trFluor™Eu DBCO 货号1446-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。

trFluor™Eu DBCO

trFluor™Eu DBCO

trFluor™Eu DBCO    货号1446 货号 1446 存储条件 在零下15度以下保存, 避免光照
规格 100 ug 价格 3732
Ex (nm) 346 Em (nm) 617
分子量 1765.72 溶剂 DMSO
产品详细介绍

简要概述

产品基本信息

货号:1446

产品名称:trFluor™Eu DBCO

规格:100ug

储存条件:-15℃避光防潮

保质期:24个月

 

产品物理化学光谱特性

分子量:1765.72

外观:固体

溶剂:DMSO

激发波长(nm):346

发射波长(nm):617

 

产品介绍

存在于细胞,血清或其他生物流体中的许多生物化合物都是天然荧光的,因此,由于要测定的生物分子的自发荧光引起的高背景,使用常规的快速荧光团会严重限制测定灵敏度。长寿命荧光团与时间分辨检测(激发和发射检测之间的延迟)的结合使用可最大程度地减少即时荧光干扰。我们的trFluor™Eu探针可用于需要高灵敏度测定的时间分辨荧光(TRF)。与诸如Alexa Fluor或花青染料等更传统的荧光团相比,这些trFluor™Eu探针具有较大的斯托克斯位移和极长的发射半衰期。与其他TRF化合物相比,我们的trFluor™Eu探针具有相对较高的稳定性,高发射率和与生物分子连接的能力。此外,当与生物聚合物(例如抗体)偶联时,我们的trFluor™Eu探针对荧光猝灭不敏感。在无铜条件下,trFluor™Eu DBCO可以容易地单击成含有叠氮基的分子。金畔生物是AAT Bioquest的中国代理商,为您提供最优质的trFluor™I DBCO。 

点击查看光谱

 

参考文献

Comparative Analysis of High-Throughput Assays of Family-1 Plant Glycosyltransferases.
Authors: McGraphery, Kate and Schwab, Wilfried
Journal: International journal of molecular sciences (2020)

Discovery and Characterization of Peptide Inhibitors for Calcium and Integrin Binding Protein 1.
Authors: Puhl, Ana C and Bogart, Jonathan W and Haberman, Victoria A and Larson, Jacob E and Godoy, Andre S and Norris-Drouin, Jacqueline L and Cholensky, Stephanie H and Leisner, Tina M and Frye, Stephen V and Parise, Leslie V and Bowers, Albert A and Pearce, Kenneth H
Journal: ACS chemical biology (2020)

Integrating Ligand and Target-Driven Based Virtual Screening Approaches With in vitro Human Cell Line Models and Time-Resolved Fluorescence Resonance Energy Transfer Assay to Identify Novel Hit Compounds Against BCL-2.
Authors: Tutumlu, Gurbet and Dogan, Berna and Avsar, Timucin and Orhan, Muge Didem and Calis, Seyma and Durdagi, Serdar
Journal: Frontiers in chemistry (2020): 167

Revisit ligand-receptor interaction at the human vasopressin V2 receptor: A kinetic perspective.
Authors: Liu, Chunji and Xia, Leyi and Fu, Kequan and Cao, Xudong and Yan, Wenzhong and Cheng, Jianjun and Roux, Thomas and Peletier, Lambertus A and Yin, Xiaoxing and Guo, Dong
Journal: European journal of pharmacology (2020): 173157

A General TR-FRET Assay Platform for High-Throughput Screening and Characterizing Inhibitors of Methyl-Lysine Reader Proteins.
Authors: Rectenwald, Justin M and Hardy, P Brian and Norris-Drouin, Jacqueline L and Cholensky, Stephanie H and James, Lindsey I and Frye, Stephen V and Pearce, Kenneth H
Journal: SLAS discovery : advancing life sciences R & D (2019): 693-700

A high-throughput screen of pharmacologically active compounds for inhibitors of UHRF1 reveals epigenetic activity of anthracycline derivative chemotherapeutic drugs.
Authors: Giovinazzo, Hugh and Walker, David and Wyhs, Nicolas and Liu, Jianyong and Esopi, David M and Vaghasia, Ajay M and Jain, Yash and Bhamidipati, Akshay and Zhou, Jianya and Nelson, William G and Yegnasubramanian, Srinivasan
Journal: Oncotarget (2019): 3040-3050

Development of a High-Throughput Cul3-Keap1 Time-Resolved Fluorescence Resonance Energy Transfer (TR-FRET) Assay for Identifying Nrf2 Activators.
Authors: Poore, Derek D and Hofmann, Glenn and Wolfe, Lawrence A and Qi, Hongwei and Jiang, Ming and Fischer, Michael and Wu, Zining and Sweitzer, Thomas D and Chakravorty, Subhas and Donovan, Brian and Li, Hu
Journal: SLAS discovery : advancing life sciences R & D (2019): 175-189

LFRET, a novel rapid assay for anti-tissue transglutaminase antibody detection.
Authors: Rusanen, Juuso and Toivonen, Anne and Hepojoki, Jussi and Hepojoki, Satu and Arikoski, Pekka and Heikkinen, Markku and Vaarala, Outi and Ilonen, Jorma and Hedman, Klaus
Journal: PloS one (2019): e0225851

Quantitative high-throughput screening assays for the discovery and development of SIRPα-CD47 interaction inhibitors.
Authors: Miller, Thomas W and Amason, Joshua D and Garcin, Elsa D and Lamy, Laurence and Dranchak, Patricia K and Macarthur, Ryan and Braisted, John and Rubin, Jeffrey S and Burgess, Teresa L and Farrell, Catherine L and Roberts, David D and Inglese, James
Journal: PloS one (2019): e0218897

Development of a Broadly Applicable Assay for Measurement of Glycan-Directed Enzymatic Activity.
Authors: Bresciani, Alberto and Cecchetti, Ottavia and Missineo, Antonino and Pacifici, Pier Giorgio and Tomei, Licia and Rodems, Steven
Journal: SLAS discovery : advancing life sciences R & D (2018): 941-950

说明书
trFluor™Eu DBCO.pdf

花菁素DBCO-Cy3 CAS 1782950-79-1 货号920-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。

花菁素DBCO-Cy3 CAS 1782950-79-1

花菁素DBCO-Cy3 CAS 1782950-79-1

花菁素DBCO-Cy3 CAS 1782950-79-1    货号920 货号 920 存储条件 在零下15度以下保存, 避免光照
规格 1 mg 价格 1272
Ex (nm) 555 Em (nm) 569
分子量 1185.56 溶剂 DMSO
产品详细介绍

简要概述

产品基本信息

货号:920

产品名称:花菁素DBCO-Cy3

CAS:1782950-79-1

规格:1mg

储存条件:-15℃避光防潮

保质期:12个月

 

产品物理化学光谱特性

分子量:1185.56

溶剂:DMSO

激发波长(nm):555

发射波长(nm):565

 

产品介绍

花菁素DBCO-Cy3是美国AAT Bioquest生产的花菁类荧光染料。该氮杂二苯并环辛炔 – 花青染料衍生物是用于检测含叠氮基的分子或化合物的通用标记试剂。环辛炔可用于菌株促进的无铜叠氮化物 – 炔烃环加成反应。该二苯并环辛炔将与叠氮官能化化合物或生物分子反应,而不需要Cu(I)催化剂以产生稳定的三唑键。AAT Bioquest提供多种染料叠氮化合物(如香豆素叠氮化物,荧光素叠氮化物,罗丹明叠氮化物和花青叠氮化物)用于点击反应。金畔生物是AAT Bioquest的中国代理商,为您提供最优质的花菁素DBCO-Cy3。 

点击查看光谱

 

参考文献

Site-specific chemical conjugation of human Fas ligand extracellular domain using trans-cyclooctene–methyltetrazine reactions
Authors: Michiro Muraki, Kiyonori Hirota
Journal: BMC biotechnology (2017): 56

说明书
花菁素DBCO-Cy3 CAS 1782950-79-1.pdf

DBCO-Cy5 货号923-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。

DBCO-Cy5

DBCO-Cy5

DBCO-Cy5    货号923 货号 923 存储条件 在零下15度以下保存, 避免光照
规格 1 mg 价格 1272
Ex (nm) 651 Em (nm) 670
分子量 1211.60 溶剂 DMSO
产品详细介绍

简要概述

产品基本信息

货号:923

产品名称:DBCO-Cy5

规格:1mg

储存条件:-15℃避光防潮

保质期:12个月

 

产品物理化学光谱特性

分子量:1211.60

溶剂:DMSO

激发波长(nm):650

发射波长(nm):669

 

产品介绍

DBCO-Cy5是美国AAT Bioquest生产的荧光染料。氨基易与活化的羧酸酯(如NHS酯)反应。金畔生物是AAT Bioquest的中国代理商,为您提供最优质的DBCO-Cy5。 

点击查看光谱

 

参考文献

Site-specific chemical conjugation of human Fas ligand extracellular domain using trans-cyclooctene–methyltetrazine reactions
Authors: Michiro Muraki, Kiyonori Hirota
Journal: BMC biotechnology (2017): 56

说明书
DBCO-Cy5.pdf

DBCO 琥珀酰亚胺酯 CAS 1353016-71-3 货号4529-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。

DBCO 琥珀酰亚胺酯 CAS 1353016-71-3

DBCO 琥珀酰亚胺酯 CAS 1353016-71-3

DBCO 琥珀酰亚胺酯 CAS 1353016-71-3    货号4529 货号 4529 存储条件 在零下15度以下保存, 避免光照
规格 10 mg 价格 1272
Ex (nm) Em (nm)
分子量 402.41 溶剂 DMSO
产品详细介绍

简要概述

产品基本信息

货号:4529

产品名称:DBCO 琥珀酰亚胺酯

CAS:1353016-71-3

规格:10mg

储存条件:-15℃避光防潮

保质期:24个月

 

产品物理化学光谱特性

分子量:402.41

外观:固体

溶剂:DMSO

 

产品介绍

无铜点击化学反应,即菌株促进的叠氮炔环加成(SPAAC)是一种生物正交反应,利用一对试剂,即环辛基和叠氮化合物,它们彼此有效地反应,同时对羟基、氨基和硫醇等天然官能团保持惰性。SPAAC能够通过形成稳定的三唑,在水和其他复杂的化学环境中标记各种各样的生物分子,而无需任何辅助试剂。在众多已知的环辛基化合物中,二苯并环辛基化合物(DBCO)是一类具有相当快的动力学和良好的水缓冲稳定性的试剂。在生理温度和pH范围内,DBCO基团不会与许多生物分子中天然存在的巯基、氨基或羟基发生反应。DBCO-琥珀酰亚胺酯是一种常用的通过氨基修饰将DBCO基团引入生物分子的试剂。金畔生物是AAT Bioquest的中国代理商,为您提供最优质的DBCO 琥珀酰亚胺酯。 

说明书
DBCO 琥珀酰亚胺酯 CAS 1353016-71-3.pdf

淬灭剂DABCYL-DBCO 货号2010-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。

淬灭剂DABCYL-DBCO

淬灭剂DABCYL-DBCO

淬灭剂DABCYL-DBCO    货号2010 货号 2010 存储条件 在零下15度以下保存, 避免光照
规格 5 mg 价格 3732
Ex (nm) Em (nm)
分子量 527.63 溶剂 DMSO
产品详细介绍

简要概述

产品基本信息

货号:2010

产品名称:淬灭剂DABCYL-DBCO

规格:5mg

储存条件:-15℃避光防潮

保质期:12个月

 

产品物理化学光谱特性

分子量:527.63

溶剂:DMSO

 

产品介绍

DABCYL是用于制备各种基于FRET的核酸探针和蛋白酶底物的最常见的淬灭剂之一。它是一种吸收来自荧光团的激发能并将其作为热量消散的物质。当它和荧光团紧密靠近时,例如在分子或蛋白质中,荧光团的发射受到抑制。此效应可用于研究分子的几何形状和运动。DABCYL-DBCO是通过基于DBCO的点击化学开发FRET探针,该化学是最著名的应变促进叠氮化物-炔烃环加成反应(SPAAC)之一。金畔生物是AAT Bioquest的中国代理商,为您提供最优质的淬灭剂DABCYL-DBCO。

 

参考文献

A Cell Membrane-Targeting Self-Delivery Chimeric Peptide for Enhanced Photodynamic Therapy and In Situ Therapeutic Feedback.
Authors: Ma, Wen and Sha, Sui-Nan and Chen, Pei-Ling and Yu, Meng and Chen, Jian-Jun and Huang, Chao-Bo and Yu, Bin and Liu, Yun and Liu, Li-Han and Yu, Zhi-Qiang
Journal: Advanced healthcare materials (2020): e1901100

Array-based in situ fluorescence assay for profiling multiplex matrix metalloproteinases activities in tissue section.
Authors: Lei, Zhen and Jian, Minghong and Wei, Jia and Wang, Yaoqi and Meng, Xianying and Wang, Zhenxin
Journal: Analytica chimica acta (2019): 112-118

FRET-Based Assays to Determine Calpain Activity.
Authors: McCartney, Christian-Scott E and Davies, Peter L
Journal: Methods in molecular biology (Clifton, N.J.) (2019): 39-55

Polymeric fluorescent heparin as one-step FRET substrate of human heparanase.
Authors: Sistla, Jyothi C and Morla, Shravan and Alabbas, Al-Humaidi B and Kalathur, Ravi C and Sharon, Chetna and Patel, Bhaumik B and Desai, Umesh R
Journal: Carbohydrate polymers (2019): 385-391

The conversion of azo-quenchers to fluorophores.
Authors: Hofstetter, O and Hofstetter, H and Miron, T and Wilchek, M
Journal: Analytical biochemistry (2019): 113400

A FRET-based dual-color evanescent wave optical fiber aptasensor for simultaneous fluorometric determination of aflatoxin M1 and ochratoxin A.
Authors: Song, Dan and Yang, Rong and Fang, Shunyan and Liu, Yanping and Long, Feng
Journal: Mikrochimica acta (2018): 508

Development of a peptide substrate for detection of sunn pest damage in wheat flour.
Authors: Hançerlioğulları, Begüm Zeynep and Köksel, Hamit and Dudak, Fahriye Ceyda
Journal: Journal of the science of food and agriculture (2018): 5677-5682

A dual-colored ratiometric-fluorescent oligonucleotide probe for the detection of human telomerase RNA in cell extracts.
Authors: Ning, Dianhua and He, Changtian and Liu, Zhengjie and Liu, Cui and Wu, Qilong and Zhao, TingTing and Liu, Renyong
Journal: The Analyst (2017): 1697-1702

Chemoenzymatic synthesis of fluorogenic phospholipids and evaluation in assays of phospholipases A, C and D.
Authors: Piel, Mathilde S and Peters, Günther H J and Brask, Jesper
Journal: Chemistry and physics of lipids (2017): 49-54

Detection of human neutrophil elastase (HNE) on wound dressings as marker of inflammation.
Authors: Ferreira, Ana V and Perelshtein, Ilana and Perkas, Nina and Gedanken, Aharon and Cunha, Joana and Cavaco-Paulo, Artur
Journal: Applied microbiology and biotechnology (2017): 1443-1454

说明书
淬灭剂DABCYL-DBCO.pdf