Screen Quest 活细胞cAMP检测（含细胞系）
 |
货号 |
36384 |
存储条件 |
在零下15度以下保存, 避免光照 |
| 规格 |
1000 Tests |
价格 |
60588 |
| Ex (nm) |
490 |
Em (nm) |
520 |
| 分子量 |
|
溶剂 |
|
| 产品详细介绍 |
简要概述
产品基本信息
货号：36384
产品名称：Screen Quest cAMP活细胞检测（含细胞系）
规格：1000 Tests
储存条件：-15℃避光防潮
保质期：24个月
产品物理化学光谱特性
激发波长(nm)：490
发射波长(nm)：520
产品介绍
钙通量（通过Gqpathway偶联）测定法是药物发现中筛选GPCR靶标的首选方法。但是，超过60％的已知GPCR通过与cAMP偶联的腺苷酸环化酶活性发出信号。大多数现有的cAMP分析不仅需要细胞裂解，而且缺乏时间和空间分辨率。 Screen Quest 活细胞cAMP检测试剂盒以高通量形式对细胞内cAMP的变化进行实时检测，而无需细胞裂解步骤。该检测法通过包含外源性环状核苷酸门控通道（CNGC）或混杂G蛋白Gα16的细胞系进行。该通道被升高的细胞内cAMP水平激活，导致离子通量和细胞膜去极化，可以用荧光钙（例如Calbryte 520 AM，Cal-520 AM，Fluo-8 AM或Fluo-4 AM和相应的钙离子，免洗洗钙试剂盒）或荧光膜电位染料进行钙信号的检测。 Gα16与特定的非Gq偶联受体的共表达将在受体刺激后导致细胞内钙信号的产生。 Screen Quest 活细胞cAMP检测试剂盒提供了细胞系和试剂，可通过FLIPR，FDSS或其他等效的荧光酶标仪来检测细胞内cAMP的变化，它已成功用于检测Gs和Gi偶联的GPCR活性。
图示
图1. Screen Quest 活细胞cAMP检测原理
参考文献
A cardiac mitochondrial cAMP signaling pathway regulates calcium accumulation, permeability transition and cell death
Authors: Wang Z, Liu D, Varin A, Nicolas V, Courilleau D, Mateo P, Caubere C, Rouet P, Gomez AM, V and ecasteele G, Fischmeister R, Brenner C.
Journal: Cell Death Dis (2016): e2198
Activation of P2X7 and P2Y11 purinergic receptors inhibits migration and normalizes tumor-derived endothelial cells via cAMP signaling
Authors: Avanzato, D and Genova, T and Pla, A Fiorio and Bernardini, M and Bianco, S and Bussolati, B and Mancardi, D and Giraudo, E and Maione, F and Cassoni, P and others
Journal: Scientific Reports (2016)
Changes in the Arabidopsis thaliana Proteome Implicate cAMP in Biotic and Abiotic Stress Responses and Changes in Energy Metabolism
Authors: Alqurashi M, Gehring C, Marondedze C.
Journal: Int J Mol Sci (2016): 852
Odor-induced cAMP production in Drosophila melanogaster olfactory sensory neurons
Authors: Miazzi F, Hansson BS, Wicher D.
Journal: J Exp Biol (2016): 1798
Role of the cAMP Pathway in Glucose and Lipid Metabolism
Authors: Ravnskjaer K, Madiraju A, Montminy M.
Journal: Handb Exp Pharmacol (2016): 29
The pleiotropic role of exchange protein directly activated by cAMP 1 (EPAC1) in cancer: implications for therapeutic intervention
Authors: Almahariq M, Mei FC, Cheng X.
Journal: Acta Biochim Biophys Sin (Shanghai) (2016): 75
cAMP-Induced Histones H3 Dephosphorylation Is Independent of PKA and MAP Kinase Activations and Correlates With mTOR Inactivation
Authors: Rodriguez P, Rojas J.
Journal: J Cell Biochem (2016): 741
A cAMP Biosensor-Based High-Throughput Screening Assay for Identification of Gs-Coupled GPCR Ligands and Phosphodiesterase Inhibitors
Authors: Vedel L, Brauner-Osborne H, Mathiesen JM.
Journal: J Biomol Screen (2015): 849
Cardiac Hypertrophy Is Inhibited by a Local Pool of cAMP Regulated by Phosphodiesterase 2
Authors: Zoccarato A, Surdo NC, Aronsen JM, Fields LA, Mancuso L, Dodoni G, Stangherlin A, Livie C, Jiang H, Sin YY, Gesellchen F, Terrin A, Baillie GS, Nicklin SA, Graham D, Szabo-Fresnais N, Krall J, V and eput F, Movsesian M, Furlan L, Corsetti V, Hamilton G, Lefkimmiatis K, Sjaastad I, Zaccolo M.
Journal: Circ Res (2015): 707
Cardiac cAMP: production, hydrolysis, modulation and detection
Authors: Boularan C, Gales C.
Journal: Front Pharmacol (2015): 203
说明书
Screen Quest 活细胞cAMP检测（含细胞系）.pdf
