, Matsuoka A.
Journal: Diabetes Res Clin Pract (1999): 101

Calcium chelator Quin-2 prevents crocidolite-induced DNA strand breakage in human white blood cells
Authors: Faux SP, Michelangeli F, Levy LS.
Journal: Mutat Res (1994): 209

Fluorescence lifetime imaging of intracellular calcium in COS cells using Quin-2
Authors: Lakowicz JR, Szmacinski H, Nowaczyk K, Lederer WJ, Kirby MS, Johnson ML.
Journal: Cell Calcium (1994): 7

Possible mechanisms of epinephrine actions in quin-2-loaded platelets refractory to arachidonic acid
Authors: Rao GH, Gerrard JM, Murthy M, White JG.
Journal: Biochem Med Metab Biol (1993): 322

Fluorescence lifetime imaging of calcium using Quin-2
Authors: Lakowicz JR, Szmacinski H, Nowaczyk K, Johnson ML.
Journal: Cell Calcium (1992): 131

Involvement of calcium and iron in Quin 2 toxicity to isolated hepatocytes
Authors: Carpenter-Deyo L, Reed DJ.
Journal: J Pharmacol Exp Ther (1991): 747

Toxicity to isolated hepatocytes caused by the intracellular calcium indicator, Quin 2
Authors: Carpenter-Deyo L, Duimstra JR, Hedstrom O, Reed DJ.
Journal: J Pharmacol Exp Ther (1991): 739

Aspirin, prostaglandin E1 and Quin-2 AM-induced platelet dysfunction: restoration of function by noradrenalin
Authors: Rao GH, White JG.
Journal: Prostaglandins Leukot Essent Fatty Acids (1990): 141

Characterization of indo-1 and quin-2 as spectroscopic probes for Zn2(+)-protein interactions
Authors: Jefferson JR, Hunt JB, Ginsburg A.
Journal: Anal Biochem (1990): 328

Effect of Quin-2 on Ca2+ uptake mediated by Na+i/Ca2+o exchange and 45Ca2+ efflux in rat brain synaptosomes: a requirement for [Ca2+]i
Authors: Blanco P, Martinez-Serrano A, Bogonez E, Satrustegui J.
Journal: Cell Calcium (1990): 25

说明书
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