PRO-TAO-3 [相当于TO-PRO-3](停产) CAS 157199-63-8 货号17565-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。

PRO-TAO-3 [相当于TO-PRO-3](停产) CAS 157199-63-8

PRO-TAO-3 [相当于TO-PRO-3](停产) CAS 157199-63-8

PRO-TAO-3 [相当于TO-PRO-3](停产) CAS 157199-63-8    货号17565 货号 17565 存储条件 在零下15度以下保存, 避免光照
规格 1 mL 价格 0
Ex (nm) 642 Em (nm) 657
分子量 671.42 溶剂 DMSO
产品详细介绍

简要概述

产品基本信息

货号:17565

产品名称:PRO-TAO-3 [相当于TO-PRO-3]

CAS:157199-63-8

规格:1 ml

储存条件:-15℃避光防潮

保质期:12个月

 

产品物理化学光谱特性

外观:液体

分子量:671.42

溶剂:水

Ex(nm):642

Em(nm):657

 

产品介绍

PRO-TAO-3与TO-PRO®-3染色分子相同。TO-PRO®-3和其他单体花菁染料由于背景低和荧光明亮而具有广泛的适用性。它是一种碳花青素单体核酸染色剂,可以用633 nm或647 nm的红色激发光很好地激发,并具有类似于Cy®5染料的远红色荧光。它可用作核复染色和死细胞指示剂。它对检测dsDNA(双链核酸)具有选择性。不可渗透细胞的PRO-TTO-3被广泛用于流式细胞术和荧光显微镜检查,因为长波长荧光在多色应用中已与绿色和红色荧光团充分分离。TO-PRO®是ThermoFisher Scientific的商标。

点击查看光谱

 

参考文献

Anti-inflammatory functions of the glucocorticoid receptor require DNA binding.
Authors: Escoter-Torres, Laura and Greulich, Franziska and Quagliarini, Fabiana and Wierer, Michael and Uhlenhaut, Nina Henriette
Journal: Nucleic acids research (2020)

DNA methylation dynamics underlie metamorphic gene regulation programs in Xenopus tadpole brain.
Authors: Kyono, Yasuhiro and Raj, Samhitha and Sifuentes, Christopher J and Buisine, Nicolas and Sachs, Laurent and Denver, Robert J
Journal: Developmental biology (2020): 180-196

DNA-PKcs has KU-dependent function in rRNA processing and haematopoiesis.
Authors: Shao, Zhengping and Flynn, Ryan A and Crowe, Jennifer L and Zhu, Yimeng and Liang, Jialiang and Jiang, Wenxia and Aryan, Fardin and Aoude, Patrick and Bertozzi, Carolyn R and Estes, Verna M and Lee, Brian J and Bhagat, Govind and Zha, Shan and Calo, Eliezer
Journal: Nature (2020): 291-296

Mithramycin selectively attenuates DNA-damage-induced neuronal cell death.
Authors: Makarevich, Oleg and Sabirzhanov, Boris and Aubrecht, Taryn G and Glaser, Ethan P and Polster, Brian M and Henry, Rebecca J and Faden, Alan I and Stoica, Bogdan A
Journal: Cell death & disease (2020): 587

Persistent DNA damage triggers activation of the integrated stress response to promote cell survival under nutrient restriction.
Authors: Clementi, Elena and Inglin, Larissa and Beebe, Erin and Gsell, Corina and Garajova, Zuzana and Markkanen, Enni
Journal: BMC biology (2020): 36

Acute aluminum chloride toxicity revisited: Study on DNA damage and histopathological, biochemical and neurochemical alterations in rat brain.
Authors: Liaquat, Laraib and Sadir, Sadia and Batool, Zehra and Tabassum, Saiqa and Shahzad, Sidrah and Afzal, Asia and Haider, Saida
Journal: Life sciences (2019): 202-211

Selectivity of Terpyridine Platinum Anticancer Drugs for G-quadruplex DNA.
Authors: Morel, Elodie and Beauvineau, Claire and Naud-Martin, Delphine and Landras-Guetta, Corinne and Verga, Daniela and Ghosh, Deepanjan and Achelle, Sylvain and Mahuteau-Betzer, Florence and Bombard, Sophie and Teulade-Fichou, Marie-Paule
Journal: Molecules (Basel, Switzerland) (2019)

DNA damage regulated autophagy modulator 1 recovers the function of apoptosis-stimulating of p53 protein 2 on inducing apoptotic cell death in Huh7.5 cells.
Authors: Liu, Dongjie and Li, Rui and Guo, Xianghua and Pang, Lijun and Zang, Yunjin and Liu, Kai and Chen, Dexi
Journal: Oncology letters (2018): 9333-9338

Regular exercise participation improves genomic stability in diabetic patients: an exploratory study to analyse telomere length and DNA damage.
Authors: Dimauro, Ivan and Sgura, Antonella and Pittaluga, Monica and Magi, Fiorenza and Fantini, Cristina and Mancinelli, Rosa and Sgadari, Antonio and Fulle, Stefania and Caporossi, Daniela
Journal: Scientific reports (2017): 4137

Redox-linked effects of green tea on DNA damage and repair, and influence of microsatellite polymorphism in HMOX-1: results of a human intervention trial.
Authors: Choi, Siu-Wai and Yeung, Vincent T F and Collins, Andrew R and Benzie, Iris F F
Journal: Mutagenesis (2015): 129-37

说明书
PRO-TAO-3 [相当于TO-PRO-3](停产) CAS 157199-63-8.pdf

死细胞核染料 TWO-PRO 1 相当于TO-PRO 1 CAS 157199-59-2 货号17571-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。

死细胞核染料 TWO-PRO 1 相当于TO-PRO 1 CAS 157199-59-2

死细胞核染料 TWO-PRO 1 相当于TO-PRO 1 CAS 157199-59-2

死细胞核染料 TWO-PRO 1  相当于TO-PRO 1  CAS 157199-59-2    货号17571 货号 17571 存储条件 在零下15度以下保存, 避免光照
规格 0.2 ml 价格 2604
Ex (nm) 515 Em (nm) 531
分子量 645.38 溶剂 DMSO
产品详细介绍

简要概述

产品基本信息

货号:17571

产品名称:死细胞核染料 TWO-PRO 1  相当于TO-PRO 1 

CAS:157199-59-2

规格:0.2ml

储存条件:-15℃避光干燥

保质期:12个月

 

产品物理化学光谱特性

分子量:645.38

激发波长(nm):515

发射波长(nm):531

 

适用仪器


荧光显微镜  
激发: FITC滤波片
发射: FITC滤波片
推荐孔板: 黑色透明

 

产品介绍

TWO-PRO -1在化学上等同于TO-PRO®-1(TO-PRO®是Invitrogen的商标)。TWO-PRO -1是一种碳花青二聚体,具有类似于FITC的绿色荧光。它不具有细胞渗透性,可以很容易地与Cy5和罗丹明区别开来,作为核复染和死细胞指示剂。在不存在核酸的情况下它是非荧光的,并且在与DNA结合后会产生非常明亮的荧光信号。TWO-PRO -1可在培养细胞和石蜡切片中进行强而有选择性的核染色。同时使用细胞可渗透的Nuclear Red LCS1染料和不渗透细胞的TWO-PRO -1进行标记,可用于评估细胞活力。TWO-PRO -1和Nuclear Red 的消光系数都比结合DNA的碘化丙啶大。

点击查看光谱

 

参考文献

Daptomycin exerts rapid bactericidal activity against Bacillus anthracis without disrupting membrane integrity
Authors: Xing YH, Wang W, Dai SQ, Liu TY, Tan JJ, Qu GL, Li YX, Ling Y, Liu G, Fu XQ, Chen HP.
Journal: Acta Pharmacol Sin (2014): 211

A silicon cell cycle in a bacterial model of calcium phosphate mineralogenesis
Authors: Linton KM, Tapping CR, Adams DG, Carter RD, Shore RC, Aaron JE.
Journal: Micron (2013): 419

Quantification of Candida albicans by flow cytometry using TO-PRO((R))-3 iodide as a single-stain viability dye
Authors: Kerstens M, Boulet G, Pintelon I, Hellings M, Voeten L, Delputte P, Maes L, Cos P.
Journal: J Microbiol Methods (2013): 189

A comparison of TO-PRO-1 iodide and 5-CFDA-AM staining methods for assessing viability of planktonic algae with epifluorescence microscopy
Authors: Gorokhova E, Mattsson L, Sundstrom AM.
Journal: J Microbiol Methods (2012): 216

Determination of the drug-DNA binding modes using fluorescence-based assays
Authors: Williams AK, Dasilva SC, Bhatta A, Rawal B, Liu M, Korobkova EA.
Journal: Anal Biochem (2012): 66

Pulsed electromagnetic field affects intrinsic and endoplasmatic reticulum apoptosis induction pathways in MonoMac6 cell line culture
Authors: Kaszuba-Zwoinska J, Chorobik P, Juszczak K, Zaraska W, Thor PJ.
Journal: J Physiol Pharmacol (2012): 537

Transient changes in neuronal cell membrane permeability after blast exposure
Authors: Arun P, Abu-Taleb R, Valiyaveettil M, Wang Y, Long JB, Nambiar MP.
Journal: Neuroreport (2012): 342

SOCS-3 antagonizes pro-apoptotic effects of TRAIL and resveratrol in prostate cancer cells
Authors: Horndasch M, Culig Z.
Journal: Prostate (2011): 1357

The fluorescent dyes TO-PRO-3 and TOTO-3 iodide allow detection of microbial cells in soil samples without interference from background fluorescence
Authors: Henneberger R, Birch D, Bergquist P, Walter M, Anitori RP.
Journal: Biotechniques (2011): 190

Impact of mitochondriotropic quercetin derivatives on mitochondria
Authors: Biasutto L, Sassi N, Mattarei A, Marotta E, Cattelan P, Toninello A, Garbisa S, Zoratti M, Paradisi C.
Journal: Biochim Biophys Acta (2010): 189

说明书
死细胞核染料 TWO-PRO 1 相当于TO-PRO 1 CAS 157199-59-2.pdf

蛋白酶荧光底物Gly-Pro-AMC CAS 115035-46-6 货号13450-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。

蛋白酶荧光底物Gly-Pro-AMC CAS 115035-46-6

蛋白酶荧光底物Gly-Pro-AMC CAS 115035-46-6

蛋白酶荧光底物Gly-Pro-AMC  CAS 115035-46-6    货号13450 货号 13450 存储条件 在零下15度以下保存, 避免光照
规格 5 mg 价格 1272
Ex (nm) 341 Em (nm) 441
分子量 443.37 溶剂 DMSO
产品详细介绍

简要概述

蛋白酶荧光底物Gly-Pro-AMC是美国AAT Bioquest生产的荧光染料,Gly-Pro-AMC是用于检测二肽酰多肽酶IV (DPPIV)的高灵敏度荧光底物,一种丝氨酸蛋白酶,能够从倒数第二位上是L-脯氨酸或者L-丙氨酸的多肽上剪切其N末端的二肽酰。DPPIV是表达于多种细胞(包括上皮细胞,内皮细胞和淋巴细胞)表面的多功能蛋白质。它与T细胞活化抗原CD26和腺嘌呤核苷脱氨基酶(腺苷脱氨酶)结合蛋白具有同一性,它以可溶性状态释放于血浆中。CD26/DPPIV的底物包括多种含有脯氨酸的多肽,例如生长因子、趋化因子、神经肽和血管活性肽。DPPIV参与免疫调控,信号转导和细胞凋亡,并且在肿瘤的发展中发挥重要作用。重要的是,DPPIV是II型糖尿病的治疗靶点,由于其作为血清蛋白酶,能够剪切多肽胰高血糖素家族中肠降血糖素荷尔蒙,以此调节体内葡糖糖的稳定。研究显示一种DPPIV抑制剂可以增强葡糖糖衰减的耐受力。用带有Gly-Pro-AMC的DPPIV检测是一种简单、快速,灵活的检测法,是理想的高通量筛选方法。与DPPIV检测法相比,这种检测法增强了检测灵敏度,使研究者可以用更少的酶,同时获得恰当的Z值。这种单一性的酶偶联形式使用非常方便,只需要在检测样品中添加一种单一的试剂;在20inm内可以获得最大感光值,并且其单一产物非常稳定。金畔生物是AAT Bioquest的中国代理商,为您提供最优质的蛋白酶荧光底物Gly-Pro-AMC。 

点击查看光谱

 

适用仪器


荧光酶标仪  
激发: 380nm
发射: 500nm
cutoff: 435nm
推荐孔板: 黑色孔板

产品说明书

样品实验方案 

溶液制备 

1.储备溶液

所有未使用的储备溶液应分为一次性使用的等分试样,并在制备后储存在-20°C下。 避免重复冻融循环。
1.1 Gly-Pro-AMC:
在DMSO中制成10至25 mM的Gly-Pro-AMC储备溶液,避光。 注意:储备溶液应及时使用; 应将所有未使用的溶液等分,并在-20℃冷冻。注意:避免重复冻融循环,并避免光照。

 

2.工作溶液

Gly-Pro-AMC:
取出一个小瓶,并用您选择的测定缓冲液(例如50 mM Tris-HCl,pH 7.5,1.0 mM DTT)稀释储备溶液,制成50至100 µM测定溶液。

 

样品操作及分析

1.将等体积的DPPIV标准品或样品与测定溶液混合,并在室温下孵育至少1小时。

2.在Ex / Em = 380/500 nm处监测荧光增加。

 

参考文献

Effects of activated carbon N-acetylcysteine sustained-release microcapsule on dipeptidyl peptidase IV expression in young rats with non-alcoholic fatty liver disease
Authors: Hongping Zhou, Tingting Shi, Jun Yan, Xiaojin Chen, Li Liao, Shiyong Zhao, Hongying Fang, Rangxiao Zhuang
Journal: Experimental and Therapeutic Medicine (2017): 4737–4744

说明书
蛋白酶荧光底物Gly-Pro-AMC CAS 115035-46-6.pdf

蛋白酶荧光底物Ac-Pro-Ala-Leu-AMC 货号13479-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。

蛋白酶荧光底物Ac-Pro-Ala-Leu-AMC

蛋白酶荧光底物Ac-Pro-Ala-Leu-AMC

蛋白酶荧光底物Ac-Pro-Ala-Leu-AMC    货号13479 货号 13479 存储条件 在零下15度以下保存, 避免光照
规格 5 mg 价格 1272
Ex (nm) 341 Em (nm) 441
分子量 498.57 溶剂 DMSO
产品详细介绍

简要概述

产品基本信息

货号:13479

产品名称:蛋白酶荧光底物Ac-Pro-Ala-Leu-AMC

规格:5mg

储存条件:-15℃避光防潮

保质期:24个月

 

产品物理化学光谱特性

分子量:498.57

溶剂:DMSO

激发波长(nm):351

发射波长(nm):430

 

产品介绍

蛋白酶荧光底物Ac-Pro-Ala-Leu-AMC是美国AAT Bioquest生产的荧光底物,弱荧光AMC基板产生亮蓝色荧光AMC产品,其具有Ex / Em = 351 / 430nm,并且可以使用DAPI滤光器组容易地检测。该AMC底物用于监测蛋白酶体的蛋白酶活性。最常见的蛋白酶体形式称为26S蛋白酶体,其含有一个20S核心颗粒结构和两个19S调节帽。所有20S颗粒由四个堆叠的七聚环结构组成,它们本身由两种不同类型的亚基组成; α亚基本质上是结构性的,而β亚基主要是催化性的。堆叠中的外部两个环由每个七个α亚基组成,其用作调节颗粒的对接域,并且α亚基N末端形成阻止底物未受调节进入内腔的门。内部两个环各自由7个β亚基组成,并含有进行蛋白水解反应的蛋白酶活性位点。金畔生物是AAT Bioquest的中国代理商,为您提供最优质的蛋白酶荧光底物Ac-Pro-Ala-Leu-AMC。 

点击查看光谱

 

参考文献

Diabetogenic agent alloxan is a proteasome inhibitor
Authors: Wenjuan Zhou, Lingling Wei, Ting Xiao, Chunyou Lai, Min Peng, Lingli Xu, Xiangwei Luo, Shaoping Deng, Fengxue Zhang
Journal: Biochemical and Biophysical Research Communications (2017): 400–406

Delineation of molecular pathways involved in cardiomyopathies caused by troponin T mutations
Authors: Jennifer E Gilda, Xianyin Lai, Frank A Witzmann, Aldrin V Gomes
Journal: Molecular & Cellular Proteomics (2016): 1962–1981

Diclofenac induces proteasome and mitochondrial dysfunction in murine cardiomyocytes and hearts
Authors: Rajeshwary Ghosh, Sumanta K Goswami, Luis Felipe BB Feitoza, Bruce Hammock, Aldrin V Gomes
Journal: International Journal of Cardiology (2016): 923–935

Advanced-glycation-end-product-induced formation of immunoproteasomes: involvement of RAGE and Jak2/STAT1
Authors: Stefanie Grimm, Christiane Ott, Melanie Hörlacher, Daniela Weber, Annika Höhn, Tilman Grune
Journal: Biochemical Journal (2012): 127–139

说明书
蛋白酶荧光底物Ac-Pro-Ala-Leu-AMC.pdf

死细胞核染料 TWO-PRO 3 相当于TO-PRO 3 CAS 157199-63-8 货号17572-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。

死细胞核染料 TWO-PRO 3 相当于TO-PRO 3 CAS 157199-63-8

死细胞核染料 TWO-PRO 3 相当于TO-PRO 3 CAS 157199-63-8

死细胞核染料 TWO-PRO 3  相当于TO-PRO 3  CAS 157199-63-8    货号17572 货号 17572 存储条件 在零下15度以下保存, 避免光照
规格 0.2 ml 价格 2604
Ex (nm) 642 Em (nm) 657
分子量 671.42 溶剂 DMSO
产品详细介绍

简要概述

产品基本信息

货号:17572

产品名称:死细胞核染料 TWO-PRO 3  相当于TO-PRO 3 

CAS:157199-63-8

规格:0.2ml

储存条件:-15℃避光干燥

保质期:12个月

 

产品物理化学光谱特性

分子量:671.42

溶剂:DMSO

激发波长(nm):642

发射波长(nm):661

 

适用仪器


荧光显微镜  
激发: Cy5滤波片
发射: Cy5滤波片
推荐孔板: 黑色透明

 

产品介绍

TWO-PRO -1在化学上等同于TO-PRO®-1(TO-PRO®是Invitrogen的商标)。TWO-PRO -1是一种碳花青二聚体,具有类似于FITC的绿色荧光。它不具有细胞渗透性,可以很容易地与Cy5和罗丹明区别开来,作为核复染和死细胞指示剂。在不存在核酸的情况下它是非荧光的,并且在与DNA结合后会产生非常明亮的荧光信号。TWO-PRO -1可在培养细胞和石蜡切片中进行强而有选择性的核染色。同时使用细胞可渗透的Nuclear Red LCS1染料和不渗透细胞的TWO-PRO -1进行标记,可用于评估细胞活力。TWO-PRO -1和Nuclear Red 的消光系数都比结合DNA的碘化丙啶大。

点击查看光谱

 

参考文献

Daptomycin exerts rapid bactericidal activity against Bacillus anthracis without disrupting membrane integrity
Authors: Xing YH, Wang W, Dai SQ, Liu TY, Tan JJ, Qu GL, Li YX, Ling Y, Liu G, Fu XQ, Chen HP.
Journal: Acta Pharmacol Sin (2014): 211

A silicon cell cycle in a bacterial model of calcium phosphate mineralogenesis
Authors: Linton KM, Tapping CR, Adams DG, Carter RD, Shore RC, Aaron JE.
Journal: Micron (2013): 419

Quantification of Candida albicans by flow cytometry using TO-PRO((R))-3 iodide as a single-stain viability dye
Authors: Kerstens M, Boulet G, Pintelon I, Hellings M, Voeten L, Delputte P, Maes L, Cos P.
Journal: J Microbiol Methods (2013): 189

A comparison of TO-PRO-1 iodide and 5-CFDA-AM staining methods for assessing viability of planktonic algae with epifluorescence microscopy
Authors: Gorokhova E, Mattsson L, Sundstrom AM.
Journal: J Microbiol Methods (2012): 216

Determination of the drug-DNA binding modes using fluorescence-based assays
Authors: Williams AK, Dasilva SC, Bhatta A, Rawal B, Liu M, Korobkova EA.
Journal: Anal Biochem (2012): 66

Pulsed electromagnetic field affects intrinsic and endoplasmatic reticulum apoptosis induction pathways in MonoMac6 cell line culture
Authors: Kaszuba-Zwoinska J, Chorobik P, Juszczak K, Zaraska W, Thor PJ.
Journal: J Physiol Pharmacol (2012): 537

Transient changes in neuronal cell membrane permeability after blast exposure
Authors: Arun P, Abu-Taleb R, Valiyaveettil M, Wang Y, Long JB, Nambiar MP.
Journal: Neuroreport (2012): 342

SOCS-3 antagonizes pro-apoptotic effects of TRAIL and resveratrol in prostate cancer cells
Authors: Horndasch M, Culig Z.
Journal: Prostate (2011): 1357

The fluorescent dyes TO-PRO-3 and TOTO-3 iodide allow detection of microbial cells in soil samples without interference from background fluorescence
Authors: Henneberger R, Birch D, Bergquist P, Walter M, Anitori RP.
Journal: Biotechniques (2011): 190

Impact of mitochondriotropic quercetin derivatives on mitochondria
Authors: Biasutto L, Sassi N, Mattarei A, Marotta E, Cattelan P, Toninello A, Garbisa S, Zoratti M, Paradisi C.
Journal: Biochim Biophys Acta (2010): 189

说明书
死细胞核染料 TWO-PRO 3 相当于TO-PRO 3 CAS 157199-63-8.pdf