日度归档：2024年10月16日

Thermo Scientific_61002_Dynabeads Oligo(dT)25试剂_2ml 常温 –

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Thermo Scientific_61002_Dynabeads Oligo(dT)25试剂_2ml 常温 -

代理商Dynabeads Oligo(dT)25试剂_2ml 常温

2ml 常温

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描述

Dynabeads® Oligo(dT)25 mRNA isolation beads specifically target and capture mRNA molecules from virtually any crude sample and eliminate the need to purify total RNA when the desired information-bearing nucleic acid is mRNA. Since mRNA comprises only about 1–5% ot total cellular RNA, the isolation of total RNA is not the most efficient way to isolate mRNA. Other technologies designed to purify total RNA yield ~80% ribosomal RNA and force mRNA to compete with ribosomal RNA, transfer RNA, micro RNA, small nucleolar RNA, and small cytoplasmic RNA for membrane binding. Advantages of Dynabeads® Oligo(dT)25 beads:

• Fast and gentle procedure yields pure intact mRNA
• Extremely pure mRNA isolation, best choice upstream of cDNA synthesis
• Exquisitely sensitive mRNA isolation enables cDNA synthesis and cDNA library construction from ultra-small starting samples (enables cDNA library construction from a single cell)

How the beads work
The oligo(dT)25-coated Dynabeads® specifically target and capture the mRNA transcriptome from an extremely wide variety of crude starting samples. Ribosomal RNA, DNA, proteins, and small RNA molecules (such as transfer RNA, micro RNA, and small nucleolar RNA) do not bind to the beads and are discarded. Only polyadenylated RNA species (mRNA) are captured. Isolated mRNA is pure, eliminating the need for ribosomal RNA subtraction or a post-extraction DNase treatment. This column-free system ensures the highest transcriptome recovery:

• Physical mRNA capture on mobile magnetic beads
• Rapid and gentle magnetic handling procedures
• No mRNA lost during high g-force spins
• No mRNA trapped in column membranes during elution

Applications
mRNA is suitable for all downstream molecular applications, including gene cloning, cDNA synthesis, cDNA library construction, RT-PCR, quantitative RT-PCR, RPA (Ribonuclease Protection Assay), subtractive hybridization, primer extension, SAGE, RACE, and others. The Dynabeads® Oligo(dT)25 mRNA isolation beads are the ideal mRNA purification method prior to cDNA library construction. Use of these beads ensures the highest recovery and enrichment of the transcriptome. These beads capture more of the transcriptome than is possible with methods that integrate a total RNA isolation step upstream of mRNA isolation.

Verastile elution options
Elution can be performed in any volume down to 5 µL. mRNA elution is optional because enzymatic reactions in downstream procedures are not inhibited by presence of Dynabeads®. Additionally, one can perform cDNA synthesis directly on the beads to create a reusable solid-phase cDNA library.

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荧光淬灭剂Tide Quencher 6WS炔烃 货号2098-AAT Bioquest荧光染料

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上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品，欢迎访问AAT Bioquest荧光染料官网了解更多信息。

荧光淬灭剂Tide Quencher 6WS炔烃

荧光淬灭剂Tide Quencher 6WS炔烃

荧光淬灭剂Tide Quencher 6WS炔烃 货号2098 货号 2098 存储条件 在零下15度以下保存, 避免光照
规格 1 mg 价格 3924
Ex (nm) Em (nm)
分子量 976.1 溶剂 DMSO
产品详细介绍

简要概述

产品基本信息

货号：2098

产品名称：荧光淬灭剂Tide Quencher 6WS炔烃

规格：1mg

储存条件：-15℃避光防潮

保质期：24个月

 

产品物理化学光谱特性

分子量：963.26

溶剂：DMSO

Abs(nm)：694

 

产品介绍

TQ6WS被设计为优于Cy5.5，TF6和Alexa Fluor 680的淬灭剂。TQ6WS具有（a）吸收力更强； （b）更高的淬火效率； 和（c）通用的反应形式，具有所需的溶解性，可标记寡核苷酸和肽。 该TQ6WS产品对叠氮化物具有反应性，可用于点击化学。金畔生物是AAT Bioquest的中国代理商，为您提供最优质的荧光淬灭剂Tide Quencher 6WS炔烃。 

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参考文献

A mechanistic model to predict effects of cathepsin B and cystatin C on β-amyloid aggregation and degradation
Authors: Perlenfein, Tyler J and Murphy, Regina M
Journal: Journal of Biological Chemistry (2017): jbc–M117

Real-Time Detection of a Self-Replicating RNA Enzyme
Authors: Olea, Charles and Joyce, Gerald F
Journal: Molecules (2016): 1310

Development of Multi-Parametric/Multimodal Spectroscopy Apparatus for Characterization of Functional Interfaces
Authors: Zhou, Lang and Arugula, Mary and Easley, Christopher J and Shannon, Curtis and Simonian, Aleks and r
Journal: ECS Transactions (2015): 9–16

Maternal serum glycosylated fibronectin as a point-of-care biomarker for assessment of preeclampsia
Authors: Rasanen, Juha and Quinn, Matthew J and Laurie, Amber and Bean, Eric and Roberts, Charles T and Nagalla, Srinivasa R and Gravett, Michael G
Journal: American journal of obstetrics and gynecology (2015): 82–e1

Array of biodegradable microrafts for isolation and implantation of living, adherent cells
Authors: Wang, Yuli and Phillips, Colleen N and Herrera, Gabriela S and Sims, Christopher E and Yeh, Jen Jen and Allbritton, Nancy L
Journal: RSC advances (2013): 9264–9272

Development of SNAP-Tag Fluorogenic Probes for Wash-Free Fluorescence Imaging
Authors: Sun, Xiaoli and Zhang, Aihua and Baker, Brenda and Sun, Luo and Howard, Angela and Buswell, John and Maurel, Damien and Masharina, Anastasiya and Johnsson, Kai and Noren, Christopher J and others
Journal: ChemBioChem (2011): 2217–2226

An improved cell-penetrating, caspase-activatable, near-infrared fluorescent peptide for apoptosis imaging
Authors: Maxwell D, Chang Q, Zhang X, Barnett EM, Piwnica-Worms D.
Journal: Bioconjug Chem (2009): 702

Design of FRET-TaqMan probes for multiplex real-time PCR using an internal positive control
Authors: Jothikumar P, Hill V, Narayanan J.
Journal: Biotechniques (2009): 519

Development of a cell-based hepatitis C virus infection fluorescent resonance energy transfer assay for high-throughput antiviral compound screening
Authors: Yu X, Sainz B, Jr., Uprichard SL.
Journal: Antimicrob Agents Chemother (2009): 4311

Evaluation of tetramethylrhodamine and black hole quencher 1 labeled probes and five commercial amplification mixes in TaqMan real-time RT-PCR assays for respiratory pathogens
Authors: Yang GP, Erdman DD, Tondella ML, Fields BS.
Journal: J Virol Methods (2009): 288

说明书
荧光淬灭剂Tide Quencher 6WS炔烃.pdf

Safflower Oil 红花油 品牌：Wako CAS No.：8001-23-8

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品牌：Wako
CAS No.：8001-23-8
储存条件：室温
纯度：–
产品编号

(生产商编号)

 等级 规格 运输包装 零售价(RMB) 库存情况 参考值

199-15375

 Wako 1st Grade 500 ml – 咨询 – –


* 干冰运输、大包装及大批量的产品需酌情添加运输费用


* 零售价、促销产品折扣、运输费用、库存情况、产品及包装规格可能因各种原因有所变动，恕不另行通知，确切详情请联系上海金畔生物科技有限公司客服。

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6-HEX dipivaloate 品牌：Chemodex CAS No.：1166837-63-3

发表于由

品牌：Chemodex
CAS No.：1166837-63-3
储存条件：+4°C
纯度：>97% (HPLC); < 1% Iso 5
产品编号

(生产商编号)

 等级 规格 运输包装 零售价(RMB) 库存情况 参考值

CDX-C0187-M025

 – 25 mg – 1,400.00 – –


* 干冰运输、大包装及大批量的产品需酌情添加运输费用


* 零售价、促销产品折扣、运输费用、库存情况、产品及包装规格可能因各种原因有所变动，恕不另行通知，确切详情请联系上海金畔生物科技有限公司客服。

产品描述相关资料下载相关产品浏览记录 请联系客服