标签归档：缓冲液

iFluor 647-PEG12-dUTP *1 mM Tris 缓冲液 (pH 7.5)* 货号17043-AAT Bioquest荧光染料

发表于由

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品，欢迎访问AAT Bioquest荧光染料官网了解更多信息。

iFluor 647-PEG12-dUTP *1 mM Tris 缓冲液 (pH 7.5)*

iFluor 647-PEG12-dUTP *1 mM Tris 缓冲液 (pH 7.5)*

iFluor 647-PEG12-dUTP *1 mM Tris 缓冲液 (pH 7.5)* 货号17043 货号 17043 存储条件 在零下15度以下保存, 避免光照
规格 25 nmoles 价格 1164
Ex (nm) 656 Em (nm) 670
分子量 2293.72 溶剂 Water
产品详细介绍

简要概述

产品基本信息

货号：17043

产品名称：iFluor 647-PEG12-dUTP *1 mM Tris 缓冲液 (pH 7.5)*

规格：25 nmoles

储存条件：-15℃避光防潮

保质期：12个月

 

产品物理化学光谱特性

分子量：2293.72

溶剂：水

外观：液体

激发波长(nm)：656

发射波长(nm)：670

 

产品介绍

染料修饰的脱氧尿苷 5′-三磷酸是通过常规酶促掺入方法（如逆转录、切口平移、随机引物标记或 PCR）生产染料标记 DNA 的最常用方法之一。 这种酶促荧光标记方法广泛用于 FISH 探针和基于微阵列的实验。 这种 iFluor 647-PEG12-dUTP 缀合物具有与 ChromaTide Alexa Fluor 647-dUTP 相同的光谱特性。 其 PEG12 间隔区使其更有效地通过聚合酶整合到 DNA 中。 它用作带有 SpectrumFRed 滤光片组的远红色荧光颜色。 它提供了具有高光稳定性且不受 pH 影响的明亮信号。

点击查看光谱

 

参考文献

Loss of PDPK1 abrogates resistance to gemcitabine in label-retaining pancreatic cancer cells.
Authors: Li, Dandan and Mullinax, John E and Aiken, Taylor and Xin, Hongwu and Wiegand, Gordon and Anderson, Andrew and Thorgeirsson, Snorri and Avital, Itzhak and Rudloff, Udo
Journal: BMC cancer (2018): 772

dUTPs conjugated with zwitterionic Cy3 or Cy5 fluorophore analogues are effective substrates for DNA amplification and labelling by Taq polymerase.
Authors: Zasedateleva, Olga A and Vasiliskov, Vadim A and Surzhikov, Sergey A and Kuznetsova, Viktoriya E and Shershov, Valeriy E and Guseinov, Timur O and Smirnov, Igor P and Yurasov, Roman A and Spitsyn, Maksim A and Chudinov, Alexander V
Journal: Nucleic acids research (2018): e73

Mass-spectrometry analysis of modifications at DNA termini induced by DNA polymerases.
Authors: Smirnov, Igor P and Kolganova, Natalia A and Vasiliskov, Vadim A and Chudinov, Alexander V and Timofeev, Edward N
Journal: Scientific reports (2017): 6674

Separation of replication and transcription domains in nucleoli.
Authors: Smirnov, E and Borkovec, J and Kováčik, L and Svidenská, S and Schröfel, A and Skalníková, M and Švindrych, Z and Křížek, P and Ovesný, M and Hagen, G M and Juda, P and Michalová, K and Cardoso, M C and Cmarko, D and Raška, I
Journal: Journal of structural biology (2014): 259-66

A prenatally ascertained, maternally inherited 14.8 Mb duplication of chromosomal bands Xq13.2-q21.31 associated with multiple congenital abnormalities in a male fetus.
Authors: Sismani, C and Donoghue, J and Alexandrou, A and Karkaletsi, M and Christopoulou, S and Konstantinidou, A E and Livanos, P and Patsalis, P C and Velissariou, V
Journal: Gene (2013): 138-42

[Establishment of a hydrogel chip for high-throughput detection of Y chromosome microdeletions].
Authors: Li, You-Zhi and Chen, Zhi-Yao and Wang, Hui and Huang, Huan and Song, Qin-Xin and Zhou, Guo-Hua
Journal: Zhonghua nan ke xue = National journal of andrology (2012): 109-14

Selection of reliable reference genes for gene expression study in nasopharyngeal carcinoma.
Authors: Guo, Yi and Chen, Jia-xin and Yang, Shu and Fu, Xu-ping and Zhang, Zheng and Chen, Ke-he and Huang, Yan and Li, Yao and Xie, Yi and Mao, Yu-min
Journal: Acta pharmacologica Sinica (2010): 1487-94

Time-resolved three-dimensional molecular tracking in live cells.
Authors: Wells, Nathan P and Lessard, Guillaume A and Goodwin, Peter M and Phipps, Mary E and Cutler, Patrick J and Lidke, Diane S and Wilson, Bridget S and Werner, James H
Journal: Nano letters (2010): 4732-7

[Expression of COX10 in human non-obstructive azoospermia testes].
Authors: Yang, Bo and Yuan, Jian-Lin and Gao, Xiao-Kang and Wang, He and Shao, Chen and Liu, He-Liang and Chen, Bao-Qi and Qin, Rong-Liang and Shao, Guo-Xing and Kang, Fu-Xia
Journal: Zhonghua nan ke xue = National journal of andrology (2009): 599-603

[Expression of cell cycle molecules in human azoospermic testes].
Authors: Yang, Bo and Yuan, Jian-lin and Gao, Xiao-kang and Qin, Wei-jun and Liu, Fei and Shao, Chen and Liu, He-liang and Kang, Fu-xia
Journal: Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology (2009): 393-5

说明书
iFluor 647-PEG12-dUTP *1 mM Tris 缓冲液 (pH 7.5)*.pdf

Tris缓冲液（pH 7.5）中的生物素-14-dCTP * 1 mM * 货号17019-AAT Bioquest荧光染料

发表于由

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品，欢迎访问AAT Bioquest荧光染料官网了解更多信息。

Tris缓冲液（pH 7.5）中的生物素-14-dCTP * 1 mM *

Tris缓冲液（pH 7.5）中的生物素-14-dCTP * 1 mM *

Tris缓冲液（pH 7.5）中的生物素-14-dCTP * 1 mM * 货号17019 货号 17019 存储条件 在零下15度以下保存, 避免光照
规格 25 nmoles 价格 1272
Ex (nm) Em (nm)
分子量 905.79 溶剂 Water
产品详细介绍

简要概述

产品基本信息

货号：17019

产品名称：Tris缓冲液（pH 7.5）中的生物素-14-dCTP * 1 mM *

规格：25 nmoles

储存条件：-15℃避光防潮

保质期：12个月

 

产品物理化学光谱特性

分子量：905.79

溶剂：水

激发波长(nm)：N/A

发射波长(nm)：N/A

 

产品介绍

Tris缓冲液（pH 7.5）中的生物素-14-dCTP * 1 mM *是美国AAT Bioquest生产的荧光染料，生物素修饰的dCTP类似物广泛用于各种非放射性DNA标记反应，包括切口平移，随机引物标记，cDNA标记和3′-末端标记。已经显示生物素化的探针以与非生物素化的探针相同的速率和相同的程度与同源核酸杂交。亲和素和链霉亲和素可以检测到杂交的生物素化DNA探针。将生物素-14-dCTP酶促地掺入DNA / cDNA中，以替代dCTP。随后使用与辣根过氧化物酶（HRP），碱性磷酸酶（AP），荧光染料或琼脂糖/磁珠偶联的抗生蛋白链菌素检测所得的生物素标记的DNA / cDNA探针。在胞嘧啶N4位置和生物素标签之间的14原子间隔子将CTP碱基与生物素标签分开，从而确保了尼克翻译的最佳性能。对于使用Taq聚合酶进行PCR掺入实验，可以将50％Biotin-14-dCTP / 50％dCTP比率用于Nick翻译。或者，您可以在测试条件下确定最佳比率。金畔生物是AAT Bioquest的中国代理商，为您提供最优质的Tris缓冲液（pH 7.5）中的生物素-14-dCTP * 1 mM *。

 

参考文献

A visual DNA chip for simultaneous detection of hepatitis B virus, hepatitis C virus and human immunodeficiency virus type-1
Authors: Wen JK, Zhang XE, Cheng Z, Liu H, Zhou YF, Zhang ZP, Yang JH, Deng JY.
Journal: Biosens Bioelectron (2004): 685

Fluorescent DNA hybridization probe preparation using amine modification and reactive dye coupling
Authors: Cox WG, Singer VL.
Journal: Biotechniques (2004): 114

Aminomodified nucleobases: functionalized nucleoside triphosphates applicable for SELEX
Authors: Schoetzau T, Langner J, Moyroud E, Roehl I, Vonhoff S, Klussmann S.
Journal: Bioconjug Chem (2003): 919

Simple method for preparation of fluor/hapten-labeled dUTP
Authors: Nimmakayalu M, Henegariu O, Ward DC, Bray-Ward P.
Journal: Biotechniques (2000): 518

Topology of yeast RNA polymerase II subunits in transcription elongation complexes studied by photoaffinity cross-linking
Authors: Wooddell CI, Burgess RR.
Journal: Biochemistry (2000): 13405

Quantitative analysis of polymerase chain reaction products using biotinylated dUTP incorporation
Authors: Duplaa C, Couffinhal T, Labat L, Moreau C, Lamaziere JM, Bonnet J.
Journal: Anal Biochem (1993): 229

A non-radioisotopic reverse transcriptase assay using biotin-11-deoxyuridinetriphosphate on primer-immobilized microtiter plates
Authors: Urabe T, Sano K, Tanno M, Mizoguchi J, Otani M, Lee MH, Takasaki T, Kusakabe H, Imagawa DT, Nakai M.
Journal: J Virol Methods (1992): 145

Affinity isolation of active murine erythroleukemia cell chromatin: uniform distribution of ubiquitinated histone H2A between active and inactive fractions
Authors: Dawson BA, Herman T, Haas AL, Lough J.
Journal: J Cell Biochem (1991): 166

Non-radioactive labeling and detection of nucleic acids. IV. Synthesis and properties of digoxigenin-modified 2′-deoxyuridine-5′-triphosphates and a photoactivatable analog of digoxigenin (photodigoxigenin)
Authors: Muhlegger K, Huber E, von der Eltz H, Ruger R, Kessler C.
Journal: Biol Chem Hoppe Seyler (1990): 953

 

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生物素 CAS 58-85-5 Cat#3001

说明书
Tris缓冲液（pH 7.5）中的生物素-14-dCTP * 1 mM *.pdf

膜联蛋白结合缓冲液 *10X 针对成像和流式细胞术优化* 货号22870-AAT Bioquest荧光染料

发表于由

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品，欢迎访问AAT Bioquest荧光染料官网了解更多信息。

膜联蛋白结合缓冲液 *10X 针对成像和流式细胞术优化*

膜联蛋白结合缓冲液 *10X 针对成像和流式细胞术优化*

货号 22870 存储条件 在2-8度冷藏保存
规格 10 mL 价格 924
Ex (nm) Em (nm)
分子量 溶剂
产品详细介绍

简要概述

产品基本信息

货号：22870

产品名称：膜联蛋白结合缓冲液 *10X 针对成像和流式细胞术优化*

规格：10ml

储存条件：2-8℃避光防潮

保质期：6个月

 

产品介绍

膜联蛋白 V 结合缓冲液 * 10X 优化用于成像和流式细胞术* 是一种等渗缓冲液，用于促进使用膜联蛋白 V 偶联物标记凋亡细胞。 该缓冲液的 pH 值为 7.4，含有最佳浓度的钙，可促进膜联蛋白 V 与凋亡细胞表面暴露的磷脂酰丝氨酸残基结合。

膜联蛋白 V 结合缓冲液 *10X 优化用于成像和流式细胞术* 以 10X 浓缩液形式提供。 在膜联蛋白 V 染色之前，我们建议使用蒸馏水将 10X 浓缩液稀释为 1X 工作溶液。 实验后，丢弃任何剩余的 1X 工作溶液。金畔生物是AAT Bioquest的中国代理商，为您提供最优质的膜联蛋白结合缓冲液。 

说明书
膜联蛋白结合缓冲液 *10X 针对成像和流式细胞术优化*.pdf

GE 实验试剂 Hyclone PBS磷酸盐缓冲液（1x） 500mlSH30256.01B

发表于由

产品名称：GE 实验试剂 Hyclone PBS磷酸盐缓冲液（1x） 500ml

产品型号：SH30256.01B

产品报价：18

产品特点：GE 实验试剂 Hyclone PBS磷酸盐缓冲液（1x） 500ml，PH值：7.0-7.2，0.0067M（PO4），不含钙、镁，0.1微米无菌过滤。

SH30256.01BGE 实验试剂 Hyclone PBS磷酸盐缓冲液（1x） 500ml的详细资料：

Hyclone磷酸盐缓冲液（1x） SH30256.01B

PH值：7.0-7.2
0.0067M（PO4）
不含钙、镁 0.1微米无菌过滤
液体培养基在使用的过程中要注意以下问题：
运输中要避光冷藏，瓶口保持向上，切勿剧烈振荡；
开瓶后不要长时间暴露在空气和强光下，以保持培养基的稳定性；
开瓶后能在一周内用完，短时间内用不完可分装冷藏保存，但PH值会在随后的时间里慢慢变碱，属于正常现象，请放心使用；
所有液体培养基均经100纳米无菌过滤和出厂严格检测，除非需要，请不要私自进行二次过滤和任何形式的无菌检测。

ReadiUse 病毒RNA裂解缓冲液 货号60016-AAT Bioquest荧光染料

发表于由

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品，欢迎访问AAT Bioquest荧光染料官网了解更多信息。

ReadiUse 病毒RNA裂解缓冲液

ReadiUse 病毒RNA裂解缓冲液

货号 60016 存储条件 在2-8度冷藏保存, 避免光照
规格 500 ML 价格 11988
Ex (nm) Em (nm)
分子量 溶剂 Water
产品详细介绍

简要概述

产品基本信息

货号：60016

产品名称：ReadiUse 病毒RNA裂解缓冲液

规格：500ml

储存条件：-15℃避光防潮

保质期：24个月

 

产品介绍

目前对SARS-CoV-2的RT-PCR测试需求导致了全球范围内RNA提取试剂盒的短缺。提取试剂盒制造商和医院所需的关键缺失组分是RNA裂解缓冲液。这种RediUse 病毒RNA裂解缓冲液可以用作Roche MagNA Pure 96外部裂解缓冲液（#：06374913001），BiomérieuxNuclisens裂解缓冲液（#：280134）和Qiagen Buffer RNAeasy裂解缓冲液（#：79216）的替代品。金畔生物是AAT Bioquest的中国代理商，为您提供最优质的ReadiUse 病毒RNA裂解缓冲液。 

 

参考文献

Cell Lysis Based on an Oscillating Microbubble Array.
Authors: Liu, Xiufang and Li, Jinyuan and Zhang, Liangyu and Huang, Xiaowei and Farooq, Umar and Pang, Na and Zhou, Wei and Qi, Lin and Xu, Lisheng and Niu, Lili and Meng, Long
Journal: Micromachines (2020)

Lysis of a Lactococcus lactis Dipeptidase Mutant and Rescue by Mutation in the Pleiotropic Regulator CodY.
Authors: Huang, Chenxi and Hernandez-Valdes, Jhonatan A and Kuipers, Oscar P and Kok, Jan
Journal: Applied and environmental microbiology (2020)

PTPN21-CDSlong isoform inhibits the response of acute lymphoblastic leukemia cells to NK-mediated lysis via the KIR/HLA-I axis.
Authors: Wang, Huafang and Zhu, Ni and Ye, Xiaohang and Wang, Limengmeng and Wang, Binsheng and Shan, Wei and Lai, Xiaoyu and Tan, Yamin and Fu, Shan and Xiao, Haowen and Huang, He
Journal: Journal of cellular biochemistry (2020): 3298-3312

Schistosoma mansoni glyceraldehyde-3-phosphate dehydrogenase enhances formation of the blood-clot lysis protein plasmin.
Authors: Pirovich, David B and Da’dara, Akram A and Skelly, Patrick J
Journal: Biology open (2020)

Comparison of the efficiency of different cell lysis methods and different commercial methods for RNA extraction from Candida albicans stored in RNAlater.
Authors: Rodríguez, Antonio and Vaneechoutte, Mario
Journal: BMC microbiology (2019): 94

Epithelial ovarian cancer stem‑like cells are resistant to the cellular lysis of cytokine‑induced killer cells via HIF1A‑mediated downregulation of ICAM‑1.
Authors: Bu, Shixia and Li, Boning and Wang, Qian and Gu, Tingting and Dong, Qianggang and Miao, Xiaofei and Lai, Dongmei
Journal: International journal of oncology (2019): 179-190

Extended direct lysis method for virus detection on berries including droplet digital RT-PCR or real time RT-PCR with reduced influence from inhibitors.
Authors: Sun, Baojian and Bosch, Albert and Myrmel, Mette
Journal: Journal of virological methods (2019): 113638

High-resolution studies of lysis-lysogeny decision-making in bacteriophage lambda.
Authors: Shao, Qiuyan and Trinh, Jimmy T and Zeng, Lanying
Journal: The Journal of biological chemistry (2019): 3343-3349

Transcriptomic responses of the marine cyanobacterium Prochlorococcus to viral lysis products.
Authors: Fang, Xiaoting and Liu, Yaxin and Zhao, Yao and Chen, Yue and Liu, Riyue and Qin, Qi-Long and Li, Gang and Zhang, Yu-Zhong and Chan, Wan and Hess, Wolfgang R and Zeng, Qinglu
Journal: Environmental microbiology (2019): 2015-2028

scFTD-seq: freeze-thaw lysis based, portable approach toward highly distributed single-cell 3′ mRNA profiling.
Authors: Dura, Burak and Choi, Jin-Young and Zhang, Kerou and Damsky, William and Thakral, Durga and Bosenberg, Marcus and Craft, Joe and Fan, Rong
Journal: Nucleic acids research (2019): e16

说明书
ReadiUse 病毒RNA裂解缓冲液.pdf