日度归档：2024年5月27日

Poly(L-lactide) 2.0 dl/g 聚（L-交酯） 2.0 dl/g 品牌：Chemodex CAS No.：26161-42-2

发表于由

品牌：Chemodex
CAS No.：26161-42-2
储存条件：+4°C
纯度：<0.5% (water)
产品编号

(生产商编号)

 等级 规格 运输包装 零售价(RMB) 库存情况 参考值

CDX-P0016-G010

 – 10 g – 1,790.00 – –


* 干冰运输、大包装及大批量的产品需酌情添加运输费用


* 零售价、促销产品折扣、运输费用、库存情况、产品及包装规格可能因各种原因有所变动，恕不另行通知，确切详情请联系上海金畔生物科技有限公司客服。

产品描述相关资料下载相关产品浏览记录 请联系客服

热电/Thermofisher_EL0011_T4 DNA Ligase (5 U/µL)（试剂）_1000 units/瓶 –

发表于由

热电/Thermofisher_EL0011_T4 DNA Ligase (5 U/µL)（试剂）_1000 units/瓶 -

代理商T4 DNA Ligase (5 U/µL)（试剂）_1000 units/瓶

1000 units/瓶

  • 原厂型号

  • 品牌

  • 单件重量

  • 货期

商品信息 商品参数 评价(0)
Thermo Scientific T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5′-phosphate and 3′-hydroxyl termini in duplex DNA or RNA. The enzyme repairs single-strand nicks in duplex DNA, RNA, or DNA/RNA hybrids. It also joins DNA fragments with either cohesive or blunt termini, but has no activity on single-stranded nucleic acids.

T4 DNA Ligase requires ATP as a cofactor.

Highlights

• Active in Themo Scientific restriction enzyme, PCR, and RT buffers (when supplemented with ATP)
• Fast—sticky-end ligation is completed in 10 minutes at room temperature
• Supplied with PEG solution for efficient blunt-end ligation

Applications

• Cloning of restriction enzyme generated DNA fragments
• Cloning of PCR products
• Joining of double-stranded oligonucleotide linkers or adaptors to DNA
• Site-directed mutagenesis
• Amplified fragment length polymorphism (AFLP)
• Ligase-mediated RNA detection (see Reference 3)
• Nick repair in duplex DNA, RNA or DNA/RNA hybrids
• Self-circularization of linear DNA.

Includes

• T4 DNA Ligase
• 10X T4 DNA Ligase Buffer
• 50% PEG Solution

Notes

• Binding of T4 DNA Ligase to DNA may result in a band shift in agarose gels. To avoid this, incubate samples with 6X DNA Loading Dye & SDS Solution at 70°C for 5 min or 65°C for 10 minutes and chill on ice prior to electrophoresis.
• The volume of the ligation reaction mixture should not exceed 10% of the competent cell volume in the transformation process.
• Prior to electro-transformation, remove T4 DNA Ligase from the ligation mixture using spin columns or chloroform extraction. The extracted DNA can be further precipitated with ethanol

无参数