标签归档：peg

AF532 PEG4 生物素偶联物 货号3110-AAT Bioquest荧光染料

发表于由

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品，欢迎访问AAT Bioquest荧光染料官网了解更多信息。

AF532 PEG4 生物素偶联物

AF532 PEG4 生物素偶联物

AF532 PEG4 生物素偶联物 货号3110 货号 3110 存储条件 在零下15度以下保存, 避免光照
规格 1 mg 价格 2340
Ex (nm) 534 Em (nm) 553
分子量 1172.48 溶剂 Water
产品详细介绍

简要概述

产品基本信息

货号：3110

产品名称：AF532 PEG4 生物素偶联物

规格：1mg

储存条件：保存在冰箱-15℃干燥

保质期：12个月

 

产品物理化学光谱特性

分子量：1172.48

外观：固体

溶剂：水

激发波长(nm)：534

发射波长(nm)：553

 

产品介绍

AF532 PEG4 生物素偶联物含有 Alexa Fluor 532 荧光团，可被常见的 532 nm 激光很好地激发。它在亲和素和链霉亲和素四聚体的高亲和力、快速结合和非协同结合方面具有与天然生物素相似的亲和素结合特性。 AF532 PEG4 生物素偶联物可用于检测和量化抗生物素蛋白、链霉抗生物素蛋白或中性抗生物素蛋白的生物素结合位点。该试剂克服了常用的 Biotin-4-fluorescein 的主要缺点，例如在水性介质中的溶解度差和 pH 依赖性吸光度和短波长发射。 AF532 PEG4 生物素偶联物是一种水溶性试剂，具有从 pH 4 到 pH 10 的不依赖 pH 的荧光。生物素部分和荧光标签之间的柔性 PEG4 间隔基可最大限度地减少与抗生物素蛋白、链霉抗生物素蛋白或中性抗生物素蛋白结合所涉及的空间位阻。

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参考文献

An In Vitro Assay to Monitor Sertoli Cell Blood-Testis Barrier (BTB) Integrity.
Authors: Wu, Siwen and Wang, Lingling and Tang, Elizabeth I and Wang, Junlu and Cheng, C Yan
Journal: Methods in molecular biology (Clifton, N.J.) (2021)
Effective Tumor Targeting by EphA2-Agonist-Biotin-Streptavidin Conjugates.
Authors: Udompholkul, Parima and Baggio, Carlo and Gambini, Luca and Sun, Yu and Zhao, Ming and Hoffman, Robert M and Pellecchia, Maurizio
Journal: Molecules (Basel, Switzerland) (2021)
Highly sensitive detection of DNA methyltransferase activity and its inhibitor screening by coupling fluorescence correlation sp
Authors: Huang, Yuyang and Deng, Liyun and Su, Di and Huang, Xiangyi and Ren, Jicun
Journal: The Analyst (2021)
Fluorescence In Situ Hybridization of Small Non-Coding RNAs.
Authors: Vautrot, Valentin and Heckler, Géraud and Aigueperse, Christelle and Behm-Ansmant, Isabelle
Journal: Methods in molecular biology (Clifton, N.J.) (2021): 73-85
Effect of amisulpride, olanzapine, quetiapine, and aripiprazole single administration on c-Fos expression in vasopressinergic an
Authors: Kiss, Alexander and Osacka, Jana
Journal: Endocrine regulations (2020): 77-84
Integration of Enzymatic Labeling with Single-Molecule Detection for Sensitive Quantification of Diverse DNA Damages.
Authors: Zhang, Yan and Hua, Ruo-Nan and Zhang, Chun-Yang
Journal: Analytical chemistry (2020): 4700-4706
Enhanced broadband fluorescence detection of nucleic acids using multipolar gap-plasmons on biomimetic Au metasurfaces.
Authors: Narasimhan, Vinayak and Siddique, Radwanul Hasan and Hoffmann, Magnus and Kumar, Shailabh and Choo, Hyuck
Journal: Nanoscale (2019): 13750-13757
Egg autofluorescence and options for detecting peanut agglutinin binding for the identification of Haemonchus contortus eggs in
Authors: Abbas, Ibrahim and Hildreth, Michael
Journal: Veterinary parasitology (2019): 69-74
A Vector-Based Method to Analyze the Topography of Glial Networks.
Authors: Eitelmann, Sara and Hirtz, Jan J and Stephan, Jonathan
Journal: International journal of molecular sciences (2019)
Photobleaching Comparison of R-Phycoerythrin-Streptavidin and Streptavidin-Alexa Fluor 568 in a Breast Cancer Cell Line.
Authors: Ostad, Seyed Nasser and Babaei, Sepideh and Bayat, Ali Ahmad and Mahmoudian, Jafar
Journal: Monoclonal antibodies in immunodiagnosis and immunotherapy (2019): 25-29

说明书
AF532 PEG4 生物素偶联物.pdf

MM（PEG）24甲基-PEG-马来酰亚胺试剂 CAS 88504-24-9 货号4525-AAT Bioquest荧光染料

发表于由

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品，欢迎访问AAT Bioquest荧光染料官网了解更多信息。

MM（PEG）24甲基-PEG-马来酰亚胺试剂 CAS 88504-24-9

MM（PEG）24甲基-PEG-马来酰亚胺试剂 CAS 88504-24-9

MM（PEG）24甲基-PEG-马来酰亚胺试剂 CAS 88504-24-9 货号4525 货号 4525 存储条件 在零下15度以下保存
规格 25 mg 价格 1272
Ex (nm) Em (nm)
分子量 1239.45 溶剂 Water
产品详细介绍

简要概述

产品基本信息

货号：4525

产品名称：MM（PEG）24甲基-PEG-马来酰亚胺试剂

CAS：88504-24-9

规格：25mg

储存条件：-15℃避光防潮

保质期：12个月

 

产品物理化学光谱特性

分子量：1239.45

外观：固体

溶剂：水

激发波长（nm）：N/A

发射波长（nm）：N/A

 

产品介绍

MM（PEG）24甲基-PEG-马来酰亚胺试剂是美国AAT Bioquest生产的荧光染料。MM（PEG）24是用马来酰亚胺基团活化的甲基封端的聚乙二醇化合物（24个PEG单元），用于在生物分子或测定表面上共价聚乙二醇化巯基。MM（PEG）24用于在pH 6.5-7.5下有效PEG化巯基。它具有完整的PEG链长度特征和定义，具有不连续的分子量，可在蛋白质修饰应用中保持一致的性能。其PEG间隔物提供独特的优势，包括增加的稳定性，降低的聚集倾向和降低的免疫原性。该PEG试剂已用于PEG化测定表面，向蛋白质，免疫原，药物化合物和探针添加惰性物质，改善蛋白质或肽的溶解度（降低聚集）而不影响功能，并保护蛋白质免于蛋白水解。含PEG试剂已用于修饰蛋白质以提供特定的优点。蛋白质PEG化可以提高修饰蛋白质的稳定性，保护其免受蛋白水解消化，增加其在生物应用中的半衰期，掩盖其免于引起免疫原性反应，降低其抗原性或潜在毒性，提高其溶解度，减少聚集的可能性，并最大限度地减少体外和体内应用的干扰。金畔生物是AAT Bioquest的中国代理商，为您提供最优质的MM（PEG）24甲基-PEG-马来酰亚胺试剂。 

 

参考文献

Loss of PEG chain in routine SDS-PAGE analysis of PEG-maleimide modified protein
Authors: C. Zhang
Journal: Electrophoresis (2015): 371-4

Protease-degradable PEG-maleimide coating with on-demand release of IL-1Ra to improve tissue response to neural electrodes
Authors: S. M. Gutowski
Journal: Biomaterials (2015): 55-70

Quantification of PEG-maleimide ligands and coupling efficiencies on nanoparticles with Ellman’s reagent
Authors: M. Moser
Journal: Anal Chem (2015): 9376-83

PEG-maleimide hydrogels for protein and cell delivery in regenerative medicine
Authors: A. J. Garcia
Journal: Ann Biomed Eng (2014): 312-22

Rapidly in situ forming adhesive hydrogel based on a PEG-maleimide modified polypeptide through Michael addition
Authors: Y. Zhou
Journal: J Mater Sci Mater Med (2013): 2277-86

N-glycan targeted gene delivery to the dendritic cell SIGN receptor
Authors: Kevin Anderson, Christian Fernandez, Kevin G Rice
Journal: Bioconjugate chemistry (2010): 1479–1485

说明书
MM（PEG）24甲基-PEG-马来酰亚胺试剂 CAS 88504-24-9.pdf

AzoDye-1-PEG10-叠氮化物 货号2413-AAT Bioquest荧光染料

发表于由

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品，欢迎访问AAT Bioquest荧光染料官网了解更多信息。

AzoDye-1-PEG10-叠氮化物

AzoDye-1-PEG10-叠氮化物

AzoDye-1-PEG10-叠氮化物 货号2413 货号 2413 存储条件 在零下15度以下保存, 避免光照
规格 1 mg 价格 1776
Ex (nm) Em (nm)
分子量 1013.16 溶剂 DMSO
产品详细介绍

简要概述

产品基本信息

货号：2413

产品名称：AzoDye-1-PEG10-叠氮化物

规格：1mg

储存条件：保存在冰箱-15℃干燥

保质期：12个月

 

产品物理化学光谱特性

分子量：1013.16

吸收：522

溶剂：DMSO


产品介绍

AzoDye-1 PEG10 叠氮化物是标记含炔分子的绝佳构建模块。 它包含一个 PEG10 间隔基，与 AzoDye-1 叠氮化物 (#2412) 相比，提高了水溶性，尽管 AzoDye-1 叠氮化物 (#2412) 和 AzoDye-1 PEG10 叠氮化物 (#2413) 都具有相同的疏水的 BHQ-1 生色团 . AzoDye-1 染料几乎不发荧光，具有中等消光系数。 这些暗猝灭剂最常用于分子信标探针，其中 FRET 对其荧光猝灭的贡献最小，因此对供体-受体光谱重叠的依赖性对于其他 FRET 探针（如 FRET 肽）并不重要。 AzoDye-1 染料可以淬灭多种荧光团，特别是荧光素衍生物，如 FAM、HEX、TET、NED 和 JOE。金畔生物是AAT Bioquest的中国代理商，为您提供最优质的AzoDye-1-PEG10-叠氮化物。 

点击查看光谱

 

参考文献

A novel method for visualizing and tracking endogenous mRNA in a specific cell population in pathological neovascularization.
Authors: Uddin, Md Imam and Kilburn, Tyler C and Jamal, Sara Z and Duvall, Craig L and Penn, John S
Journal: Scientific reports (2021): 2565

A phosphorescence resonance energy transfer-based “off-on” long afterglow aptasensor for cadmium detection in food samples.
Authors: Lai, Bin and Wang, Haitao and Su, Wentao and Wang, Zhouping and Zhu, Bei-Wei and Yu, Chenxu and Tan, Mingqian
Journal: Talanta (2021): 122409

DNA functionalized double quantum dots-based fluorescence biosensor for one-step simultaneous detection of multiple microRNAs.
Authors: Hu, Ou and Li, Zeyu and Tong, Yanli and Wang, Qiyou and Chen, Zuanguang
Journal: Talanta (2021): 122763

Detection of Amyloid β Oligomers by a Fluorescence Ratio Strategy Based on Optically Trapped Highly Doped Upconversion Nanoparticles-SiO2@Metal-Organic Framework Microspheres.
Authors: Fang, Wen-Kai and Liu, Liu and Zhang, Li-Ling and Liu, Da and Liu, Yang and Tang, Hong-Wu
Journal: Analytical chemistry (2021): 12447-12455

High-Sensitivity Sensing of Divalent Copper Ions at the Single Upconversion Nanoparticle Level.
Authors: Wang, Xindong and Zhang, Xiaorong and Huang, Dingxin and Zhao, Tianyu and Zhao, Lili and Fang, Xikui and Yang, Chunhui and Chen, Guanying
Journal: Analytical chemistry (2021): 11686-11691

Lighting up ATP in cells and tissues using a simple aptamer-based fluorescent probe.
Authors: Liu, Wenjun and Zhu, Xuena and Mozneb, Maedeh and Nagahara, Larry and Hu, Tony Y and Li, Chen-Zhong
Journal: Mikrochimica acta (2021): 352

Modification of a SERS-active Ag surface to promote adsorption of charged analytes: effect of Cu2+ ions.
Authors: Ranishenka, Bahdan V and Panarin, Andrei Yu and Chelnokova, Irina A and Terekhov, Sergei N and Mojzes, Peter and Shmanai, Vadim V
Journal: Beilstein journal of nanotechnology (2021): 902-912

Multifunctional Programmable DNA Nanotrain for Activatable Hypoxia Imaging and Mitochondrion-Targeted Enhanced Photodynamic Therapy.
Authors: Liu, Jin and Ding, Ge and Chen, Shiya and Xue, Caoye and Chen, Mian and Wu, Xu and Yuan, Quan and Zheng, Jing and Yang, Ronghua
Journal: ACS applied materials & interfaces (2021): 9681-9690

Near-infrared light excited UCNP-DNAzyme nanosensor for selective detection of Pb2+ and in vivo imaging.
Authors: Huang, Linna and Chen, Feng and Zong, Xia and Lu, Qiujun and Wu, Cuiyan and Ni, Ziqi and Liu, Meiling and Zhang, Youyu
Journal: Talanta (2021): 122156

A Dendron-Based Fluorescence Turn-On Probe for Tumor Detection.
Authors: Liu, Changren and Zhang, Ling’e and Zhou, Sensen and Zhang, Xiaoke and Wu, Wei and Jiang, Xiqun
Journal: Chemistry (Weinheim an der Bergstrasse, Germany) (2020): 13022-13030

说明书
AzoDye-1-PEG10-叠氮化物.pdf

DNP-PEG4 NHS酯 CAS 858126-78-0 货号2023-AAT Bioquest荧光染料

发表于由

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品，欢迎访问AAT Bioquest荧光染料官网了解更多信息。

DNP-PEG4 NHS酯 CAS 858126-78-0

DNP-PEG4 NHS酯 CAS 858126-78-0

DNP-PEG4 NHS酯 CAS 858126-78-0 货号2023 货号 2023 存储条件 在零下15度以下保存, 避免光照
规格 5 mg 价格 1164
Ex (nm) Em (nm)
分子量 528.47 溶剂 DMSO
产品详细介绍

简要概述

产品基本信息

货号：2023

产品名称：DNP-PEG4琥珀酰亚胺酯

CAS：858126-78-0

规格：5mg

储存条件：-15℃，避光干燥

保质期：12个月

 

物理化学光谱特性

分子量：528.47

溶剂：DMSO

 

产品介绍

与常用的DNP-X SE（＃2021）相比，DNP-PEG4酸具有更好的水溶性。它是可被抗DNP抗体识别的探针的胺反应性构建基块。它也可以用作与Trp或Tyr配对的出色的胺反应性FRET淬灭剂。它的末端NHS酯基很容易与伯胺反应形成稳定的酰胺键。亲水性PEG接头的增加使得与生物分子缀合的DNP基团的数目增加，而不会引起沉淀。

 

参考文献

Nano-Snowflower of Gold Nanoparticles-Ruthenium Metallopolymer-Carbon Nanotubes Binding Anti-DNP IgE Antibody.
Authors: Li, Huayang and Wu, Jie and Melnyczuk, John M and Olubi, Omotunde and Lewis, Lauchon I and Cao, Yang and Nagappan, Peri and Khan, Shafiq A and Ingram, Conrad W and Harruna, Issifu I
Journal: Journal of nanoscience and nanotechnology (2015): 5733-40

Peptide mimics of hapten DNP: the effect of affinity of anti-DNP monoclonal antibodies for the selection of phage-displayed mimotopes.
Authors: Kalaycioglu, Atila T and Russell, Peter H and Howard, Colin R
Journal: Journal of immunological methods (2011): 36-42

Generation of heterogeneous rabbit anti-DNP antibodies by gene conversion and hypermutation of rearranged VL and VH genes during clonal expansion of B cells in splenic germinal centers.
Authors: Sehgal, D and Schiaffella, E and Anderson, A O and Mage, R G
Journal: European journal of immunology (2000): 3634-44

Determination of the binding of ligands containing the N-2,4-dinitrophenyl group to bivalent monoclonal rat anti-DNP antibody using affinity capillary electrophoresis.
Authors: Mammen, M and Gomez, F A and Whitesides, G M
Journal: Analytical chemistry (1995): 3526-35

Fast solid support detection of human papillomavirus in in vitro amplified DNA using a DNP-anti DNP monoclonal antibody couple.
Authors: Baccard-Longère, M and Alpha-Bazin, B and Chypre, C and Sauvaigo, S and Téoule, R and Bernard, P and Seigneurin, J M
Journal: Journal of virological methods (1994): 29-38

[Effects of Celosia argentea and Cucurbita moschata extracts on anti-DNP IgE antibody production in mice].
Authors: Imaoka, K and Ushijima, H and Inouye, S and Takahashi, T and Kojima, Y
Journal: Arerugi = [Allergy] (1994): 652-9

[Effects of Perilla frutescens extract on anti-DNP IgE antibody production in mice].
Authors: Imaoka, K and Inouye, S and Takahashi, T and Kojima, Y
Journal: Arerugi = [Allergy] (1993): 74-80

Effect of anti-DNP IgG1- and IgG2a-secreting hybridomas in vivo on the development of an anti-DNP IgE antibody response in mice.
Authors: Hagen, M and Morrison, B and Robbinson, D and Strejan, G H
Journal: International archives of allergy and immunology (1992): 146-53

Mechanism of allergic cross-reactions–I. Multispecific binding of ligands to a mouse monoclonal anti-DNP IgE antibody.
Authors: Varga, J M and Kalchschmid, G and Klein, G F and Fritsch, P
Journal: Molecular immunology (1991): 641-54

Mechanism of allergic cross-reactions–II. Cross-stimulation, by chemically unrelated ligands, of rat basophilic leukemia cells sensitized with an anti-DNP IgE antibody.
Authors: Varga, J M and Kalchschmid, G and Klein, G F and Fritsch, P
Journal: Molecular immunology (1991): 655-9

说明书
DNP-PEG4 NHS酯 CAS 858126-78-0.pdf

鬼笔环肽-PEG4-DBCO 货号5303-AAT Bioquest荧光染料

发表于由

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品，欢迎访问AAT Bioquest荧光染料官网了解更多信息。

鬼笔环肽-PEG4-DBCO

鬼笔环肽-PEG4-DBCO

鬼笔环肽-PEG4-DBCO 货号5303 货号 5303 存储条件 在零下15度以下保存, 避免光照
规格 100 ug 价格 3732
Ex (nm) Em (nm)
分子量 1366.55 溶剂 DMSO
产品详细介绍

简要概述

产品基本信息

货号：5303

产品名称：鬼笔环肽-PEG4-DBCO

规格：100 ug

储存条件：-15℃避光防潮

保质期：24个月

 

产品物理化学光谱特性

分子量：1366.55

外观：固体

溶剂：DMSO

 

产品介绍

鬼笔环肽-PEG4-DBCO是美国AAT Bioquest生产的鬼笔环肽缀合物，鬼笔环肽Phalloidin标记探针会选择性地结合F-肌动蛋白。以纳摩尔浓度使用，鬼笔环肽衍生物是方便的探针，用于标记，鉴定和定量甲醛固定和透化组织切片中的F-肌动蛋白，细胞培养物或无细胞实验。肌动蛋白是一种球状的，大约42kDa的蛋白质，几乎存在于所有真核细胞中。 它也是最高度保守的蛋白质之一，与藻类和人类不同的物种相差不超过20％。 肌动蛋白是细胞中两种细丝的单体亚基：微丝，细胞骨架的三个主要组分之一，以及微丝，是肌细胞中收缩装置的一部分。 因此，肌动蛋白参与许多重要的细胞过程，包括肌肉收缩，细胞运动，细胞分裂和胞质分裂，囊泡和细胞器运动，细胞信号传导，以及细胞连接和细胞形状的建立和维持。

鬼笔环肽比肌动蛋白单体更紧密地结合肌动蛋白丝，导致肌动蛋白亚基从丝状末端解离的速率常数降低，基本上通过防止丝解聚来稳定肌动蛋白丝。此外，发现鬼笔环肽抑制F-肌动蛋白的ATP水解活性。鬼笔环肽在细胞中以不同浓度起作用不同。当以低浓度引入细胞质时，鬼笔环肽将较少聚合形式的细胞质肌动蛋白以及微丝蛋白聚集成聚集的肌动蛋白聚合物的稳定“岛”，但它不会干扰应力纤维，即厚的微丝束。鬼笔环肽的性质是通过用荧光类似物标记鬼笔环肽并用它们染色肌动蛋白丝用于光学显微镜来研究F-肌动蛋白在细胞中的分布的有用工具。鬼笔环肽的荧光衍生物已经证明在定位活细胞或固定细胞中的肌动蛋白丝以及在体外可视化单个肌动蛋白丝方面非常有用。荧光鬼笔环肽衍生物已被用作高分辨率肌动蛋白研究中的重要工具。 AAT Bioquest可以提供多色成像应用的鬼笔环肽。金畔生物是AAT Bioquest的中国代理商，为您提供最优质的鬼笔环肽-PEG4-DBCO。 

 

参考文献

Biomaterial Surface Can Modify HUVEC Morphology and Inflammatory Response by Regulating MicroRNA Expression
Authors: Gu, Shuangying and Tian, Baoxiang and Chen, Weicong and Zhou, Yue
Journal: Journal of Biosciences and Medicines (2017): 8

Cell-Permeable, MMP-2 Activatable, Nickel Ferrite and His-tagged Fusion Protein Self-Assembled Fluorescent Nanoprobe for Tumor Magnetic Targeting and Imaging
Authors: Sun, Lu and Xie, Shuping and Qi, Jing and Liu, Ergang and Liu, Di and Liu, Quan and Chen, Sunhui and He, Huining and Yang, Victor C
Journal: ACS Applied Materials & Interfaces (2017)

DNA Double-Strand Breaks Induce the Nuclear Actin Filaments Formation in Cumulus-Enclosed Oocytes but Not in Denuded Oocytes
Authors: Sun, Ming-Hong and Yang, Mo and Xie, Feng-Yun and Wang, Wei and Zhang, Lili and Shen, Wei and Yin, Shen and Ma, Jun-Yu
Journal: PloS one (2017): e0170308

Enhanced bovine serum albumin absorption on the N-hydroxysuccinimide activated graphene oxide and its corresponding cell affinity
Authors: Xiong, Kun and Fan, Qingbo and Wu, Tingting and Shi, Haishan and Chen, Lin and Yan, Minhao
Journal: Materials Science and Engineering: C (2017)

Enhanced osteointegration of tantalum-modified titanium implants with micro/nano-topography
Authors: Shi, Junyu and Zhang, Xiaomeng and Qiao, Shichong and Ni, Jie and Mo, Jiaji and Gu, Yingxin and Lai, Hongchang
Journal: RSC Advances (2017): 46472–46479

Microtopography Attenuates Endothelial Cell Proliferation by Regulating MicroRNAs
Authors: Wang, Dan and Liu, Mengya and Gu, Shuangying and Zhou, Yue and Li, Song
Journal: Journal of Biomaterials and Nanobiotechnology (2017): 189–201

Paxillin facilitates timely neurite initiation on soft-substrate environments by interacting with the endocytic machinery
Authors: Chang, Ting-Ya and Chen, Chen and Lee, Min and Chang, Ya-Chu and Lu, Chi-Huan and Lu, Shao-Tzu and Wang, De-Yao and Wang, Aijun and Guo, Chin-Lin and Cheng, Pei-Lin
Journal: eLife (2017): e31101

Study on the Regulation of Focal Adesions and Cortical Actin by Matrix Nanotopography in 3D Environment
Authors: Han, Jingjing and Lin, Keng-hui and Chew, Lock Yue
Journal: Journal of Physics: Condensed Matter (2017)

The correlation between osteopontin adsorption and cell adhesion to mixed self-assembled monolayers of varying charges and wettability
Authors: Hao, Lijing and Li, Tianjie and Yang, Fan and Zhao, Naru and Cui, Fuzhai and Shi, Xuetao and Du, Chang and Wang, Yingjun
Journal: Biomaterials Science (2017)

Cell Morphology Modified by Biomaterial Induces miR-21 Expression and Apoptosis
Authors: Liu, Mengya and Gu, Shuangying and Zhou, Yue
Journal: Journal of Biosciences and Medicines (2016): 42

说明书
鬼笔环肽-PEG4-DBCO.pdf