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5-Propargylamino-3′-azidomethyl-dUTP
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货号 | 17093 | 存储条件 | 在零下15度以下保存, 避免光照 |
| 规格 | 50 nmoles | 价格 | 3108 | |
| Ex (nm) | Em (nm) | |||
| 分子量 | 576.24 | 溶剂 | Water | |
| 产品详细介绍 | ||||
简要概述
产品基本信息
货号：17093
产品名称：5-Propargylamino-3′-azidomethyl-dUTP
规格：50 nmoles
储存条件：-15℃避光防潮
保质期：24个月
产品物理化学光谱特性
分子量：576.24
溶剂：水
产品介绍
5-Propargylamino-3′-azidomethyl-dUTP是制备用于下一代测序（NGS）的荧光缀合物的关键组成部分。 NGS使用与早期Sanger测序相似的链终止方法，但NGS是通过荧光标记的核苷酸类似物作为扩增反应的可逆终止剂来进行的。 NGS依赖于可逆的DNA聚合阻断，而Sanger测序则使用ddNTP不可逆转的DNA聚合阻断。 NGS的另一个不同特征是通过桥式PCR进行体外克隆扩增以增加待测序分子的数量。在此平台上，片段与固定在固体表面上的引物连接，进行原位扩增，生成具有相同分子的DNA簇。在每个循环中，同时添加可逆终止的四个核苷酸，并通过它们互补的聚合酶掺入。通过将3′-OH基团替换为3′-o-叠氮基甲基，这些核苷酸被化学封闭，以防止聚合酶在每个循环中掺入一个以上的核苷酸。掺入核苷酸后，在不同通道中针对不同碱基测量荧光信号。关于下一循环，洗涤未掺入的核苷酸，并用TCEP去除3’端的化学封锁。一旦收集到荧光信号，就会开始一个新的循环，重复此动态过程，直到完成每个片段的测序为止。总之，NGS测序反应分三个步骤进行：核苷酸的添加，成像和通过荧光团裂解的3′-OH再生。
参考文献
Application of Next Generation Sequencing in Laboratory Medicine.
Authors: Zhong, Yiming and Xu, Feng and Wu, Jinhua and Schubert, Jeffrey and Li, Marilyn M
Journal: Annals of laboratory medicine (2021): 25-43
Current scenario of the genetic testing for rare neurological disorders exploiting next generation sequencing.
Authors: Di Resta, Chiara and Pipitone, Giovanni Battista and Carrera, Paola and Ferrari, Maurizio
Journal: Neural regeneration research (2021): 475-481
A New Type of Chronic Wound Infection after Wisdom Tooth Extraction: A Diagnostic Approach with 16S-rRNA Gene Analysis, Next-Generation Sequencing, and Bioinformatics.
Authors: Böttger, Sebastian and Zechel-Gran, Silke and Streckbein, Philipp and Knitschke, Michael and Hain, Torsten and Weigel, Markus and Wilbrand, Jan-Falco and Domann, Eugen and Howaldt, Hans-Peter and Attia, Sameh
Journal: Pathogens (Basel, Switzerland) (2020)
A novel Next-Generation Sequencing-based approach for concurrent detection of mitochondrial DNA copy number and mutation.
Authors: Zhou, Kaixiang and Mo, Qinqin and Guo, Shanshan and Liu, Yang and Yin, Chun and Ji, Xiaoying and Guo, Xu and Xing, Jinliang
Journal: The Journal of molecular diagnostics : JMD (2020)
Amplicon-Based Next-Generation Sequencing for Detection of Fungi in Formalin-Fixed, Paraffin-Embedded Tissues: Correlation with Histopathology and Clinical Applications.
Authors: Larkin, Paige M K and Lawson, Katy L and Contreras, Deisy A and Le, Catherine Q and Trejo, Marisol and Realegeno, Susan and Hilt, Evann E and Chandrasekaran, Sukantha and Garner, Omai B and Fishbein, Gregory A and Yang, Shangxin
Journal: The Journal of molecular diagnostics : JMD (2020): 1287-1293
Analytical performance evaluation of a commercial next generation sequencing liquid biopsy platform using plasma ctDNA, reference standards, and synthetic serial dilution samples derived from normal plasma.
Authors: Verma, Suman and Moore, Mathew W and Ringler, Rebecca and Ghosal, Abhisek and Horvath, Kyle and Naef, Theodore and Anvari, Sheri and Cotter, Philip D and Gunn, Shelly
Journal: BMC cancer (2020): 945
Author Correction: Ultrasensitive amplicon barcoding for next-generation sequencing facilitating sequence error and amplification-bias correction.
Authors: Ahmed, Ibrahim and Tucci, Felicia A and Aflalo, Aure and Smith, Kenneth G C and Bashford-Rogers, Rachael J M
Journal: Scientific reports (2020): 17010
Automation of Amplicon-Based Library Preparation for Next-Generation Sequencing by Centrifugal Microfluidics.
Authors: Hess, Jacob Friedrich and Kotrová, Michaela and Calabrese, Silvia and Darzentas, Nikos and Hutzenlaub, Tobias and Zengerle, Roland and Brüggemann, Monika and Paust, Nils
Journal: Analytical chemistry (2020): 12833-12841
Characterization of the novel HLA-B*15:474 allele by next-generation sequencing.
Authors: Genebrier, Steve and Elsermans, Vincent and Texeraud, Emeric and Bertrand, Gerald and Renac, Virginie
Journal: HLA (2020)
Correction to: Malignant struma ovarii: next-generation sequencing of six cases revealed Nras, Braf, and Jak3 mutations.
Authors: Poli, Roberta and Scatolini, Maria and Grosso, Enrico and Maletta, Francesca and Gallo, Marco and Liscia, Daniele and Nelva, Anna and Cesario, Flora and Forte, Giuseppe and Metovic, Jasna and Volante, Marco and Arvat, Emanuela and Papotti, Mauro
Journal: Endocrine (2020)
