标签归档：缓冲液

iFluor 488-PEG12-dUTP *1 mM Tris 缓冲液 (pH 7.5)* 货号17041-AAT Bioquest荧光染料

发表于由

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品，欢迎访问AAT Bioquest荧光染料官网了解更多信息。

iFluor 488-PEG12-dUTP *1 mM Tris 缓冲液 (pH 7.5)*

iFluor 488-PEG12-dUTP *1 mM Tris 缓冲液 (pH 7.5)*

iFluor 488-PEG12-dUTP *1 mM Tris 缓冲液 (pH 7.5)* 货号17041 货号 17041 存储条件 在零下15度以下保存, 避免光照
规格 25 nmoles 价格 1164
Ex (nm) 491 Em (nm) 516
分子量 2274.60 溶剂 Water
产品详细介绍

简要概述

产品基本信息

货号：17041

产品名称：iFluor 488-PEG12-dUTP *1 mM Tris 缓冲液 (pH 7.5)*

规格：25 nmoles

储存条件：-15℃避光防潮

保质期：12个月

 

产品物理化学光谱特性

分子量：2274.60

溶剂：水

激发波长(nm)：491

发射波长(nm)：516

 

产品介绍

染料修饰的脱氧尿苷 5′-三磷酸是通过常规酶促掺入方法（如逆转录、切口平移、随机引物标记或 PCR）生产染料标记 DNA 的最常用方法之一。 这种酶促荧光标记方法广泛用于 FISH 探针和基于微阵列的实验。 这种 iFluor 488-PEG12-dUTP 缀合物具有与 ChromaTide Alexa Fluor 488-dUTP 相同的光谱特性。 其 PEG12 间隔区使其更有效地通过聚合酶整合到 DNA 中。 它与 SpectrumGreen 滤光片组一起用作绿色荧光颜色。 它提供了具有高光稳定性且不受 pH 影响的明亮信号。 

点击查看光谱

 

参考文献

Mangiferin Inhibits Apoptosis in Doxorubicin-Induced Vascular Endothelial Cells via the Nrf2 Signaling Pathway.
Authors: Ismail, Mohammad Bani and Rajendran, Peramaiyan and AbuZahra, Hamad Mohammed and Veeraraghavan, Vishnu Priya
Journal: International journal of molecular sciences (2021)

Therapeutic effect of carbon monoxide-releasing molecule-3 on acute lung injury after hemorrhagic shock and resuscitation.
Authors: Kumada, Yuta and Takahashi, Toru and Shimizu, Hiroko and Nakamura, Ryu and Omori, Emiko and Inoue, Kazuyoshi and Morimatsu, Hiroshi
Journal: Experimental and therapeutic medicine (2019): 3429-3440

Activation of β-catenin signaling in aggrecan-expressing cells in temporomandibular joint causes osteoarthritis-like defects.
Authors: Hui, Tianqian and Zhou, Yachuan and Wang, Tingyu and Li, Jun and Zhang, Shanxing and Liao, Lifan and Gu, Jianhong and Ye, Ling and Zhao, Lan and Chen, Di
Journal: International journal of oral science (2018): 13

Adenovirus-delivered wwox inhibited lung cancer growth in vivo in a mouse model.
Authors: Zhou, Y and Shou, F and Zhang, H and You, Q
Journal: Cancer gene therapy (2016): 1-6

Corn silk maysin induces apoptotic cell death in PC-3 prostate cancer cells via mitochondria-dependent pathway.
Authors: Lee, Jisun and Lee, Seul and Kim, Sun-Lim and Choi, Ji Won and Seo, Jeong Yeon and Choi, Doo Jin and Park, Yong Il
Journal: Life sciences (2014): 47-55

Hepatoprotective properties of sesamin against CCl4 induced oxidative stress-mediated apoptosis in mice via JNK pathway.
Authors: Ma, Jie-Qiong and Ding, Jie and Zhang, Li and Liu, Chan-Min
Journal: Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association (2014): 41-8

Immunoliposome co-delivery of bufalin and anti-CD40 antibody adjuvant induces synergetic therapeutic efficacy against melanoma.
Authors: Li, Ying and Yuan, Jiani and Yang, Qian and Cao, Wei and Zhou, Xuanxuan and Xie, Yanhua and Tu, Honghai and Zhang, Ya and Wang, Siwang
Journal: International journal of nanomedicine (2014): 5683-700

Inhibition of APE1/Ref-1 redox activity rescues human retinal pigment epithelial cells from oxidative stress and reduces choroidal neovascularization.
Authors: Li, Y and Liu, X and Zhou, T and Kelley, M R and Edwards, P and Gao, H and Qiao, X
Journal: Redox biology (2014): 485-94

Differential expression of the P2X7 receptor in ovarian surface epithelium during the oestrous cycle in the mouse.
Authors: Vázquez-Cuevas, F G and Cruz-Rico, A and Garay, E and García-Carrancá, A and Pérez-Montiel, D and Juárez, B and Arellano, R O
Journal: Reproduction, fertility, and development (2013): 971-84

Effects of ionizing radiation on Capsicum baccatum var. pendulum (Solanaceae).
Authors: Scaldaferro, M A and Prina, A R and Moscone, E A and Kwasniewska, J
Journal: Applied radiation and isotopes : including data, instrumentation and methods for use in agriculture, industry and medicine (2013): 103-8

说明书
iFluor 488-PEG12-dUTP *1 mM Tris 缓冲液 (pH 7.5)*.pdf

ReadiUse™Tyramide（TSA）/ Styramide（PSA）优化的反应缓冲液 货号45090-AAT Bioquest荧光染料

发表于由

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品，欢迎访问AAT Bioquest荧光染料官网了解更多信息。

ReadiUse™Tyramide（TSA）/ Styramide（PSA）优化的反应缓冲液

ReadiUse™Tyramide（TSA）/ Styramide（PSA）优化的反应缓冲液

货号 45090 存储条件 在2-8度冷藏保存
规格 1000 Reactions 价格 1200
Ex (nm) Em (nm)
分子量 溶剂
产品详细介绍

简要概述

对于许多免疫组织化学（IHC）应用，传统的酶促扩增程序足以实现足够的抗原检测。但是，有几个因素限制了这些程序的敏感性和实用性。酪酰胺信号放大（TSA）已被证明是一种特别通用且功能强大的酶放大技术，具有更高的测定灵敏度。TSA基于HRP在低浓度过氧化氢存在下将标记的含酪胺底物转化为可与HRP或附近的酪氨酸残基共价结合的氧化的高反应性自由基的能力。Power Styramide™信号放大（PSA™）是对TSA系统的最新升级，具有比相应TSA试剂高10至50倍的改进荧光信号。金畔生物是AAT Bioquest的中国代理商，为您提供最优质的TSA/PSA相关产品。 

产品说明书

样品分析方案

概述

细胞或组织培养

在封闭缓冲液中添加一抗

加入结合HRP的二抗

准备Styramide™（PSA）或Tyramide（TSA）工作溶液，并在室温下在细胞或组织中涂抹5-10分钟 

 

储备溶液配制

1.PSA储备溶液（100X）

将100μLDMSO加入到iFluor™染料标记的Styramide™缀合物（例如＃45000）的小瓶中，制成100X Styramide™储备液。
注意：制成单次使用的等分试样，并将未使用的100X储备溶液在2-8°C的暗处存储，并避免重复冻融循环。

2. H2O2储备溶液

向90μL的ddH 2 O中添加10μL的3％过氧化氢（组分B）。
注意：在使用当天准备新鲜的100X H 2 O 2溶液。

 

工作溶液配制

PSA工作溶液（1X）

每1 mL PSA工作溶液需要1 mL TSA / PSA缓冲液（组分A），10μLStyramide™储备溶液和10μLH 2 O 2储备溶液。
注意：1 mL足够进行10次测试，基于每个盖玻片或96孔微孔板中每孔所需的100μLStyramide™工作溶液。
注意：Styramide™工作溶液必须在制备后2小时内使用，并避免直接暴露在光线下。

 

 

参考文献

A Protocol for Double Fluorescent In Situ Hybridization and Immunohistochemistry for the Study of Embryonic Brain Development in Tribolium castaneum.
Authors: Buescher, Marita and Oberhofer, Georg and Garcia-Perez, Natalia Carolina and Bucher, Gregor
Journal: Methods in molecular biology (Clifton, N.J.) (2020): 219-232

Automation of Multiplexed RNAscope Single-Molecule Fluorescent In Situ Hybridization and Immunohistochemistry for Spatial Tissue Mapping.
Authors: Roberts, Kenny and Bayraktar, Omer Ali
Journal: Methods in molecular biology (Clifton, N.J.) (2020): 229-244

Detection of Cytokine Receptors Using Tyramide Signal Amplification for Immunofluorescence.
Authors: Wang, Herui and Pangilinan, Ryan L and Zhu, Yan
Journal: Methods in molecular biology (Clifton, N.J.) (2020): 89-97

Estimating the ploidy of Gracilariopsis lemaneiformis at both the cellular and genomic level.
Authors: Chen, Haihong and Feng, Xiaoqing and Jiang, Minjie and Xiao, Baoheng and Zhang, Jingyu and Zhang, Wei and Hu, Yiyi and Sui, Zhenghong
Journal: Journal of phycology (2020)

Liver fibrosis and CD206+ macrophage accumulation are suppressed by anti-GM-CSF therapy.
Authors: Tan-Garcia, Alfonso and Lai, Fritz and Sheng Yeong, Joe Poh and Irac, Sergio E and Ng, Pei Y and Msallam, Rasha and Tatt Lim, Jeffrey Chun and Wai, Lu-En and Tham, Christine Y L and Choo, Su P and Lim, Tony and Young, Dan Y and D’Ambrosio, Roberta and Degasperi, Elisabetta and Perbellini, Riccardo and Newell, Evan and Le Bert, Nina and Ginhoux, Florent and Bertoletti, Antonio and Chen, Qingfeng and Dutertre, Charles-Antoine
Journal: JHEP reports : innovation in hepatology (2020): 100062

Procedural Requirements and Recommendations for Multiplex Immunofluorescence Tyramide Signal Amplification Assays to Support Translational Oncology Studies.
Authors: Parra, Edwin Roger and Jiang, Mei and Solis, Luisa and Mino, Barbara and Laberiano, Caddie and Hernandez, Sharia and Gite, Swati and Verma, Anuj and Tetzlaff, Michael and Haymaker, Cara and Tamegnon, Auriole and Rodriguez-Canales, Jaime and Hoyd, Clifford and Bernachez, Chantale and Wistuba, Ignacio
Journal: Cancers (2020)

Sensitive Multiplexed Fluorescent In Situ Hybridization Using Enhanced Tyramide Signal Amplification and Its Combination with Immunofluorescent Protein Visualization in Zebrafish.
Authors: Lauter, Gilbert and Söll, Iris and Hauptmann, Giselbert
Journal: Methods in molecular biology (Clifton, N.J.) (2020): 397-409

Tagging the vanA gene in wastewater microbial communities for cell sorting and taxonomy of vanA carrying cells.
Authors: Gallego, Sara and Barkay, Tamar and Fahrenfeld, N L
Journal: The Science of the total environment (2020): 138865

The autophagic response to Staphylococcus aureus provides an intracellular niche in neutrophils.
Authors: Prajsnar, Tomasz K and Serba, Justyna J and Dekker, Bernice M and Gibson, Josie F and Masud, Samrah and Fleming, Angeleen and Johnston, Simon A and Renshaw, Stephen A and Meijer, Annemarie H
Journal: Autophagy (2020): 1-15

A Hybrid Detection Method Based on Peroxidase-mediated Signal Amplification and Click Chemistry for Highly Sensitive Background-free Immunofluorescent Staining.
Authors: Antonov, Stanislav A and Novosadova, Ekaterina V and Kobylansky, Andrey G and Tarantul, Vyacheslav Z and Grivennikov, Igor A
Journal: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society (2019): 771-782

说明书
ReadiUse™Tyramide（TSA）/ Styramide（PSA）优化的反应缓冲液.pdf

荧光素-12-dCTP *1 mM Tris 缓冲液 (pH 7.5)* 货号17035-AAT Bioquest荧光染料

发表于由

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品，欢迎访问AAT Bioquest荧光染料官网了解更多信息。

荧光素-12-dCTP *1 mM Tris 缓冲液 (pH 7.5)*

荧光素-12-dCTP *1 mM Tris 缓冲液 (pH 7.5)*

荧光素-12-dCTP *1 mM Tris 缓冲液 (pH 7.5)* 货号17035 货号 17035 存储条件 在零下15度以下保存, 避免光照
规格 25 nmoles 价格 1164
Ex (nm) 498 Em (nm) 517
分子量 991.69 溶剂 Water
产品详细介绍

简要概述

产品基本信息

货号：17035

产品名称：荧光素-12-dCTP *1 mM Tris 缓冲液 (pH 7.5)*

规格：25 nmoles

储存条件：-15℃避光防潮

保质期：6个月

 

产品物理化学光谱特性

分子量：991.69

Ex(nm):498

Em(nm):517

外观：液体

溶剂：水

 

产品介绍

荧光素-12-dCTP 可以作为其天然对应物 dCTP 掺入 DNA 序列中，从而将荧光素标签掺入 DNA 分子中。它可用于 DNA/cDNA 的直接酶促标记，例如通过 PCR 或切口平移。所得荧光素标记的 DNA/cDNA 探针非常适合荧光杂交应用，例如 FISH 或基于微阵列的基因表达谱分析。荧光素标签和胞苷之间的接头已针对最佳底物掺入和标记效率进行了优化。

点击查看光谱

 

参考文献

Development and Preliminary Application of Multiplex Loop-Mediated Isothermal Amplification Coupled With Lateral Flow Biosensor for Detection of Mycobacterium tuberculosis Complex.
Authors: Wang, Xingyun and Wang, Guirong and Wang, Yacui and Quan, Shuting and Qi, Hui and Sun, Lin and Shen, Chen and Huang, Hairong and Jiao, Weiwei and Shen, Adong
Journal: Frontiers in cellular and infection microbiology (2021): 666492

Human Pol ζ purified with accessory subunits is active in translesion DNA synthesis and complements Pol η in cisplatin bypass.
Authors: Lee, Young-Sam and Gregory, Mark T and Yang, Wei
Journal: Proceedings of the National Academy of Sciences of the United States of America (2014): 2954-9

[Detection of heterogeneity and evolution of subclones in t(8;21) AML by QM-FISH].
Authors: Wang, Ying-chan and Hu, Lin-ping and Lin, Dong and Li, Cheng-wen and Yuan, Tian and Jia, Yu-jiao and Tian, Zheng and Tang, Ke-jing and Wang, Min and Wang, Jian-xiang
Journal: Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi (2013): 844-50

A comprehensive HBV array for the detection of HBV mutants and genotype.
Authors: Chan, Kaimin and Yam, Irene and Yuen, John and Yuen, Man-Fung and Lai, Ching-Lung and Alexander, Graeme J and Chan, Tai-Kwong and Chan, Vivian
Journal: Clinical biochemistry (2011): 1253-60

Cationic conjugated polymers for optical detection of DNA methylation, lesions, and single nucleotide polymorphisms.
Authors: Duan, Xinrui and Liu, Libing and Feng, Fude and Wang, Shu
Journal: Accounts of chemical research (2010): 260-70

Development of a universal chemiluminometric genotyping method for high-throughput detection of 7 LDLR gene mutations in Greek population.
Authors: Glynou, Kyriaki and Laios, Eleftheria and Drogari, Euridiki and Tsaoussis, Vassilis
Journal: Clinical biochemistry (2008): 335-42

High-throughput microtiter well-based chemiluminometric genotyping of 15 HBB gene mutations in a dry-reagent format.
Authors: Glynou, Kyriaki and Kastanis, Petros and Boukouvala, Sotiria and Tsaoussis, Vassilis and Ioannou, Penelope C and Christopoulos, Theodore K and Traeger-Synodinos, Joanne and Kanavakis, Emmanuel
Journal: Clinical chemistry (2007): 384-91

Methylation-dependent fragment separation: direct detection of DNA methylation by capillary electrophoresis of PCR products from bisulfite-converted genomic DNA.
Authors: Boyd, Victoria L and Moody, Kristina I and Karger, Achim E and Livak, Kenneth J and Zon, Gerald and Burns, John W
Journal: Analytical biochemistry (2006): 266-73

Quantitation of fluorescent nucleotide incorporation by capillary gel electrophoresis and laser-induced fluorescence detection.
Authors: Cruickshank, K A
Journal: Analytical biochemistry (1999): 21-31

DNA copy number changes in childhood acute lymphoblastic leukemia.
Authors: Larramendy, M L and Huhta, T and Heinonen, K and Vettenranta, K and Mahlamäki, E and Riikonen, P and Saarinen-Pihkala, U M and Knuutila, S
Journal: Haematologica (1998): 890-5

说明书
荧光素-12-dCTP *1 mM Tris 缓冲液 (pH 7.5)*.pdf

膜联蛋白结合缓冲液 *10X 针对成像和流式细胞术优化* 货号22870-AAT Bioquest荧光染料

发表于由

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品，欢迎访问AAT Bioquest荧光染料官网了解更多信息。

膜联蛋白结合缓冲液 *10X 针对成像和流式细胞术优化*

膜联蛋白结合缓冲液 *10X 针对成像和流式细胞术优化*

货号 22870 存储条件 在2-8度冷藏保存
规格 10 mL 价格 924
Ex (nm) Em (nm)
分子量 溶剂
产品详细介绍

简要概述

产品基本信息

货号：22870

产品名称：膜联蛋白结合缓冲液 *10X 针对成像和流式细胞术优化*

规格：10ml

储存条件：2-8℃避光防潮

保质期：6个月

 

产品介绍

膜联蛋白 V 结合缓冲液 * 10X 优化用于成像和流式细胞术* 是一种等渗缓冲液，用于促进使用膜联蛋白 V 偶联物标记凋亡细胞。 该缓冲液的 pH 值为 7.4，含有最佳浓度的钙，可促进膜联蛋白 V 与凋亡细胞表面暴露的磷脂酰丝氨酸残基结合。

膜联蛋白 V 结合缓冲液 *10X 优化用于成像和流式细胞术* 以 10X 浓缩液形式提供。 在膜联蛋白 V 染色之前，我们建议使用蒸馏水将 10X 浓缩液稀释为 1X 工作溶液。 实验后，丢弃任何剩余的 1X 工作溶液。金畔生物是AAT Bioquest的中国代理商，为您提供最优质的膜联蛋白结合缓冲液。 

说明书
膜联蛋白结合缓冲液 *10X 针对成像和流式细胞术优化*.pdf

荧光素-12-dATP *1 mM Tris 缓冲液 (pH 7.5)* 货号17036-AAT Bioquest荧光染料

发表于由

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品，欢迎访问AAT Bioquest荧光染料官网了解更多信息。

荧光素-12-dATP *1 mM Tris 缓冲液 (pH 7.5)*

荧光素-12-dATP *1 mM Tris 缓冲液 (pH 7.5)*

荧光素-12-dATP *1 mM Tris 缓冲液 (pH 7.5)* 货号17036 货号 17036 存储条件 在零下15度以下保存, 避免光照
规格 25 nmoles 价格 1164
Ex (nm) 498 Em (nm) 517
分子量 1014.72 溶剂 Water
产品详细介绍

简要概述

产品基本信息

货号：17036

产品名称：荧光素-12-dATP *1 mM Tris 缓冲液 (pH 7.5)*

规格：25 nmoles

储存条件：-15℃避光防潮

保质期：6个月

 

产品物理化学光谱特性

分子量：1014.72

Ex(nm):498

Em(nm):517

外观：液体

溶剂：水

 

产品介绍

荧光素-12-dATP 可能会作为其天然对应物 dATP 掺入 DNA 序列中，从而将荧光素标签掺入 DNA 分子中。它可用于 DNA/cDNA 的直接酶促标记，例如通过切口平移、随机引物、聚合酶链反应 (PCR) 和 3′ 端标记。所得荧光素标记的 DNA/cDNA 探针非常适合荧光杂交应用，例如 FISH 或基于微阵列的基因表达谱分析。荧光素标签和腺嘌呤之间的接头已针对最佳底物掺入和标记效率进行了优化。标记的产物可以通过荧光直接检测或通过与抗荧光素 HRP 或 AP 偶联物结合间接检测。

点击查看光谱

 

参考文献

Highly sensitive fluorometric determination of thrombin by on-chip signal amplification initiated by terminal deoxynucleotidyl transferase.
Authors: Wen, Dongxiao and He, Minhui and Ma, Kefeng and Cui, Ying and Kong, Jinming and Yang, Huaixia and Liu, Qingyun
Journal: Mikrochimica acta (2018): 380

说明书
荧光素-12-dATP *1 mM Tris 缓冲液 (pH 7.5)*.pdf