TRITC–葡聚糖偶联物 (average MW = ~70K) 货号21708-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。

TRITC–葡聚糖偶联物 (average MW = ~70K)

TRITC–葡聚糖偶联物 (average MW = ~70K)

TRITC–葡聚糖偶联物 (average MW = ~70K)    货号21708 货号 21708 存储条件 在零下15度以下保存, 避免光照
规格 25 mg 价格 2472
Ex (nm) 544 Em (nm) 570
分子量 ~70,000 溶剂 Water
产品详细介绍

简要概述

产品基本信息

货号:21708

产品名称:TRITC–葡聚糖偶联物

规格:25mg

储存条件:-15℃避光防潮

保质期:24个月

 

产品物理化学光谱特性

分子量:N/A

外观:固体

溶剂:水

激发波长(nm):543

发射波长(nm):569

 

产品介绍

TRITC葡聚糖作为荧光示踪化合物用于心血管、微循环、灌注、细胞单层培养和细胞膜通透性的研究,支持血流、膜损伤、血管引流和肾脏清除等过程的测量。它还可用于利用微荧光法进行微循环和细胞通透性研究,并可用于动物的灌注研究。TRITC葡聚糖也被用来研究植物细胞壁的孔隙率和毛细血管的渗透性。血浆蛋白已被证明不与TRITC葡聚糖结合。小分子的TRITC葡聚糖还常被用来测量细胞内吞和细胞连接通透性等过程。20kDa的TRITC葡聚糖可用于研究细胞过程(如内吞作用和渗透作用)、内皮或上皮单层孔隙率、细胞层内的顶端基底外侧运动、细胞连接通透性和药物从结构(如水凝胶和胶原微棒)中的释放。金畔生物是AAT Bioquest的中国代理商,为您提供最优质的TRITC–葡聚糖偶联物。 

点击查看光谱

 

参考文献

SIRB, sans iron oxide rhodamine B, a novel cross-linked dextran nanoparticle, labels human neuroprogenitor and SH-SY5Y neuroblastoma cells and serves as a USPIO cell labeling control
Authors: Shen, W. B., Vaccaro, D. E., Fishman, P. S., Groman, E. V., Yarowsky, P.
Journal: Contrast Media Mol Imaging (2016): 222-8

In vivo retrograde labeling of neurons in the zebrafish embryo or larva with rhodamine dextran
Authors: Volkmann, K., Koster, R. W.
Journal: CSH Protoc (2007): pdb prot4832

Tracing of axonal connectivities in a combined slice preparation of rat brains–a study by rhodamine-dextran-amine-application in the lateral nucleus of the amygdala
Authors: von Bohlen und Halbach, O., Albrecht, D.
Journal: J Neurosci Methods (1998): 169-75

Multiple anterograde tracing, combining Phaseolus vulgaris leucoagglutinin with rhodamine- and biotin-conjugated dextran amine
Authors: Dolleman-Van der Weel, M. J., Wouterlood, F. G., Witter, M. P.
Journal: J Neurosci Methods (1994): 9-21

Tracing of axonal connections by rhodamine-dextran-amine in the rat hippocampal-entorhinal cortex slice preparation
Authors: Boulton, C. L., von Haebler, D., Heinemann, U.
Journal: Hippocampus (1992): 99-106

Labeling of developing vascular endothelium after injections of rhodamine-dextran into blastomeres of Xenopus laevis
Authors: Rovainen, C. M.
Journal: J Exp Zool (1991): 209-21

In vivo anterograde and retrograde axonal transport of the fluorescent rhodamine-dextran-amine, Fluoro-Ruby, within the CNS
Authors: Schmued, L., Kyriakidis, K., Heimer, L.
Journal: Brain Res (1990): 127-34

说明书
TRITC–葡聚糖偶联物 (average MW = ~70K).pdf

钙离子荧光探针Cal-520-葡聚糖偶联物*MW10000* 货号20601-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。

钙离子荧光探针Cal-520-葡聚糖偶联物*MW10000*

钙离子荧光探针Cal-520-葡聚糖偶联物*MW10000*

钙离子荧光探针Cal-520-葡聚糖偶联物*MW10000*    货号20601 货号 20601 存储条件 在零下15度以下保存, 避免光照
规格 5 mg 价格 3924
Ex (nm) 493 Em (nm) 515
分子量 ~11000 溶剂 Water
产品详细介绍

简要概述

产品基本信息

货号:20601

产品名称:钙离子荧光探针Cal-520-葡聚糖偶联物*MW10000*

规格:5mg

储存条件:-15℃避光防潮

保质期:24个月

 

产品物理化学光谱特性

分子量:~11000

溶剂:水

激发波长(nm):492

发射波长(nm):514

 

产品介绍

钙离子荧光探针Cal-520-葡聚糖偶联物*MW10000*是美国AAT Bioquest生产的钙离子荧光探针,钙的测量对于许多生物学研究至关重要。荧光探针显示结合钙的光谱响应,使研究人员能够通过荧光显微镜,流式细胞仪,荧光光谱和荧光酶标仪研究细胞内游离钙浓度的变化。使用贴片移液管或显微注射,可以使用这些葡聚糖缀合的钙指示剂的细胞不渗透盐形式物理地加载细胞。使用荧光显微镜测量来自这些细胞的荧光信号。与AM酯形式相比,我们的钙指示剂的葡聚糖形式显示出泄漏和区室化的显着减少。在荧光钙指示剂葡聚糖结合物中,Cal-520葡聚糖结合物可能是最佳选择,因为它们具有高荧光量子产率和钙的大荧光增强。与Oregon Green BAPTA-1葡聚糖相比,Cal-520葡聚糖显示出更低的背景和更大的钙诱导的荧光增强。金畔生物是AAT Bioquest的中国代理商,为您提供最优质的钙离子荧光探针Cal-520-葡聚糖偶联物*MW10000*。 

点击查看光谱

钙离子篇:时间轴式讲解应用于钙离子检测的探针

 

参考文献

Phosphatidylinositol 3, 5-Bisphosphate regulates Ca2+ Transport During Yeast Vacuolar Fusion through Activation of the Ca2+ ATPase Pmc1
Authors: Rutilio A Fratti, Gregory E Miner, Katherine D Sullivan, Annie Guo, Matthew L Starr, Ez Ellis, Brandon Jones
Journal: bioRxiv (2019): 533067

A Critical Period for the Rapid Modification of Synaptic Properties at the VPm Relay Synapse
Authors: Libiao Pan, Junhua Yang, Qian Yang, Xiaomeng Wang, Liya Zhu, Yali Liu, Huifang Lou, Chou Xu, Ying Shen, Hao Wang
Journal: Frontiers in molecular neuroscience (2017)

Advances in Two-Photon Scanning and Scanless Microscopy Technologies for Functional Neural Circuit Imaging
Authors: Simon R Schultz, Caroline S Copeland, Amanda J Foust, Peter Quicke, Renaud Schuck
Journal: Proceedings of the IEEE (2017): 139–157

Bidirectional communication between sensory neurons and osteoblasts in an in vitro co-culture system
Authors: Daisuke Kodama, Takao Hirai, Hisataka Kondo, Kazunori Hamamura, Akifumi Togari
Journal: FEBS Letters (2017)

Ca 2+ signals initiate at immobile IP 3 receptors adjacent to ER-plasma membrane junctions
Authors: Nagendra Babu Thillaiappan, Alap P Chavda, Stephen C Tovey, David L Prole, Colin W Taylor
Journal: Nature Communications (2017): 1505

Calcium Imaging Across Large Areas of Intact Vascular Endothelium Reveals Stimulus-Specific Sensory Cells
Authors: Matthew D Lee, Calum Wilson, John G McCarron
Journal: The FASEB Journal (2017): 1005–8

Calcium transient assays for compound screening with human iPSC-derived cardiomyocytes: Evaluating new tools
Authors: Neil J Daily, Radleigh Santos, Joseph Vecchi, Pinar Kemanli, Tetsuro Wakatsuki
Journal: Journal of evolving stem cell research (2017): 1

Characterization of postsynaptic calcium signals in the pyramidal neurons of anterior cingulate cortex
Authors: Xu-Hui Li, Qian Song, Tao Chen, Min Zhuo
Journal: Molecular Pain (2017): 1744806917719847

Direct measurement of TRPV4 and PIEZO1 activity reveals multiple mechanotransduction pathways in chondrocytes
Authors: M Rocio Servin-Vences, Mirko Moroni, Gary R Lewin, Kate Poole
Journal: eLife (2017): e21074

Expression of the GluA2 subunit of glutamate receptors is required for the normal dendritic differentiation of cerebellar Purkinje cells
Authors: Masahiko Tanaka, Tomomi Senda, Naohide Hirashima
Journal: Neuroscience Letters (2017)

 

相关产品

产品名称 货号
钙离子荧光探针Cal-520-葡聚糖偶联物*MW10000* Cat#20601
钙离子荧光探针Cal-520 , AM Cat#21130
钙离子荧光探针Fluo-8, AM Cat#21080

说明书
钙离子荧光探针Cal-520-葡聚糖偶联物*MW10000*.pdf

TRITC–葡聚糖偶联物 (average MW = ~70K) 货号21708-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。

TRITC–葡聚糖偶联物 (average MW = ~70K)

TRITC–葡聚糖偶联物 (average MW = ~70K)

TRITC–葡聚糖偶联物 (average MW = ~70K)    货号21708 货号 21708 存储条件 在零下15度以下保存, 避免光照
规格 25 mg 价格 2472
Ex (nm) 544 Em (nm) 570
分子量 ~70,000 溶剂 Water
产品详细介绍

简要概述

产品基本信息

货号:21708

产品名称:TRITC–葡聚糖偶联物

规格:25mg

储存条件:-15℃避光防潮

保质期:24个月

 

产品物理化学光谱特性

分子量:N/A

外观:固体

溶剂:水

激发波长(nm):543

发射波长(nm):569

 

产品介绍

TRITC葡聚糖作为荧光示踪化合物用于心血管、微循环、灌注、细胞单层培养和细胞膜通透性的研究,支持血流、膜损伤、血管引流和肾脏清除等过程的测量。它还可用于利用微荧光法进行微循环和细胞通透性研究,并可用于动物的灌注研究。TRITC葡聚糖也被用来研究植物细胞壁的孔隙率和毛细血管的渗透性。血浆蛋白已被证明不与TRITC葡聚糖结合。小分子的TRITC葡聚糖还常被用来测量细胞内吞和细胞连接通透性等过程。20kDa的TRITC葡聚糖可用于研究细胞过程(如内吞作用和渗透作用)、内皮或上皮单层孔隙率、细胞层内的顶端基底外侧运动、细胞连接通透性和药物从结构(如水凝胶和胶原微棒)中的释放。金畔生物是AAT Bioquest的中国代理商,为您提供最优质的TRITC–葡聚糖偶联物。 

点击查看光谱

 

参考文献

SIRB, sans iron oxide rhodamine B, a novel cross-linked dextran nanoparticle, labels human neuroprogenitor and SH-SY5Y neuroblastoma cells and serves as a USPIO cell labeling control
Authors: Shen, W. B., Vaccaro, D. E., Fishman, P. S., Groman, E. V., Yarowsky, P.
Journal: Contrast Media Mol Imaging (2016): 222-8

In vivo retrograde labeling of neurons in the zebrafish embryo or larva with rhodamine dextran
Authors: Volkmann, K., Koster, R. W.
Journal: CSH Protoc (2007): pdb prot4832

Tracing of axonal connectivities in a combined slice preparation of rat brains–a study by rhodamine-dextran-amine-application in the lateral nucleus of the amygdala
Authors: von Bohlen und Halbach, O., Albrecht, D.
Journal: J Neurosci Methods (1998): 169-75

Multiple anterograde tracing, combining Phaseolus vulgaris leucoagglutinin with rhodamine- and biotin-conjugated dextran amine
Authors: Dolleman-Van der Weel, M. J., Wouterlood, F. G., Witter, M. P.
Journal: J Neurosci Methods (1994): 9-21

Tracing of axonal connections by rhodamine-dextran-amine in the rat hippocampal-entorhinal cortex slice preparation
Authors: Boulton, C. L., von Haebler, D., Heinemann, U.
Journal: Hippocampus (1992): 99-106

Labeling of developing vascular endothelium after injections of rhodamine-dextran into blastomeres of Xenopus laevis
Authors: Rovainen, C. M.
Journal: J Exp Zool (1991): 209-21

In vivo anterograde and retrograde axonal transport of the fluorescent rhodamine-dextran-amine, Fluoro-Ruby, within the CNS
Authors: Schmued, L., Kyriakidis, K., Heimer, L.
Journal: Brain Res (1990): 127-34

说明书
TRITC–葡聚糖偶联物 (average MW = ~70K).pdf

Cal-590L 葡聚糖偶联物 *MW 10,000* 货号20525-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。

Cal-590L 葡聚糖偶联物 *MW 10,000*

Cal-590L 葡聚糖偶联物 *MW 10,000*

Cal-590L 葡聚糖偶联物 *MW 10,000*    货号20525 货号 20525 存储条件 在零下15度以下保存, 避免光照
规格 1 mg 价格 4740
Ex (nm) 569 Em (nm) 590
分子量 N/A 溶剂 Water
产品详细介绍

简要概述

产品基本信息

货号:20525

产品名称:Cal-590L 葡聚糖偶联物 *MW 10,000*

规格:1mg

储存条件:-15℃避光防潮

保质期:12个月

 

产品物理化学光谱特性

外观:固体

溶剂:水

激发波长(nm):569

发射波长(nm):590

 

产品介绍

钙测量对于许多生物学研究至关重要。在结合钙时显示光谱响应的荧光探针使研究人员能够通过使用荧光显微镜、流式细胞术、荧光光谱和荧光酶标仪来研究细胞内游离钙浓度的变化。可以使用显微注射等方式,用这些葡聚糖缀合的钙指示剂的不透细胞盐形式对细胞进行物理加载。使用荧光显微镜检测来自这些细胞的荧光信号。与AM 酯形式相比,我们的钙指示剂的葡聚糖形式表现出更少的渗漏。在荧光钙指示剂葡聚糖偶联物中,Cal-590L 葡聚糖偶联物可能是比其他红色荧光葡聚糖偶联物更好的选择,因为它具有更高的荧光量子产率和更高的荧光。 Cal-590L 葡聚糖的 Kd ~230 uM。

点击查看光谱

 

参考文献

In vivo nuclear Ca2+-ATPase phosphorylation triggers intermediate size molecular transport to the nucleus.
AuthorsGensburger, Claire and Freyermuth, Solange and Klein, Christian and Malviya, Anant N
JournalBiochemical and biophysical research communications (2003): 1225-8
Real time, confocal imaging of Ca(2+) waves in arterially perfused rat hearts.
AuthorsBaader, Andreas P and Büchler, Lorenz and Bircher-Lehmann, Lilly and Kléber, André G
JournalCardiovascular research (2002): 105-15
Assessment and validation of a microinjection method for kinetic analysis of [Ca2+]i in individual cells undergoing apoptosis.
AuthorsTombal, B and Denmeade, S R and Isaacs, J T
JournalCell calcium (1999): 19-28
Effects of adenosine on Ca2+ entry in the nerve terminal of the frog neuromuscular junction.
AuthorsRobitaille, R and Thomas, S and Charlton, M P
JournalCanadian journal of physiology and pharmacology (1999): 707-14
The nucleus of HeLa cell contains tubular structures for Ca2+ signalling.
AuthorsLui, P P and Kong, S K and Kwok, T T and Lee, C Y
JournalBiochemical and biophysical research communications (1998): 88-93
The rise of nuclear and cytosolic Ca2+ can be uncoupled in HeLa cells.
AuthorsLui, P P and Kong, S K and Fung, K P and Lee, C Y
JournalPflugers Archiv : European journal of physiology (1998): 371-6
Porcine aortic endothelial gap junctions: identification and permeation by caged InsP3.
AuthorsCarter, T D and Chen, X Y and Carlile, G and Kalapothakis, E and Ogden, D and Evans, W H
JournalJournal of cell science (1996): 1765-73
Calcium signals in and around the nucleus in sea urchin eggs.
AuthorsGillot, I and Whitaker, M
JournalCell calcium (1994): 269-78
Spontaneous and propagated calcium release in isolated cardiac myocytes viewed by confocal microscopy.
AuthorsWilliams, D A and Delbridge, L M and Cody, S H and Harris, P J and Morgan, T O
JournalThe American journal of physiology (1992): C731-42

说明书
Cal-590L 葡聚糖偶联物 *MW 10,000*.pdf

新型钙离子荧光探针Calbryte-520XL葡聚糖(停产) 货号20648-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。

新型钙离子荧光探针Calbryte-520XL葡聚糖(停产)

新型钙离子荧光探针Calbryte-520XL葡聚糖(停产)

货号 20648 存储条件 在零下15度以下保存, 避免光照
规格 1 mg 价格 0
Ex (nm) 493 Em (nm) 515
分子量 ~11000 溶剂 Water
产品详细介绍

简要概述

产品基本信息

货号:20648

产品名称:新型钙离子荧光探针Calbryte-520XL葡聚糖

规格:1mg

储存条件:-15℃避光防潮

保质期:24个月

 

产品物理化学光谱特性

分子量:~11000

溶剂:水

激发波长(nm):492

发射波长(nm):514

 

产品介绍

新型钙离子荧光探针Calbryte-520XL葡聚糖是美国AAT Bioquest生产的钙离子荧光探针,Calbryte 520XL右旋糖酐是一种新型荧光且细胞不渗透的钙指示剂,具有极低的亲和力。它的Kd约为500 uM,与著名的Rhod 5N相似,但比Rhod-5N稳定得多。Calbryte 520XL在高浓度钙离子存在下产生明亮的荧光信号。它具有与Fluo-4相同的激发和发射波长,因此相同的Fluo-4测定设置可轻松应用于基于Calbryte 520XL的钙测定。Calbryte 520XL是Rhod-5N的绝佳替代品。我们还提供Calbryte 520XL,AM(#20646),钾盐(#20645)和Calbryte 520XL叠氮化物(#20643),它们可以通过众所周知的点击化学轻松地偶联到载体上。金畔生物是AAT Bioquest的中国代理商,为您提供最优质的新型钙离子荧光探针Calbryte-520XL葡聚糖。 

点击查看光谱

钙离子篇:时间轴式讲解应用于钙离子检测的探针

 

参考文献

Calreticulin regulates TGF-β1-induced epithelial mesenchymal transition through modulating Smad signaling and calcium signaling
Authors: Yanjiao Wu, Xiaoli Xu, Lunkun Ma, Qian Yi, Weichao Sun, Liling Tang
Journal: The International Journal of Biochemistry & Cell Biology (2017)

Dexmedetomidine reduces hypoxia/reoxygenation injury by regulating mitochondrial fission in rat hippocampal neurons
Authors: Jia Liu, Qing Du, He Zhu, Yu Li, Maodong Liu, Shoushui Yu, Shilei Wang
Journal: Int J Clin Exp Med (2017): 6861–6868

Monosialoganglioside 1 may alleviate neurotoxicity induced by propofol combined with remifentanil in neural stem cells
Authors: Jiang Lu, Xue-qin Yao, Xin Luo, Yu Wang, Sookja Kim Chung, He-xin Tang, Chi Wai Cheung, Xian-yu Wang, Chen Meng, Qing Li
Journal: Neural Regeneration Research (2017): 945

Obtaining spontaneously beating cardiomyocyte-like cells from adipose-derived stromal vascular fractions cultured on enzyme-crosslinked gelatin hydrogels
Authors: Gang Yang, Zhenghua Xiao, Xiaomei Ren, Haiyan Long, Kunlong Ma, Hong Qian, Yingqiang Guo
Journal: Scientific Reports (2017): 41781

Di (2-ethylhexyl) phthalate-induced apoptosis in rat INS-1 cells is dependent on activation of endoplasmic reticulum stress and suppression of antioxidant protection
Authors: Xia Sun, Yi Lin, Qiansheng Huang, Junpeng Shi, Ling Qiu, Mei Kang, Yajie Chen, Chao Fang, Ting Ye, Sijun Dong
Journal: Journal of cellular and molecular medicine (2015): 581–594

The effect of mitochondrial calcium uniporter on mitochondrial fission in hippocampus cells ischemia/reperfusion injury
Authors: Lantao Zhao, Shuhong Li, Shilei Wang, Ning Yu, Jia Liu
Journal: Biochemical and biophysical research communications (2015): 537–542

Fungus induces the release of IL-8 in human corneal epithelial cells, via Dectin-1-mediated protein kinase C pathways.
Authors: Xu-Dong Peng, Gui-Qiu Zhao, Jing Lin, Nan Jiang, Qiang Xu, Cheng-Cheng Zhu, Jain-Qiu Qu, Lin Cong, Hui Li
Journal: International journal of ophthalmology (2014): 441–447

Propofol and remifentanil at moderate and high concentrations affect proliferation and differentiation of neural stem/progenitor cells
Authors: Qing Li, Jiang Lu, Xianyu Wang
Journal: Neural regeneration research (2014): 2002

Role of mitochondrial calcium uniporter in regulating mitochondrial fission in the cerebral cortexes of living rats
Authors: Nan Liang, Peng Wang, Shilei Wang, Shuhong Li, Yu Li, Jinying Wang, Min Wang
Journal: Journal of Neural Transmission (2014): 593–600

Increased expression of cell adhesion molecule 1 by mast cells as a cause of enhanced nerve–mast cell interaction in a hapten-induced mouse model of atopic dermatitis
Authors: M Hagiyama, T Inoue, T Furuno, T Iino, S Itami, M Nakanishi, H Asada, Y Hosokawa, A Ito
Journal: British Journal of Dermatology (2013): 771–778

 

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说明书
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