标签归档：deaza

7-Deaza-7-Propargylamino-3′-azidomethyl-dATP 货号17090-AAT Bioquest荧光染料

发表于由

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品，欢迎访问AAT Bioquest荧光染料官网了解更多信息。

7-Deaza-7-Propargylamino-3′-azidomethyl-dATP

7-Deaza-7-Propargylamino-3′-azidomethyl-dATP

7-Deaza-7-Propargylamino-3'-azidomethyl-dATP 货号17090 货号 17090 存储条件 在零下15度以下保存, 避免光照
规格 50 nmoles 价格 4992
Ex (nm) Em (nm)
分子量 598.30 溶剂 Water
产品详细介绍

简要概述

产品基本信息

货号：17090

产品名称：7-Deaza-7-Propargylamino-3′-azidomethyl-dATP

规格：50 nmoles

储存条件：-15℃避光防潮

保质期：24个月

 

产品物理化学光谱特性

分子量：598.30

溶剂：水

 

产品介绍

7-Deaza-7-Propargylamino-3′-azidomethyl-dATP是制备用于下一代测序（NGS）的荧光偶联物的关键组成部分。 NGS使用与早期Sanger测序相似的链终止方法，但NGS是通过荧光标记的核苷酸类似物作为扩增反应的可逆终止剂来进行的。 NGS依赖于可逆的DNA聚合阻断，而Sanger测序则使用ddNTP不可逆转的DNA聚合阻断。 NGS的另一个不同特征是通过桥式PCR进行体外克隆扩增以增加待测序分子的数量。在此实验上，片段与固定在固体表面上的引物连接，进行原位扩增，生成具有相同分子的DNA簇。在每个循环中，同时添加可逆终止的四个核苷酸，并通过它们互补的聚合酶掺入。通过将3′-OH基团替换为3′-o-叠氮基甲基，这些核苷酸被化学封闭，以防止聚合酶在每个循环中掺入一个以上的核苷酸。掺入核苷酸后，在不同通道中针对不同碱基测量荧光信号。关于下一循环，洗涤未掺入的核苷酸，并用TCEP去除3’端的化学封锁。一旦收集到荧光信号，就会开始一个新的循环，重复此动态过程，直到完成每个片段的测序为止。总之，NGS测序反应分三个步骤进行：核苷酸的添加，成像和通过荧光团裂解的3′-OH再生。

 

参考文献

Single-tube, highly parallel mutation enrichment in cancer gene panels by use of temperature-tolerant COLD-PCR.
Authors: Castellanos-Rizaldos, Elena and Richardson, Katherine and Lin, Rui and Wu, Grant and Makrigiorgos, Mike G
Journal: Clinical chemistry (2015): 267-77

Transcriptome analysis of Capsicum annuum varieties Mandarin and Blackcluster: assembly, annotation and molecular marker discovery.
Authors: Ahn, Yul-Kyun and Tripathi, Swati and Kim, Jeong-Ho and Cho, Young-Il and Lee, Hye-Eun and Kim, Do-Sun and Woo, Jong-Gyu and Cho, Myeong-Cheoul
Journal: Gene (2014): 494-9

Mutant firefly luciferases with improved specific activity and dATP discrimination constructed by yeast cell surface engineering.
Authors: Fushimi, Tatsuya and Miura, Natsuko and Shintani, Hideya and Tsunoda, Hiroyuki and Kuroda, Kouichi and Ueda, Mitsuyoshi
Journal: Applied microbiology and biotechnology (2013): 4003-11

Termination of DNA synthesis by N6-alkylated, not 3′-O-alkylated, photocleavable 2′-deoxyadenosine triphosphates.
Authors: Wu, Weidong and Stupi, Brian P and Litosh, Vladislav A and Mansouri, Dena and Farley, Demetra and Morris, Sidney and Metzker, Sherry and Metzker, Michael L
Journal: Nucleic acids research (2007): 6339-49

说明书
7-Deaza-7-Propargylamino-3′-azidomethyl-dATP.pdf

Aminopropargyl ddGTP [7-Deaza-7-Propargylamino-2′,3′-dideoxyguanosine-5′-triphosphate] CAS 114748-61-7 货号17076-AAT Bioquest荧光染料

发表于由

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品，欢迎访问AAT Bioquest荧光染料官网了解更多信息。

Aminopropargyl ddGTP [7-Deaza-7-Propargylamino-2′,3′-dideoxyguanosine-5′-triphosphate] CAS 114748-61-7

Aminopropargyl ddGTP [7-Deaza-7-Propargylamino-2′,3′-dideoxyguanosine-5′-triphosphate] CAS 114748-61-7

Aminopropargyl ddGTP [7-Deaza-7-Propargylamino-2',3'-dideoxyguanosine-5'-triphosphate] CAS 114748-61-7 货号17076 货号 17076 存储条件 在零下15度以下保存, 避免光照
规格 10 umoles 价格 18288
Ex (nm) Em (nm)
分子量 631.19 溶剂 DMF
产品详细介绍

简要概述

产品基本信息

货号：17076

产品名称：Aminopropargyl ddGTP [7-Deaza-7-Propargylamino-2′,3′-dideoxyguanosine-5′-triphosphate]

CAS：114748-61-7

规格：10 umoles

储存条件：-15℃避光防潮

保质期：24个月

 

产品物理化学光谱特性

分子量：631.19

外观：液体

溶剂：DMF

激发波长(nm)：N/A

发射波长(nm)：N/A

 

产品介绍

Sanger法是DNA测序中最早最靠谱的方法之一，DNA由四种脱氧核苷酸三磷酸（dNTP）合成。将每个新核苷酸添加到最后的dNTP的3′-OH基团中。可以将二脱氧胸苷三磷酸（ddTTPs）添加到正在生长的DNA链中，但是当它出现时，链延长会停止，因为下一个要连接的核苷酸没有3′-OH。待测序的DNA被制备成单链。该模板DNA带有大量dATP，dGTP，dCTP和dTTP的混合物。加入四种双脱氧核苷酸（ddATP，ddGTP，ddCTP和ddTTP）的混合物，每种混合物均以限量存在，并分别用发不同颜色的荧光的“标签​​”标记。因为所有四个正常核苷酸都存在，所以链延伸正常进行，直到偶然地DNA聚合酶插入ddNTP（而不是正常dNTP）。如果正常核苷酸与双脱氧形式的比率足够高，则在插入ddNTP之前，某些DNA链将成功添加数百个核苷酸，从而终止该过程。在孵育期结束时，片段的长度从最长到最短分离，一个核苷酸之间的差异足以使该链与下一较短和下一较长链分开。当被激光束照射时，四个DDNTP中的每一个都会发出不同的荧光，可以通过自动扫描仪打印输出序列。这些ddATP，ddGTP，ddCTP和ddTTP胺衍生物是开发Sanger测序试剂的重要组成部分。金畔生物是AAT Bioquest的中国代理商，为您提供最优质的Aminopropargyl ddGTP [7-Deaza-7-Propargylamino-2′,3′-dideoxyguanosine-5′-triphosphate]。 

 

参考文献

Polyadenylated sequencing primers enable complete readability of PCR amplicons analyzed by dideoxynucleotide sequencing
Authors: Beranek, M., Drastikova, M., Petera, J.
Journal: Acta Medica (Hradec Kralove) (2012): 160-4

UV-induced bond modifications in thymine and thymine dideoxynucleotide: structural elucidation of isomers by differential mobility mass spectrometry
Authors: St-Jacques, A., Anichina, J., Schneider, B. B., Covey, T. R., Bohme, D. K.
Journal: Anal Chem (2010): 6163-7

Analysis of processivity of mungbean dideoxynucleotide-sensitive DNA polymerase and detection of the activity and expression of the enzyme in the meristematic and meiotic tissues and following DNA damaging agent
Authors: Roy, S., Choudhury, S. R., Sengupta, D. N.
Journal: Arch Biochem Biophys (2008): 55-65

A dideoxynucleotide-sensitive DNA polymerase activity characterized from endoreduplicating cells of mungbean (Vigna radiata L.) during ontogeny of cotyledons
Authors: Roy, S., Sarkar, S. N., Singh, S. K., Sengupta, D. N.
Journal: FEBS J (2007): 2005-23

Mechanism-based suppression of dideoxynucleotide resistance by K65R human immunodeficiency virus reverse transcriptase using an alpha-boranophosphate nucleoside analogue
Authors: Selmi, B., Boretto, J., Sarfati, S. R., Guerreiro, C., Canard, B.
Journal: J Biol Chem (2001): 48466-72

Synthesis of the first ferrocene-labeled dideoxynucleotide and its use for 3′-redox end-labeling of 5′-modified single-stranded oligonucleotides
Authors: Anne, A., Blanc, B., Moiroux, J.
Journal: Bioconjug Chem (2001): 396-405

Improving dideoxynucleotide-triphosphate utilisation by the hyper-thermophilic DNA polymerase from the archaeon Pyrococcus furiosus
Authors: Evans, S. J., Fogg, M. J., Mamone, A., Davis, M., Pearl, L. H., Connolly, B. A.
Journal: Nucleic Acids Res (2000): 1059-66

Structure-based design of Taq DNA polymerases with improved properties of dideoxynucleotide incorporation
Authors: Li, Y., Mitaxov, V., Waksman, G.
Journal: Proc Natl Acad Sci U S A (1999): 9491-6

Characterization of the native and recombinant catalytic subunit of human DNA polymerase gamma: identification of residues critical for exonuclease activity and dideoxynucleotide sensitivity
Authors: Longley, M. J., Ropp, P. A., Lim, S. E., Copel and W. C.
Journal: Biochemistry (1998): 10529-39

Comparative performance of high-density oligonucleotide sequencing and dideoxynucleotide sequencing of HIV type 1 pol from clinical samples
Authors: Gunthard, H. F., Wong, J. K., Ignacio, C. C., Havlir, D. V., Richman, D. D.
Journal: AIDS Res Hum Retroviruses (1998): 869-76

说明书
Aminopropargyl ddGTP [7-Deaza-7-Propargylamino-2′,3′-dideoxyguanosine-5′-triphosphate] CAS 114748-61-7.pdf

Aminopropargyl ddATP [7-Deaza-7-Propargylamino-2′,3′-dideoxyadenosine-5′-triphosphate] CAS 114748-69-5 货号17074-AAT Bioquest荧光染料

发表于由

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品，欢迎访问AAT Bioquest荧光染料官网了解更多信息。

Aminopropargyl ddATP [7-Deaza-7-Propargylamino-2′,3′-dideoxyadenosine-5′-triphosphate] CAS 114748-69-5

Aminopropargyl ddATP [7-Deaza-7-Propargylamino-2′,3′-dideoxyadenosine-5′-triphosphate] CAS 114748-69-5

Aminopropargyl ddATP [7-Deaza-7-Propargylamino-2',3'-dideoxyadenosine-5'-triphosphate] CAS 114748-69-5 货号17074 货号 17074 存储条件 在零下15度以下保存, 避免光照
规格 10 umoles 价格 11988
Ex (nm) Em (nm)
分子量 615.19 溶剂 Water
产品详细介绍

简要概述

产品基本信息

货号：17074

产品名称：Aminopropargyl ddATP [7-Deaza-7-Propargylamino-2′,3′-dideoxyadenosine-5′-triphosphate]

CAS：114748-69-5

规格：10 umoles

储存条件：-15℃避光防潮

保质期：24个月

 

产品物理化学光谱特性

分子量：615.19

外观：液体

溶剂：DMF

激发波长(nm)：N/A

发射波长(nm)：N/A

 

产品介绍

Sanger法是DNA测序中最早最靠谱的方法之一，DNA由四种脱氧核苷酸三磷酸（dNTP）合成。将每个新核苷酸添加到最后的dNTP的3′-OH基团中。可以将二脱氧胸苷三磷酸（ddTTPs）添加到正在生长的DNA链中，但是当它出现时，链延长会停止，因为下一个要连接的核苷酸没有3′-OH。待测序的DNA被制备成单链。该模板DNA带有大量dATP，dGTP，dCTP和dTTP的混合物。加入四种双脱氧核苷酸（ddATP，ddGTP，ddCTP和ddTTP）的混合物，每种混合物均以限量存在，并分别用发不同颜色的荧光的“标签​​”标记。因为所有四个正常核苷酸都存在，所以链延伸正常进行，直到偶然地DNA聚合酶插入ddNTP（而不是正常dNTP）。如果正常核苷酸与双脱氧形式的比率足够高，则在插入ddNTP之前，某些DNA链将成功添加数百个核苷酸，从而终止该过程。在孵育期结束时，片段的长度从最长到最短分离，一个核苷酸之间的差异足以使该链与下一较短和下一较长链分开。当被激光束照射时，四个DDNTP中的每一个都会发出不同的荧光，可以通过自动扫描仪打印输出序列。这些ddATP，ddGTP，ddCTP和ddTTP胺衍生物是开发Sanger测序试剂的重要组成部分。金畔生物是AAT Bioquest的中国代理商，为您提供最优质的Aminopropargyl ddATP [7-Deaza-7-Propargylamino-2′,3′-dideoxyadenosine-5′-triphosphate]。 

 

参考文献

Polyadenylated sequencing primers enable complete readability of PCR amplicons analyzed by dideoxynucleotide sequencing
Authors: Beranek, M., Drastikova, M., Petera, J.
Journal: Acta Medica (Hradec Kralove) (2012): 160-4

UV-induced bond modifications in thymine and thymine dideoxynucleotide: structural elucidation of isomers by differential mobility mass spectrometry
Authors: St-Jacques, A., Anichina, J., Schneider, B. B., Covey, T. R., Bohme, D. K.
Journal: Anal Chem (2010): 6163-7

Analysis of processivity of mungbean dideoxynucleotide-sensitive DNA polymerase and detection of the activity and expression of the enzyme in the meristematic and meiotic tissues and following DNA damaging agent
Authors: Roy, S., Choudhury, S. R., Sengupta, D. N.
Journal: Arch Biochem Biophys (2008): 55-65

A dideoxynucleotide-sensitive DNA polymerase activity characterized from endoreduplicating cells of mungbean (Vigna radiata L.) during ontogeny of cotyledons
Authors: Roy, S., Sarkar, S. N., Singh, S. K., Sengupta, D. N.
Journal: FEBS J (2007): 2005-23

Mechanism-based suppression of dideoxynucleotide resistance by K65R human immunodeficiency virus reverse transcriptase using an alpha-boranophosphate nucleoside analogue
Authors: Selmi, B., Boretto, J., Sarfati, S. R., Guerreiro, C., Canard, B.
Journal: J Biol Chem (2001): 48466-72

Synthesis of the first ferrocene-labeled dideoxynucleotide and its use for 3′-redox end-labeling of 5′-modified single-stranded oligonucleotides
Authors: Anne, A., Blanc, B., Moiroux, J.
Journal: Bioconjug Chem (2001): 396-405

Improving dideoxynucleotide-triphosphate utilisation by the hyper-thermophilic DNA polymerase from the archaeon Pyrococcus furiosus
Authors: Evans, S. J., Fogg, M. J., Mamone, A., Davis, M., Pearl, L. H., Connolly, B. A.
Journal: Nucleic Acids Res (2000): 1059-66

Structure-based design of Taq DNA polymerases with improved properties of dideoxynucleotide incorporation
Authors: Li, Y., Mitaxov, V., Waksman, G.
Journal: Proc Natl Acad Sci U S A (1999): 9491-6

Characterization of the native and recombinant catalytic subunit of human DNA polymerase gamma: identification of residues critical for exonuclease activity and dideoxynucleotide sensitivity
Authors: Longley, M. J., Ropp, P. A., Lim, S. E., Copel and W. C.
Journal: Biochemistry (1998): 10529-39

Comparative performance of high-density oligonucleotide sequencing and dideoxynucleotide sequencing of HIV type 1 pol from clinical samples
Authors: Gunthard, H. F., Wong, J. K., Ignacio, C. C., Havlir, D. V., Richman, D. D.
Journal: AIDS Res Hum Retroviruses (1998): 869-76

说明书
Aminopropargyl ddATP [7-Deaza-7-Propargylamino-2′,3′-dideoxyadenosine-5′-triphosphate] CAS 114748-69-5.pdf

7-Deaza-7-Propargylamino-3′-azidomethyl-dGTP 货号17092-AAT Bioquest荧光染料

发表于由

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品，欢迎访问AAT Bioquest荧光染料官网了解更多信息。

7-Deaza-7-Propargylamino-3′-azidomethyl-dGTP

7-Deaza-7-Propargylamino-3′-azidomethyl-dGTP

7-Deaza-7-Propargylamino-3'-azidomethyl-dGTP 货号17092 货号 17092 存储条件 在零下15度以下保存, 避免光照
规格 50 nmoles 价格 4368
Ex (nm) Em (nm)
分子量 614.30 溶剂 Water
产品详细介绍

简要概述

产品基本信息

货号：17092

产品名称：7-Deaza-7-Propargylamino-3′-azidomethyl-dGTP

规格：50 nmoles

储存条件：-15℃避光防潮

保质期：24个月

 

产品物理化学光谱特性

分子量：614.30

溶剂：水

 

产品介绍

7-Deaza-7-Propargylamino-3′-azidomethylmethyl-dGTP是制备用于下一代测序（NGS）的荧光缀合物的关键组成部分。 NGS使用与早期Sanger测序相似的链终止方法，但NGS是通过荧光标记的核苷酸类似物作为扩增反应的可逆终止剂来进行的。 NGS依赖于可逆的DNA聚合阻断，而Sanger测序则使用ddNTP不可逆转的DNA聚合阻断。 NGS的另一个不同特征是通过桥式PCR进行体外克隆扩增以增加待测序分子的数量。在此平台上，片段与固定在固体表面上的引物连接，进行原位扩增，生成具有相同分子的DNA簇。在每个循环中，同时添加可逆终止的四个核苷酸，并通过它们互补的聚合酶掺入。通过将3′-OH基团替换为3′-o-叠氮基甲基，这些核苷酸被化学封闭，以防止聚合酶在每个循环中掺入一个以上的核苷酸。掺入核苷酸后，在不同通道中针对不同碱基测量荧光信号。关于下一循环，洗涤未掺入的核苷酸，并用TCEP去除3’端的化学封锁。一旦收集到荧光信号，就会开始一个新的循环，重复此动态过程，直到完成每个片段的测序为止。总之，NGS测序反应分三个步骤进行：核苷酸的添加，成像和通过荧光团裂解的3′-OH再生。

 

参考文献

A novel target enrichment strategy in next-generation sequencing through 7-deaza-dGTP-resistant enzymatic digestion.
Authors: Peng, Peng and Xu, Yanjuan and Di Bisceglie, Adrian M and Fan, Xiaofeng
Journal: BMC research notes (2020): 445

SAMHD1-deficient fibroblasts from Aicardi-Goutières Syndrome patients can escape senescence and accumulate mutations.
Authors: Franzolin, Elisa and Coletta, Sara and Ferraro, Paola and Pontarin, Giovanna and D’Aronco, Giulia and Stevanoni, Martina and Palumbo, Elisa and Cagnin, Stefano and Bertoldi, Loris and Feltrin, Erika and Valle, Giorgio and Russo, Antonella and Bianchi, Vera and Rampazzo, Chiara
Journal: FASEB journal : official publication of the Federation of American Societies for Experimental Biology (2020): 631-647

Suppressors of dGTP Starvation in Escherichia coli.
Authors: Itsko, Mark and Schaaper, Roel M
Journal: Journal of bacteriology (2017)

Single-tube, highly parallel mutation enrichment in cancer gene panels by use of temperature-tolerant COLD-PCR.
Authors: Castellanos-Rizaldos, Elena and Richardson, Katherine and Lin, Rui and Wu, Grant and Makrigiorgos, Mike G
Journal: Clinical chemistry (2015): 267-77

Transcriptome analysis of Capsicum annuum varieties Mandarin and Blackcluster: assembly, annotation and molecular marker discovery.
Authors: Ahn, Yul-Kyun and Tripathi, Swati and Kim, Jeong-Ho and Cho, Young-Il and Lee, Hye-Eun and Kim, Do-Sun and Woo, Jong-Gyu and Cho, Myeong-Cheoul
Journal: Gene (2014): 494-9

Deep sequencing analysis of mutations resulting from the incorporation of dNTP analogs.
Authors: Petrie, Katherine L and Joyce, Gerald F
Journal: Nucleic acids research (2010): 8095-104

Design and synthesis of a photocleavable fluorescent nucleotide 3′-O-allyl-dGTP-PC-Bodipy-FL-510 as a reversible terminator for DNA sequencing by synthesis.
Authors: Meng, Qinglin and Kim, Dae Hyun and Bai, Xiaopeng and Bi, Lanrong and Turro, Nicholas J and Ju, Jingyue
Journal: The Journal of organic chemistry (2006): 3248-52

说明书
7-Deaza-7-Propargylamino-3′-azidomethyl-dGTP.pdf