ReadiUse 预活化别藻蓝蛋白APC

	货号	2561	存储条件	Multiple
	规格	1 mg	价格	3924
	Ex (nm)	651	Em (nm)	660
	分子量		溶剂
	产品详细介绍

简要概述

产品基本信息

货号：2561

产品名称：ReadiUse 预活化别藻蓝蛋白APC

规格：1mg

储存条件：2-8℃避光防潮

保质期：12个月

产品物理化学光谱特性

分子量：N/A

溶剂：水

激发波长(nm)：651

发射波长(nm)：713

产品介绍

ReadiUse 预活化别藻蓝蛋白APC是美国AAT Bioquest生产的蛋白标记染料，别藻蓝蛋白（APC）是从螺旋藻（一种蓝绿藻）中分离的藻胆蛋白。 与其他藻胆蛋白一样，APC是荧光的，具有极高的吸收率和高量子效率。 它是一种蛋白质，通过常规蛋白质交联技术可以很容易地与抗体和其他蛋白质连接，而不会改变其光谱特征。 AAT Bioquest提供这种预活化的APC，以促进APC与抗体和其他蛋白质如链霉抗生物素蛋白和其他二级试剂的结合。 我们的预活化APC已准备好结合，产生比常规繁琐的基于SMCC的缀合化学高得多的产率。 此外，我们的预活化APC通过其蛋白质丰富的氨基与蛋白质缀合，而SMCC化学靶向必须通过抗体还原而再生的硫醇基团。金畔生物是AAT Bioquest的中国代理商，为您提供最优质的ReadiUse 预活化别藻蓝蛋白APC。 

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参考文献

Chromophore attachment to phycobiliprotein beta-subunits: phycocyanobilin:cysteine-beta84 phycobiliprotein lyase activity of CpeS-like protein from Anabaena Sp. PCC7120
Authors: Zhao KH, Su P, Li J, Tu JM, Zhou M, Bubenzer C, Scheer H.
Journal: J Biol Chem (2006): 8573

Excitation energy transfer from phycobiliprotein to chlorophyll d in intact cells of Acaryochloris marina studied by time- and wavelength-resolved fluorescence spectroscopy
Authors: Petrasek Z, Schmitt FJ, Theiss C, Huyer J, Chen M, Larkum A, Eichler HJ, Kemnitz K, Eckert HJ.
Journal: Photochem Photobiol Sci (2005): 1016

Single-molecule spectroscopy selectively probes donor and acceptor chromophores in the phycobiliprotein allophycocyanin
Authors: Loos D, Cotlet M, De Schryver F, Habuchi S, Hofkens J.
Journal: Biophys J (2004): 2598

Evaluation of Tolypothrix germplasm for phycobiliprotein content
Authors: Prasanna R, Prasanna BM, Mohammadi SA, Singh PK.
Journal: Folia Microbiol (Praha) (2003): 59

Isolation and characterisation of phycobiliprotein rich mutant of cyanobacterium Synechocystis sp
Authors: Prasanna R, Dhar DW, Dominic TK, Tiwari ON, Singh PK.
Journal: Acta Biol Hung (2003): 113

Co-ordinated expression of phycobiliprotein operons in the chromatically adapting cyanobacterium Calothrix PCC 7601: a role for RcaD and RcaG
Authors: Noubir S, Luque I, Ochoa de Alda JA, Perewoska I, Tandeau de Marsac N, Cobley JG, Houmard J.
Journal: Mol Microbiol (2002): 749

Phycobiliprotein genes of the marine photosynthetic prokaryote Prochlorococcus: evidence for rapid evolution of genetic heterogeneity
Authors: Ting CS, Rocap G, King J, Chisholm SW.
Journal: Microbiology (2001): 3171

Novel activity of a phycobiliprotein lyase: both the attachment of phycocyanobilin and the isomerization to phycoviolobilin are catalyzed by the proteins PecE and PecF encoded by the phycoerythrocyanin operon
Authors: Zhao KH, Deng MG, Zheng M, Zhou M, Parbel A, Storf M, Meyer M, Strohmann B, Scheer H.
Journal: FEBS Lett (2000): 9

Phycobiliprotein-Fab conjugates as probes for single particle fluorescence imaging
Authors: Triantafilou K, Triantafilou M, Wilson KM.
Journal: Cytometry (2000): 226

[Phycobiliprotein and fluorescence immunological assay]
Authors: Wu P.
Journal: Sheng Li Ke Xue Jin Zhan (2000): 82

ReadiUse 预活化 APC 马来酰亚胺 [活化别藻蓝蛋白]

简要概述

产品基本信息

货号：2567

产品名称：ReadiUse 预活化 APC 马来酰亚胺 [活化别藻蓝蛋白]

规格：1mg

储存条件：2-8℃避光防潮

保质期：6个月

产品物理化学光谱特性

外观：固体

溶剂：水

激发波长（nm）：651

发射波长（nm）：660

产品介绍

ReadiUse 预活化 APC 马来酰亚胺是通过高度纯化的别藻蓝蛋白 (APC) 与 SMCC 反应制备的。 SMCC 的 NHS 基团与 APC 的赖氨酸基团反应，留下马来酰亚胺基团可与待缀合的蛋白质上的游离巯基反应。 ReadiUse 预活化 APC 马来酰亚胺经过高度纯化，可完全去除未反应的 SMCC，并冻干成粉末。 它可以立即使用，并且在与含巯基的靶分子（如还原型 IgG）混合后无需进一步制备即可偶联。 活化的别藻蓝蛋白可以很容易地与抗体和其他蛋白质结合，而无需使用添加的化学交联剂。 这些高度纯化的藻胆蛋白保持其 APC 的光谱特性。金畔生物是AAT Bioquest的中国代理商，为您提供最优质的ReadiUse 预活化 APC 马来酰亚胺 [活化别藻蓝蛋白]。 

参考文献

Chromophore attachment to phycobiliprotein beta-subunits: phycocyanobilin:cysteine-beta84 phycobiliprotein lyase activity of CpeS-like protein from Anabaena Sp. PCC7120
Authors: Zhao KH, Su P, Li J, Tu JM, Zhou M, Bubenzer C, Scheer H.
Journal: J Biol Chem (2006): 8573

Excitation energy transfer from phycobiliprotein to chlorophyll d in intact cells of Acaryochloris marina studied by time- and wavelength-resolved fluorescence spectroscopy
Authors: Petrasek Z, Schmitt FJ, Theiss C, Huyer J, Chen M, Larkum A, Eichler HJ, Kemnitz K, Eckert HJ.
Journal: Photochem Photobiol Sci (2005): 1016

Single-molecule spectroscopy selectively probes donor and acceptor chromophores in the phycobiliprotein allophycocyanin
Authors: Loos D, Cotlet M, De Schryver F, Habuchi S, Hofkens J.
Journal: Biophys J (2004): 2598

Evaluation of Tolypothrix germplasm for phycobiliprotein content
Authors: Prasanna R, Prasanna BM, Mohammadi SA, Singh PK.
Journal: Folia Microbiol (Praha) (2003): 59

Isolation and characterisation of phycobiliprotein rich mutant of cyanobacterium Synechocystis sp
Authors: Prasanna R, Dhar DW, Dominic TK, Tiwari ON, Singh PK.
Journal: Acta Biol Hung (2003): 113

Co-ordinated expression of phycobiliprotein operons in the chromatically adapting cyanobacterium Calothrix PCC 7601: a role for RcaD and RcaG
Authors: Noubir S, Luque I, Ochoa de Alda JA, Perewoska I, Tandeau de Marsac N, Cobley JG, Houmard J.
Journal: Mol Microbiol (2002): 749

Phycobiliprotein genes of the marine photosynthetic prokaryote Prochlorococcus: evidence for rapid evolution of genetic heterogeneity
Authors: Ting CS, Rocap G, King J, Chisholm SW.
Journal: Microbiology (2001): 3171

Novel activity of a phycobiliprotein lyase: both the attachment of phycocyanobilin and the isomerization to phycoviolobilin are catalyzed by the proteins PecE and PecF encoded by the phycoerythrocyanin operon
Authors: Zhao KH, Deng MG, Zheng M, Zhou M, Parbel A, Storf M, Meyer M, Strohmann B, Scheer H.
Journal: FEBS Lett (2000): 9

Phycobiliprotein-Fab conjugates as probes for single particle fluorescence imaging
Authors: Triantafilou K, Triantafilou M, Wilson KM.
Journal: Cytometry (2000): 226

[Phycobiliprotein and fluorescence immunological assay]
Authors: Wu P.
Journal: Sheng Li Ke Xue Jin Zhan (2000): 82