Cell Meter VX500固定化细胞活性染料 货号22542-AAT Bioquest荧光染料

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Cell Meter VX500固定化细胞活性染料

Cell Meter VX500固定化细胞活性染料

Cell Meter VX500固定化细胞活性染料    货号22542 货号 22542 存储条件 在零下15度以下保存, 避免光照
规格 200 Tests 价格 2472
Ex (nm) 433 Em (nm) 498
分子量 溶剂 DMSO
产品详细介绍

简要概述

产品基本信息

货号:22542

产品名称:Cell Meter VX500固定化细胞活性染料

规格:200 Tests

储存条件:保存在冰箱-15℃干燥

保质期:12个月

 

产品物理化学光谱特性

分子量:N/A

激发波长(nm):433

发射波长(nm):498

 

适用仪器


流式细胞仪  
激发: 405 nm激光
发射: 525/50 nm滤波片
滤波片: Pacific Orange滤波片组

 

产品介绍

从活细胞中区分和排除死细胞可以更清晰地分离和鉴定细胞群。Cell Meter VX500固定化细胞活性染料是一类细胞不可渗透的荧光活性染料,它们经过优化以匹配常见流式细胞仪的主要激发激光,例如350、405、488、633和647 nm。这些染料不渗入活细胞,但渗入膜受损的细胞。它们与含胺和硫醇的蛋白质以及其他细胞成分发生不可逆的反应。由于具有受损膜的死细胞或固定细胞更容易与Cell Meter VX500固定化细胞活性染料发生反应,因此比具有完整膜的活细胞的染色更亮,因此这些染料可用于评估哺乳动物细胞的活与死状态。使用这些染料时,需要考虑一些因素,例如,每种染料的滴定,以确保活细胞几乎没有染色。Cell Meter VX500固定化细胞活性染料经过优化,可以在405 nm的紫色激光激发下以500 nm的波长发射。与其他商业上类似的活性染料相比,这种固定化细胞活性染料更加耐用、稳定。金畔生物是AAT Bioquest的中国代理商,为您提供最优质的Cell Meter VX500固定化细胞活性染料。 

点击查看光谱

 

图示

 

 

Cell Meter VX500固定化细胞活性染料    货号22542

图1.通过Cell Meter 固定化活性染料检测Jurkat细胞活性。处理Jurkat细胞并用Cell Meter VX500(Cat#22542)染色,然后固定在3.7%甲醛中,并通过流式细胞仪进行分析。死细胞群体(蓝色峰)与带有太平洋橙色通道的活细胞群体(红色峰)很容易区分开,在固定之前和之后获得几乎相同的结果。

 

 

参考文献

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Journal: Anticancer research (2020): 653-664

The miR-3940-5p inhibits cell proliferation of gingival mesenchymal stem cells.
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Gag-Specific CD4 T Cell Proliferation, Plasmacytoid Dendritic Cells, and Ethnicity in Perinatally HIV-1-Infected Youths: The ANRS-EP38-IMMIP Study.
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A comparative study of colorimetric cell proliferation assays in immune cells.
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Hypoxia inhibits mesenchymal stem cell proliferation through HIF1α-dependent regulation of P27.
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Journal: Molecular and cellular biochemistry (2016): 29-38

说明书
Cell Meter VX500固定化细胞活性染料.pdf

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