Cell Meter Caspase 8活性细胞结合检测试剂盒 红色荧光
 |
货号 |
20116 |
存储条件 |
在零下15度以下保存, 避免光照 |
| 规格 |
25 Tests |
价格 |
4368 |
| Ex (nm) |
|
Em (nm) |
|
| 分子量 |
|
溶剂 |
|
| 产品详细介绍 |
简要概述
产品基本信息
货号：20116
产品名称：Cell Meter Caspase 8活性细胞结合检测试剂盒 红色荧光
规格：25 Tests
储存条件：保存在冰箱-15℃干燥
保质期：12个月
试剂盒成分
| 成分A：iFluor 647-LETD-FMK |
1 vial |
| 成分B：洗涤缓冲液 |
1瓶（100ml） |
| 成分C：500X Nuclear Green DCS1 |
1 vial（100 µL） |
| 成分D：500X Hoechst |
1 vial（100 µL） |
适用仪器
| 流式细胞仪 |
|
| 激发： |
640 nm激光 |
| 发射： |
660/20 nm滤波片 |
| 滤波片： |
APC滤波片组 |
| 荧光显微镜 |
|
| 激发： |
Cy5滤波片 |
| 发射： |
Cy5滤波片 |
| 推荐孔板： |
黑色透明 |
| 荧光酶标仪 |
|
| 激发： |
640nm |
| 发射： |
680nm |
| cutoff： |
665nm |
| 推荐孔板： |
黑色透明 |
| 读取模式： |
底读模式 |
产品介绍
我们的Cell Meter 活细胞胱天蛋白酶活性测定试剂盒基于胱天蛋白酶的荧光FMK抑制剂。这些抑制剂是细胞可渗透的和无细胞毒性的。一旦进入细胞，胱天蛋白酶抑制剂就与活性胱天蛋白酶共价结合。此Cell Meter Caspase 8活性细胞结合检测试剂盒旨在通过测量活细胞中的caspase 8活化来检测细胞凋亡。它用于定量凋亡细胞中激活的caspase 8活性，或用于筛选caspase 8抑制剂。iFluor 647-LETD-FMK，红色标记试剂，可通过荧光显微镜，流式细胞仪或荧光酶标仪直接检测凋亡细胞中活化的胱天蛋白酶8。该试剂盒提供所有必需成分，并具有优化的测定方案。金畔生物是AAT Bioquest的中国代理商，为您提供最优质的Cell Meter Caspase 8活性细胞结合检测试剂盒。
图示
图1.在Jurkat细胞中使用Cell Meter 活细胞Caspase 8检测试剂盒对活性caspase 8进行流式细胞分析。细胞用1μM星形孢菌素处理5小时（绿色），而未处理的细胞用作对照（红色）。星形孢菌素反应被显示为蓝色的Z-VAD-FMK（胱天蛋白酶抑制剂）抑制。将细胞与iFluor 647-LETD-FMK在室温下孵育1小时。使用带有660/20 nm滤光片（APC通道）的NovoCyte流式细胞仪测量荧光强度。
|
参考文献
A matter of life and death for caspase 8.
Authors: Willson, Joseph
Journal: Nature reviews. Molecular cell biology (2020): 63
Cancer Cells Employ Nuclear Caspase-8 to Overcome the p53-Dependent G2/M Checkpoint through Cleavage of USP28.
Authors: Müller, Ines and Strozyk, Elwira and Schindler, Sebastian and Beissert, Stefan and Oo, Htoo Zarni and Sauter, Thomas and Lucarelli, Philippe and Raeth, Sebastian and Hausser, Angelika and Al Nakouzi, Nader and Fazli, Ladan and Gleave, Martin E and Liu, He and Simon, Hans-Uwe and Walczak, Henning and Green, Douglas R and Bartek, Jiri and Daugaard, Mads and Kulms, Dagmar
Journal: Molecular cell (2020): 970-984.e7
Caspase-8 Induces Lysosome-Associated Cell Death in Cancer Cells.
Authors: Zhong, Benfu and Liu, Miao and Bai, Changsen and Ruan, Yuxia and Wang, Yuanyuan and Qiu, Li and Hong, Yang and Wang, Xin and Li, Lifang and Li, Binghui
Journal: Molecular therapy : the journal of the American Society of Gene Therapy (2020)
Caspase-8: The double-edged sword.
Authors: Mandal, Ranadip and Barrón, Joan Compte and Kostova, Izabela and Becker, Sven and Strebhardt, Klaus
Journal: Biochimica et biophysica acta. Reviews on cancer (2020): 188357
Chrm3 protects against acinar cell necrosis by stabilizing caspase-8 expression in severe acute pancreatitis mice model.
Authors: Huang, Ning and Murtaza, Ghulam and Wang, Lujing and Luan, Jing and Wang, Xinlei and Sun, Yumiao and Wu, Xing and Tao, Yuxi and Shi, Shuoxi and Cao, Peihua and Qiao, Yu and Han, Dong and Kou, Jiayuan and Ma, Ning and Gao, Xu
Journal: Journal of cellular biochemistry (2020): 2618-2631
Cigarette smoke inhibits the NLRP3 inflammasome and leads to caspase-1 activation via the TLR4-TRIF-caspase-8 axis in human macrophages.
Authors: Buscetta, Marco and Di Vincenzo, Serena and Miele, Monica and Badami, Ester and Pace, Elisabetta and Cipollina, Chiara
Journal: FASEB journal : official publication of the Federation of American Societies for Experimental Biology (2020): 1819-1832
Dissecting DISC regulation via pharmacological targeting of caspase-8/c-FLIPL heterodimer.
Authors: Hillert, Laura K and Ivanisenko, Nikita V and Busse, Denise and Espe, Johannes and König, Corinna and Peltek, Sergey E and Kolchanov, Nikolai A and Ivanisenko, Vladimir A and Lavrik, Inna N
Journal: Cell death and differentiation (2020)
Edwardsiella piscicida type III protein EseJ suppresses apoptosis through down regulating type 1 fimbriae, which stimulate the cleavage of caspase-8.
Authors: He, Tian Tian and Zhou, Ying and Liu, Ying Li and Li, Duan You and Nie, Pin and Li, Ai Hua and Xie, Hai Xia
Journal: Cellular microbiology (2020): e13193
Expression levels of EPHB4, EFNB2 and caspase-8 are associated with clinicopathological features and progression of esophageal squamous cell cancer.
Authors: Ni, Qianzhi and Chen, Pingping and Zhu, Bing and Li, Jingjing and Xie, Dong and Ma, Xingyuan
Journal: Oncology letters (2020): 917-929
High-Concentrate Feeding to Dairy Cows Induces Apoptosis via the NOD1/Caspase-8 Pathway in Mammary Epithelial Cells.
Authors: Ul Aabdin, Zain and Cheng, Xiaoye and Dai, Hongyu and Wang, Yan and Sahito, Benazir and Roy, Animesh Chandra and Memon, Meena Arif and Shen, Xiangzhen
Journal: Genes (2020)
说明书
Cell Meter Caspase 8活性细胞结合检测试剂盒 红色荧光.pdf
