Cy3-dUTP*在Tris缓冲液(pH 7.5)中为1 mM * 货号17025-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。

Cy3-dUTP*在Tris缓冲液(pH 7.5)中为1 mM *

Cy3-dUTP*在Tris缓冲液(pH 7.5)中为1 mM *

Cy3-dUTP*在Tris缓冲液(pH 7.5)中为1 mM *    货号17025 货号 17025 存储条件 在零下15度以下保存, 避免光照
规格 25 nmoles 价格 1200
Ex (nm) 555 Em (nm) 569
分子量 1150.00 溶剂 Water
产品详细介绍

简要概述

产品基本信息

货号:17025

产品名称:Cy3-dUTP*在Tris缓冲液(pH 7.5)中为1 mM *

规格:25nmoles

储存条件:-15℃避光防潮

保质期:6个月

 

产品物理化学光谱特性

分子量:1150.00

外观:溶液

溶剂:水

激发波长(nm):555

发射波长(nm):569

 

产品介绍

染料修饰的脱氧尿苷5′-三磷酸酯(例如氨基烯丙基-dUTP)可用于通过常规酶掺入方法(例如逆转录,缺口翻译,随机引物标记或PCR)生产含染料的DNA。这种酶促荧光标记方法广泛用于FISH探针和基于微阵列的实验。此Cy3-dUTP共轭物可与Spectrum Orange™滤光片组一起用作红色荧光色(Spectrum Orange™是Vysis的商标)。它在化学上与Cyanine 3-dUTP(Perkin Elmer’s NEL578001EA)相同。金畔生物是AAT Bioquest的中国代理商,为您提供最优质的Cy3系列探针。 

点击查看光谱

 

参考文献

A Fluorescent DNA Hydrogel Aptasensor Based on the Self-Assembly of Rolling Circle Amplification Products for Sensitive Detection of Ochratoxin A.
Authors: Hao, Liling and Wang, Wei and Shen, Xueqing and Wang, Shuliu and Li, Qian and An, Faliang and Wu, Shijia
Journal: Journal of agricultural and food chemistry (2020): 369-375

Engineering nicking enzymes that preferentially nick 5-methylcytosine-modified DNA.
Authors: Gutjahr, Alice and Xu, Shuang-yong
Journal: Nucleic acids research (2014): e77

Digital detection of multiple minority mutants in stool DNA for noninvasive colorectal cancer diagnosis.
Authors: Deng, Lili and Qi, Zongtai and Zou, Binjie and Wu, Haiping and Huang, Huan and Kajiyama, Tomoharu and Kambara, Hideki and Zhou, Guohua
Journal: Analytical chemistry (2012): 5645-52

Digital analysis of the expression levels of multiple colorectal cancer-related genes by multiplexed digital-PCR coupled with hydrogel bead-array.
Authors: Qi, Zongtai and Ma, Yinjiao and Deng, Lili and Wu, Haiping and Zhou, Guohua and Kajiyama, Tomoharu and Kambara, Hideki
Journal: The Analyst (2011): 2252-9

Microisolation and microcloning of bovine X-chromosomes for identification of sorted buffalo (Bubalus bubalis) spermatozoa.
Authors: Zhuang, Xin-jie and Lu, Yang-qing and Zhang, Ming and Lu, Sheng-sheng and Lu, Ke-huan
Journal: Animal reproduction science (2011): 32-6

Selection of reliable reference genes for gene expression study in nasopharyngeal carcinoma.
Authors: Guo, Yi and Chen, Jia-xin and Yang, Shu and Fu, Xu-ping and Zhang, Zheng and Chen, Ke-he and Huang, Yan and Li, Yao and Xie, Yi and Mao, Yu-min
Journal: Acta pharmacologica Sinica (2010): 1487-94

A technical note on quantum dots for multi-color fluorescence in situ hybridization.
Authors: Müller, S and Cremer, M and Neusser, M and Grasser, F and Cremer, T
Journal: Cytogenetic and genome research (2009): 351-9

[Expression of COX10 in human non-obstructive azoospermia testes].
Authors: Yang, Bo and Yuan, Jian-Lin and Gao, Xiao-Kang and Wang, He and Shao, Chen and Liu, He-Liang and Chen, Bao-Qi and Qin, Rong-Liang and Shao, Guo-Xing and Kang, Fu-Xia
Journal: Zhonghua nan ke xue = National journal of andrology (2009): 599-603

[Expression of cell cycle molecules in human azoospermic testes].
Authors: Yang, Bo and Yuan, Jian-lin and Gao, Xiao-kang and Qin, Wei-jun and Liu, Fei and Shao, Chen and Liu, He-liang and Kang, Fu-xia
Journal: Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology (2009): 393-5

A new combination of RNA-mediated DNA ligation and on-chip elongation for detecting viral RNA.
Authors: Li, TongXiang and Xie, ZhongKui and Wang, YaJun and Li, Bo and Zhang, YuBo and An, LiZhe
Journal: Diagnostic microbiology and infectious disease (2008): 26-33

说明书
Cy3-dUTP*在Tris缓冲液(pH 7.5)中为1 mM *.pdf

膜联蛋白结合缓冲液 *10X 针对成像和流式细胞术优化* 货号22871-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。

膜联蛋白结合缓冲液 *10X 针对成像和流式细胞术优化*

膜联蛋白结合缓冲液 *10X 针对成像和流式细胞术优化*

货号 22871 存储条件 在2-8度冷藏保存
规格 100 mL 价格 1164
Ex (nm) Em (nm)
分子量 溶剂
产品详细介绍

简要概述

产品基本信息

货号:22871

产品名称:膜联蛋白结合缓冲液 *10X 针对成像和流式细胞术优化*

规格:100ml

储存条件:2-8℃避光防潮

保质期:6个月

 

产品介绍

膜联蛋白 V 结合缓冲液 * 10X 优化用于成像和流式细胞术* 是一种等渗缓冲液,用于促进使用膜联蛋白 V 偶联物标记凋亡细胞。 该缓冲液的 pH 值为 7.4,含有最佳浓度的钙,可促进膜联蛋白 V 与凋亡细胞表面暴露的磷脂酰丝氨酸残基结合。

膜联蛋白 V 结合缓冲液 *10X 优化用于成像和流式细胞术* 以 10X 浓缩液形式提供。 在膜联蛋白 V 染色之前,我们建议使用蒸馏水将 10X 浓缩液稀释为 1X 工作溶液。 实验后,丢弃任何剩余的 1X 工作溶液。金畔生物是AAT Bioquest的中国代理商,为您提供最优质的膜联蛋白结合缓冲液。 

说明书
膜联蛋白结合缓冲液 *10X 针对成像和流式细胞术优化*.pdf

CytoWatch 多路复用缓冲液*流式细胞仪* *100X * 货号37011-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。

CytoWatch 多路复用缓冲液*流式细胞仪* *100X *

CytoWatch 多路复用缓冲液*流式细胞仪* *100X *

货号 37011 存储条件 在零下15度以下保存, 避免光照
规格 1000 Tests 价格 3612
Ex (nm) Em (nm)
分子量 溶剂 Water
产品详细介绍

简要概述

产品基本信息

货号:37011

产品名称:CytoWatch 多路复用缓冲液*流式细胞仪* *100X *

规格:1000 Tests

储存条件:-15℃避光防潮

保质期:12个月

 

产品物理化学光谱特性

外观:液体

溶剂:水

 

产品介绍

荧光抗体缀合物在紧密接触时趋于聚集,由于FRET和不同缀合物之间的其他能量转移现象,扭曲了多色染色细胞的分析结果,使荧光染料相互作用会导致染色伪影,从而让数据复杂化。 CytoWatch 多路复用缓冲液是为细胞的多色流式细胞分析而研发的缓冲液。 CytoWatch多路复用缓冲液是一种浓缩溶液,可在染色细胞之前直接添加到某些荧光抗体缀合物或其他荧光试剂的染色溶液中。 它可用于增强或改进利用两种或更多种不同染色试剂(例如,与BV染料或Qdot缀合的抗体)的多色流式细胞术实验。

 

参考文献

Adsorption of Remazol Brilliant Violet-5R Textile Dye from Aqueous Solutions by Using Eggshell Waste Biosorbent.
Authors: Rápó, Eszter and Aradi, László Előd and Szabó, Ábel and Posta, Katalin and Szép, Robert and Tonk, Szende
Journal: Scientific reports (2020): 8385

Azo dyes decolorization under high alkalinity and salinity conditions by Halomonas sp. in batch and packed bed reactor.
Authors: Montañez-Barragán, B and Sanz-Martín, J L and Gutiérrez-Macías, P and Morato-Cerro, A and Rodríguez-Vázquez, R and Barragán-Huerta, B E
Journal: Extremophiles : life under extreme conditions (2020): 239-247

Process optimization and filtration performance of an anaerobic dynamic membrane bioreactor treating textile wastewaters.
Authors: Yurtsever, Adem and Basaran, Erkan and Ucar, Deniz
Journal: Journal of environmental management (2020): 111114

Adsorptive Removal of Remazol Brilliant Violet-5R Dye from Aqueous Solutions using Calcined Eggshell as Biosorbent.
Authors: Rápó, Eszter and Posta, Katalin and Suciu, Maria and Szép, Robert and Tonk, Szende
Journal: Acta chimica Slovenica (2019): 648-658

Diet-induced microbial autofluorescence confounds flow cytometry of ex vivo isolated fecal microbes.
Authors: Denu, Lidiya and Lubin, Jean-Bernard and Douglas, Bonnie and Tuluc, Florin and Silverman, Michael A
Journal: European journal of immunology (2019): 2252-2254

Fluorochrome choices for multi-color flow cytometry.
Authors: Flores-Montero, Juan and Kalina, Tomas and Corral-Mateos, Alba and Sanoja-Flores, Luzalba and Pérez-Andrés, Martin and Martin-Ayuso, Marta and Sedek, Lukasz and Rejlova, Katerina and Mayado, Andrea and Fernández, Paula and van der Velden, Vincent and Bottcher, Sebastian and van Dongen, Jaques J M and Orfao, Alberto
Journal: Journal of immunological methods (2019): 112618

Remediation of complex remazol effluent using biochar derived from green seaweed biomass.
Authors: Gokulan, Ravindiran and Prabhu, Ganapathy Ganesh and Jegan, Josephraj
Journal: International journal of phytoremediation (2019): 1179-1189

Simultaneous Polychromatic Immunofluorescent Staining of Tissue Sections and Consecutive Imaging of up to Seven Parameters by Standard Confocal Microscopy.
Authors: Schmidt, Alfonso J and Mayer, Johannes U and Wallace, Paul K and Ronchese, Franca and Price, Kylie M
Journal: Current protocols in cytometry (2019): e64

A protocol for generating induced T cells by reprogramming B cells in vivo.
Authors: Weng, Qitong and Hu, Fangxiao and Zhang, Mengyun and Dong, Yong and Lv, Cui and Wang, Ying and Liu, Xiaofei and Wang, Jinyong
Journal: Cell regeneration (London, England) (2018): 7-15

Adsorption of basic and reactive dyes from aqueous solution onto Intsia bijuga sawdust-based activated carbon: batch and column study.
Authors: Khasri, Azduwin and Ahmad, Mohd Azmier
Journal: Environmental science and pollution research international (2018): 31508-31519

说明书
CytoWatch 多路复用缓冲液*流式细胞仪* *100X *.pdf

iFluor 555-PEG12-dUTP *1 mM Tris 缓冲液 (pH 7.5)* 货号17042-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。

iFluor 555-PEG12-dUTP *1 mM Tris 缓冲液 (pH 7.5)*

iFluor 555-PEG12-dUTP *1 mM Tris 缓冲液 (pH 7.5)*

iFluor 555-PEG12-dUTP *1 mM Tris 缓冲液 (pH 7.5)*    货号17042 货号 17042 存储条件 在零下15度以下保存, 避免光照
规格 25 nmoles 价格 1164
Ex (nm) 557 Em (nm) 570
分子量 2454.14 溶剂 Water
产品详细介绍

简要概述

产品基本信息

货号:17042

产品名称:iFluor 555-PEG12-dUTP *1 mM Tris 缓冲液 (pH 7.5)*

规格:25 nmoles

储存条件:-15℃避光防潮

保质期:12个月

 

产品物理化学光谱特性

分子量:2454.14

溶剂:水

外观:液体

激发波长(nm):557

发射波长(nm):570

 

产品介绍

染料修饰的脱氧尿苷 5′-三磷酸是通过常规酶促掺入方法(如逆转录、切口平移、随机引物标记或 PCR)生产染料标记 DNA 的最常用方法之一。 这种酶促荧光标记方法广泛用于 FISH 探针和基于微阵列的实验。 这种 iFluor 555-PEG12-dUTP 缀合物具有与 ChromaTide Alexa Fluor 555-dUTP 相同的光谱特性。 其 PEG12 间隔区使其更有效地通过聚合酶整合到 DNA 中。 它用作带有 SpectrumOrange 滤光片组的红色荧光色。 它提供了具有高光稳定性且不受 pH 影响的明亮信号。

点击查看光谱

 

参考文献

A Fluorescent DNA Hydrogel Aptasensor Based on the Self-Assembly of Rolling Circle Amplification Products for Sensitive Detection of Ochratoxin A.
Authors: Hao, Liling and Wang, Wei and Shen, Xueqing and Wang, Shuliu and Li, Qian and An, Faliang and Wu, Shijia
Journal: Journal of agricultural and food chemistry (2020): 369-375

Engineering nicking enzymes that preferentially nick 5-methylcytosine-modified DNA.
Authors: Gutjahr, Alice and Xu, Shuang-yong
Journal: Nucleic acids research (2014): e77

Digital detection of multiple minority mutants in stool DNA for noninvasive colorectal cancer diagnosis.
Authors: Deng, Lili and Qi, Zongtai and Zou, Binjie and Wu, Haiping and Huang, Huan and Kajiyama, Tomoharu and Kambara, Hideki and Zhou, Guohua
Journal: Analytical chemistry (2012): 5645-52

Digital analysis of the expression levels of multiple colorectal cancer-related genes by multiplexed digital-PCR coupled with hydrogel bead-array.
Authors: Qi, Zongtai and Ma, Yinjiao and Deng, Lili and Wu, Haiping and Zhou, Guohua and Kajiyama, Tomoharu and Kambara, Hideki
Journal: The Analyst (2011): 2252-9

Microisolation and microcloning of bovine X-chromosomes for identification of sorted buffalo (Bubalus bubalis) spermatozoa.
Authors: Zhuang, Xin-jie and Lu, Yang-qing and Zhang, Ming and Lu, Sheng-sheng and Lu, Ke-huan
Journal: Animal reproduction science (2011): 32-6

Selection of reliable reference genes for gene expression study in nasopharyngeal carcinoma.
Authors: Guo, Yi and Chen, Jia-xin and Yang, Shu and Fu, Xu-ping and Zhang, Zheng and Chen, Ke-he and Huang, Yan and Li, Yao and Xie, Yi and Mao, Yu-min
Journal: Acta pharmacologica Sinica (2010): 1487-94

A technical note on quantum dots for multi-color fluorescence in situ hybridization.
Authors: Müller, S and Cremer, M and Neusser, M and Grasser, F and Cremer, T
Journal: Cytogenetic and genome research (2009): 351-9

[Expression of COX10 in human non-obstructive azoospermia testes].
Authors: Yang, Bo and Yuan, Jian-Lin and Gao, Xiao-Kang and Wang, He and Shao, Chen and Liu, He-Liang and Chen, Bao-Qi and Qin, Rong-Liang and Shao, Guo-Xing and Kang, Fu-Xia
Journal: Zhonghua nan ke xue = National journal of andrology (2009): 599-603

[Expression of cell cycle molecules in human azoospermic testes].
Authors: Yang, Bo and Yuan, Jian-lin and Gao, Xiao-kang and Qin, Wei-jun and Liu, Fei and Shao, Chen and Liu, He-liang and Kang, Fu-xia
Journal: Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology (2009): 393-5

A new combination of RNA-mediated DNA ligation and on-chip elongation for detecting viral RNA.
Authors: Li, TongXiang and Xie, ZhongKui and Wang, YaJun and Li, Bo and Zhang, YuBo and An, LiZhe
Journal: Diagnostic microbiology and infectious disease (2008): 26-33

说明书
iFluor 555-PEG12-dUTP *1 mM Tris 缓冲液 (pH 7.5)*.pdf

Screen Quest 10X细胞染色缓冲液 *含酚红* 货号36300-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。

Screen Quest 10X细胞染色缓冲液 *含酚红*

Screen Quest 10X细胞染色缓冲液 *含酚红*

Screen Quest 10X细胞染色缓冲液 *含酚红*    货号36300 货号 36300 存储条件 在零下15度以下保存, 避免光照
规格 10 mL 价格 1272
Ex (nm) Em (nm)
分子量 溶剂
产品详细介绍

简要概述

产品基本信息

货号:36300

产品名称:Screen Quest 10X细胞染色缓冲液

规格:10ml

储存条件:-15℃避光防潮

保质期:24个月

 

产品介绍

即用型缓冲液,针对荧光细胞成像进行了优化。 在某些情况下,此缓冲液可明显增强成像信号。金畔生物是AAT Bioquest的中国代理商,为您提供最优质的Screen Quest 10X细胞染色缓冲液。 

 

图示

Screen Quest 10X细胞染色缓冲液 *含酚红*    货号36300

图1.带有Phenol Red Plus 的Screen Quest 10X细胞染色缓冲液的图

Screen Quest 10X细胞染色缓冲液 *含酚红*    货号36300

图2.使用Cal-520 AM在CHO-M1细胞中测量了ATP剂量反应。 将CHO-M1细胞以50,000个细胞/ 100 µL /孔在黑色96孔板中过夜接种。 加入含1X Phenol Red Plus 细胞染色缓冲液的HH缓冲液中的100 µL 10ug / ml Cal-520 AM,并在37oC下孵育60分钟。 加入ATP(50µL /孔)以达到最终指示的浓度。

 

参考文献

Kinetic characterization of novel NR2B antagonists using fluorescence detection of calcium flux
Authors: Bednar B, Cunningham ME, Kiss L, Cheng G, McCauley JA, Liverton NJ, Koblan KS.
Journal: J Neurosci Methods (2004): 247

Novel fluo-4 analogs for fluorescent calcium measurements
Authors: Martin VV, Beierlein M, Morgan JL, Rothe A, Gee KR.
Journal: Cell Calcium (2004): 509

Amplitude distribution of calcium sparks in confocal images: theory and studies with an automatic detection method
Authors: Cheng H, Song LS, Shirokova N, Gonzalez A, Lakatta EG, Rios E, Stern MD.
Journal: Biophys J (1999): 606

Flow cytometric kinetic assay of calcium mobilization in whole blood platelets using Fluo-3 and CD41
Authors: do Ceu Monteiro M, Sansonetty F, Goncalves MJ, O’Connor JE.
Journal: Cytometry (1999): 302

A simple numerical model of calcium spark formation and detection in cardiac myocytes
Authors: Smith GD, Keizer JE, Stern MD, Lederer WJ, Cheng H.
Journal: Biophys J (1998): 15

Monitoring calcium in outer hair cells with confocal microscopy and fluorescence ratios of fluo-3 and fura-red
Authors: Su ZL, Li N, Sun YR, Yang J, Wang IM, Jiang SC.
Journal: Shi Yan Sheng Wu Xue Bao (1998): 323

Loading and localization of Fluo-3 and Fluo-3/AM calcium indicators in sinapis alba root tissue
Authors: Tretyn A, Kado RT, Kendrick RE.
Journal: Folia Histochem Cytobiol (1997): 41

Nucleoplasmic and cytoplasmic differences in the fluorescence properties of the calcium indicator Fluo-3
Authors: Perez-Terzic C, Stehno-Bittel L, Clapham DE.
Journal: Cell Calcium (1997): 275

Detection of a trigger zone of bradykinin-induced fast calcium waves in PC12 neurites
Authors: Reber BF, Schindelholz B.
Journal: Pflugers Arch (1996): 893

Improved four-color flow cytometry method using fluo-3 and triple immunofluorescence for analysis of intracellular calcium ion ([Ca2+]i) fluxes among mouse lymph node B- and T-lymphocyte subsets
Authors: Greimers R, Trebak M, Moutschen M, Jacobs N, Boniver J.
Journal: Cytometry (1996): 205

说明书
Screen Quest 10X细胞染色缓冲液 *含酚红*.pdf