trFluor™Eu DBCO 货号1446-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。

trFluor™Eu DBCO

trFluor™Eu DBCO

trFluor™Eu DBCO    货号1446 货号 1446 存储条件 在零下15度以下保存, 避免光照
规格 100 ug 价格 3732
Ex (nm) 346 Em (nm) 617
分子量 1765.72 溶剂 DMSO
产品详细介绍

简要概述

产品基本信息

货号:1446

产品名称:trFluor™Eu DBCO

规格:100ug

储存条件:-15℃避光防潮

保质期:24个月

 

产品物理化学光谱特性

分子量:1765.72

外观:固体

溶剂:DMSO

激发波长(nm):346

发射波长(nm):617

 

产品介绍

存在于细胞,血清或其他生物流体中的许多生物化合物都是天然荧光的,因此,由于要测定的生物分子的自发荧光引起的高背景,使用常规的快速荧光团会严重限制测定灵敏度。长寿命荧光团与时间分辨检测(激发和发射检测之间的延迟)的结合使用可最大程度地减少即时荧光干扰。我们的trFluor™Eu探针可用于需要高灵敏度测定的时间分辨荧光(TRF)。与诸如Alexa Fluor或花青染料等更传统的荧光团相比,这些trFluor™Eu探针具有较大的斯托克斯位移和极长的发射半衰期。与其他TRF化合物相比,我们的trFluor™Eu探针具有相对较高的稳定性,高发射率和与生物分子连接的能力。此外,当与生物聚合物(例如抗体)偶联时,我们的trFluor™Eu探针对荧光猝灭不敏感。在无铜条件下,trFluor™Eu DBCO可以容易地单击成含有叠氮基的分子。金畔生物是AAT Bioquest的中国代理商,为您提供最优质的trFluor™I DBCO。 

点击查看光谱

 

参考文献

Comparative Analysis of High-Throughput Assays of Family-1 Plant Glycosyltransferases.
Authors: McGraphery, Kate and Schwab, Wilfried
Journal: International journal of molecular sciences (2020)

Discovery and Characterization of Peptide Inhibitors for Calcium and Integrin Binding Protein 1.
Authors: Puhl, Ana C and Bogart, Jonathan W and Haberman, Victoria A and Larson, Jacob E and Godoy, Andre S and Norris-Drouin, Jacqueline L and Cholensky, Stephanie H and Leisner, Tina M and Frye, Stephen V and Parise, Leslie V and Bowers, Albert A and Pearce, Kenneth H
Journal: ACS chemical biology (2020)

Integrating Ligand and Target-Driven Based Virtual Screening Approaches With in vitro Human Cell Line Models and Time-Resolved Fluorescence Resonance Energy Transfer Assay to Identify Novel Hit Compounds Against BCL-2.
Authors: Tutumlu, Gurbet and Dogan, Berna and Avsar, Timucin and Orhan, Muge Didem and Calis, Seyma and Durdagi, Serdar
Journal: Frontiers in chemistry (2020): 167

Revisit ligand-receptor interaction at the human vasopressin V2 receptor: A kinetic perspective.
Authors: Liu, Chunji and Xia, Leyi and Fu, Kequan and Cao, Xudong and Yan, Wenzhong and Cheng, Jianjun and Roux, Thomas and Peletier, Lambertus A and Yin, Xiaoxing and Guo, Dong
Journal: European journal of pharmacology (2020): 173157

A General TR-FRET Assay Platform for High-Throughput Screening and Characterizing Inhibitors of Methyl-Lysine Reader Proteins.
Authors: Rectenwald, Justin M and Hardy, P Brian and Norris-Drouin, Jacqueline L and Cholensky, Stephanie H and James, Lindsey I and Frye, Stephen V and Pearce, Kenneth H
Journal: SLAS discovery : advancing life sciences R & D (2019): 693-700

A high-throughput screen of pharmacologically active compounds for inhibitors of UHRF1 reveals epigenetic activity of anthracycline derivative chemotherapeutic drugs.
Authors: Giovinazzo, Hugh and Walker, David and Wyhs, Nicolas and Liu, Jianyong and Esopi, David M and Vaghasia, Ajay M and Jain, Yash and Bhamidipati, Akshay and Zhou, Jianya and Nelson, William G and Yegnasubramanian, Srinivasan
Journal: Oncotarget (2019): 3040-3050

Development of a High-Throughput Cul3-Keap1 Time-Resolved Fluorescence Resonance Energy Transfer (TR-FRET) Assay for Identifying Nrf2 Activators.
Authors: Poore, Derek D and Hofmann, Glenn and Wolfe, Lawrence A and Qi, Hongwei and Jiang, Ming and Fischer, Michael and Wu, Zining and Sweitzer, Thomas D and Chakravorty, Subhas and Donovan, Brian and Li, Hu
Journal: SLAS discovery : advancing life sciences R & D (2019): 175-189

LFRET, a novel rapid assay for anti-tissue transglutaminase antibody detection.
Authors: Rusanen, Juuso and Toivonen, Anne and Hepojoki, Jussi and Hepojoki, Satu and Arikoski, Pekka and Heikkinen, Markku and Vaarala, Outi and Ilonen, Jorma and Hedman, Klaus
Journal: PloS one (2019): e0225851

Quantitative high-throughput screening assays for the discovery and development of SIRPα-CD47 interaction inhibitors.
Authors: Miller, Thomas W and Amason, Joshua D and Garcin, Elsa D and Lamy, Laurence and Dranchak, Patricia K and Macarthur, Ryan and Braisted, John and Rubin, Jeffrey S and Burgess, Teresa L and Farrell, Catherine L and Roberts, David D and Inglese, James
Journal: PloS one (2019): e0218897

Development of a Broadly Applicable Assay for Measurement of Glycan-Directed Enzymatic Activity.
Authors: Bresciani, Alberto and Cecchetti, Ottavia and Missineo, Antonino and Pacifici, Pier Giorgio and Tomei, Licia and Rodems, Steven
Journal: SLAS discovery : advancing life sciences R & D (2018): 941-950

说明书
trFluor™Eu DBCO.pdf

trFluor Eu 驴抗山羊IgG(H + L)*交叉吸附* 货号62000-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。

trFluor Eu 驴抗山羊IgG(H + L)*交叉吸附*

trFluor Eu 驴抗山羊IgG(H + L)*交叉吸附*

trFluor Eu 驴抗山羊IgG(H + L)*交叉吸附*     货号62000 货号 62000 存储条件 在零下15度以下保存, 避免光照
规格 50 μg 价格 3924
Ex (nm) 346 Em (nm) 617
分子量 ~150000 溶剂 Water
产品详细介绍

简要概述

产品基本信息

货号:62000

产品名称:trFluor Eu 驴抗山羊IgG(H + L)

规格:50ug

储存条件:-15℃避光防潮

保质期:24个月

 

产品物理化学光谱特性

分子量:~150000

溶剂:水

激发波长(nm):346

发射波长(nm):617

 

产品介绍

存在于细胞,血清或其他生物流体中的许多生物化合物都是天然荧光的,因此,由于要测定的生物分子的自发荧光引起的高背景,使用常规的快速荧光团会严重限制测定灵敏度。长寿命荧光团与时间分辨检测(激发和发射检测之间的延迟)结合使用,可最大程度地减少即时荧光干扰。我们的trFluor Eu探针可用于需要高灵敏度的测定的时间分辨荧光(TRF)。与更传统的荧光团(例如Alexa Fluor或花青染料)相比,trFluor Eu探针具有较大的斯托克斯位移和极长的发射半衰期。与其他TRF化合物相比,我们的trFluor Eu探针具有相对较高的稳定性,高发射率和与生物分子连接的能力。当与一抗结合使用时,这种trFluor Eu驴抗山羊IgG(H + L)缀合物通常用作间接免疫荧光染色的第二步试剂。金畔生物是AAT Bioquest的中国代理商,为您提供最优质的trFluor Eu 驴抗山羊IgG(H + L)。 

点击查看光谱

 

图示

trFluor Eu 驴抗山羊IgG(H + L)*交叉吸附*     货号62000

图1.时间分辨荧光能量转移(TR-FRET)是时间分辨荧光法(TRF)与Förster共振能量转移(FRET)的实际结合,为药物发现研究人员提供了强大的工具。 TR-FRET将TRF的低背景方面与FRET的均相测定形式结合在一起。除更高的通量和更少的假阳性/假阴性结果外,所得测定法还提高了灵活性,可靠性和灵敏性。 FRET涉及两个荧光团,一个供体(例如trFluor Eu和trFluor Tb)和一个受体。如果能量源(例如闪光灯或激光器)对供体的激发,则如果两者在给定的彼此邻近范围内,则将能量转移至受体。受体继而以其特征波长发射光。该技术的FRET方面受多种因素驱动,包括光谱重叠和所涉及的荧光团的邻近性,其中仅当施主与受主之间的距离足够小时才发生能量转移。实际上,FRET系统的特征在于Förster半径(R0):FRET效率为50%时荧光团之间的距离。对于许多FRET配对,R0介于20至90Å之间,具体取决于所使用的受体和检测中荧光团的空间排列。通过测量这种能量转移,可以通过将每个配偶与荧光标记偶联并检测能量转移的水平来评估生物分子之间的相互作用。可以检测受体发射作为能量转移的量度,而无需将结合的与未结合的测定成分分开(例如过滤或洗涤步骤),从而减少了测定时间和成本。

 

参考文献

Development of a time-resolved fluorescence resonance energy transfer assay for cyclin-dependent kinase 4 and identification of its ATP-noncompetitive inhibitors
Authors: Lo MC, Ngo R, Dai K, Li C, Liang L, Lee J, Emkey R, Eksterowicz J, Ventura M, Young SW, Xiao SH.
Journal: Anal Biochem (2012): 368

Time-Resolved Fluorescence Resonance Energy Transfer as a Versatile Tool in the Development of Homogeneous Cellular Kinase Assays
Authors: Saville L, Spais C, Mason JL, Albom MS, Murthy S, Meyer SL, Ator MA, Angeles TS, Husten J.
Journal: Assay Drug Dev Technol. (2012)

A homogeneous single-label time-resolved fluorescence cAMP assay
Authors: Martikkala E, Rozw and owicz-Jansen A, Hanninen P, Petaja-Repo U, Harma H.
Journal: J Biomol Screen (2011): 356

Homogeneous time-resolved fluorescence-based assay to screen for ligands targeting the growth hormone secretagogue receptor type 1a
Authors: Leyris JP, Roux T, Trinquet E, Verdie P, Fehrentz JA, Oueslati N, Douzon S, Bourrier E, Lamarque L, Gagne D, Galleyr and JC, M’Kadmi C, Martinez J, Mary S, Baneres JL, Marie J.
Journal: Anal Biochem (2011): 253

Oligomerization of the serotonin(1A) receptor in live cells: a time-resolved fluorescence anisotropy approach
Authors: Paila YD, Kombrabail M, Krishnamoorthy G, Chattopadhyay A.
Journal: J Phys Chem B (2011): 11439

Time-resolved fluorescence resonance energy transfer (TR-FRET) to analyze the disruption of EGFR/HER2 dimers: a new method to evaluate the efficiency of targeted therapy using monoclonal antibodies
Authors: Gaborit N, Larbouret C, Vallaghe J, Peyrusson F, Bascoul-Mollevi C, Crapez E, Azria D, Chardes T, Poul MA, Mathis G, Bazin H, Pelegrin A.
Journal: J Biol Chem (2011): 11337

A time-resolved fluorescence-resonance energy transfer assay for identifying inhibitors of hepatitis C virus core dimerization
Authors: Kota S, Scampavia L, Spicer T, Beeler AB, Takahashi V, Snyder JK, Porco JA, Hodder P, Strosberg AD.
Journal: Assay Drug Dev Technol (2010): 96

Ligand regulation of the quaternary organization of cell surface M3 muscarinic acetylcholine receptors analyzed by fluorescence resonance energy transfer (FRET) imaging and homogeneous time-resolved FRET
Authors: Alvarez-Curto E, Ward RJ, Pediani JD, Milligan G.
Journal: J Biol Chem (2010): 23318

Steady-state and time-resolved fluorescence quenching with transition metal ions as short-distance probes for protein conformation
Authors: Posokhov YO, Kyrychenko A, Ladokhin AS.
Journal: Anal Biochem (2010): 284

Time-resolved FRET fluorescence spectroscopy of visible fluorescent protein pairs
Authors: Visser AJ, Laptenok SP, Visser NV, van Hoek A, Birch DJ, Brochon JC, Borst JW.
Journal: Eur Biophys J (2010): 241

说明书
trFluor Eu 驴抗山羊IgG(H + L)*交叉吸附* .pdf

trFluor Eu羊抗兔免疫球蛋白(H+L)*交叉亲和 降低干扰* 货号16847-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。

trFluor Eu羊抗兔免疫球蛋白(H+L)*交叉亲和 降低干扰*

trFluor Eu羊抗兔免疫球蛋白(H+L)*交叉亲和 降低干扰*

trFluor Eu羊抗兔免疫球蛋白(H+L)*交叉亲和 降低干扰*    货号16847 货号 16847 存储条件 在零下15度以下保存, 避免光照
规格 1 mg 价格 39060
Ex (nm) 346 Em (nm) 617
分子量 ~150000 溶剂 Water
产品详细介绍

简要概述

产品基本信息

货号:16847

产品名称:trFluor Eu羊抗兔免疫球蛋白(H+L)*交叉亲和 降低干扰*

规格:1mg

储存条件:-15℃避光防潮

保质期:24个月

 

产品物理化学光谱特性

分子量:N/A

溶剂:水

激发波长(nm):346

发射波长(nm):617

 

产品介绍

trFluor Eu羊抗兔免疫球蛋白(H+L)是美国AAT Bioquest生产的荧光标记二抗,存在于细胞,血清或其他生物流体中的许多生物化合物都是天然荧光的,因此,由于要测定的生物分子的自发荧光引起的高背景,使用常规的快速荧光团会严重限制测定灵敏度。长寿命荧光团与时间分辨检测(激发和发射检测之间的延迟)结合使用,可最大程度地减少即时荧光干扰。我们的trFluor Eu探针可用于需要高灵敏度的测定的时间分辨荧光(TRF)。与更传统的荧光团(例如Alexa Fluor或花青染料)相比,trFluor Eu探针具有较大的斯托克斯位移和极长的发射半衰期。与其他TRF化合物相比,我们的trFluor Eu探针具有相对较高的稳定性,高发射率和与生物分子连接的能力。当与一抗结合使用时,这种trFluor Eu山羊抗兔IgG(H + L)缀合物通常用作间接免疫荧光染色的第二步试剂。金畔生物是AAT Bioquest的中国代理商,为您提供最优质的trFluor Eu羊抗兔免疫球蛋白(H+L)。 

点击查看光谱

 

参考文献

Development of a time-resolved fluorescence resonance energy transfer assay for cyclin-dependent kinase 4 and identification of its ATP-noncompetitive inhibitors
Authors: Lo MC, Ngo R, Dai K, Li C, Liang L, Lee J, Emkey R, Eksterowicz J, Ventura M, Young SW, Xiao SH.
Journal: Anal Biochem (2012): 368

Time-Resolved Fluorescence Resonance Energy Transfer as a Versatile Tool in the Development of Homogeneous Cellular Kinase Assays
Authors: Saville L, Spais C, Mason JL, Albom MS, Murthy S, Meyer SL, Ator MA, Angeles TS, Husten J.
Journal: Assay Drug Dev Technol. (2012)

A homogeneous single-label time-resolved fluorescence cAMP assay
Authors: Martikkala E, Rozwandowicz-Jansen A, Hanninen P, Petaja-Repo U, Harma H.
Journal: J Biomol Screen (2011): 356

Homogeneous time-resolved fluorescence-based assay to screen for ligands targeting the growth hormone secretagogue receptor type 1a
Authors: Leyris JP, Roux T, Trinquet E, Verdie P, Fehrentz JA, Oueslati N, Douzon S, Bourrier E, Lamarque L, Gagne D, Galleyrand JC, M’Kadmi C, Martinez J, Mary S, Baneres JL, Marie J.
Journal: Anal Biochem (2011): 253

Oligomerization of the serotonin(1A) receptor in live cells: a time-resolved fluorescence anisotropy approach
Authors: Paila YD, Kombrabail M, Krishnamoorthy G, Chattopadhyay A.
Journal: J Phys Chem B (2011): 11439

Time-resolved fluorescence resonance energy transfer (TR-FRET) to analyze the disruption of EGFR/HER2 dimers: a new method to evaluate the efficiency of targeted therapy using monoclonal antibodies
Authors: Gaborit N, Larbouret C, Vallaghe J, Peyrusson F, Bascoul-Mollevi C, Crapez E, Azria D, Chardes T, Poul MA, Mathis G, Bazin H, Pelegrin A.
Journal: J Biol Chem (2011): 11337

A time-resolved fluorescence-resonance energy transfer assay for identifying inhibitors of hepatitis C virus core dimerization
Authors: Kota S, Scampavia L, Spicer T, Beeler AB, Takahashi V, Snyder JK, Porco JA, Hodder P, Strosberg AD.
Journal: Assay Drug Dev Technol (2010): 96

Ligand regulation of the quaternary organization of cell surface M3 muscarinic acetylcholine receptors analyzed by fluorescence resonance energy transfer (FRET) imaging and homogeneous time-resolved FRET
Authors: Alvarez-Curto E, Ward RJ, Pediani JD, Milligan G.
Journal: J Biol Chem (2010): 23318

Steady-state and time-resolved fluorescence quenching with transition metal ions as short-distance probes for protein conformation
Authors: Posokhov YO, Kyrychenko A, Ladokhin AS.
Journal: Anal Biochem (2010): 284

Time-resolved FRET fluorescence spectroscopy of visible fluorescent protein pairs
Authors: Visser AJ, Laptenok SP, Visser NV, van Hoek A, Birch DJ, Brochon JC, Borst JW.
Journal: Eur Biophys J (2010): 241

 

相关产品

产品名称 货号
trFluor Eu羊抗鼠免疫球蛋白(H+L)*交叉亲和 降低干扰* Cat#16598
iFluor 647羊抗兔免疫球蛋白(H+L)*交叉亲和 降低干扰* Cat#16710
iFluor 555羊抗兔免疫球蛋白(H+L)*交叉亲和 降低干扰* Cat#16690

说明书
trFluor Eu羊抗兔免疫球蛋白(H+L)*交叉亲和 降低干扰*.pdf

trFluor Eu羊抗鼠免疫球蛋白(H+L)*交叉亲和 降低干扰* 货号16598-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。

trFluor Eu羊抗鼠免疫球蛋白(H+L)*交叉亲和 降低干扰*

trFluor Eu羊抗鼠免疫球蛋白(H+L)*交叉亲和 降低干扰*

trFluor Eu羊抗鼠免疫球蛋白(H+L)*交叉亲和 降低干扰*    货号16598 货号 16598 存储条件 在零下15度以下保存, 避免光照
规格 100 ug 价格 5244
Ex (nm) 346 Em (nm) 617
分子量 ~150000 溶剂 Water
产品详细介绍

简要概述

产品基本信息

货号:16598

产品名称:trFluor Eu羊抗鼠免疫球蛋白(H+L)*交叉亲和 降低干扰*

规格:100 ug

储存条件:-15℃避光防潮

保质期:24个月

 

产品物理化学光谱特性

分子量:N/A

溶剂:水

激发波长(nm):346

发射波长(nm):617

 

产品介绍

trFluor Eu山羊抗小鼠IgG(H + L)是美国AAT Bioquest生产的荧光标记二抗,存在于细胞,血清或其他生物流体中的许多生物化合物都是天然荧光的,因此,由于要测定的生物分子的自发荧光引起的高背景,使用常规的快速荧光团会严重限制测定灵敏度。长寿命荧光团与时间分辨检测(激发和发射检测之间的延迟)结合使用,可最大程度地减少即时荧光干扰。我们的trFluor Eu探针可用于需要高灵敏度的测定的时间分辨荧光(TRF)。与更传统的荧光团(例如Alexa Fluor或花青染料)相比,trFluor Eu探针具有较大的斯托克斯位移和极长的发射半衰期。与其他TRF化合物相比,我们的trFluor Eu探针具有相对较高的稳定性,高发射率和与生物分子连接的能力。当与一抗结合使用时,这种trFluor Eu山羊抗小鼠IgG(H + L)缀合物通常用作间接免疫荧光染色的第二步试剂。金畔生物是AAT Bioquest的中国代理商,为您提供最优质的trFluor Eu山羊抗小鼠IgG(H + L)。 

点击查看光谱

 

参考文献

Development of a time-resolved fluorescence resonance energy transfer assay for cyclin-dependent kinase 4 and identification of its ATP-noncompetitive inhibitors
Authors: Lo MC, Ngo R, Dai K, Li C, Liang L, Lee J, Emkey R, Eksterowicz J, Ventura M, Young SW, Xiao SH.
Journal: Anal Biochem (2012): 368

Time-Resolved Fluorescence Resonance Energy Transfer as a Versatile Tool in the Development of Homogeneous Cellular Kinase Assays
Authors: Saville L, Spais C, Mason JL, Albom MS, Murthy S, Meyer SL, Ator MA, Angeles TS, Husten J.
Journal: Assay Drug Dev Technol. (2012)

A homogeneous single-label time-resolved fluorescence cAMP assay
Authors: Martikkala E, Rozwandowicz-Jansen A, Hanninen P, Petaja-Repo U, Harma H.
Journal: J Biomol Screen (2011): 356

Homogeneous time-resolved fluorescence-based assay to screen for ligands targeting the growth hormone secretagogue receptor type 1a
Authors: Leyris JP, Roux T, Trinquet E, Verdie P, Fehrentz JA, Oueslati N, Douzon S, Bourrier E, Lamarque L, Gagne D, Galleyrand JC, M’Kadmi C, Martinez J, Mary S, Baneres JL, Marie J.
Journal: Anal Biochem (2011): 253

Oligomerization of the serotonin(1A) receptor in live cells: a time-resolved fluorescence anisotropy approach
Authors: Paila YD, Kombrabail M, Krishnamoorthy G, Chattopadhyay A.
Journal: J Phys Chem B (2011): 11439

Time-resolved fluorescence resonance energy transfer (TR-FRET) to analyze the disruption of EGFR/HER2 dimers: a new method to evaluate the efficiency of targeted therapy using monoclonal antibodies
Authors: Gaborit N, Larbouret C, Vallaghe J, Peyrusson F, Bascoul-Mollevi C, Crapez E, Azria D, Chardes T, Poul MA, Mathis G, Bazin H, Pelegrin A.
Journal: J Biol Chem (2011): 11337

A time-resolved fluorescence-resonance energy transfer assay for identifying inhibitors of hepatitis C virus core dimerization
Authors: Kota S, Scampavia L, Spicer T, Beeler AB, Takahashi V, Snyder JK, Porco JA, Hodder P, Strosberg AD.
Journal: Assay Drug Dev Technol (2010): 96

Ligand regulation of the quaternary organization of cell surface M3 muscarinic acetylcholine receptors analyzed by fluorescence resonance energy transfer (FRET) imaging and homogeneous time-resolved FRET
Authors: Alvarez-Curto E, Ward RJ, Pediani JD, Milligan G.
Journal: J Biol Chem (2010): 23318

Steady-state and time-resolved fluorescence quenching with transition metal ions as short-distance probes for protein conformation
Authors: Posokhov YO, Kyrychenko A, Ladokhin AS.
Journal: Anal Biochem (2010): 284

Time-resolved FRET fluorescence spectroscopy of visible fluorescent protein pairs
Authors: Visser AJ, Laptenok SP, Visser NV, van Hoek A, Birch DJ, Brochon JC, Borst JW.
Journal: Eur Biophys J (2010): 241

 

相关产品

产品名称 货号
trFluor Eu羊抗兔免疫球蛋白(H+L)*交叉亲和 降低干扰* Cat#16725
iFluor 555羊抗鼠免疫球蛋白(H+L)*交叉亲和 降低干扰* Cat#16540
iFluor 647羊抗鼠免疫球蛋白(H+L) *交叉亲和 降低干扰* Cat#16562

说明书
trFluor Eu羊抗鼠免疫球蛋白(H+L)*交叉亲和 降低干扰*.pdf

trFluor Eu羊抗鼠免疫球蛋白(H+L) 货号16755-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。

trFluor Eu羊抗鼠免疫球蛋白(H+L)

trFluor Eu羊抗鼠免疫球蛋白(H+L)

trFluor Eu羊抗鼠免疫球蛋白(H+L)    货号16755 货号 16755 存储条件 在零下15度以下保存, 避免光照
规格 1 mg 价格 25836
Ex (nm) 346 Em (nm) 617
分子量 ~150000 溶剂 Water
产品详细介绍

简要概述

产品基本信息

货号:16755

产品名称:trFluor Eu羊抗鼠免疫球蛋白(H+L)

规格:1mg

储存条件:-15℃避光防潮

保质期:24个月

 

产品物理化学光谱特性

分子量:N/A

溶剂:水

激发波长(nm):346

发射波长(nm):617

 

产品介绍

trFluor Eu羊抗鼠免疫球蛋白(H+L)是美国AAT Bioquest生产的荧光标记二抗,许多生物分子自身就具有荧光,它们广泛的存在于生物器官里,如细胞、浆液和其他生物制品中。因此传统的敏感荧光素存在严重的局限性和不足,在进行生物监测分析时,生物分子的自发荧光形成很高的背景干扰。解决的方法是用长寿命的荧光素轭合物它具有高的时间分辨率(延长激发波谱和发射波普的检测)最大程度的减少背景荧光干扰。我们的 trFluor Eu荧光探针具有高灵敏度的能够做到时间分辨检测。在和传统的荧光探针相比(例如Alexa的Fluro和花箐染料), trFluor Eu 荧光探针具有较大的斯托克斯位移和极其长的发射光谱半衰期。同另外的TRF化合物相比我们的 trFluor Eu荧光探针具有较高的稳定性和很高的量子产率,而且可以和生物分子相连接。这一 trFluor Eu  山羊-老鼠 IgG (H+L) 轭合物在连接生物素酰化的第一抗体时,通常用作第二步简接免疫荧光试剂。在以生物素莲亲和素为基础的生物学流式测试或者分析方面他是一个非常有用的工具。金畔生物是AAT Bioquest的中国代理商,为您提供最优质的trFluor Eu羊抗鼠免疫球蛋白(H+L)。 

点击查看光谱

 

参考文献

Development of a time-resolved fluorescence resonance energy transfer assay for cyclin-dependent kinase 4 and identification of its ATP-noncompetitive inhibitors
Authors: Lo MC, Ngo R, Dai K, Li C, Liang L, Lee J, Emkey R, Eksterowicz J, Ventura M, Young SW, Xiao SH.
Journal: Anal Biochem (2012): 368

Time-Resolved Fluorescence Resonance Energy Transfer as a Versatile Tool in the Development of Homogeneous Cellular Kinase Assays
Authors: Saville L, Spais C, Mason JL, Albom MS, Murthy S, Meyer SL, Ator MA, Angeles TS, Husten J.
Journal: Assay Drug Dev Technol. (2012)

A homogeneous single-label time-resolved fluorescence cAMP assay
Authors: Martikkala E, Rozwandowicz-Jansen A, Hanninen P, Petaja-Repo U, Harma H.
Journal: J Biomol Screen (2011): 356

Homogeneous time-resolved fluorescence-based assay to screen for ligands targeting the growth hormone secretagogue receptor type 1a
Authors: Leyris JP, Roux T, Trinquet E, Verdie P, Fehrentz JA, Oueslati N, Douzon S, Bourrier E, Lamarque L, Gagne D, Galleyrand JC, M’Kadmi C, Martinez J, Mary S, Baneres JL, Marie J.
Journal: Anal Biochem (2011): 253

Oligomerization of the serotonin(1A) receptor in live cells: a time-resolved fluorescence anisotropy approach
Authors: Paila YD, Kombrabail M, Krishnamoorthy G, Chattopadhyay A.
Journal: J Phys Chem B (2011): 11439

Time-resolved fluorescence resonance energy transfer (TR-FRET) to analyze the disruption of EGFR/HER2 dimers: a new method to evaluate the efficiency of targeted therapy using monoclonal antibodies
Authors: Gaborit N, Larbouret C, Vallaghe J, Peyrusson F, Bascoul-Mollevi C, Crapez E, Azria D, Chardes T, Poul MA, Mathis G, Bazin H, Pelegrin A.
Journal: J Biol Chem (2011): 11337

A time-resolved fluorescence-resonance energy transfer assay for identifying inhibitors of hepatitis C virus core dimerization
Authors: Kota S, Scampavia L, Spicer T, Beeler AB, Takahashi V, Snyder JK, Porco JA, Hodder P, Strosberg AD.
Journal: Assay Drug Dev Technol (2010): 96

Ligand regulation of the quaternary organization of cell surface M3 muscarinic acetylcholine receptors analyzed by fluorescence resonance energy transfer (FRET) imaging and homogeneous time-resolved FRET
Authors: Alvarez-Curto E, Ward RJ, Pediani JD, Milligan G.
Journal: J Biol Chem (2010): 23318

Steady-state and time-resolved fluorescence quenching with transition metal ions as short-distance probes for protein conformation
Authors: Posokhov YO, Kyrychenko A, Ladokhin AS.
Journal: Anal Biochem (2010): 284

Time-resolved FRET fluorescence spectroscopy of visible fluorescent protein pairs
Authors: Visser AJ, Laptenok SP, Visser NV, van Hoek A, Birch DJ, Brochon JC, Borst JW.
Journal: Eur Biophys J (2010): 241

 

相关产品

产品名称 货号
trFluor Tb羊抗鼠免疫球蛋白(H+L) Cat#16519
trFluor Eu羊抗兔免疫球蛋白(H+L) Cat#16668
FITC羊抗鼠免疫球蛋白(H+L) Cat#16852

说明书
trFluor Eu羊抗鼠免疫球蛋白(H+L).pdf