钙离子荧光探针Fluo-5F, AM
 |
货号 |
20560 |
存储条件 |
在零下15度以下保存, 避免光照 |
| 规格 |
10×50 ug |
价格 |
2604 |
| Ex (nm) |
494 |
Em (nm) |
516 |
| 分子量 |
1100.91 |
溶剂 |
DMSO |
| 产品详细介绍 |
简要概述
产品基本信息
货号：20560
产品名称：钙离子荧光探针Fluo-5F, AM
规格：10×50 ug
储存条件：-15℃避光防潮
保质期：24个月
产品物理化学光谱特性
分子量：1100.91
溶剂：DMSO
激发波长(nm)：494
发射波长(nm)：516
适用仪器
| 流式细胞仪 |
|
| 激发： |
488nm激发 |
| 发射： |
530/30nm滤波片 |
| 通道： |
FITC通道 |
| 荧光显微镜 |
|
| 激发： |
FITC |
| 发射： |
FITC |
| 推荐孔板： |
黑色透明 |
| 荧光酶标仪 |
|
| 激发： |
490nm |
| 发射： |
525nm |
| cutoff: |
515nm |
| 推荐孔板： |
黑色透明 |
| 读取模式： |
底读模式 |
产品介绍
钙离子荧光探针Fluo-5F, AM是美国AAT Bioquest生产的钙离子荧光探针，Fluo-5F是Fluo-4的类似物，具有较低的钙结合亲和力（Kd =〜2.3 uM），使其适合检测1 µM至1 mM的细胞内钙水平，该水平会饱和Fluo-4的响应。通过将溶解的指示剂直接添加到含有培养细胞的培养皿中，可以在细胞中加载Fluo-5F AM酯。它与氩离子激光源在488 nm处激发兼容，使Fluo-5F可用于共聚焦显微镜，流式细胞仪和微孔板筛选应用。它的激发和发射波长分别为494和516 nm。钙结合后，其荧光强度增加> 100倍。金畔生物是AAT Bioquest的中国代理商，为您提供最优质的钙离子荧光探针Fluo-5F, AM。
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钙离子篇：时间轴式讲解应用于钙离子检测的探针
参考文献
Calreticulin regulates TGF-β1-induced epithelial mesenchymal transition through modulating Smad signaling and calcium signaling
Authors: Yanjiao Wu, Xiaoli Xu, Lunkun Ma, Qian Yi, Weichao Sun, Liling Tang
Journal: The International Journal of Biochemistry & Cell Biology (2017)
Dexmedetomidine reduces hypoxia/reoxygenation injury by regulating mitochondrial fission in rat hippocampal neurons
Authors: Jia Liu, Qing Du, He Zhu, Yu Li, Maodong Liu, Shoushui Yu, Shilei Wang
Journal: Int J Clin Exp Med (2017): 6861–6868
Monosialoganglioside 1 may alleviate neurotoxicity induced by propofol combined with remifentanil in neural stem cells
Authors: Jiang Lu, Xue-qin Yao, Xin Luo, Yu Wang, Sookja Kim Chung, He-xin Tang, Chi Wai Cheung, Xian-yu Wang, Chen Meng, Qing Li
Journal: Neural Regeneration Research (2017): 945
Obtaining spontaneously beating cardiomyocyte-like cells from adipose-derived stromal vascular fractions cultured on enzyme-crosslinked gelatin hydrogels
Authors: Gang Yang, Zhenghua Xiao, Xiaomei Ren, Haiyan Long, Kunlong Ma, Hong Qian, Yingqiang Guo
Journal: Scientific Reports (2017): 41781
Di (2-ethylhexyl) phthalate-induced apoptosis in rat INS-1 cells is dependent on activation of endoplasmic reticulum stress and suppression of antioxidant protection
Authors: Xia Sun, Yi Lin, Qiansheng Huang, Junpeng Shi, Ling Qiu, Mei Kang, Yajie Chen, Chao Fang, Ting Ye, Sijun Dong
Journal: Journal of cellular and molecular medicine (2015): 581–594
The effect of mitochondrial calcium uniporter on mitochondrial fission in hippocampus cells ischemia/reperfusion injury
Authors: Lantao Zhao, Shuhong Li, Shilei Wang, Ning Yu, Jia Liu
Journal: Biochemical and biophysical research communications (2015): 537–542
Fungus induces the release of IL-8 in human corneal epithelial cells, via Dectin-1-mediated protein kinase C pathways.
Authors: Xu-Dong Peng, Gui-Qiu Zhao, Jing Lin, Nan Jiang, Qiang Xu, Cheng-Cheng Zhu, Jain-Qiu Qu, Lin Cong, Hui Li
Journal: International journal of ophthalmology (2014): 441–447
Propofol and remifentanil at moderate and high concentrations affect proliferation and differentiation of neural stem/progenitor cells
Authors: Qing Li, Jiang Lu, Xianyu Wang
Journal: Neural regeneration research (2014): 2002
Role of mitochondrial calcium uniporter in regulating mitochondrial fission in the cerebral cortexes of living rats
Authors: Nan Liang, Peng Wang, Shilei Wang, Shuhong Li, Yu Li, Jinying Wang, Min Wang
Journal: Journal of Neural Transmission (2014): 593–600
Increased expression of cell adhesion molecule 1 by mast cells as a cause of enhanced nerve–mast cell interaction in a hapten-induced mouse model of atopic dermatitis
Authors: M Hagiyama, T Inoue, T Furuno, T Iino, S Itami, M Nakanishi, H Asada, Y Hosokawa, A Ito
Journal: British Journal of Dermatology (2013): 771–778
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说明书
钙离子荧光探针Fluo-5F, AM.pdf
