FAM-xtra 亚磷酰胺

	货号	6038	存储条件	在零下15度以下保存, 避免光照
	规格	100 umoles	价格	1776
	Ex (nm)	493	Em (nm)	517
	分子量	879.94	溶剂	MeCN
	产品详细介绍

简要概述

产品基本信息

货号：6038

产品名称：FAM-xtra 亚磷酰胺

规格：100 umoles

储存条件：-15℃避光防潮

保质期：12个月

产品物理化学光谱特性

分子量：879.94

溶剂：MeCN

产品介绍

6-FAM亚磷酰胺可能是最流行的荧光构建基块，已广泛用于通过荧光素荧光团有效标记5’端的寡核苷酸。标准裂解和氢氧化铵脱保护用于释放荧光素标记的寡核苷酸。尽管6-FAM已普遍用于开发各种qPCR探针，但其荧光受到两个作用限制：1.高度依赖pH值；2.光漂白迅速。目前我们已开发出FAM-xtra 亚磷酰胺解决了这两个局限性，同时完整保留了6-FAM亚磷酰胺的所有优点。 FAM-xtra亚磷酰胺可在相同操作条件下与6-FAM亚磷酰胺完全一样​​使用，而无需进行任何更改。由FAM-xtra亚磷酰胺制成的寡核苷酸具有许多优点：1.FAM-xtra标记的寡核苷酸具有与6-FAM标记的寡核苷酸相同的光谱。 2.FAM-xtra标记的寡核苷酸比相应的FAM-标记的寡核苷酸明亮得多。 3.FAM-xtra标记的寡核苷酸比相应的FAM-标记的寡核苷酸具有更高的光稳定性。4.与相应的FAM标记的寡核苷酸相比，FAM-xtra标记的寡核苷酸的荧光对pH的敏感度低得多。在生理条件下，FAM-xtra标记的寡核苷酸的荧光基本上与pH无关，这使得FAM-xtra标记的寡核苷酸的qPCR探针更加稳定。

参考文献

Development of an Innovative Intradermal siRNA Delivery System Using a Combination of a Functional Stearylated Cytoplasm-Responsive Peptide and a Tight Junction-Opening Peptide.
Authors: Ibaraki, Hisako and Kanazawa, Takanori and Takashima, Yuuki and Okada, Hiroaki and Seta, Yasuo
Journal: Molecules (Basel, Switzerland) (2016)

Non-nucleoside phosphoramidites of xanthene dyes (FAM, JOE, and TAMRA) for oligonucleotide labeling.
Authors: Kvach, Maksim V and Tsybulsky, Dmitry A and Shmanai, Vadim V and Prokhorenko, Igor A and Stepanova, Irina A and Korshun, Vladimir A
Journal: Current protocols in nucleic acid chemistry (2013): Unit 4.55

Practical synthesis of isomerically pure 5- and 6-carboxytetramethylrhodamines, useful dyes for DNA probes.
Authors: Kvach, Maksim V and Stepanova, Irina A and Prokhorenko, Igor A and Stupak, Aleksander P and Bolibrukh, Dmitry A and Korshun, Vladimir A and Shmanai, Vadim V
Journal: Bioconjugate chemistry (2009): 1673-82

Synthesis and cellular uptake of fluorescently labeled multivalent hyaluronan disaccharide conjugates of oligonucleotide phosphorothioates.
Authors: Karskela, Marika and Virta, Pasi and Malinen, Melina and Urtti, Arto and Lönnberg, Harri
Journal: Bioconjugate chemistry (2008): 2549-58

Synthesis of HyBeacons and dual-labelled probes containing 2′-fluorescent groups for use in genetic analysis.
Authors: Dobson, Neil and McDowell, David G and French, David J and Brown, Lynda J and Mellor, John M and Brown, Tom
Journal: Chemical communications (Cambridge, England) (2003): 1234-5

Amplified fragment length polymorphism (AFLP) protocol for genotyping the malarial parasite Plasmodium falciparum.
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Journal: Parasitology (2001): 331-6

Recovery of an oligonucleotide using silver ions immobilized onto paramagnetic particles.
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Fluorescent dye phosphoramidite labelling of oligonucleotides.
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Journal: Nucleic acids symposium series (1992): 99-100

6-FAM亚磷酰胺单体 5’-荧光素氨基磷酸酯

	货号	6016	存储条件	在零下15度以下保存, 避免光照
	规格	100 µmoles	价格	1668
	Ex (nm)	493	Em (nm)	517
	分子量	843.94	溶剂	MeCN
	产品详细介绍

简要概述

产品基本信息

货号：6016

产品名称：6-FAM亚磷酰胺单体 5’-荧光素氨基磷酸酯

CAS：204697-37-0

规格：100 µmoles

储存条件：-15℃避光防潮

保质期：6个月

产品物理化学光谱特性

分子量：843.94

溶剂：MeCN

激发波长（nm）：494

发射波长（nm）：522

产品介绍

6-FAM亚磷酰胺单体 5’-荧光素氨基磷酸酯是美国AAT Bioquest生产的荧光染料。有几种用荧光素标记寡核苷酸的方法。根据光谱要求，标签的选择进一步多样化。5′-荧光素-CE亚磷酰胺，衍生自单一异构体6-羧基荧光素，5′-六氯荧光素-CE亚磷酰胺（HEX）和5′-四氯荧光素-CE亚磷酰胺（TET）均可用于有效标记寡核苷酸。 ‘-结束。用荧光素标记寡核苷酸的3’末端可以使用3’-荧光素CPG实现。当使用任何这些载体时，用氢氧化铵进行标准裂解和去保护释放荧光素标记的寡核苷酸。金畔生物是AAT Bioquest的中国代理商，为您提供最优质的6-FAM亚磷酰胺单体 5’-荧光素氨基磷酸酯。 

点击查看光谱

参考文献

Sequence-specific, self-reporting hairpin inversion probes
Authors: Browne KA.
Journal: J Am Chem Soc (2005): 1989

Modified oligonucleotides containing lithocholic acid in their backbones: their enhanced cellular uptake and their mimicking of hairpin structures
Authors: Kim SJ, Bang EK, Kwon HJ, Shim JS, Kim BH.
Journal: Chembiochem (2004): 1517

Synthesis of HyBeacons and dual-labelled probes containing 2′-fluorescent groups for use in genetic analysis
Authors: Dobson N, McDowell DG, French DJ, Brown LJ, Mellor JM, Brown T.
Journal: Chem Commun (Camb) (2003): 1234

Reduced aggregation and improved specificity of G-rich oligodeoxyribonucleotides containing pyrazolo[3,4-d]pyrimidine guanine bases
Authors: Kutyavin IV, Lokhov SG, Afonina IA, Dempcy R, Gall AA, Gorn VV, Lukhtanov E, Metcalf M, Mills A, Reed MW, Sanders S, Shishkina I, Vermeulen NM.
Journal: Nucleic Acids Res (2002): 4952

Diels–Alder bioconjugation of diene-modified oligonucleotides
Authors: Hill KW, Taunton-Rigby J, Carter JD, Kropp E, Vagle K, Pieken W, McGee DP, Husar GM, Leuck M, Anziano DJ, Sebesta DP.
Journal: J Org Chem (2001): 5352

Recovery of an oligonucleotide using silver ions immobilized onto paramagnetic particles
Authors: Ramirez-Vick JE, Garcia AA, Lee J.
Journal: Prep Biochem Biotechnol (1998): 243

Simultaneous detection of several oligonucleotides by time-resolved fluorometry: the use of a mixture of categorized microparticles in a sandwich type mixed-phase hybridization assay
Authors: Hakala H, Virta P, Salo H, Lonnberg H.
Journal: Nucleic Acids Res (1998): 5581

Synthesis and characterization of a new 5-thiol-protected deoxyuridine phosphoramidite for site-specific modification of DNA
Authors: Meyer KL, Hanna MM.
Journal: Bioconjug Chem (1996): 401

Characterization of endonucleolytic activity of HIV-1 integrase using a fluorogenic substrate
Authors: Lee SP, Censullo ML, Kim HG, Knutson JR, Han MK.
Journal: Anal Biochem (1995): 295

Synthesis and characterisation of fluorescent oligonucleotides. Effect of internal labelling on protein recognition
Authors: Hagmar P, Bailey M, Tong G, Haralambidis J, Sawyer WH, Davidson BE.
Journal: Biochim Biophys Acta (1995): 259

相关产品

6-FAM 6-羧基荧光素炔烃

	货号	134	存储条件	在零下15度以下保存, 避免光照
	规格	10 mg	价格	1944
	Ex (nm)	493	Em (nm)	517
	分子量	413.38	溶剂	DMSO
	产品详细介绍

简要概述

产品基本信息

货号：134

产品名称：6-FAM 6-羧基荧光素炔烃

规格：10mg

储存条件：-15℃避光防潮

保质期：12个月

产品物理化学光谱特性

分子量：413.38

溶剂：DMSO

激发波长（nm）：494

发射波长（nm）：518

产品介绍

6-FAM 6-羧基荧光素炔烃是美国AAT Bioquest生产的荧光素，6-FAM炔烃是通过与叠氮基团反应来开发荧光素生物共轭物的良好构件，它被广泛用于标记寡核苷酸。 6-FAM是羧基荧光素的纯化单一异构体。金畔生物是AAT Bioquest的中国代理商，为您提供最优质的6-FAM 6-羧基荧光素炔烃。 

点击查看光谱

参考文献

Multicellular Vascularized Engineered Tissues through User-Programmable Biomaterial Photodegradation
Authors: Christopher K Arakawa, Barry A Badeau, Ying Zheng, Cole A DeForest
Journal: Advanced Materials (2017)

Green tea inhibited the elimination of nephro-cardiovascular toxins and deteriorated the renal function in rats with renal failure
Authors: Yu-Hsuan Peng, Douglas H Sweet, Shiuan-Pey Lin, Chung-Ping Yu, Pei-Dawn Lee Chao, Yu-Chi Hou
Journal: Scientific reports (2015)

Advances in Quantitative FRET-Based Methods for Studying Nucleic Acids
Authors: Søren Preus, L Marcus Wilhelmsson
Journal: ChemBioChem (2012): 1990–2001

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