mFluor™ Violet 540 Biotin Conjugate 货号3114-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。

mFluor™ Violet 540 Biotin Conjugate

mFluor™ Violet 540 Biotin Conjugate

货号 3114 存储条件 在零下15度以下保存, 避免光照
规格 1 mg 价格 2604
Ex (nm) 402 Em (nm) 535
分子量 1504.80 溶剂 DMSO
产品详细介绍

简要概述

说明书
mFluor™ Violet 540 Biotin Conjugate.pdf

ReadiUse™ Preactivated PE-Cy5.5 Maleimide 货号2718-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。

ReadiUse™ Preactivated PE-Cy5.5 Maleimide

ReadiUse™ Preactivated PE-Cy5.5 Maleimide

货号 2718 存储条件 在2-8度冷藏保存, 避免光照
规格 1 mg 价格 4740
Ex (nm) 566 Em (nm) 671
分子量 N/A 溶剂 Water
产品详细介绍

简要概述

说明书
ReadiUse™ Preactivated PE-Cy5.5 Maleimide.pdf

ReadiLink™xtra Rapid iFluor™488抗体标记试剂盒(标记50ug抗体) 货号1955-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。

ReadiLink™xtra Rapid iFluor™488抗体标记试剂盒(标记50ug抗体)

ReadiLink™xtra Rapid iFluor™488抗体标记试剂盒(标记50ug抗体)

ReadiLink™xtra Rapid iFluor™488抗体标记试剂盒(标记50ug抗体)    货号1955 货号 1955 存储条件 在零下15度以下保存, 避免光照
规格 2 Labelings 价格 2472
Ex (nm) 491 Em (nm) 516
分子量 溶剂
产品详细介绍

简要概述

ReadiLink™xtra快速抗体标记试剂是AAT 研发,由金畔代理销售的iFluor488标记的抗体标记试剂盒。ReadiLink™xtra快速抗体标记试剂盒基本上只需要2个简单的混合步骤,而无需纯化。ReadiLink™试剂盒中使用的预活化iFluor™488非常稳定,对抗体表现出良好的反应性和选择性。该试剂盒具有用于标记〜2×50 ug抗体的所有基本成分。试剂盒中提供的两个预活化iFluor™488染料小瓶中的每一个均经过优化,可标记约50 µg抗体。ReadiLink™xtra iFluor™488快速抗体标记试剂盒提供了一种方便而强大的方法,可以使用绿色的iFluor™488荧光团标记单克隆和多克隆抗体。金畔生物是AAT Bioquest的中国代理商,为您提供最优质的抗体标记试剂盒。 

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产品说明书

实验方案

工作溶液配制

蛋白质工作溶液(A)

为了标记50 µg蛋白质(假设目标蛋白质浓度为1 mg / mL),请将5 µL(反应总体积的10%)反应缓冲液(组分B)与50 µL目标蛋白质溶液混合。
注意:如果蛋白质浓度不同,请相应地调整蛋白质体积,以使〜50 µg蛋白质可用于标记反应。
注意:要标记100 µg蛋白质(假设目标蛋白质浓度为1 mg / mL),请将10 µL(反应总体积的10%)反应缓冲液(组分B)与100 µL目标蛋白质溶液混合。
注意:蛋白质应溶于1X磷酸盐缓冲盐水(PBS),pH 7.2-7.4;如果蛋白质溶解在甘氨酸缓冲液中,则必须针对1X PBS(pH 7.2-7.4)进行透析,或使用10 kDa的Amicon Ultra-0.5,Ultracel-10膜去除游离的胺或铵盐(例如硫酸铵和乙酸铵)被广泛用于蛋白质沉淀。
注意:为获得最佳标记效率,建议最终蛋白质浓度范围为1-2 mg / mL,结合效率明显降低,低于1 mg / mL。 

 

操作步骤

运行缀合反应
  1. 将蛋白质工作溶液(溶液A)添加到一个小瓶的标记染料(组分A)中,并通过将小瓶涡旋几秒钟将它们充分混合。
    注意:如果要标记100 µg的蛋白质,请使用两个小瓶(组分A),将100 µg的蛋白质分成2 x 50 µg的蛋白质,并使每个50 µg的蛋白质与一小瓶的标记染料反应。然后合并两个小瓶,用于下一步。
  2. 将缀合反应混合物在室温下放置30-60分钟。
    注意:如果需要,可以旋转或摇动缀合反应混合物更长的时间。 

 

停止缀合反应
  1. 将5 µL(对于50 µg蛋白质)或10 µL(对于100 µg蛋白质)添加到缀合反应混合物中,占TQ™染色猝灭缓冲液(组分C)总反应体积的10%;混合均匀。
  2. 在室温下孵育10分钟。标记的蛋白(抗体)可以使用了。 

 

蛋白质缀合物的储存

蛋白质缀合物应在载体蛋白(例如0.1%牛血清白蛋白)存在下以> 0.5 mg / mL的浓度存储。为了更长的存储时间,可以将蛋白质缀合物冻干或分成单份使用,并存储在≤–20°C下。

 

图示

ReadiLink™xtra Rapid iFluor™488抗体标记试剂盒(标记50ug抗体)    货号1955

图1. HeLa细胞中微管蛋白的免疫荧光染色。HeLa细胞用4%PFA固定,用0.1%Triton X-100透化并封闭。然后将细胞与小鼠抗微管蛋白抗体一起孵育,并用ReadiLink™xtra Rapid iFluor™488抗体标记试剂盒(#1955)标记的山羊抗小鼠IgG染色。

  

参考文献

Identification of a Small Probe That Can Be Conjugated to Proteins by Proximity Labeling.
Authors: Sun, Weiping and Huo, Yinbo and Mei, Yuxuan and Zhou, Qingtong and Zhao, Suwen and Zhuang, Min
Journal: ACS chemical biology (2020): 39-43

Paper-based nuclease protection assay with on-chip sample pretreatment for point-of-need nucleic acid detection.
Authors: Noviana, Eka and Jain, Sidhartha and Hofstetter, Josephine and Geiss, Brian J and Dandy, David S and Henry, Charles S
Journal: Analytical and bioanalytical chemistry (2020): 3051-3061

A neuraminidase potency assay for quantitative assessment of neuraminidase in influenza vaccines.
Authors: Byrne-Nash, Rose T and Gillis, Jacob H and Miller, David F and Bueter, Katie M and Kuck, Laura R and Rowlen, Kathy L
Journal: NPJ vaccines (2019): 3

Author Correction: A dynamic three-step mechanism drives the HIV-1 pre-fusion reaction.
Authors: Iliopoulou, Maro and Nolan, Rory and Alvarez, Luis and Watanabe, Yasunori and Coomer, Charles A and Jakobsdottir, G Maria and Bowden, Thomas A and Padilla-Parra, Sergi
Journal: Nature structural & molecular biology (2019): 526

Site-Specific Fluorescent Labeling of Antibodies and Diabodies Using SpyTag/SpyCatcher System for In Vivo Optical Imaging.
Authors: Alam, Md Kausar and El-Sayed, Ayman and Barreto, Kris and Bernhard, Wendy and Fonge, Humphrey and Geyer, C Ronald
Journal: Molecular imaging and biology (2019): 54-66

Highly efficient electrochemical sensing platform for sensitive detection DNA methylation, and methyltransferase activity based on Ag NPs decorated carbon nanocubes.
Authors: Gao, Fenglei and Fan, Taotao and Ou, Shanshan and Wu, Jing and Zhang, Xing and Luo, Jianjun and Li, Na and Yao, Yao and Mou, Yingfeng and Liao, Xianjiu and Geng, Deqin
Journal: Biosensors & bioelectronics (2018): 201-208

Improved performance of lateral flow immunoassays for alpha-fetoprotein and vanillin by using silica shell-stabilized gold nanoparticles.
Authors: Lu, Xuewen and Mei, Ting and Guo, Qi and Zhou, Wenjing and Li, Xiaomei and Chen, Jitao and Zhou, Xinke and Sun, Ning and Fang, Zhiyuan
Journal: Mikrochimica acta (2018): 2

Intracellular in situ labeling of TiO2 nanoparticles for fluorescence microscopy detection.
Authors: Brown, Koshonna and Thurn, Ted and Xin, Lun and Liu, William and Bazak, Remon and Chen, Si and Lai, Barry and Vogt, Stefan and Jacobsen, Chris and Paunesku, Tatjana and Woloschak, Gayle E
Journal: Nano research (2018): 464-476

Multiplex Immunoassay Profiling of Serum in Psychiatric Disorders.
Authors: Stephen, Laurie and Schwarz, Emanuel and Guest, Paul C
Journal: Advances in experimental medicine and biology (2017): 149-156

Multiplex Immunoassay Profiling.
Authors: Stephen, Laurie
Journal: Methods in molecular biology (Clifton, N.J.) (2017): 169-176

说明书
ReadiLink™xtra Rapid iFluor™488抗体标记试剂盒(标记50ug抗体).pdf

Bodi Fluor™576/589NHS酯(相当于BODIPY™576/589) CAS 201998-61-0 货号661-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。

Bodi Fluor™576/589NHS酯(相当于BODIPY™576/589) CAS 201998-61-0

Bodi Fluor™576/589NHS酯(相当于BODIPY™576/589) CAS 201998-61-0

Bodi Fluor™576/589NHS酯(相当于BODIPY™576/589) CAS 201998-61-0    货号661 货号 661 存储条件 在零下15度以下保存, 避免光照
规格 5 mg 价格 3732
Ex (nm) 573 Em (nm) 592
分子量 426.19 溶剂 DMSO
产品详细介绍

简要概述

产品基本信息

货号:661

产品名称:Bodi Fluor™576/589NHS酯(相当于BODIPY™576/589)

CAS:201998-61-0

规格:5 mg

储存条件:-15℃避光防潮

保质期:12个月

 

产品物理化学光谱特性

分子量:426.19

外观:固体

溶剂:DMSO

 

产品介绍

Bodi Fluor™576/589在化学上与BODIPY™576/589染料相同。它是一种具有高细胞渗透性的亮红色荧光染料。它具有高消光系数和荧光量子产率。它的荧光对溶剂极性和pH变化相对不敏感。与高度水溶性的荧光团(例如iFluor™和AlexaFluor®488染料)相反,Bodi Fluor染料具有独特的疏水性,非常适合对脂质,膜和其他亲脂性化合物进行染色。Bodi Fluor 576/589染料具有相对较长的激发态寿命(通常为5纳秒或更长时间),可用于基于荧光偏振的测定法,而较大的两光子横截面可用于多光子激发。

点击查看光谱

 

参考文献

Absorption properties of a BODIPY-curved graphene nanoflake system: A theoretical investigation.
Authors: Petrushenko, I K and Petrushenko, K B
Journal: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy (2020): 117465

Amphiphilic BODIPY dye aggregates in polymeric micelles for wavelength-dependent photo-induced cancer therapy.
Authors: Yan, Ziling and Wang, Mengya and Shi, Mengke and He, Yang and Zhang, Yi and Qiu, Shihong and Yang, Hong and Chen, Huabing and He, Hui and Guo, Zhengqing
Journal: Journal of materials chemistry. B (2020)

Exploring the Relationship between BODIPY Structure and Spectroscopic Properties to Design Fluorophores for Bioimaging.
Authors: Donnelly, Joanna L and Offenbartl-Stiegert, Daniel and Marín-Beloqui, José M and Rizzello, Loris and Battaglia, Guiseppe and Clarke, Tracey M and Howorka, Stefan and Wilden, Jonathan D
Journal: Chemistry (Weinheim an der Bergstrasse, Germany) (2020): 863-872

Living Supramolecular Polymerization of an Aza-BODIPY Dye Controlled by a Hydrogen-Bond-Accepting Triazole Unit Introduced by Click Chemistry.
Authors: Wang, Houchen and Zhang, Yongjie and Chen, Yuanfang and Pan, Hongfei and Ren, Xiangkui and Chen, Zhijian
Journal: Angewandte Chemie (International ed. in English) (2020): 5185-5192

Microalgae Lipid Staining with Fluorescent BODIPY Dye.
Authors: Koreivienė, Judita
Journal: Methods in molecular biology (Clifton, N.J.) (2020): 47-53

Targeting EGFR Overexpression at the Surface of Colorectal Cancer Cells by Exploiting Amidated BODIPY-Peptide Conjugates.
Authors: Williams, Tyrslai M and Zhou, Zehua and Singh, Sitanshu S and Sibrian-Vazquez, Martha and Jois, Seetharama D and Henriques Vicente, Maria da Graça
Journal: Photochemistry and photobiology (2020): 581-595

Two π-Electrons Make the Difference: From BODIPY to BODIIM Switchable Fluorescent Dyes.
Authors: Dolati, Hadi and Haufe, Lisa C and Denker, Lars and Lorbach, Andreas and Grotjahn, Robin and Hörner, Gerald and Frank, René
Journal: Chemistry (Weinheim an der Bergstrasse, Germany) (2020): 1422-1428

18F-Labeled BODIPY Dye: A Potential Prosthetic Group for Brain Hybrid PET/Optical Imaging Agents.
Authors: Kim, Hyunjung and Kim, Kyul and Son, Sang-Hyun and Choi, Joon Young and Lee, Kyung-Han and Kim, Byung-Tae and Byun, Youngjoo and Choe, Yearn Seong
Journal: ACS chemical neuroscience (2019): 1445-1451

A Conformationally Restricted Aza-BODIPY Platform for Stimulus-Responsive Probes with Enhanced Photoacoustic Properties.
Authors: Zhou, Effie Y and Knox, Hailey J and Liu, Chang and Zhao, Weili and Chan, Jefferson
Journal: Journal of the American Chemical Society (2019): 17601-17609

A Förster Resonance Energy Transfer Switchable Fluorescent Probe With H2S-Activated Second Near-Infrared Emission for Bioimaging.
Authors: Wang, Rongchen and Gao, Wei and Gao, Jie and Xu, Ge and Zhu, Tianli and Gu, Xianfeng and Zhao, Chunchang
Journal: Frontiers in chemistry (2019): 778

说明书
Bodi Fluor™576/589NHS酯(相当于BODIPY™576/589) CAS 201998-61-0.pdf

ReadiUse™ Preactivated PerCP-Cy7 Maleimide 货号2725-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。

ReadiUse™ Preactivated PerCP-Cy7 Maleimide

ReadiUse™ Preactivated PerCP-Cy7 Maleimide

货号 2725 存储条件 在2-8度冷藏保存, 避免光照
规格 1 mg 价格 4740
Ex (nm) Em (nm)
分子量 N/A 溶剂 Water
产品详细介绍

简要概述

说明书
ReadiUse™ Preactivated PerCP-Cy7 Maleimide.pdf