HIS Lite Cy3 Bis NTA-Ni 复合物
 |
货号 |
12610 |
存储条件 |
在零下15度以下保存, 避免光照 |
| 规格 |
1 mg |
价格 |
5244 |
| Ex (nm) |
555 |
Em (nm) |
569 |
| 分子量 |
1384.66 |
溶剂 |
Water |
| 产品详细介绍 |
简要概述
产品基本信息
货号：12610
产品名称：HIS Lite Cy3 Bis NTA-Ni 复合物
规格：1mg
储存条件：-15℃避光防潮
保质期：24个月
产品物理化学光谱特性
分子量：1356.60
溶剂：DMSO
激发波长(nm)：555
发射波长(nm)：569
产品介绍
聚组氨酸是掺入重组蛋白中最流行的亲和标签之一。它可以插入N端或C端，并在多种宿主中表达。由于其体积小，聚组氨酸标签可作为蛋白质纯化和检测的理想工具。 HIS Lite™Cy3 Bis NTA-Ni和Cy5 Bis NTA-Ni复合物可对His标记的融合蛋白进行特异性和高灵敏度的检测。 Ni-NTA配合物最早由Kapanidis等报道。对具有最小交叉反应性的多组氨酸标签具有特异性。 Cy3和Cy5染料在通常可用的波长处显示强荧光信号，几乎没有猝灭。 Cy3 Bis NTA-Ni和Cy5 Bis NTA-Ni复合物可直接应用于SDS-PAGE凝胶或Western blot膜进行荧光成像。用Cy3 Bis NTA-Ni和Cy5 Bis NTA-Ni复合物进行检测所需的孵育时间少于蛋白质-抗体结合所需的孵育时间。由于Ni-NTA络合物直接与荧光团偶联，因此不需要二次反应。
参考文献
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A facile method for reversibly linking a recombinant protein to DNA
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A pair of ligation-independent Escherichia coli expression vectors for rapid addition of a polyhistidine affinity tag to the N- or C-termini of recombinant proteins
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Journal: J Biomol Tech (2009): 241
RNA aptamer binding to polyhistidine-tag
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Journal: Biochem Biophys Res Commun (2009): 227
说明书
HIS Lite Cy3 Bis NTA-Ni 复合物.pdf
