AA-dUTP [氨基烯丙基 dUTP 钠盐] *4 mM Tris 缓冲液 (pH 7.5)* *CAS 936327-10-5* 货号17005-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。

AA-dUTP [氨基烯丙基 dUTP 钠盐] *4 mM Tris 缓冲液 (pH 7.5)* *CAS 936327-10-5*

AA-dUTP [氨基烯丙基 dUTP 钠盐] *4 mM Tris 缓冲液 (pH 7.5)* *CAS 936327-10-5*

AA-dUTP [氨基烯丙基 dUTP 钠盐] *4 mM Tris 缓冲液 (pH 7.5)* *CAS 936327-10-5*    货号17005 货号 17005 存储条件 在零下15度以下保存, 避免光照
规格 2.5 umoles 价格 2604
Ex (nm) Em (nm)
分子量 589.17 溶剂 Water
产品详细介绍

简要概述

产品基本信息

货号:17005

产品名称:AA-dUTP [氨基烯丙基 dUTP 钠盐] *4 mM Tris 缓冲液 (pH 7.5)* *CAS 936327-10-5*

CAS:936327-10-5

规格:2.5umoles

储存条件:-15℃避光防潮

保质期:12个月

 

产品物理化学光谱特性

分子量:589.17

溶剂:水

激发波长(nm):N/A

发射波长(nm):N/A

 

产品介绍

氨基烯丙基-DUTP钠盐 4mM AA-dUTP是美国AAT Bioquest生产的荧光染料,胺修饰的脱氧尿苷5′-三磷酸酯(如氨基烯丙基-dUTP)可用于通过常规酶掺入方法(如反转录,缺口翻译,随机引物标记或PCR)生产含胺的DNA。氨基烯丙基dUTP可以通过常规酶结合技术轻松地掺入DNA中。然后可以使用任何胺反应性荧光染料,生物素和其他胺反应性试剂对所得的胺修饰的核酸进行标记。与通常具有较高立体抑制能力的标签标记的尿苷三磷酸酯相比,氨基烯丙基修饰的核苷酸可以掺入到极高且一致的水平。胺修饰的核酸与过量的胺反应性试剂的随后反应,无论选择何种标记试剂,均能实现相应较高的一致标记效率。这种两步标记方法还消除了优化酶促反应以适应不同染料修饰核苷酸的需要,该修饰可能以非常不同的速率掺入。这种标记方法广泛用于FISH探针和基于微阵列的实验。金畔生物是AAT Bioquest的中国代理商,为您提供最优质的氨基烯丙基-DUTP钠盐 4mM AA-dUTP 。

 

参考文献

A visual DNA chip for simultaneous detection of hepatitis B virus, hepatitis C virus and human immunodeficiency virus type-1
Authors: Wen JK, Zhang XE, Cheng Z, Liu H, Zhou YF, Zhang ZP, Yang JH, Deng JY.
Journal: Biosens Bioelectron (2004): 685

Fluorescent DNA hybridization probe preparation using amine modification and reactive dye coupling
Authors: Cox WG, Singer VL.
Journal: Biotechniques (2004): 114

Aminomodified nucleobases: functionalized nucleoside triphosphates applicable for SELEX
Authors: Schoetzau T, Langner J, Moyroud E, Roehl I, Vonhoff S, Klussmann S.
Journal: Bioconjug Chem (2003): 919

Simple method for preparation of fluor/hapten-labeled dUTP
Authors: Nimmakayalu M, Henegariu O, Ward DC, Bray-Ward P.
Journal: Biotechniques (2000): 518

Topology of yeast RNA polymerase II subunits in transcription elongation complexes studied by photoaffinity cross-linking
Authors: Wooddell CI, Burgess RR.
Journal: Biochemistry (2000): 13405

Quantitative analysis of polymerase chain reaction products using biotinylated dUTP incorporation
Authors: Duplaa C, Couffinhal T, Labat L, Moreau C, Lamaziere JM, Bonnet J.
Journal: Anal Biochem (1993): 229

A non-radioisotopic reverse transcriptase assay using biotin-11-deoxyuridinetriphosphate on primer-immobilized microtiter plates
Authors: Urabe T, Sano K, Tanno M, Mizoguchi J, Otani M, Lee MH, Takasaki T, Kusakabe H, Imagawa DT, Nakai M.
Journal: J Virol Methods (1992): 145

Affinity isolation of active murine erythroleukemia cell chromatin: uniform distribution of ubiquitinated histone H2A between active and inactive fractions
Authors: Dawson BA, Herman T, Haas AL, Lough J.
Journal: J Cell Biochem (1991): 166

Non-radioactive labeling and detection of nucleic acids. IV. Synthesis and properties of digoxigenin-modified 2′-deoxyuridine-5′-triphosphates and a photoactivatable analog of digoxigenin (photodigoxigenin)
Authors: Muhlegger K, Huber E, von der Eltz H, Ruger R, Kessler C.
Journal: Biol Chem Hoppe Seyler (1990): 953

说明书
AA-dUTP [氨基烯丙基 dUTP 钠盐] *4 mM Tris 缓冲液 (pH 7.5)* *CAS 936327-10-5*.pdf

AF 488 NHS酯 等同于Alexa Fluor 488 货号1812-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。

AF 488 NHS酯 等同于Alexa Fluor 488

AF 488 NHS酯 等同于Alexa Fluor 488

货号 1812 存储条件 在零下15度以下保存, 避免光照
规格 1 mg 价格 2604
Ex (nm) 499 Em (nm) 520
分子量 643.40 溶剂 DMSO
产品详细介绍

简要概述

产品基本信息

货号:1812

产品名称:Alpha Fluor 488 NHS酯

规格:1mg

储存条件:保存在冰箱-15℃干燥

保质期:12个月

 

产品物理化学光谱特性

分子量:643.40

外观:固体

溶剂:DMSO

激发波长(nm):499

发射波长(nm):520

 

产品介绍

Alpha Fluor 488 NHS酯(琥珀酰亚胺酯)与AlexaFluor®488NHS酯相同(AlexaFluor®是ThermoFisher的商标)。它是一种明亮的绿色荧光染料,最适合与488 nm氩激光器配合使用。Alpha Fluor 488染料在pH 4至pH 10下是水溶性和pH不敏感的。Alpha Fluor 488的NHS酯(或琥珀酰亚胺基酯)是最方便的胺反应形式,用于将该染料与蛋白质或抗体结合。

点击查看光谱

点击查看实验方案

 

参考文献

Development of new hCaM-Alexa Fluor(R) biosensors for a wide range of ligands
Authors: Velazquez-Lopez, I.; Leon-Cruz, E.; Pardo, J. P.; Sosa-Peinado, A.; Gonzalez-Andrade, M.
Journal: Anal Biochem (2017): 13-22

Synthetic Protocol for AFCS: A Biologically Active Fluorescent Castasterone Analog Conjugated to an Alexa Fluor 647 Dye
Authors: Winne, J. M.; Irani, N. G.; Van den Begin, J.; Madder, A.
Journal: Methods Mol Biol (2017): 21-Sep

Alteration of AMPA Receptor-Mediated Synaptic Transmission by Alexa Fluor 488 and 594 in Cerebellar Stellate Cells
Authors: Maroteaux, M.; Liu, S. J.
Journal: eNeuro (2016)

Alexa fluor-labeled fluorescent cellulose nanocrystals for bioimaging solid cellulose in spatially structured microenvironments
Authors: Grate, J. W.; Mo, K. F.; Shin, Y.; Vasdekis, A.; Warner, M. G.; Kelly, R. T.; Orr, G.; Hu, D.; Dehoff, K. J.; Brockman, F. J.; Wilkins, M. J.
Journal: Bioconjug Chem (2015): 593-601

A mouse monoclonal antibody against Alexa Fluor 647
Authors: Wuethrich, I.; Guillen, E.; Ploegh, H. L.
Journal: Monoclon Antib Immunodiagn Immunother (2014): 109-20

In vivo visualization of GL261-luc2 mouse glioma cells by use of Alexa Fluor-labeled TRP-2 antibodies
Authors: Fenton, K. E.; Martirosyan, N. L.; Abdelwahab, M. G.; Coons, S. W.; Preul, M. C.; Scheck, A. C.
Journal: Neurosurg Focus (2014): E12

Green tea catechins quench the fluorescence of bacteria-conjugated Alexa fluor dyes
Authors: Zhao, L.; Li, W.; Zhu, S.; Tsai, S.; Li, J.; Tracey, K. J.; Wang, P.; Fan, S.; Sama, A. E.; Wang, H.
Journal: Inflamm Allergy Drug Targets (2013): 308-14

Volume labeling with Alexa Fluor dyes and surface functionalization of highly sensitive fluorescent silica (SiO2) nanoparticles
Authors: Wang, W.; Nallathamby, P. D.; Foster, C. M.; Morrell-Falvey, J. L.; Mortensen, N. P.; Doktycz, M. J.; Gu, B.; Retterer, S. T.
Journal: Nanoscale (2013): 10369-75

Fluorescence of Alexa fluor dye tracks protein folding
Authors: Lindhoud, S.; Westphal, A. H.; Visser, A. J.; Borst, J. W.; van Mierlo, C. P.
Journal: PLoS One (2012): e46838

a Luo-acupoint) in the rat: a double-labeling study of cholera toxin subunit B conjugated with Alexa Fluor 488 and 594]
Authors: Cui, J. J.; Zhu, X. L.; Ji, C. F.; Jing, X. H.; Bai, W. Z., [Neuroanatomical basis of clinical joint application of “Jinggu” (BL 64, a source-acupoint) and “Dazhong” (KI 4
Journal: Zhen Ci Yan Jiu (2011): 262-7

 

相关产品

产品名称 货号
iFluor 488琥珀酰亚胺酯 Cat#1023
AF 594 NHS酯 等同于Alexa Fluor 594 Cat#1830
AF 350 NHS酯 等同于Alexa Fluor 350 Cat#1800

说明书
AF 488 NHS酯 等同于Alexa Fluor 488.pdf

链霉亲和素偶联物 Texas Red-标记 货号16911-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。

链霉亲和素偶联物 Texas Red-标记

链霉亲和素偶联物 Texas Red-标记

链霉亲和素偶联物 Texas Red-标记    货号16911 货号 16911 存储条件 在零下15度以下保存, 避免光照
规格 1 mg 价格 1944
Ex (nm) 586 Em (nm) 603
分子量 ~52000 溶剂 Water
产品详细介绍

简要概述

产品基本信息

货号:16911

产品名称:链霉亲和素偶联物 Texas Red-标记

规格:100ug

储存条件:-15℃避光防潮

保质期:12个月

 

产品物理化学光谱特性

分子量:N/A

溶剂:水

激发波长(nm):585

发射波长(nm):602

 

产品介绍

链霉亲和素偶联物 Texas Red-标记是美国AAT Bioquest生产的链霉亲和素,链霉亲和素缀合物与生物素缀合物一起广泛用于特异性检测多种蛋白质,蛋白质基序,核酸和其他分子,因为链霉亲和素对生物素具有非常高的结合亲和力。该德克萨斯红-链霉亲和素缀合物包含链霉亲和素(作为生物素结合蛋白),共价连接了德克萨斯红(作为荧光标记)。我们的德克萨斯州红色链霉亲和素偶联物是使用AAT Bioquest的专有标记技术制备的。与来自其他商业来源的类似的德克萨斯红-链霉亲和素缀合物相比,它显示出了更亮的信号,因此可以显着提高测定的灵敏度。当与生物素化的一抗结合使用时,通常用作间接免疫荧光染色的第二步试剂。它是使用流式细胞仪和德克萨斯红滤光片组进行基于生物素-链霉亲和素的生物学测定和测试的宝贵工具。多种互补的生物素化试剂可从许多商业供应商处获得。金畔生物是AAT Bioquest的中国代理商,为您提供最优质的链霉亲和素偶联物 Texas Red-标记。 

点击查看光谱

点击查看iFluor系列产品光谱

 

参考文献

Overexpression of MACC1 and the association with hepatocyte growth factor/c-Met in epithelial ovarian cancer
Authors: Hongyu Li, Hui Zhang, Shujun Zhao, Yun Shi, Junge Yao, Yanyan Zhang, Huanhuan Guo, Xingsuo Liu
Journal: Oncology letters (2015): 1989–1996

 

相关产品

产品名称 货号
链霉亲和素 HRP-标记 Cat#16920
链霉亲和素偶联物 iFluor 647-标记 Cat#16966
链霉亲和素偶联物 trFluor Eu-标记 Cat#16925

说明书
链霉亲和素偶联物 Texas Red-标记.pdf

AA-dUTP [氨基烯丙基 dUTP 钠盐] *4 mM Tris 缓冲液 (pH 7.5)* *CAS 936327-10-5* 货号17004-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。

AA-dUTP [氨基烯丙基 dUTP 钠盐] *4 mM Tris 缓冲液 (pH 7.5)* *CAS 936327-10-5*

AA-dUTP [氨基烯丙基 dUTP 钠盐] *4 mM Tris 缓冲液 (pH 7.5)* *CAS 936327-10-5*

AA-dUTP [氨基烯丙基 dUTP 钠盐] *4 mM Tris 缓冲液 (pH 7.5)* *CAS 936327-10-5*    货号17004 货号 17004 存储条件 在零下15度以下保存, 避免光照
规格 1 umole 价格 1272
Ex (nm) Em (nm)
分子量 589.17 溶剂 Water
产品详细介绍

简要概述

产品基本信息

货号:17004

产品名称:AA-dUTP [氨基烯丙基 dUTP 钠盐] *4 mM Tris 缓冲液 (pH 7.5)* *CAS 936327-10-5*

CAS:936327-10-5

规格:1 umole

储存条件:-15℃避光防潮

保质期:12个月

 

产品物理化学光谱特性

分子量:589.17

溶剂:水

激发波长(nm):N/A

发射波长(nm):N/A

 

产品介绍

氨基烯丙基-DUTP钠盐 4mM AA-dUTP是美国AAT Bioquest生产的荧光染料,胺修饰的脱氧尿苷5′-三磷酸酯(如氨基烯丙基-dUTP)可用于通过常规酶掺入方法(如反转录,缺口翻译,随机引物标记或PCR)生产含胺的DNA。氨基烯丙基dUTP可以通过常规酶结合技术轻松地掺入DNA中。然后可以使用任何胺反应性荧光染料,生物素和其他胺反应性试剂对所得的胺修饰的核酸进行标记。与通常具有较高立体抑制能力的标签标记的尿苷三磷酸酯相比,氨基烯丙基修饰的核苷酸可以掺入到极高且一致的水平。胺修饰的核酸与过量的胺反应性试剂的随后反应,无论选择何种标记试剂,均能实现相应较高的一致标记效率。这种两步标记方法还消除了优化酶促反应以适应不同染料修饰核苷酸的需要,该修饰可能以非常不同的速率掺入。这种标记方法广泛用于FISH探针和基于微阵列的实验。金畔生物是AAT Bioquest的中国代理商,为您提供最优质的氨基烯丙基-DUTP钠盐 4mM AA-dUTP 。

 

参考文献

A visual DNA chip for simultaneous detection of hepatitis B virus, hepatitis C virus and human immunodeficiency virus type-1
Authors: Wen JK, Zhang XE, Cheng Z, Liu H, Zhou YF, Zhang ZP, Yang JH, Deng JY.
Journal: Biosens Bioelectron (2004): 685

Fluorescent DNA hybridization probe preparation using amine modification and reactive dye coupling
Authors: Cox WG, Singer VL.
Journal: Biotechniques (2004): 114

Aminomodified nucleobases: functionalized nucleoside triphosphates applicable for SELEX
Authors: Schoetzau T, Langner J, Moyroud E, Roehl I, Vonhoff S, Klussmann S.
Journal: Bioconjug Chem (2003): 919

Simple method for preparation of fluor/hapten-labeled dUTP
Authors: Nimmakayalu M, Henegariu O, Ward DC, Bray-Ward P.
Journal: Biotechniques (2000): 518

Topology of yeast RNA polymerase II subunits in transcription elongation complexes studied by photoaffinity cross-linking
Authors: Wooddell CI, Burgess RR.
Journal: Biochemistry (2000): 13405

Quantitative analysis of polymerase chain reaction products using biotinylated dUTP incorporation
Authors: Duplaa C, Couffinhal T, Labat L, Moreau C, Lamaziere JM, Bonnet J.
Journal: Anal Biochem (1993): 229

A non-radioisotopic reverse transcriptase assay using biotin-11-deoxyuridinetriphosphate on primer-immobilized microtiter plates
Authors: Urabe T, Sano K, Tanno M, Mizoguchi J, Otani M, Lee MH, Takasaki T, Kusakabe H, Imagawa DT, Nakai M.
Journal: J Virol Methods (1992): 145

Affinity isolation of active murine erythroleukemia cell chromatin: uniform distribution of ubiquitinated histone H2A between active and inactive fractions
Authors: Dawson BA, Herman T, Haas AL, Lough J.
Journal: J Cell Biochem (1991): 166

Non-radioactive labeling and detection of nucleic acids. IV. Synthesis and properties of digoxigenin-modified 2′-deoxyuridine-5′-triphosphates and a photoactivatable analog of digoxigenin (photodigoxigenin)
Authors: Muhlegger K, Huber E, von der Eltz H, Ruger R, Kessler C.
Journal: Biol Chem Hoppe Seyler (1990): 953

说明书
AA-dUTP [氨基烯丙基 dUTP 钠盐] *4 mM Tris 缓冲液 (pH 7.5)* *CAS 936327-10-5*.pdf

AMCA C2马来酰亚胺 货号503-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。

AMCA C2马来酰亚胺

AMCA C2马来酰亚胺

AMCA C2马来酰亚胺    货号503 货号 503 存储条件 在零下15度以下保存, 避免光照
规格 5 mg 价格 2604
Ex (nm) 346 Em (nm) 434
分子量 355.34 溶剂 DMSO
产品详细介绍

简要概述

产品基本信息

货号:503

产品名称:AMCA C2马来酰亚胺

规格:5mg

储存条件:-15℃避光防潮

保质期:12个月

 

产品物理化学光谱特性

分子量:355.34

溶剂:DMSO

激发波长(nm):334

发射波长(nm):342

 

产品介绍

AMCA C2马来酰亚胺是美国AAT Bioquest生产的荧光染料。AMCA 是一种强烈的蓝色荧光染料,在365nm处被激发。它具有很强的耐光性,而且在pH4~10之间都能产生荧光。金畔生物是AAT Bioquest的中国代理商,为您提供最优质的AMCA C2马来酰亚胺。 

点击查看光谱

 

参考文献

Functional expression of the peptide transporter PEPT2 in the mammalian enteric nervous system
Authors: Ruhl A, Hoppe S, Frey I, Daniel H, Schemann M.
Journal: J Comp Neurol (2005): 1

Increased resonance energy transfer between fluorophores bound to DNA in proximity to metallic silver particles
Authors: Malicka J, Gryczynski I, Fang J, Kusba J, Lakowicz JR.
Journal: Anal Biochem (2003): 160

Dental extractions in patients maintained on oral anticoagulant therapy: comparison of INR value with occurrence of postoperative bleeding
Authors: Blinder D, Manor Y, Martinowitz U, Taicher S.
Journal: Int J Oral Maxillofac Surg (2001): 518

[Hemostatic wound management in marcumar patients. Collagen fleece vs. tranexamic acid]
Authors: Bublitz R, Sommer S, Weingart D, Bauerle K, Both A.
Journal: Mund Kiefer Gesichtschir (2000): 240

[Oral surgical procedures during anticoagulant therapy]
Authors: Blinder D, Martinowitz U, Ardekian L, Peleg M, Taicher S.
Journal: Harefuah (1996): 681

Evidence for cobinding of self- and allopeptides to human class II major histocompatibility antigen DR1 by energy transfer
Authors: Kropshofer H, Max H, Kalbacher H.
Journal: Proc Natl Acad Sci U S A (1993): 403

Soluble aggregates of IgG and immune complexes enhance IL-6 production by renal mesangial cells
Authors: van den Dobbelsteen ME, van der Woude FJ, Schroeijers WE, van Es LA, Daha MR.
Journal: Kidney Int (1993): 544

Time-resolved energy transfer measurements of donor-acceptor distance distributions and intramolecular flexibility of a CCHH zinc finger peptide
Authors: Eis PS, Lakowicz JR.
Journal: Biochemistry (1993): 7981

Improved indirect fluorescence immunocytochemical method using counterstains
Authors: Newkirk RF, Mack J.
Journal: Biotechniques (1992): 536

Triple immunofluorescence confocal laser scanning microscopy: spatial correlation of novel cellular differentiation markers in human muscle biopsies
Authors: Schubert W.
Journal: Eur J Cell Biol (1991): 272

说明书
AMCA C2马来酰亚胺.pdf