Cell Meter 细胞凋亡活性检测试剂盒*红色荧光* 货号23007-AAT Bioquest荧光染料

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Cell Meter 细胞凋亡活性检测试剂盒*红色荧光*

Cell Meter 细胞凋亡活性检测试剂盒*红色荧光*

Cell Meter 细胞凋亡活性检测试剂盒*红色荧光*    货号23007 货号 23007 存储条件 Multiple
规格 100 Tests 价格 4368
Ex (nm) 544 Em (nm) 567
分子量 溶剂
产品详细介绍

简要概述

产品基本信息

货号:23007

产品名称:Cell Meter 细胞凋亡活性检测试剂盒

规格:100 Tests

储存条件:保存在冰箱-15℃干燥

保质期:12个月

 

产品物理化学光谱特性

分子量:N/A

溶剂:DMSO

激发波长(nm):N/A

发射波长(nm):N/A

 

适用仪器


流式细胞仪  
激发: 480nm激光
发射: 575/26nm滤波片
通道: PE通道
荧光显微镜  
激发: Cy3/TRITC滤波片组
发射: Cy3/TRITC滤波片组
推荐孔板: 黑色透明

 

产品介绍

细胞衰老是触发不可逆的生长停滞,以防止DNA损伤的细胞生长。衰老相关的β-半乳糖苷酶在衰老细胞中高度过表达,已被广泛用作衰老标记。比色X-gal染色法广泛用于检测衰老细胞中的SA-beta-gal。但是,着色方法有一些局限性,例如需要固定细胞(由于X-gal的细胞通透性低),染色时间较长和灵敏度低。Cell Meter 细胞凋亡活性检测试剂盒使用红色β-D-吡喃半乳糖苷,一种荧光性β-Gal底物,易于进入活细胞,并被细胞内的β-半乳糖苷酶裂解,产生强烈的红色荧光。与不渗透细胞的X-Gal底物不同,它具有出色的细胞渗透性。强大的Cell Meter 细胞凋亡活性检测试剂盒使用户能够以更高的灵敏度检测衰老。Xite Redβ-D-吡喃半乳糖苷也可以固定用于进一步的细胞分析。Xite Red的红色荧光可以很容易地与其他彩色荧光探针(例如DAPI或GFP)结合使用。Xite Red产品可以很好地保留在细胞内部,为荧光成像和流式细胞仪分析产生稳定的信号。

点击查看光谱

 

图示

 

Cell Meter 细胞凋亡活性检测试剂盒*红色荧光*    货号23007

图1.使用NovoCyte流式细胞仪(ACEA Biosciences),用Cell Meter 细胞凋亡活性检测试剂盒进行固定检测。将9L-LacZ细胞与DMSO或Xite Redβ-D-吡喃半乳糖苷在37°C下孵育45分钟。使用PE通道获取固定之前和之后的信号。(然后将细胞在室温下用4%甲醛固定20分钟,然后清洗一次。)

Cell Meter 细胞凋亡活性检测试剂盒*红色荧光*    货号23007

图2. 9L-LacZ细胞的荧光图像。将9L-LacZ细胞直接用Xite Redβ-D-吡喃半乳糖苷在37°C的细胞培养基中染色45分钟。使用Cy3 / TRITC滤波器组收集信号。

 

参考文献

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Pyrenebutyrate Leads to Cellular Binding, Not Intracellular Delivery, of Polyarginine-Quantum Dots.
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An improved cell-penetrating, caspase-activatable, near-infrared fluorescent peptide for apoptosis imaging.
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