Texas Red TCO*单一异构体* 货号899-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。

Texas Red TCO*单一异构体*

Texas Red TCO*单一异构体*

Texas Red TCO*单一异构体*    货号899 货号 899 存储条件 在零下15度以下保存, 避免光照
规格 1 mg 价格 2472
Ex (nm) 586 Em (nm) 603
分子量 815.01 溶剂 DMSO
产品详细介绍

简要概述

产品基本信息

货号:899

产品名称:Texas Red TCO*单一异构体*

规格:1 mg

储存条件:-15℃避光防潮

保质期:12个月

 

产品物理化学光谱特性

分子量:815.01

外观:固体

溶剂:DMSO

Ex(nm):586

Em(nm):603

 

产品介绍

四嗪-反式-环辛烯(TCO)的连接构成了快速无毒的生物分子标记方法。四嗪功能化的分子与TCO功能化的分子反应,通过二氢吡嗪部分形成稳定的结合物。这种反电子需求环加成反应由于使用TCO作为亲二烯体而具有极快的环加成动力学的潜力而广受欢迎。AAT Bioquest提供了一组含四嗪和TCO的染料,用于探索可以利用这种强大点击反应的各种生物系统。Texas Red-TCO可以很容易地用于标记四嗪修饰的生物分子,用于荧光成像和其他基于荧光的生化分析。它广泛用于标记四嗪修饰的肽,蛋白质和寡核苷酸等。

点击查看光谱

 

参考文献

A Pretargeted Imaging Strategy for Immune Checkpoint Ligand PD-L1 Expression in Tumor Based on Bioorthogonal Diels-Alder Click Chemistry.
Authors: Qiu, Lin and Tan, Hui and Lin, Qingyu and Si, Zhan and Mao, Wujian and Wang, Tingting and Fu, Zhequan and Cheng, Dengfeng and Shi, Hongcheng
Journal: Molecular imaging and biology (2020): 842-853

A Systematic Study of Coumarin-Tetrazine Light-Up Probes for Bioorthogonal Fluorescence Imaging.
Authors: Galeta, Juraj and Dzijak, Rastislav and Obořil, Jan and Dračínský, Martin and Vrabel, Milan
Journal: Chemistry (Weinheim an der Bergstrasse, Germany) (2020)

Antibody Pretargeting Based on Bioorthogonal Click Chemistry for Cancer Imaging and Targeted Radionuclide Therapy.
Authors: Rondon, Aurélie and Degoul, Françoise
Journal: Bioconjugate chemistry (2020): 159-173

Cellular Delivery of Bioorthogonal Pretargeting Therapeutics in PSMA-Positive Prostate Cancer.
Authors: Hapuarachchige, Sudath and Huang, Colin T and Donnelly, Madeline C and Bařinka, Cyril and Lupold, Shawn E and Pomper, Martin G and Artemov, Dmitri
Journal: Molecular pharmaceutics (2020): 98-108

Conditionally Controlling Human TLR2 Activity via Trans-Cyclooctene Caged Ligands.
Authors: van de Graaff, Michel J and Oosenbrug, Timo and Marqvorsen, Mikkel H S and Nascimento, Clarissa R and de Geus, Mark A R and Manoury, Bénédicte and Ressing, Maaike E and van Kasteren, Sander I
Journal: Bioconjugate chemistry (2020): 1685-1692

Effect of Modulating FcRn Binding on Direct and Pretargeted Tumor Uptake of Full-length Antibodies.
Authors: Nazarova, Lidia and Rafidi, Hanine and Mandikian, Danielle and Ferl, Gregory Z and Koerber, James T and Davies, Christopher W and Ulufatu, Sheila and Ho, Jason and Lau, Jeffrey and Yu, Shang-Fan and Ernst, James and Sadowsky, Jack D and Boswell, C Andrew
Journal: Molecular cancer therapeutics (2020): 1052-1058

Fluorogenic Bifunctional trans-Cyclooctenes as Efficient Tools for Investigating Click-to-Release Kinetics.
Authors: de Geus, Mark A R and Maurits, Elmer and Sarris, Alexi J C and Hansen, Thomas and Kloet, Max S and Kamphorst, Kiki and Ten Hoeve, Wolter and Robillard, Marc S and Pannwitz, Andrea and Bonnet, Sylvestre A and Codée, Jeroen D C and Filippov, Dmitri V and Overkleeft, Herman S and van Kasteren, Sander I
Journal: Chemistry (Weinheim an der Bergstrasse, Germany) (2020)

Hybrid Imaging Agents for Pretargeting Applications Based on Fusarinine C-Proof of Concept.
Authors: Summer, Dominik and Petrik, Milos and Mayr, Sonja and Hermann, Martin and Kaeopookum, Piriya and Pfister, Joachim and Klingler, Maximilian and Rangger, Christine and Haas, Hubertus and Decristoforo, Clemens
Journal: Molecules (Basel, Switzerland) (2020)

Injectable Click Chemistry-based Bioadhesives for Accelerated Wound Closure.
Authors: Li, Shuxin and Zhou, Jun and Huang, YiHui and Roy, Joyita and Zhou, Ning and Yum, Kyungsuk and Sun, Xiankai and Tang, Liping
Journal: Acta biomaterialia (2020): 95-104

Pre-targeted Imaging of Protease Activity through In Situ Assembly of Nanoparticles.
Authors: Chen, Zixin and Chen, Min and Zhou, Kaixiang and Rao, Jianghong
Journal: Angewandte Chemie (International ed. in English) (2020): 7864-7870

说明书
Texas Red TCO*单一异构体*.pdf

Live or Dead 细胞活性检测试剂盒 *红/蓝双色荧光* 货号22788-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。

Live or Dead 细胞活性检测试剂盒 *红/蓝双色荧光*

Live or Dead 细胞活性检测试剂盒 *红/蓝双色荧光*

Live or Dead 细胞活性检测试剂盒 *红/蓝双色荧光*    货号22788 货号 22788 存储条件 在零下15度以下保存, 避免光照
规格 200 Tests 价格 3924
Ex (nm) 360; 550 Em (nm) 450; 570
分子量 溶剂
产品详细介绍

简要概述

Live or Dead 细胞活性检测试剂盒是美国AAT Bioquest生产的细胞活性检测试剂盒,AAT Bioquest的Mycolight 细菌活力测定试剂盒可在革兰氏阳性和阴性细菌中提供细菌活力的双色荧光测定。该试剂盒利用我们的绿色荧光核酸染色剂MycoLight Green和红色荧光核酸染色碘化丙啶的混合物。单独使用时,MycoLight 绿色污渍通常会标记群体中的所有细菌(活的和死的)。相比之下,碘化丙锭只穿透膜受损的细菌,当两种染料都存在时,导致MycoLight 绿色染料荧光的减少。因此,使用MycoLight 绿色和碘化丙啶的适当混合物,具有完整细胞膜的活细菌发出绿色荧光,而具有损伤膜的死亡或濒死的细菌 产生红色荧光。Mycolight 细菌活力测定试剂盒是监测细菌种群活力的强大工具,可作为细胞膜完整性的函数。可以在510-530nm(FITC滤光片)和600-660nm(德克萨斯红色滤光片光片)处以488nm处(最常见的激发光源)激发荧光测定染色的细胞。金畔生物是AAT Bioquest的中国代理商,为您提供最优质的Live or Dead 细胞活性检测试剂盒。 

 

适用仪器


荧光显微镜  
激发: Texas Red(活),Cy5滤波片(活),DAPI滤波片(死)
发射: Texas Red(活),Cy5滤波片(活),DAPI滤波片(死)
推荐孔板: 黑色透明
荧光酶标仪  
激发: 610nm,360nm
发射: 650nm,450nm
cutoff: 630,420nm
推荐孔板: 黑色透明
读取模式: 底读模式

产品说明书

样品实验方案

简要概述

1.用测试化合物制备细胞
2.添加染料加工溶液
3.在室温或37°C孵育30分钟至1小时
4.在Ex / Em = 610/650 nm(截止= 630 nm,红色)和Ex / Em = 360/450 nm(截止= 420 nm,蓝色)或在荧光显微镜下观察(Texas red/Cy5通道   活细胞),(DAPI通道  死细胞)

 

工作溶液配制

将5μL的200X Cellbrite Red(组分A)和5μL的200X Nuclear Blue DCS1(组分C)加入1mL的测定缓冲液(组分B)中并充分混合以制备染料 – 工作溶液。 该染料加工溶液在室温下稳定至少1小时。 注意:由于最佳染色条件可能因细胞类型的不同而异,因此建议单独确定组分A和C的适当浓度。有关细胞样品制备的指南,请点击查看。

 

操作步骤

1.根据标准方案准备细胞。 注意:我们用星形孢菌素(SS)在37℃下处理HeLa细胞4小时以诱导细胞凋亡。 详细信息请参见图1。

2.用100μL/孔(96孔板)或25μL/孔(384孔板)的染料加工溶液替换生长培养基。

3.将染料加工溶液板在室温或37°C孵育30分钟至1小时,避光。

4.用HHBS,PBS或您选择的缓冲液洗涤细胞两次。

5.向细胞中加入100μL/孔(96孔板)或25μL/孔(384孔板)的测定缓冲液(组分B)。

6.用荧光显微镜监测荧光信号,用德克萨斯红或Cy5过滤器检测活细胞,用DAPI过滤死细胞。 还可以使用荧光酶标仪(底部读取模式)在Ex / Em = 610 / 650nm(截止= 630nm,红色)和Ex / Em = 360 / 450nm(截止= 420nm,蓝色)下分析荧光强度。

 

数据分析

Live or Dead 细胞活性检测试剂盒 *红/蓝双色荧光*    货号22788

图1.用Live或Dead 细胞活力测定试剂盒*双荧光*(Cat#22788)标记的HeLa细胞的荧光图像。 将100,000细胞/孔/100μL的HeLa细胞在96孔黑壁/透明底板中接种过夜。 将细胞用0-1μM星形孢菌素(SS)在37℃处理4小时(A-D),或在乙醇(E)中固定,然后与染料加载溶液一起温育1小时。 荧光信号分别使用具有德克萨斯红或Cy5过滤器的荧光显微镜测量活细胞(红色)和用于坏死细胞的DAPI过滤器(蓝色)。(F)使用FlexStation®酶标仪(Molecular Devices)测量相应的荧光信号,底部读数模式为Ex / Em = 610/650(截止= 630nm,红色),Ex / Em = 360/450(截止= 分别为420nm,蓝色)。

 

参考文献

Functional imaging of neuronal activity of auditory cortex by using Cal-520 in anesthetized and awake mice
Authors: Jingcheng Li, Jianxiong Zhang, Meng Wang, Junxia Pan, Xiaowei Chen, Xiang Liao
Journal: Biomedical Optics Express (2017): 2599–2610

NINJ2–A novel regulator of endothelial inflammation and activation
Authors: Jingjing Wang, Jingjing Fa, Pengyun Wang, Xinzhen Jia, Huixin Peng, Jing Chen, Yifan Wang, Chenhui Wang, Qiuyun Chen, Xin Tu
Journal: Cellular Signalling (2017)

Erythropoietin Stimulates Endothelial Progenitor Cells to Induce Endothelialization in an Aneurysm Neck After Coil Embolization by Modulating Vascular Endothelial Growth Factor
Authors: Peixi Liu, Yingjie Zhou, Qingzhu An, Yaying Song, Xi Chen, Guo-Yuan Yang, Wei Zhu
Journal: MEDICINE (2016): 1–8

Flexible Endoscopic Spray Application of Respiratory Epithelial Cells as Platform Technology to Apply Cells in Tubular Organs
Authors: Anja Lena Thiebes, Manuel Armin Reddemann, Johannes Palmer, Reinhold Kneer, Stefan Jockenhoevel, Christian Gabriel Cornelissen
Journal: Tissue Engineering Part C: Methods (2016): 322–331

Influence of hypothermia and subsequent rewarming upon leukocyte-endothelial interactions and expression of Junctional-Adhesion-Molecules A and B
Authors: Nicolai V Bogert, Isabella Werner, Angela Kornberger, Patrick Meybohm, Anton Moritz, Till Keller, Ulrich A Stock, Andres Beiras-Fernandez
Journal: Scientific reports (2016)

Inhibition of ABC transport proteins by oil sands process affected water
Authors: Hattan A Alharbi, David MV Saunders, Ahmed Al-Mousa, Jane Alcorn, Alberto S Pereira, Jonathan W Martin, John P Giesy, Steve B Wiseman
Journal: Aquatic Toxicology (2016): 81–88

Rapid generation of collagen-based microtissues to study cell–matrix interactions
Authors: Marie-Elena Brett, Alexandra L Crampton, David K Wood
Journal: Technology (2016): 1–8

Toxicokinetics and toxicodynamics of chlorpyrifos is altered in embryos of Japanese medaka exposed to oil sands process-affected water: evidence for inhibition of P-glycoprotein
Authors: Hattan A Alharbi, Jane Alcorn, Ahmed Al-Mousa, John P Giesy, Steve B Wiseman
Journal: Journal of Applied Toxicology (2016)

Spraying respiratory epithelial cells to coat tissue-engineered constructs
Authors: Anja Lena Thiebes, Stefanie Albers, Christian Klopsch, Stefan Jockenhoevel, Christian G Cornelissen
Journal: BioResearch open access (2015): 278–287

The efficient hemostatic effect of Antarctic krill chitosan is related to its hydration property
Authors: Shuai Wu, Zhuoyao Huang, Jianhui Yue, Di Liu, Ting Wang, Pierre Ezanno, Changshun Ruan, Xiaoli Zhao, William W Lu, Haobo Pan
Journal: Carbohydrate polymers (2015): 295–303

 

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说明书
Live or Dead 细胞活性检测试剂盒 *红/蓝双色荧光*.pdf

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* 干冰运输、大包装及大批量的产品需酌情添加运输费用


* 零售价、促销产品折扣、运输费用、库存情况、产品及包装规格可能因各种原因有所变动,恕不另行通知,确切详情请联系上海金畔生物科技有限公司客服。

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* 干冰运输、大包装及大批量的产品需酌情添加运输费用


* 零售价、促销产品折扣、运输费用、库存情况、产品及包装规格可能因各种原因有所变动,恕不另行通知,确切详情请联系上海金畔生物科技有限公司客服。

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应用: IHC, IP, WB