标签归档：dutp

AA-dUTP [氨基烯丙基 dUTP 钠盐] *4 mM Tris 缓冲液 (pH 7.5)* *CAS 936327-10-5* 货号17004-AAT Bioquest荧光染料

发表于由

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品，欢迎访问AAT Bioquest荧光染料官网了解更多信息。

AA-dUTP [氨基烯丙基 dUTP 钠盐] *4 mM Tris 缓冲液 (pH 7.5)* *CAS 936327-10-5*

AA-dUTP [氨基烯丙基 dUTP 钠盐] *4 mM Tris 缓冲液 (pH 7.5)* *CAS 936327-10-5*

AA-dUTP [氨基烯丙基 dUTP 钠盐] *4 mM Tris 缓冲液 (pH 7.5)* *CAS 936327-10-5* 货号17004 货号 17004 存储条件 在零下15度以下保存, 避免光照
规格 1 umole 价格 1272
Ex (nm) Em (nm)
分子量 589.17 溶剂 Water
产品详细介绍

简要概述

产品基本信息

货号：17004

产品名称：AA-dUTP [氨基烯丙基 dUTP 钠盐] *4 mM Tris 缓冲液 (pH 7.5)* *CAS 936327-10-5*

CAS：936327-10-5

规格：1 umole

储存条件：-15℃避光防潮

保质期：12个月

 

产品物理化学光谱特性

分子量：589.17

溶剂：水

激发波长(nm)：N/A

发射波长(nm)：N/A

 

产品介绍

氨基烯丙基-DUTP钠盐 4mM AA-dUTP是美国AAT Bioquest生产的荧光染料，胺修饰的脱氧尿苷5′-三磷酸酯（如氨基烯丙基-dUTP）可用于通过常规酶掺入方法（如反转录，缺口翻译，随机引物标记或PCR）生产含胺的DNA。氨基烯丙基dUTP可以通过常规酶结合技术轻松地掺入DNA中。然后可以使用任何胺反应性荧光染料，生物素和其他胺反应性试剂对所得的胺修饰的核酸进行标记。与通常具有较高立体抑制能力的标签标记的尿苷三磷酸酯相比，氨基烯丙基修饰的核苷酸可以掺入到极高且一致的水平。胺修饰的核酸与过量的胺反应性试剂的随后反应，无论选择何种标记试剂，均能实现相应较高的一致标记效率。这种两步标记方法还消除了优化酶促反应以适应不同染料修饰核苷酸的需要，该修饰可能以非常不同的速率掺入。这种标记方法广泛用于FISH探针和基于微阵列的实验。金畔生物是AAT Bioquest的中国代理商，为您提供最优质的氨基烯丙基-DUTP钠盐 4mM AA-dUTP 。

 

参考文献

A visual DNA chip for simultaneous detection of hepatitis B virus, hepatitis C virus and human immunodeficiency virus type-1
Authors: Wen JK, Zhang XE, Cheng Z, Liu H, Zhou YF, Zhang ZP, Yang JH, Deng JY.
Journal: Biosens Bioelectron (2004): 685

Fluorescent DNA hybridization probe preparation using amine modification and reactive dye coupling
Authors: Cox WG, Singer VL.
Journal: Biotechniques (2004): 114

Aminomodified nucleobases: functionalized nucleoside triphosphates applicable for SELEX
Authors: Schoetzau T, Langner J, Moyroud E, Roehl I, Vonhoff S, Klussmann S.
Journal: Bioconjug Chem (2003): 919

Simple method for preparation of fluor/hapten-labeled dUTP
Authors: Nimmakayalu M, Henegariu O, Ward DC, Bray-Ward P.
Journal: Biotechniques (2000): 518

Topology of yeast RNA polymerase II subunits in transcription elongation complexes studied by photoaffinity cross-linking
Authors: Wooddell CI, Burgess RR.
Journal: Biochemistry (2000): 13405

Quantitative analysis of polymerase chain reaction products using biotinylated dUTP incorporation
Authors: Duplaa C, Couffinhal T, Labat L, Moreau C, Lamaziere JM, Bonnet J.
Journal: Anal Biochem (1993): 229

A non-radioisotopic reverse transcriptase assay using biotin-11-deoxyuridinetriphosphate on primer-immobilized microtiter plates
Authors: Urabe T, Sano K, Tanno M, Mizoguchi J, Otani M, Lee MH, Takasaki T, Kusakabe H, Imagawa DT, Nakai M.
Journal: J Virol Methods (1992): 145

Affinity isolation of active murine erythroleukemia cell chromatin: uniform distribution of ubiquitinated histone H2A between active and inactive fractions
Authors: Dawson BA, Herman T, Haas AL, Lough J.
Journal: J Cell Biochem (1991): 166

Non-radioactive labeling and detection of nucleic acids. IV. Synthesis and properties of digoxigenin-modified 2′-deoxyuridine-5′-triphosphates and a photoactivatable analog of digoxigenin (photodigoxigenin)
Authors: Muhlegger K, Huber E, von der Eltz H, Ruger R, Kessler C.
Journal: Biol Chem Hoppe Seyler (1990): 953

说明书
AA-dUTP [氨基烯丙基 dUTP 钠盐] *4 mM Tris 缓冲液 (pH 7.5)* *CAS 936327-10-5*.pdf

5-Propargylamino-3′-azidomethyl-dUTP 货号17093-AAT Bioquest荧光染料

发表于由

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品，欢迎访问AAT Bioquest荧光染料官网了解更多信息。

5-Propargylamino-3′-azidomethyl-dUTP

5-Propargylamino-3′-azidomethyl-dUTP

5-Propargylamino-3'-azidomethyl-dUTP 货号17093 货号 17093 存储条件 在零下15度以下保存, 避免光照
规格 50 nmoles 价格 3108
Ex (nm) Em (nm)
分子量 576.24 溶剂 Water
产品详细介绍

简要概述

产品基本信息

货号：17093

产品名称：5-Propargylamino-3′-azidomethyl-dUTP

规格：50 nmoles

储存条件：-15℃避光防潮

保质期：24个月

 

产品物理化学光谱特性

分子量：576.24

溶剂：水

 

产品介绍

5-Propargylamino-3′-azidomethyl-dUTP是制备用于下一代测序（NGS）的荧光缀合物的关键组成部分。 NGS使用与早期Sanger测序相似的链终止方法，但NGS是通过荧光标记的核苷酸类似物作为扩增反应的可逆终止剂来进行的。 NGS依赖于可逆的DNA聚合阻断，而Sanger测序则使用ddNTP不可逆转的DNA聚合阻断。 NGS的另一个不同特征是通过桥式PCR进行体外克隆扩增以增加待测序分子的数量。在此平台上，片段与固定在固体表面上的引物连接，进行原位扩增，生成具有相同分子的DNA簇。在每个循环中，同时添加可逆终止的四个核苷酸，并通过它们互补的聚合酶掺入。通过将3′-OH基团替换为3′-o-叠氮基甲基，这些核苷酸被化学封闭，以防止聚合酶在每个循环中掺入一个以上的核苷酸。掺入核苷酸后，在不同通道中针对不同碱基测量荧光信号。关于下一循环，洗涤未掺入的核苷酸，并用TCEP去除3’端的化学封锁。一旦收集到荧光信号，就会开始一个新的循环，重复此动态过程，直到完成每个片段的测序为止。总之，NGS测序反应分三个步骤进行：核苷酸的添加，成像和通过荧光团裂解的3′-OH再生。

 

参考文献

Application of Next Generation Sequencing in Laboratory Medicine.
Authors: Zhong, Yiming and Xu, Feng and Wu, Jinhua and Schubert, Jeffrey and Li, Marilyn M
Journal: Annals of laboratory medicine (2021): 25-43

Current scenario of the genetic testing for rare neurological disorders exploiting next generation sequencing.
Authors: Di Resta, Chiara and Pipitone, Giovanni Battista and Carrera, Paola and Ferrari, Maurizio
Journal: Neural regeneration research (2021): 475-481

A New Type of Chronic Wound Infection after Wisdom Tooth Extraction: A Diagnostic Approach with 16S-rRNA Gene Analysis, Next-Generation Sequencing, and Bioinformatics.
Authors: Böttger, Sebastian and Zechel-Gran, Silke and Streckbein, Philipp and Knitschke, Michael and Hain, Torsten and Weigel, Markus and Wilbrand, Jan-Falco and Domann, Eugen and Howaldt, Hans-Peter and Attia, Sameh
Journal: Pathogens (Basel, Switzerland) (2020)

A novel Next-Generation Sequencing-based approach for concurrent detection of mitochondrial DNA copy number and mutation.
Authors: Zhou, Kaixiang and Mo, Qinqin and Guo, Shanshan and Liu, Yang and Yin, Chun and Ji, Xiaoying and Guo, Xu and Xing, Jinliang
Journal: The Journal of molecular diagnostics : JMD (2020)

Amplicon-Based Next-Generation Sequencing for Detection of Fungi in Formalin-Fixed, Paraffin-Embedded Tissues: Correlation with Histopathology and Clinical Applications.
Authors: Larkin, Paige M K and Lawson, Katy L and Contreras, Deisy A and Le, Catherine Q and Trejo, Marisol and Realegeno, Susan and Hilt, Evann E and Chandrasekaran, Sukantha and Garner, Omai B and Fishbein, Gregory A and Yang, Shangxin
Journal: The Journal of molecular diagnostics : JMD (2020): 1287-1293

Analytical performance evaluation of a commercial next generation sequencing liquid biopsy platform using plasma ctDNA, reference standards, and synthetic serial dilution samples derived from normal plasma.
Authors: Verma, Suman and Moore, Mathew W and Ringler, Rebecca and Ghosal, Abhisek and Horvath, Kyle and Naef, Theodore and Anvari, Sheri and Cotter, Philip D and Gunn, Shelly
Journal: BMC cancer (2020): 945

Author Correction: Ultrasensitive amplicon barcoding for next-generation sequencing facilitating sequence error and amplification-bias correction.
Authors: Ahmed, Ibrahim and Tucci, Felicia A and Aflalo, Aure and Smith, Kenneth G C and Bashford-Rogers, Rachael J M
Journal: Scientific reports (2020): 17010

Automation of Amplicon-Based Library Preparation for Next-Generation Sequencing by Centrifugal Microfluidics.
Authors: Hess, Jacob Friedrich and Kotrová, Michaela and Calabrese, Silvia and Darzentas, Nikos and Hutzenlaub, Tobias and Zengerle, Roland and Brüggemann, Monika and Paust, Nils
Journal: Analytical chemistry (2020): 12833-12841

Characterization of the novel HLA-B*15:474 allele by next-generation sequencing.
Authors: Genebrier, Steve and Elsermans, Vincent and Texeraud, Emeric and Bertrand, Gerald and Renac, Virginie
Journal: HLA (2020)

Correction to: Malignant struma ovarii: next-generation sequencing of six cases revealed Nras, Braf, and Jak3 mutations.
Authors: Poli, Roberta and Scatolini, Maria and Grosso, Enrico and Maletta, Francesca and Gallo, Marco and Liscia, Daniele and Nelva, Anna and Cesario, Flora and Forte, Giuseppe and Metovic, Jasna and Volante, Marco and Arvat, Emanuela and Papotti, Mauro
Journal: Endocrine (2020)

说明书
5-Propargylamino-3′-azidomethyl-dUTP.pdf

iFluor 488-dUTP * 1 mM的Tris缓冲液（pH 7.5）* 货号17039-AAT Bioquest荧光染料

发表于由

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品，欢迎访问AAT Bioquest荧光染料官网了解更多信息。

iFluor 488-dUTP * 1 mM的Tris缓冲液（pH 7.5）*

iFluor 488-dUTP * 1 mM的Tris缓冲液（pH 7.5）*

iFluor 488-dUTP * 1 mM的Tris缓冲液（pH 7.5）* 货号17039 货号 17039 存储条件 在零下15度以下保存, 避免光照
规格 25 nmoles 价格 1164
Ex (nm) 491 Em (nm) 516
分子量 1152.83 溶剂 Water
产品详细介绍

简要概述

产品基本信息

货号：17039

产品名称：iFluor 488-dUTP

规格：25 nmoles

储存条件：-15℃避光防潮

保质期：24个月

 

产品物理化学光谱特性

分子量：1152.83

溶剂：水

激发波长(nm)：491

发射波长(nm)：516

 

产品介绍

染料修饰的脱氧尿苷5′-三磷酸酯是通过常规酶掺入方法（例如反转录，缺口翻译，随机引物标记或PCR）生产染料标记DNA的最常用方法之一。 这种酶促荧光标记方法广泛用于FISH探针和基于微阵列的实验。 该iFluor 488-dUTP缀合物与SpectrumGreen 滤光片组合一起用作绿色荧光（SpectrumGreen 是Vysis的商标）。 与常用的绿色探针（例如Fluorescein-12-dUTP）相比，iFluor 488提供了更明亮的信号，具有很高的光稳定性，并且不受pH值的影响。

点击查看光谱

 

参考文献

Anti-proliferative effect of Fe(III) complexed with 1-(2-hydroxy-3-methoxybenzaldehyde)-4-aminosalicylhydrazone in HepG2 cells.
Authors: Fukushima, Takeshi and Taniguchi, Erina and Yamada, Hiroshi and Kato, Kiyomasa and Shimizu, Ayako and Nishiguchi, Yoshikazu and Onozato, Mayu and Ichiba, Hideaki and Azuma, Yutaro
Journal: Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine (2015): 669-77

Localisation of abundant and organ-specific genes expressed in Rosa hybrida leaves and flower buds by direct in situ RT-PCR.
Authors: Jedrzejuk, Agata and Mibus, Heiko and Serek, Margrethe
Journal: TheScientificWorldJournal (2012): 609597

SNP genotyping using microsphere-linked PNA and flow cytometric detection.
Authors: Rockenbauer, Eszter and Petersen, Kenneth and Vogel, Ulla and Bolund, Lars and Kølvraa, Steen and Nielsen, Kirsten Vang and Nexø, Bjørn A
Journal: Cytometry. Part A : the journal of the International Society for Analytical Cytology (2005): 80-6

[Comparative genomic hybridization analysis of nasopharyngeal carcinoma drug-resistant cell CNE2/DDP and its parent cell CNE2].
Authors: Jiang, Run-De and Hu, Liang and Guan, Xin-Yuan and Zhang, Li-Xin and Yue, Wen and Cen, Xin-Tang and Li, Chun-Hai
Journal: Ai zheng = Aizheng = Chinese journal of cancer (2004): 386-90

Flow cytometric detection of beta-D-glucuronidase gene in wild-type bacterial cells using in-situ PCR.
Authors: Sachidanandham, Ramaiah and Gin, Karina Yew-Hoong
Journal: Biotechnology and bioengineering (2003): 127-33

Lack of nuclear apoptosis in cardiomyocytes and increased endothelin-1 levels in a rat heart model of myocardial stunning.
Authors: Klainguti, M and Aigner, S and Kilo, J and Eppenberger, H M and Mandinova, A and Aebi, U and Schaub, M C and Shaw, S G and Lüscher, T F and Atar, D
Journal: Basic research in cardiology (2000): 308-15

A method to monitor DNA transfer during transfection.
Authors: Johnson, A L and Jurcisek, J A and Trask, O J and Au, J L
Journal: AAPS pharmSci (1999): E6

Pulsatile shear stress leads to DNA fragmentation in human SH-SY5Y neuroblastoma cell line.
Authors: Triyoso, D H and Good, T A
Journal: The Journal of physiology (1999): 355-65

Detection of TNF alpha and Fas ligand mRNA within synovial mononuclear cells by fluorescence in-cell labeling PCR (FICL-PCR).
Authors: Okubo, M and Brown, M P and Chiba, K and Kasukawa, R and Nishimaki, T
Journal: Molecular biology reports (1998): 217-24

Targeted nucleotide exchange in the alkaline phosphatase gene of HuH-7 cells mediated by a chimeric RNA/DNA oligonucleotide.
Authors: Kren, B T and Cole-Strauss, A and Kmiec, E B and Steer, C J
Journal: Hepatology (Baltimore, Md.) (1997): 1462-8

说明书
iFluor 488-dUTP * 1 mM的Tris缓冲液（pH 7.5）*.pdf

1mM mFluor dUTP溶液 货号17011-AAT Bioquest荧光染料

发表于由

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品，欢迎访问AAT Bioquest荧光染料官网了解更多信息。

1mM mFluor dUTP溶液

1mM mFluor dUTP溶液

1mM mFluor dUTP溶液 货号17011 货号 17011 存储条件 在零下15度以下保存, 避免光照
规格 25 nmoles 价格 3924
Ex (nm) 406 Em (nm) 445
分子量 ~1000 溶剂 Water
产品详细介绍

简要概述

产品基本信息

货号：17011

产品名称：1mM mFluor dUTP溶液

规格：25 nmoles

储存条件：-15℃避光防潮

保质期：12个月

 

产品物理化学光谱特性

分子量：~1000

溶剂：水

激发波长(nm)：405

发射波长(nm)：444

 

产品介绍

1mM mFluor dUTP溶液是美国AAT Bioquest生产的荧光染料，染料修饰的脱氧尿苷5′-三磷酸酯（例如氨基烯丙基-dUTP）可用于通过常规酶促掺入方法（例如逆转录，缺口翻译，随机引物标记或PCR）生产含染料的DNA。这种酶促荧光标记方法广泛用于FISH探针和基于微阵列的实验。该mFluor 450-dUTP偶联物可与PacificBlue®滤光片组一起用作蓝色荧光色。（PacificBlue®是Invitrogen的商标）。金畔生物是AAT Bioquest的中国代理商，为您提供最优质的1mM mFluor dUTP溶液。

点击查看光谱

 

参考文献

A visual DNA chip for simultaneous detection of hepatitis B virus, hepatitis C virus and human immunodeficiency virus type-1
Authors: Wen JK, Zhang XE, Cheng Z, Liu H, Zhou YF, Zhang ZP, Yang JH, Deng JY.
Journal: Biosens Bioelectron (2004): 685

Fluorescent DNA hybridization probe preparation using amine modification and reactive dye coupling
Authors: Cox WG, Singer VL.
Journal: Biotechniques (2004): 114

Aminomodified nucleobases: functionalized nucleoside triphosphates applicable for SELEX
Authors: Schoetzau T, Langner J, Moyroud E, Roehl I, Vonhoff S, Klussmann S.
Journal: Bioconjug Chem (2003): 919

Simple method for preparation of fluor/hapten-labeled dUTP
Authors: Nimmakayalu M, Henegariu O, Ward DC, Bray-Ward P.
Journal: Biotechniques (2000): 518

Topology of yeast RNA polymerase II subunits in transcription elongation complexes studied by photoaffinity cross-linking
Authors: Wooddell CI, Burgess RR.
Journal: Biochemistry (2000): 13405

Quantitative analysis of polymerase chain reaction products using biotinylated dUTP incorporation
Authors: Duplaa C, Couffinhal T, Labat L, Moreau C, Lamaziere JM, Bonnet J.
Journal: Anal Biochem (1993): 229

A non-radioisotopic reverse transcriptase assay using biotin-11-deoxyuridinetriphosphate on primer-immobilized microtiter plates
Authors: Urabe T, Sano K, Tanno M, Mizoguchi J, Otani M, Lee MH, Takasaki T, Kusakabe H, Imagawa DT, Nakai M.
Journal: J Virol Methods (1992): 145

Affinity isolation of active murine erythroleukemia cell chromatin: uniform distribution of ubiquitinated histone H2A between active and inactive fractions
Authors: Dawson BA, Herman T, Haas AL, Lough J.
Journal: J Cell Biochem (1991): 166

Non-radioactive labeling and detection of nucleic acids. IV. Synthesis and properties of digoxigenin-modified 2′-deoxyuridine-5′-triphosphates and a photoactivatable analog of digoxigenin (photodigoxigenin)
Authors: Muhlegger K, Huber E, von der Eltz H, Ruger R, Kessler C.
Journal: Biol Chem Hoppe Seyler (1990): 953

 

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说明书
1mM mFluor dUTP溶液.pdf

1 mM Digoxigenin-11-dUTP溶液 CAS 129030-36-0 货号17012-AAT Bioquest荧光染料

发表于由

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品，欢迎访问AAT Bioquest荧光染料官网了解更多信息。

1 mM Digoxigenin-11-dUTP溶液 CAS 129030-36-0

1 mM Digoxigenin-11-dUTP溶液 CAS 129030-36-0

1 mM Digoxigenin-11-dUTP溶液 CAS 129030-36-0 货号17012 货号 17012 存储条件 在零下15度以下保存, 避免光照
规格 25 nmoles 价格 1944
Ex (nm) Em (nm)
分子量 1132.87 溶剂 Water
产品详细介绍

简要概述

产品基本信息

货号：17012

产品名称：1 mM 地高辛-11-dUTP溶液

CAS：129030-36-0

规格：25nmolse

储存条件：保存在冰箱-15℃干燥

保质期：6个月

 

产品物理化学光谱特性

分子量：1132.87

外观：液体

溶剂：水

激发波长(nm)：N/A

发射波长(nm)：N/A

 

产品介绍

地高辛-11-dUTP可以酶促掺入DNA / cDNA中作为其天然对应物dTTP的替代物。 随后使用与辣根过氧化物酶（HRP），碱性磷酸酶（AP）或荧光染料缀合的洋地黄毒苷 – 抗体检测得到的洋地黄毒苷标记的DNA / cDNA探针.11-原子连接子连接到尿苷的C5位置以最小化 DIG对PCR聚合酶对底物掺入的干扰。 通常1：2地高辛-11-dUTP / dTTP比率用于PCR。

 

参考文献

A visual DNA chip for simultaneous detection of hepatitis B virus, hepatitis C virus and human immunodeficiency virus type-1
Authors: Wen JK, Zhang XE, Cheng Z, Liu H, Zhou YF, Zhang ZP, Yang JH, Deng JY.
Journal: Biosens Bioelectron (2004): 685

Fluorescent DNA hybridization probe preparation using amine modification and reactive dye coupling
Authors: Cox WG, Singer VL.
Journal: Biotechniques (2004): 114

Aminomodified nucleobases: functionalized nucleoside triphosphates applicable for SELEX
Authors: Schoetzau T, Langner J, Moyroud E, Roehl I, Vonhoff S, Klussmann S.
Journal: Bioconjug Chem (2003): 919

Simple method for preparation of fluor/hapten-labeled dUTP
Authors: Nimmakayalu M, Henegariu O, Ward DC, Bray-Ward P.
Journal: Biotechniques (2000): 518

Topology of yeast RNA polymerase II subunits in transcription elongation complexes studied by photoaffinity cross-linking
Authors: Wooddell CI, Burgess RR.
Journal: Biochemistry (2000): 13405

Quantitative analysis of polymerase chain reaction products using biotinylated dUTP incorporation
Authors: Duplaa C, Couffinhal T, Labat L, Moreau C, Lamaziere JM, Bonnet J.
Journal: Anal Biochem (1993): 229

A non-radioisotopic reverse transcriptase assay using biotin-11-deoxyuridinetriphosphate on primer-immobilized microtiter plates
Authors: Urabe T, Sano K, Tanno M, Mizoguchi J, Otani M, Lee MH, Takasaki T, Kusakabe H, Imagawa DT, Nakai M.
Journal: J Virol Methods (1992): 145

Affinity isolation of active murine erythroleukemia cell chromatin: uniform distribution of ubiquitinated histone H2A between active and inactive fractions
Authors: Dawson BA, Herman T, Haas AL, Lough J.
Journal: J Cell Biochem (1991): 166

Non-radioactive labeling and detection of nucleic acids. IV. Synthesis and properties of digoxigenin-modified 2′-deoxyuridine-5′-triphosphates and a photoactivatable analog of digoxigenin (photodigoxigenin)
Authors: Muhlegger K, Huber E, von der Eltz H, Ruger R, Kessler C.
Journal: Biol Chem Hoppe Seyler (1990): 953

说明书
1 mM Digoxigenin-11-dUTP溶液 CAS 129030-36-0.pdf