C-藻蓝蛋白C-PC CAS 11016-15-2 货号2553-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。

C-藻蓝蛋白C-PC CAS 11016-15-2

C-藻蓝蛋白C-PC CAS 11016-15-2

C-藻蓝蛋白C-PC CAS 11016-15-2    货号2553 货号 2553 存储条件 在2-8度冷藏保存, 避免光照
规格 1 mg 价格 1272
Ex (nm) 616 Em (nm) 647
分子量 ~264,000 溶剂 Water
产品详细介绍

简要概述

产品基本信息

CAS:11016-15-2

分子量:~264,000

Ex(nm):616

Ex(nm):647

荧光量子产率(QY):0.81

 

产品介绍

藻胆蛋白[包括B-藻红蛋白(B-PE),R-藻红蛋白(R-PE)和别藻蓝蛋白(APC)]是用于生物学检测的超灵敏荧光染料。 它们比传统的有机荧光团灵敏度高100倍。 即使在诸如流式细胞术和免疫测定的实际应用中,藻胆蛋白缀合的抗体的灵敏度通常远大于相应的基于有机分子的缀合物的灵敏度。 Phycobiliproteins是最亮的荧光标签,具有多个位点,可与许多生物和合成材料形成稳定的结合。

 

  • 藻红蛋白(B-PE)具有三个吸收带,在545nm处具有最大吸收。 B-PE的亚基结构类似于R-PE,但亚基的发色团含量不同,导致吸收峰的相对强度不同:α和β亚基仅含有PEB,而γ亚基含有PEB和PUB。 B-PE存在于蓝细菌和红藻中。 B-PE的强烈粉红色和橙色荧光几乎与肉眼无法区分的R-PE。别藻蓝蛋白在主要的藻胆蛋白中是最不稳定的,在低浓度下易于解离,包括进行某些测定的浓度。 出于这个原因,许多研究人员更喜欢使用在α和β亚基之间化学交联的CL-APC,并且比APC更稳定。

 

  • 藻红蛋白(R-PE)从红藻中分离。其主要吸收峰位于565nm,第二峰位于496nm和545nm。 次生峰的相对突出性在来自不同物种的R-PE之间显着不同。R-PE具有三种类型的亚基:α(~20,000道尔顿),β(~20,000道尔顿)和γ(~30,000道尔顿)。已发现完整R-PE的分子量为约240,000道尔顿,并且已确定(αβ)6γ的亚基结构。R-PE的α亚基仅含有藻红蛋白(PEB)发色团,而β和γ亚基含有PEB和藻蓝蛋白(PUB)。来自不同物种的R-PE的吸收光谱的可变性反映了亚基的PEB / PUB比率的差异。R-PE和密切相关的B-PE是最强荧光的藻胆蛋白,其量子效率可能超过90%,并且在任何中等浓度的溶液中,其橙色荧光很容易被肉眼看到。

 

C-藻蓝蛋白(C-PC)在许多蓝细菌中作为主要的藻胆蛋白发生,在一些红藻中作为次生的藻胆蛋白发生。该颜料在615和620nm之间具有单个可见吸收最大值,在~650nm处具有最大荧光发射。其分子量在70,000至110,000道尔顿之间。颜料由两个亚基α和β组成,它们以相同的数量出现,但构成分子的α和β对的确切数目可能因物种而异。α和β亚基都仅含有PCB发色团。 除了直接吸收光之外,这种强烈的蓝色颜料通过荧光能量转移接受来自藻红蛋白的量子,其中存在PE的生物体。C-PC的红色荧光转移到别藻蓝蛋白。AAT Bioquest研发的RPE、APC试剂及试剂盒被国内外科研人员灵活运用,达到不俗的科研成果。

 

参考文献

Chromophore attachment to phycobiliprotein beta-subunits: phycocyanobilin:cysteine-beta84 phycobiliprotein lyase activity of CpeS-like protein from Anabaena Sp. PCC7120
Authors: Zhao KH, Su P, Li J, Tu JM, Zhou M, Bubenzer C, Scheer H.
Journal: J Biol Chem (2006): 8573

Excitation energy transfer from phycobiliprotein to chlorophyll d in intact cells of Acaryochloris marina studied by time- and wavelength-resolved fluorescence spectroscopy
Authors: Petrasek Z, Schmitt FJ, Theiss C, Huyer J, Chen M, Larkum A, Eichler HJ, Kemnitz K, Eckert HJ.
Journal: Photochem Photobiol Sci (2005): 1016

Single-molecule spectroscopy selectively probes donor and acceptor chromophores in the phycobiliprotein allophycocyanin
Authors: Loos D, Cotlet M, De Schryver F, Habuchi S, Hofkens J.
Journal: Biophys J (2004): 2598

Evaluation of Tolypothrix germplasm for phycobiliprotein content
Authors: Prasanna R, Prasanna BM, Mohammadi SA, Singh PK.
Journal: Folia Microbiol (Praha) (2003): 59

Isolation and characterisation of phycobiliprotein rich mutant of cyanobacterium Synechocystis sp
Authors: Prasanna R, Dhar DW, Dominic TK, Tiwari ON, Singh PK.
Journal: Acta Biol Hung (2003): 113

Co-ordinated expression of phycobiliprotein operons in the chromatically adapting cyanobacterium Calothrix PCC 7601: a role for RcaD and RcaG
Authors: Noubir S, Luque I, Ochoa de Alda JA, Perewoska I, Tandeau de Marsac N, Cobley JG, Houmard J.
Journal: Mol Microbiol (2002): 749

Phycobiliprotein genes of the marine photosynthetic prokaryote Prochlorococcus: evidence for rapid evolution of genetic heterogeneity
Authors: Ting CS, Rocap G, King J, Chisholm SW.
Journal: Microbiology (2001): 3171

Novel activity of a phycobiliprotein lyase: both the attachment of phycocyanobilin and the isomerization to phycoviolobilin are catalyzed by the proteins PecE and PecF encoded by the phycoerythrocyanin operon
Authors: Zhao KH, Deng MG, Zheng M, Zhou M, Parbel A, Storf M, Meyer M, Strohmann B, Scheer H.
Journal: FEBS Lett (2000): 9

Phycobiliprotein-Fab conjugates as probes for single particle fluorescence imaging
Authors: Triantafilou K, Triantafilou M, Wilson KM.
Journal: Cytometry (2000): 226

[Phycobiliprotein and fluorescence immunological assay]
Authors: Wu P.
Journal: Sheng Li Ke Xue Jin Zhan (2000): 82

说明书
C-藻蓝蛋白C-PC CAS 11016-15-2.pdf

DBCO 琥珀酰亚胺酯 CAS 1353016-71-3 货号4529-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。

DBCO 琥珀酰亚胺酯 CAS 1353016-71-3

DBCO 琥珀酰亚胺酯 CAS 1353016-71-3

DBCO 琥珀酰亚胺酯 CAS 1353016-71-3    货号4529 货号 4529 存储条件 在零下15度以下保存, 避免光照
规格 10 mg 价格 1272
Ex (nm) Em (nm)
分子量 402.41 溶剂 DMSO
产品详细介绍

简要概述

产品基本信息

货号:4529

产品名称:DBCO 琥珀酰亚胺酯

CAS:1353016-71-3

规格:10mg

储存条件:-15℃避光防潮

保质期:24个月

 

产品物理化学光谱特性

分子量:402.41

外观:固体

溶剂:DMSO

 

产品介绍

无铜点击化学反应,即菌株促进的叠氮炔环加成(SPAAC)是一种生物正交反应,利用一对试剂,即环辛基和叠氮化合物,它们彼此有效地反应,同时对羟基、氨基和硫醇等天然官能团保持惰性。SPAAC能够通过形成稳定的三唑,在水和其他复杂的化学环境中标记各种各样的生物分子,而无需任何辅助试剂。在众多已知的环辛基化合物中,二苯并环辛基化合物(DBCO)是一类具有相当快的动力学和良好的水缓冲稳定性的试剂。在生理温度和pH范围内,DBCO基团不会与许多生物分子中天然存在的巯基、氨基或羟基发生反应。DBCO-琥珀酰亚胺酯是一种常用的通过氨基修饰将DBCO基团引入生物分子的试剂。金畔生物是AAT Bioquest的中国代理商,为您提供最优质的DBCO 琥珀酰亚胺酯。 

说明书
DBCO 琥珀酰亚胺酯 CAS 1353016-71-3.pdf

Cy5 酸 CAS 146368-11-8 货号150-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。

Cy5 酸 CAS 146368-11-8

Cy5 酸 CAS 146368-11-8

Cy5 酸 CAS 146368-11-8    货号150 货号 150 存储条件 在零下15度以下保存, 避免光照
规格 5 mg 价格 2604
Ex (nm) 651 Em (nm) 670
分子量 770.83 溶剂 DMSO
产品详细介绍

简要概述

产品基本信息

货号:150

产品名称:Cy5,酸

CAS:146368-11-8

规格:5mg

储存条件:-15℃避光防潮

保质期:12个月

 

产品物理化学光谱特性

分子量:770.83

外观:蓝色固体

溶剂:DMSO

激发波长(nm):649

发射波长(nm):665

 

产品介绍

Cy5,酸(Cy5)常用于荧光成像和其他基于荧光的生化分析。它们广泛用于标记肽,蛋白质和寡核苷酸等。Cy5染料是最常见的红色荧光团中的一种。这些多功能荧光团用于生物相关pH的各种应用时可以耐受3-10范围的pH。该染料还具有DMSO耐受性和光稳定性,能够在不损失性能的情况下从储存转移至分析。它的水溶性消除了在测定缓冲液中对有机溶剂的需要。我们的Cy5荧光染料经过全面QC测试,可确保高水平的发色团和活性染料含量。单活性染料适用于蛋白质和寡核苷酸的靶向-精确标记,双活性染料更适用于一般标记。建议使用NHS酯染料标记胺基,建议使用马来酰亚胺染料标记硫醇基团。

点击查看光谱

 

参考文献

Cube-shaped theranostic paclitaxel prodrug nanocrystals with surface functionalization of SPC and MPEG-DSPE for imaging and chemotherapy
Authors: Fuqiang Guo, Jiajia Shang, Hai Zhao, Kangrong Lai, Yang Li, Zhongxiong Fan, Zhenqing Hou, Guanghao Su
Journal: Colloids and Surfaces B: Biointerfaces (2017)

Light/magnetic hyperthermia triggered drug released from multi-functional thermo-sensitive magnetoliposomes for precise cancer synergetic theranostics
Authors: Yuxin Guo, Yang Zhang, Jinyuan Ma, Qi Li, Yang Li, Xinyi Zhou, Dan Zhao, Hua Song, Qing Chen, Xuan Zhu
Journal: Journal of Controlled Release (2017)

Thermo-sensitive hydrogel PLGA-PEG-PLGA as a vaccine delivery system for intramuscular immunization
Authors: Xiaoyan Wang, Yu Zhang, Wei Xue, Hong Wang, Xiaozhong Qiu, Zonghua Liu
Journal: Journal of Biomaterials Applications (2017): 923–932

Affinity-Controlled Protein Encapsulation into Sub-30 nm Telodendrimer Nanocarriers by Multivalent and Synergistic Interactions
Authors: Xu Wang, Changying Shi, Li Zhang, Alexa Bodman, Dandan Guo, Lili Wang, Walter A Hall, Stephan Wilkens, Juntao Luo
Journal: Biomaterials (2016)

Carboxymethyl Dextran-Stabilized Polyethylenimine-Poly (epsilon-caprolactone) Nanoparticles-Mediated Modulation of MicroRNA-34a Expression via Small-Molecule Modulator for Hepatocellular Carcinoma Therapy
Authors: Xiongwei Deng, Zhaoxia Yin, Zhixiang Zhou, Yihui Wang, Fang Zhang, Qin Hu, Yishu Yang, Jianqing Lu, Yan Wu, Wang Sheng
Journal: ACS applied materials & interfaces (2016): 17068–17079

Click-electron microscopy for imaging metabolically tagged nonprotein biomolecules
Authors: John T Ngo, Stephen R Adams, Thomas J Deerinck, Daniela Boassa, Frances Rodriguez-Rivera, Sakina F Palida, Carolyn R Bertozzi, Mark H Ellisman, Roger Y Tsien
Journal: Nat Chem Biol (2016): 459–465

Design, synthesis and evaluation of VEGF-siRNA/CRS as a novel vector for gene delivery
Authors: Wen Zhao, Yifan Zhang, Xueyun Jiang, Chunying Cui
Journal: Drug Design, Development and Therapy (2016): 3851

Molecular Basis and Consequences of the Cytochrome c-tRNA Interaction
Authors: Cuiping Liu, Aaron J Stonestrom, Thomas Christian, Jeongsik Yong, Ryuichi Takase, Ya-Ming Hou, Xiaolu Yang
Journal: Journal of Biological Chemistry (2016): 10426–10436

Determination of the active transport of fucoidan derived from okinawa mozuku across the human intestinal caco-2 cells as assessed by size-exclusion chromatography
Authors: Takeaki Nagamine, Kou Hayakawa, Kyoumi Nakazato, Masahiko Iha
Journal: Journal of Chromatography B (2015): 187–193

Multiplexed single-cell in situ RNA analysis by reiterative hybridization
Authors: Lu Xiao, Jia Guo
Journal: Analytical Methods (2015): 7290–7295

说明书
Cy5 酸 CAS 146368-11-8.pdf

Cy7 NHS酯 CAS 477908-53-5 货号161-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。

Cy7 NHS酯 CAS 477908-53-5

Cy7 NHS酯 CAS 477908-53-5

Cy7 NHS酯 CAS 477908-53-5    货号161 货号 161 存储条件 在零下15度以下保存, 避免光照
规格 1 mg 价格 1272
Ex (nm) 756 Em (nm) 779
分子量 881.11 溶剂 DMSO
产品详细介绍

简要概述

产品基本信息

货号:161

产品名称:Cy7,NHS酯

CAS:477908-53-5

规格:1mg

储存条件:-15℃干燥

保质期:12个月

 

产品物理化学光谱特性

分子量:881.11

外观:深蓝色固体

溶剂:DMSO

激发波长(nm):749

发射波长(nm):776

 

产品介绍

Cy7,NHS酯(Cy7)已用于标记生物分子,用于荧光成像和其他基于荧光的生化分析。它们广泛用于标记肽,蛋白质和寡核苷酸等。Cy7染料缀合物是体内成像应用中最常用的近红外红色荧光团的一种。Cy7®NHS酯易与氨基反应。

点击查看光谱

点击查看实验方案

 

参考文献

Hexapeptide-conjugated calcitonin for targeted therapy of osteoporosis
Authors: Yanpeng Liu, Peng Yu, Xu Peng, Qin Huang, Mingming Ding, Yantao Chen, Ruitao Jin, Jing Xie, Changsheng Zhao, Jianshu Li
Journal: Journal of Controlled Release (2019)

Cube-shaped theranostic paclitaxel prodrug nanocrystals with surface functionalization of SPC and MPEG-DSPE for imaging and chemotherapy
Authors: Fuqiang Guo, Jiajia Shang, Hai Zhao, Kangrong Lai, Yang Li, Zhongxiong Fan, Zhenqing Hou, Guanghao Su
Journal: Colloids and Surfaces B: Biointerfaces (2017)

Light/magnetic hyperthermia triggered drug released from multi-functional thermo-sensitive magnetoliposomes for precise cancer synergetic theranostics
Authors: Yuxin Guo, Yang Zhang, Jinyuan Ma, Qi Li, Yang Li, Xinyi Zhou, Dan Zhao, Hua Song, Qing Chen, Xuan Zhu
Journal: Journal of Controlled Release (2017)

Thermo-sensitive hydrogel PLGA-PEG-PLGA as a vaccine delivery system for intramuscular immunization
Authors: Xiaoyan Wang, Yu Zhang, Wei Xue, Hong Wang, Xiaozhong Qiu, Zonghua Liu
Journal: Journal of Biomaterials Applications (2017): 923–932

Affinity-Controlled Protein Encapsulation into Sub-30 nm Telodendrimer Nanocarriers by Multivalent and Synergistic Interactions
Authors: Xu Wang, Changying Shi, Li Zhang, Alexa Bodman, Dandan Guo, Lili Wang, Walter A Hall, Stephan Wilkens, Juntao Luo
Journal: Biomaterials (2016)

Carboxymethyl Dextran-Stabilized Polyethylenimine-Poly (epsilon-caprolactone) Nanoparticles-Mediated Modulation of MicroRNA-34a Expression via Small-Molecule Modulator for Hepatocellular Carcinoma Therapy
Authors: Xiongwei Deng, Zhaoxia Yin, Zhixiang Zhou, Yihui Wang, Fang Zhang, Qin Hu, Yishu Yang, Jianqing Lu, Yan Wu, Wang Sheng
Journal: ACS applied materials & interfaces (2016): 17068–17079

Click-electron microscopy for imaging metabolically tagged nonprotein biomolecules
Authors: John T Ngo, Stephen R Adams, Thomas J Deerinck, Daniela Boassa, Frances Rodriguez-Rivera, Sakina F Palida, Carolyn R Bertozzi, Mark H Ellisman, Roger Y Tsien
Journal: Nat Chem Biol (2016): 459–465

Design, synthesis and evaluation of VEGF-siRNA/CRS as a novel vector for gene delivery
Authors: Wen Zhao, Yifan Zhang, Xueyun Jiang, Chunying Cui
Journal: Drug Design, Development and Therapy (2016): 3851

Molecular Basis and Consequences of the Cytochrome c-tRNA Interaction
Authors: Cuiping Liu, Aaron J Stonestrom, Thomas Christian, Jeongsik Yong, Ryuichi Takase, Ya-Ming Hou, Xiaolu Yang
Journal: Journal of Biological Chemistry (2016): 10426–10436

Determination of the active transport of fucoidan derived from okinawa mozuku across the human intestinal caco-2 cells as assessed by size-exclusion chromatography
Authors: Takeaki Nagamine, Kou Hayakawa, Kyoumi Nakazato, Masahiko Iha
Journal: Journal of Chromatography B (2015): 187–193

 

相关产品

产品名称 货号
Cy7 双NHS酯 Cat#170
Cy5 NHS酯 Cat#151
Cy3 NHS酯 Cat#141

说明书
Cy7 NHS酯 CAS 477908-53-5.pdf

5-DTAF CAS 51306-35-5 货号200-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。

5-DTAF CAS 51306-35-5

5-DTAF CAS 51306-35-5

5-DTAF CAS 51306-35-5    货号200 货号 200 存储条件 在零下15度以下保存, 避免光照
规格 25 mg 价格 1272
Ex (nm) 498 Em (nm) 517
分子量 495.28 溶剂 DMSO
产品详细介绍

简要概述

产品基本信息

货号:200

产品名称:5-DTAF [5-(4,6-二氯三嗪基)氨基荧光素]

CAS:51306-35-5

规格:25mg

储存条件:-15℃避光防潮

保质期:24个月

 

产品物理化学光谱特性

分子量:495.28

外观:黄色固体

溶剂:DMSO

激发波长(nm):497

发射波长(nm):516

 

产品介绍

荧光素二氯三嗪(5-DTAF)的5-异构体与蛋白质具有高度反应性。 与其他反应性荧光素不同,5-DTAF不仅与氨基反应,而且还与硫醇基团反应,甚至在pH大于9的水溶液中直接与羟基如多糖和其他醇反应。由于其高反应性,5- DTAF也用于标记碳水化合物。

点击查看光谱

 

参考文献

Fluorescently labeled collagen binding proteins allow specific visualization of collagen in tissues and live cell culture
Authors: Krahn KN, Bouten CV, van Tuijl S, van Zandvoort MA, Merkx M.
Journal: Anal Biochem (2006): 177

Mitomycin C alters corneal stromal wound healing and corneal haze in rabbits after argon-fluoride excimer laser photorefractive keratectomy
Authors: Lai YH, Wang HZ, Lin CP, Chang SJ.
Journal: J Ocul Pharmacol Ther (2004): 129

Fluorescent cellulose microfibrils as substrate for the detection of cellulase activity
Authors: Helbert W, Chanzy H, Husum TL, Schulein M, Ernst S.
Journal: Biomacromolecules (2003): 481

Microbial activity in soils following steam treatment
Authors: Richardson RE, James CA, Bhupathiraju VK, Alvarez-Cohen L.
Journal: Biodegradation (2002): 285

Effect of collagenase inhibitors on corneal haze after PRK
Authors: Corbett MC, O’Brart DP, Patmore AL, Marshall J.
Journal: Exp Eye Res (2001): 253

Glutamic acid decarboxylase-like immunoreactive axon terminals in synaptic contact with mesencephalic trigeminal nucleus neurons in the rat
Authors: Chen P, Li J, Mizuno N.
Journal: Neurosci Lett (2001): 167

Redistribution of plasma-membrane surface molecules during formation of the Leishmania amazonensis-containing parasitophorous vacuole
Authors: Henriques C, de Souza W.
Journal: Parasitol Res (2000): 215

Application of a tetrazolium dye as an indicator of viability in anaerobic bacteria
Authors: Bhupathiraju VK, Hernandez M, Landfear D, Alvarez-Cohen L.
Journal: J Microbiol Methods (1999): 231

Leakage responses to L-NAME differ with the fluorescent dye used to label albumin
Authors: Rumbaut RE, Harris NR, Sial AJ, Huxley VH, Granger DN.
Journal: Am J Physiol (1999): H333

Collagen fiber sliding during ligament growth and contracture
Authors: Wood ML, Lester GE, Dahners LE.
Journal: J Orthop Res (1998): 438

说明书
5-DTAF CAS 51306-35-5.pdf