|
品牌：Enzo
CAS No.： 储存条件：-20℃ 纯度：– |
| 产品编号
(生产商编号) |
等级 | 规格 | 运输包装 | 零售价(RMB) | 库存情况 | 参考值 |
|
ENZ-51037-K025 |
– | 1 Kit | – | 3,070.00 | – | – |
* 干冰运输、大包装及大批量的产品需酌情添加运输费用
* 零售价、促销产品折扣、运输费用、库存情况、产品及包装规格可能因各种原因有所变动，恕不另行通知，确切详情请联系上海金畔生物科技有限公司客服。
产品描述相关资料下载相关产品浏览记录
用于更长时间的活细胞荧光标记，没有明显的毒性，可轻易从染料标记性细胞中去除。可用于细胞活性、细胞毒性、细胞迁移、细胞黏附
应用：荧光显微镜 荧光检测 组分: Cyto-ID® 红色示踪染料, 50 μl
4X 标记缓冲液, 12.5 ml
10X HBSS缓冲液, 25 ml
|
品牌：PMRJ
CAS No.： 储存条件：2-10℃ 纯度：– |
| 产品编号
(生产商编号) |
等级 | 规格 | 运输包装 | 零售价(RMB) | 库存情况 | 参考值 |
|
638-01021 |
– | 10000+EU | – | 咨询 | – | – |
* 干冰运输、大包装及大批量的产品需酌情添加运输费用
* 零售价、促销产品折扣、运输费用、库存情况、产品及包装规格可能因各种原因有所变动，恕不另行通知，确切详情请联系上海金畔生物科技有限公司客服。
产品描述相关资料下载相关产品浏览记录 请联系客服
|
品牌：Enzo
CAS No.： 储存条件：-20℃ 纯度：– |
| 产品编号
(生产商编号) |
等级 | 规格 | 运输包装 | 零售价(RMB) | 库存情况 | 参考值 |
|
BML-SE536-0050 |
– | 50 U | – | 3,930.00 | – | – |
* 干冰运输、大包装及大批量的产品需酌情添加运输费用
* 零售价、促销产品折扣、运输费用、库存情况、产品及包装规格可能因各种原因有所变动，恕不另行通知，确切详情请联系上海金畔生物科技有限公司客服。
产品描述相关资料下载相关产品浏览记录 请联系客服
上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品，欢迎访问AAT Bioquest荧光染料官网了解更多信息。
| 货号 | 17292 | 存储条件 | 在零下15度以下保存, 避免光照 |
| 规格 | 1 mL | 价格 | 1164 |
| Ex (nm) | Em (nm) | ||
| 分子量 | 溶剂 | Water | |
| 产品详细介绍 | |||
简要概述
产品基本信息
货号：17292
产品名称：TAQuest FAST qPCR Master Mix 用于TaqMan探针*高ROX*
规格：1ml
储存条件：-15℃避光防潮
保质期：12个月
产品物理化学光谱特性
溶剂：水
产品介绍
用于 TaqMan 探针的 TAQuest FAST qPCR Master Mix 是一种即用型 2X 溶液，针对 qPCR 和两步法 RT-qPCR 进行了优化，非常适合用于 TaqMan 基因表达分析。预混液与 FAST 条件兼容，因此在 20 uL 反应体积中进行 40 个 PCR 循环，可在 50 分钟内提供结果。预混液提供所有基本成分，包括我们专有的 TAQuest FAST 热启动 Taq DNA 聚合酶和优化的 PCR 缓冲液中的 dNTP，但模板、引物和探针除外。用于 TaqMan 探针的 TAQuest FAST qPCR Master Mix 设计用于使用具有卓越性能的内部阳性对照进行双重反应。预混液可确保所有样品类型（如基因组、质粒、病毒和 cDNA 模板）的 PCR 特异性和灵敏度。该预混液含大量 ROX 参考染料。金畔生物是AAT Bioquest的中国代理商，为您提供最优质的TAQuest FAST qPCR Master Mix 用于TaqMan探针*高ROX*。
适用仪器
| qPCR | |
| 仪器规格 | 基于探针的滤波片 |
样品实验方案
注意 在室温下解冻TAQuest FAST qPCR Master Mix 用于TaqMan探针*高ROX*。 使用前彻底涡旋 qPCR Master Mix。
1. 制备表 1 所示的下列反应混合物之一。
2. 轻轻涡旋混合试剂，然后短暂离心。
3. 在 qPCR 仪器中设置板并按表 2 所示操作。
表 1. 各反应每孔试剂组成
| 成分 | 体积 (25 µL/reaction) | 体积 (50 µL/reaction) | 最终浓度 |
| TAQuest FAST qPCR Master Mix 用于TaqMan探针*高ROX* | 12.5 µL | 25 µL | 1X |
| 上游引物，10 µM | 0.25-2.5 µL | 0.5-5.0 µL | 0.1-1.0 µM |
| 下游引物，10 µM | 0.25-2.5 µL | 0.5-5.0 µL | 0.1-1.0 µM |
| DNA模板 | 1-5 µL | 1-5 µL | 优化的浓度 |
| 无核酸酶水 | 25 µL | 50 µL |
表 2. 热循环参数
| 范围 | 聚合酶激活 | PCR (30-40个循环) | ||
| Hold | 变性 | 退火 | 延伸 | |
| 温度 | 95 °C | 95 °C | 55-65 °C | 68-72 °C |
| 时间 (m:ss) | 0:20 | 0:30 | 1:00 | 1:00 |
参考文献
Resilient SARS-CoV-2 diagnostics workflows including viral heat inactivation.
Authors: Lista, Maria Jose and Matos, Pedro M and Maguire, Thomas J A and Poulton, Kate and Ortiz-Zapater, Elena and Page, Robert and Sertkaya, Helin and Ortega-Prieto, Ana M and Scourfield, Edward and O’Byrne, Aoife M and Bouton, Clement and Dickenson, Ruth E and Ficarelli, Mattia and Jimenez-Guardeño, Jose M and Howard, Mark and Betancor, Gilberto and Galao, Rui Pedro and Pickering, Suzanne and Signell, Adrian W and Wilson, Harry and Cliff, Penelope and Kia Ik, Mark Tan and Patel, Amita and MacMahon, Eithne and Cunningham, Emma and Doores, Katie and Agromayor, Monica and Martin-Serrano, Juan and Perucha, Esperanza and Mischo, Hannah E and Shankar-Hari, Manu and Batra, Rahul and Edgeworth, Jonathan and Zuckerman, Mark and Malim, Michael H and Neil, Stuart and Martinez-Nunez, Rocio Teresa
Journal: PloS one (2021): e0256813
Development of a multiplex TaqMan qPCR assay for simultaneous detection and differentiation of four DNA and RNA viruses from clinical samples of sheep and goats.
Authors: Xu, Xingang and Yang, Feng and Zhang, Qi and Xu, Ying and Huang, Jiali and Fu, Mingzhe and Zhang, Weimin
Journal: Journal of virological methods (2019): 58-64
Evaluation and utilization of preassembled frozen commercial fast real-time qPCR master mixes for detection of cytomegalovirus and BK virus.
Authors: Glover, William A and Atienza, Ederlyn E and Nesbitt, Shannon and Kim, Woo J and Castor, Jared and Cook, Linda and Jerome, Keith R
Journal: Journal of medical virology (2016): 115-9
Frequency-encoded laser-induced fluorescence for multiplexed detection in infrared-mediated quantitative PCR.
Authors: Schrell, Adrian M and Roper, Michael G
Journal: The Analyst (2014): 2695-701
Real-time stability testing of air-dried primers and fluorogenic hydrolysis probes stabilized by trehalose and xanthan.
Authors: Rombach, Markus and Kosse, Dominique and Faltin, Bernd and Wadle, Simon and Roth, Günter and Zengerle, Roland and von Stetten, Felix
Journal: BioTechniques (2014): 151-5
Development of a novel internal positive control for Taqman based assays.
Authors: Hartman, Laurie J and Coyne, Susan R and Norwood, David A
Journal: Molecular and cellular probes (2005): 51-9
[Establishment and application of real-time fluorescence polymerase chain reaction based on the TaqMan probes for detection of Yersinia pestis].
Authors: Li, Wei and Hai, Rong and Yu, Dong-zheng and Zhang, Zhi-kai and Cai, Hong
Journal: Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi (2005): 613-6
[Multiplex PCR for detection and quantification of GM potato event EH92-527-1 in food].
Authors: Tyshko, N V and Sadykova, E O and Grouzdev, D S and Sukhacheva, M V
Journal: Voprosy pitaniia: 57-61
[Multiplex polymerase chain reaction for genetically modified potato event AV43-6-G7 quantification. Proof of efficiency].
Authors: Tyshko, N V and Sadykova, E O and Sukhacheva, M V and Grouzdev, D S
Journal: Voprosy pitaniia: 62-70
上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品，欢迎访问AAT Bioquest荧光染料官网了解更多信息。
![二氢荧光素二乙酸酯[荧光素二乙酸酯] CAS 35340-49-9 货号15203](http://www.gewhatman.cn/wp-content/uploads/2022/05/20220509_627930d06bbe4.svg) |
货号 | 15203 | 存储条件 | 在零下15度以下保存, 避免光照 |
| 规格 | 25 mg | 价格 | 1008 | |
| Ex (nm) | 498 | Em (nm) | 517 | |
| 分子量 | 418.4 | 溶剂 | DMSO | |
| 产品详细介绍 | ||||
简要概述
产品基本信息
货号：15203
产品名称：二氢荧光素二乙酸酯[荧光素二乙酸酯]
CAS：35340-49-9
规格：25mg
储存条件：-15℃避光防潮
保质期：12个月
产品物理化学光谱特性
分子量：418.4
溶剂：DMSO
激发波长（nm）：490
发射波长（nm）：514
产品介绍
二氢荧光素二乙酸酯[荧光素二乙酸酯]是美国AAT Bioquest生产的用于检测荧光素的试剂，二氢醋酸荧光素（也称二醋酸酯荧光素）被细胞酯酶水解成二氢荧光素，然后主要通过双氧水氧化产生荧光素。在氧化应激期间二氢醋酸荧光素可能增加大范围的氧化反应。细胞加载研究指出二氢荧光素比其他氧化反应获得更高的胞内浓度（例如2,7-双氯四氟双烷和罗丹明123）。金畔生物是AAT Bioquest的中国代理商，为您提供最优质的二氢荧光素二乙酸酯[荧光素二乙酸酯]。
点击查看光谱
参考文献
Kinetic analysis of fluorescein and dihydrofluorescein effluxes in tumour cells expressing the multidrug resistance protein, MRP1
Authors: Saengkhae C, Loetchutinat C, Garnier-Suillerot A.
Journal: Biochem Pharmacol (2003): 969
Mitochondrial localization of reactive oxygen species by dihydrofluorescein probes
Authors: Diaz G, Liu S, Isola R, Diana A, Falchi AM.
Journal: Histochem Cell Biol (2003): 319
Dihydrofluorescein diacetate is superior for detecting intracellular oxidants: comparison with 2′,7′-dichlorodihydrofluorescein diacetate, 5(and 6)-carboxy-2′,7′-dichlorodihydrofluorescein diacetate, and dihydrorhodamine 123
Authors: Hempel SL, Buettner GR, O’Malley YQ, Wessels DA, Flaherty DM.
Journal: Free Radic Biol Med (1999): 146
A rapid diagnostic test for the viability of early cattle and rabbit embryos using diacetyl-fluorescin
Authors: Schilling E, Dopke HH.
Journal: Naturwissenschaften (1978): 658
Determination of fluorescin sodium in ophthalmic solutions
Authors: Robertson EJ, Patel JA.
Journal: Am J Hosp Pharm (1968): 598
[Experimental research on insulin antibodies. II. Immunochemical characterization of guinea pig anti-ox insulin serum conjugated with fluorescin isothiocyanate.]
Authors: Mancini AM, Zampa GA, Costanzi G.
Journal: Boll Soc Ital Biol Sper (1961): 1230
[Experimental research on insulin antibodies. III. Immunohistology of the endocrine pancreas with guinea pig anti-bovine insulin serum, conjugated with fluorescin isothiocyanate.]
Authors: Costanzi G, Mancini UM, Zampa GA.
Journal: Boll Soc Ital Biol Sper (1961): 1233
Two simple media for the demonstration of pyocyanin and fluorescin
Authors: King EO, Ward MK, Raney DE.
Journal: J Lab Clin Med (1954): 301
Influence of the concentration of iron on the production of fluorescin by Pseudomonas aeruginosa
Authors: Totter JR, Moseley FT.
Journal: J Bacteriol (1953): 45
Transfer of fluorescin from Ps. pyocyanea to colonies of other bacteria
Authors: Hurst L, Lowbury EJ.
Journal: J Clin Pathol (1952): 359