Amplite ADHP过氧化物和过氧化酶荧光探针 相当于Amplex Red 货号11000-AAT Bioquest荧光染料

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Amplite ADHP过氧化物和过氧化酶荧光探针 相当于Amplex Red

Amplite ADHP过氧化物和过氧化酶荧光探针 相当于Amplex Red

Amplite ADHP过氧化物和过氧化酶荧光探针 相当于Amplex Red     货号11000 货号 11000 存储条件 在零下15度以下保存, 避免光照
规格 25 mg 价格 2604
Ex (nm) 571 Em (nm) 584
分子量 257.24 溶剂 DMSO
产品详细介绍

简要概述

产品基本信息

货号:11000

产品名称:Amplite ADHP过氧化物和过氧化酶荧光探针

规格:25mg

储存条件:-15℃避光防潮

保质期:24个月

 

产品物理化学光谱特性

分子量:257.24

外观:白色固体

溶剂:DMSO

激发波长(nm):571

发射波长(nm):585

 

产品介绍

Amplite ADHP过氧化物和过氧化酶荧光探针是美国AAT Bioquest生产的过氧化氢酶荧光底物,Amplite ADHP与AmplexRed 在化学上是相同的(AmplexRed 是Invitrogen的商标)。它是一种敏感的荧光过氧化物酶底物,其背景比其他商业供应商的材料低得多。ADHP产生高荧光的试卤灵,其最大吸收为571nm,最大发射为585nm。与其他HRP底物如二氢荧光素和二氢罗丹明不同,ADHP的空气氧化是最小的。到目前为止,ADHP被认为是用于检测HRP和H2O2的最灵敏和最稳定的荧光探针。ADHP已被广泛用于检测许多免疫测定中的HRP。另一方面,ADHP也可用于检测痕量的H2O2。基于ADHP的H2O2检测比常用的硫氧还蛋白对H2O2的检测灵敏度至少高一个数量级。由于H2O2是在许多酶促氧化还原反应中产生的,因此ADHP可用于偶联酶促反应,以检测许多氧化酶和/或相关酶/底物或辅助因子如葡萄糖,乙酰胆碱和胆固醇,L-谷氨酸,氨基酸等的活性。简单地制备10-20mM DMSO储备溶液,然后稀释到测定缓冲液如PBS,Tris缓冲液中,用于测定50-200μM。金畔生物是AAT Bioquest的中国代理商,为您提供最优质的Amplite ADHP过氧化物和过氧化酶荧光探针。 

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参考文献

Multimodal Molecular Imaging Demonstrates Myeloperoxidase Regulation of Matrix Metalloproteinase Activity in Neuroinflammation
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Assessment of Tofacitinib and Ruxolitinib and their Anti Inflammatory Effects on Myeloperoxidase
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Spinal Cord Inflammation: Molecular Imaging after Thoracic Aortic Ischemia Reperfusion Injury
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Myeloperoxidase Nuclear Imaging for Epileptogenesis
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Myeloperoxidase–Hepatocyte–Stellate Cell Cross Talk Promotes Hepatocyte Injury and Fibrosis in Experimental Nonalcoholic Steatohepatitis
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Journal: Archives of biochemistry and biophysics (2014): 74–85

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Measuring myeloperoxidase activity in biological samples
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Journal: PLoS One (2013): e67976

Micro-volume wall-less immunoassays using patterned planar plates
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