标签归档：缓冲液

ReadiUse TCEP 去除缓冲液 货号5540-AAT Bioquest荧光染料

发表于由

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品，欢迎访问AAT Bioquest荧光染料官网了解更多信息。

ReadiUse TCEP 去除缓冲液

ReadiUse TCEP 去除缓冲液

ReadiUse TCEP 去除缓冲液 货号5540 货号 5540 存储条件 在零下15度以下保存, 避免光照
规格 1 ml 价格 2604
Ex (nm) Em (nm)
分子量 N/A 溶剂 Water
产品详细介绍

简要概述

产品基本信息

货号：5540

产品名称：ReadiUse TCEP 去除缓冲液

规格：1ml

储存条件：-15℃避光防潮

保质期：12个月

 

产品物理化学光谱特性

分子量：N/A

溶剂：水

激发波长（nm）：N/A

发射波长（nm）：N/A

 

产品介绍

TCEP是一种常用的还原剂，广泛用于破坏蛋白质内和蛋白质之间的二硫键。 与其他两种最常见的还原剂，即二硫苏糖醇（DTT）和2-巯基乙醇相比，TCEP是无味，更强力的还原剂，不可逆还原剂，更亲水，更耐空气氧化的优点。TCEP还用于RNA分离的组织匀浆过程。 然而，有许多报道称TCEP与通常具有对TCEP反应的组分的下游测定不相容。 我们的ReadiUse TCEP去除缓冲液可有效用于消除残留的TCEP。 这是一种预先格式化的2M解决方案，可以简单地添加到包含残余TCEP的解决方案中。金畔生物是AAT Bioquest的中国代理商，为您提供最优质的FMOC-(S)-甲基烯丙基甘氨酸。

 

参考文献

A high-throughput hydrophilic interaction liquid chromatography coupled with a charged aerosol detector method to assess trisulfides in IgG1 monoclonal antibodies using tris(2-carboxyethyl)phosphine reaction products: Tris(2-carboxyethyl)phosphine-oxide and tris(2-carboxyethyl)phosphine-sulfide
Authors: Cornell C, Karanjit A, Chen Y, Jacobson F.
Journal: J Chromatogr A (2016): 107

Chemical Etching of Bovine Serum Albumin-Protected Au25 Nanoclusters for Label-Free and Separation-Free Ratiometric Fluorescent Detection of Tris(2-carboxyethyl)phosphine
Authors: Shu T, Wang J, Su L, Zhang X.
Journal: Anal Chem (2016): 11193

Palladium(II) complexes with tris(2-carboxyethyl)phosphine, structure, reactions and cytostatic activity
Authors: Pruchnik H, Lis T, Latocha M, Zielinska A, Pruchnik FP.
Journal: J Inorg Biochem (2016): 14

The Role of Tris(2-carboxyethyl)phosphine Reducing Agent in the Controlled Formation of alpha,omega-Alkanedithiols Monolayers on Au(111) with Monocoordinated and Bicoordinated Configurations
Authors: Euti EM, Velez-Romero P, Leiva EP, Macagno VA, Paredes-Olivera PA, Patrito EM, Cometto FP.
Journal: Langmuir (2016): 9428

Dissociation and reduction of covalent beta-lactoglobulin-quinone adducts by dithiothreitol, tris(2-carboxyethyl)phosphine, or sodium sulfite
Authors: Jongberg S, Lund MN, Otte J.
Journal: Anal Biochem (2015): 40

Direct measurement of active thiol metabolite levels of clopidogrel in human plasma using tris(2-carboxyethyl)phosphine as a reducing agent by LC-MS/MS
Authors: Park JB, Bae SH, Jang SM, Noh WJ, Hong JH, Yoon KD, Kang HC, Bae SK.
Journal: J Sep Sci (2013): 2306

Loss of cationic peptides with agarose gel-immobilized tris[2-carboxyethyl]phosphine (TCEP)
Authors: Shriver-Lake LC, North SH, Rowe Taitt C.
Journal: Biotechniques (2013): 292

Microwave-mediated reduction of disulfide bridges with supported (tris(2-carboxyethyl)phosphine) as resin-bound reducing agent
Authors: Miralles G, Verdie P, Puget K, Maurras A, Martinez J, Subra G.
Journal: ACS Comb Sci (2013): 169

Tris-(2-carboxyethyl) phosphine significantly promotes the reaction of cisplatin with Sp1 zinc finger protein
Authors: Chen S, Jiang H, Wei K, Liu Y.
Journal: Chem Commun (Camb) (2013): 1226

On-line cleavage of disulfide bonds by soluble and immobilized tris-(2-carboxyethyl)phosphine using sequential injection analysis
Authors: Tzanavaras PD, Mitani C, Anthemidis A, Themelis DG.
Journal: Talanta (2012): 21

说明书
ReadiUse TCEP 去除缓冲液.pdf

Screen Quest 10X细胞染色缓冲液 *含酚红* 货号36300-AAT Bioquest荧光染料

发表于由

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品，欢迎访问AAT Bioquest荧光染料官网了解更多信息。

Screen Quest 10X细胞染色缓冲液 *含酚红*

Screen Quest 10X细胞染色缓冲液 *含酚红*

Screen Quest 10X细胞染色缓冲液 *含酚红* 货号36300 货号 36300 存储条件 在零下15度以下保存, 避免光照
规格 10 mL 价格 1272
Ex (nm) Em (nm)
分子量 溶剂
产品详细介绍

简要概述

产品基本信息

货号：36300

产品名称：Screen Quest 10X细胞染色缓冲液

规格：10ml

储存条件：-15℃避光防潮

保质期：24个月

 

产品介绍

即用型缓冲液，针对荧光细胞成像进行了优化。 在某些情况下，此缓冲液可明显增强成像信号。金畔生物是AAT Bioquest的中国代理商，为您提供最优质的Screen Quest 10X细胞染色缓冲液。 

 

图示

Screen Quest 10X细胞染色缓冲液 *含酚红* 货号36300

图1.带有Phenol Red Plus 的Screen Quest 10X细胞染色缓冲液的图
Screen Quest 10X细胞染色缓冲液 *含酚红* 货号36300

图2.使用Cal-520 AM在CHO-M1细胞中测量了ATP剂量反应。 将CHO-M1细胞以50,000个细胞/ 100 µL /孔在黑色96孔板中过夜接种。 加入含1X Phenol Red Plus 细胞染色缓冲液的HH缓冲液中的100 µL 10ug / ml Cal-520 AM，并在37oC下孵育60分钟。 加入ATP（50µL /孔）以达到最终指示的浓度。

 

参考文献

Kinetic characterization of novel NR2B antagonists using fluorescence detection of calcium flux
Authors: Bednar B, Cunningham ME, Kiss L, Cheng G, McCauley JA, Liverton NJ, Koblan KS.
Journal: J Neurosci Methods (2004): 247

Novel fluo-4 analogs for fluorescent calcium measurements
Authors: Martin VV, Beierlein M, Morgan JL, Rothe A, Gee KR.
Journal: Cell Calcium (2004): 509

Amplitude distribution of calcium sparks in confocal images: theory and studies with an automatic detection method
Authors: Cheng H, Song LS, Shirokova N, Gonzalez A, Lakatta EG, Rios E, Stern MD.
Journal: Biophys J (1999): 606

Flow cytometric kinetic assay of calcium mobilization in whole blood platelets using Fluo-3 and CD41
Authors: do Ceu Monteiro M, Sansonetty F, Goncalves MJ, O’Connor JE.
Journal: Cytometry (1999): 302

A simple numerical model of calcium spark formation and detection in cardiac myocytes
Authors: Smith GD, Keizer JE, Stern MD, Lederer WJ, Cheng H.
Journal: Biophys J (1998): 15

Monitoring calcium in outer hair cells with confocal microscopy and fluorescence ratios of fluo-3 and fura-red
Authors: Su ZL, Li N, Sun YR, Yang J, Wang IM, Jiang SC.
Journal: Shi Yan Sheng Wu Xue Bao (1998): 323

Loading and localization of Fluo-3 and Fluo-3/AM calcium indicators in sinapis alba root tissue
Authors: Tretyn A, Kado RT, Kendrick RE.
Journal: Folia Histochem Cytobiol (1997): 41

Nucleoplasmic and cytoplasmic differences in the fluorescence properties of the calcium indicator Fluo-3
Authors: Perez-Terzic C, Stehno-Bittel L, Clapham DE.
Journal: Cell Calcium (1997): 275

Detection of a trigger zone of bradykinin-induced fast calcium waves in PC12 neurites
Authors: Reber BF, Schindelholz B.
Journal: Pflugers Arch (1996): 893

Improved four-color flow cytometry method using fluo-3 and triple immunofluorescence for analysis of intracellular calcium ion ([Ca2+]i) fluxes among mouse lymph node B- and T-lymphocyte subsets
Authors: Greimers R, Trebak M, Moutschen M, Jacobs N, Boniver J.
Journal: Cytometry (1996): 205

说明书
Screen Quest 10X细胞染色缓冲液 *含酚红*.pdf

荧光素-12-dGTP *1 mM Tris 缓冲液 (pH 7.5)* 货号17037-AAT Bioquest荧光染料

发表于由

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品，欢迎访问AAT Bioquest荧光染料官网了解更多信息。

荧光素-12-dGTP *1 mM Tris 缓冲液 (pH 7.5)*

荧光素-12-dGTP *1 mM Tris 缓冲液 (pH 7.5)*

荧光素-12-dGTP *1 mM Tris 缓冲液 (pH 7.5)* 货号17037 货号 17037 存储条件 在零下15度以下保存, 避免光照
规格 25 nmoles 价格 1164
Ex (nm) 498 Em (nm) 517
分子量 1030.72 溶剂 Water
产品详细介绍

简要概述

产品基本信息

货号：17037

产品名称：荧光素-12-dGTP *1 mM Tris 缓冲液 (pH 7.5)*

规格：25 nmoles

储存条件：-15℃避光防潮

保质期：6个月

 

产品物理化学光谱特性

分子量：1030.72

Ex(nm):498

Em(nm):517

外观：液体

溶剂：水

 

产品介绍

荧光素-12-dGTP 可以作为其天然对应物 dGTP 掺入 DNA 序列中，从而将荧光素标签掺入 DNA 分子中。它可用于 DNA/cDNA 的直接酶促标记，例如通过切口平移、随机引物、聚合酶链反应 (PCR) 和 3′-末端标记。所得荧光素标记的 DNA/cDNA 探针非常适合荧光杂交应用，例如 FISH 或基于微阵列的基因表达谱分析。荧光素标签和鸟苷之间的接头已针对最佳底物掺入和标记效率进行了优化。标记的产物可以通过荧光直接检测或通过与抗荧光素 HRP 或 AP 偶联物结合间接检测。

点击查看光谱

说明书
荧光素-12-dGTP *1 mM Tris 缓冲液 (pH 7.5)*.pdf

iFluor 647-PEG12-dUTP *1 mM Tris 缓冲液 (pH 7.5)* 货号17043-AAT Bioquest荧光染料

发表于由

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品，欢迎访问AAT Bioquest荧光染料官网了解更多信息。

iFluor 647-PEG12-dUTP *1 mM Tris 缓冲液 (pH 7.5)*

iFluor 647-PEG12-dUTP *1 mM Tris 缓冲液 (pH 7.5)*

iFluor 647-PEG12-dUTP *1 mM Tris 缓冲液 (pH 7.5)* 货号17043 货号 17043 存储条件 在零下15度以下保存, 避免光照
规格 25 nmoles 价格 1164
Ex (nm) 656 Em (nm) 670
分子量 2293.72 溶剂 Water
产品详细介绍

简要概述

产品基本信息

货号：17043

产品名称：iFluor 647-PEG12-dUTP *1 mM Tris 缓冲液 (pH 7.5)*

规格：25 nmoles

储存条件：-15℃避光防潮

保质期：12个月

 

产品物理化学光谱特性

分子量：2293.72

溶剂：水

外观：液体

激发波长(nm)：656

发射波长(nm)：670

 

产品介绍

染料修饰的脱氧尿苷 5′-三磷酸是通过常规酶促掺入方法（如逆转录、切口平移、随机引物标记或 PCR）生产染料标记 DNA 的最常用方法之一。 这种酶促荧光标记方法广泛用于 FISH 探针和基于微阵列的实验。 这种 iFluor 647-PEG12-dUTP 缀合物具有与 ChromaTide Alexa Fluor 647-dUTP 相同的光谱特性。 其 PEG12 间隔区使其更有效地通过聚合酶整合到 DNA 中。 它用作带有 SpectrumFRed 滤光片组的远红色荧光颜色。 它提供了具有高光稳定性且不受 pH 影响的明亮信号。

点击查看光谱

 

参考文献

Loss of PDPK1 abrogates resistance to gemcitabine in label-retaining pancreatic cancer cells.
Authors: Li, Dandan and Mullinax, John E and Aiken, Taylor and Xin, Hongwu and Wiegand, Gordon and Anderson, Andrew and Thorgeirsson, Snorri and Avital, Itzhak and Rudloff, Udo
Journal: BMC cancer (2018): 772

dUTPs conjugated with zwitterionic Cy3 or Cy5 fluorophore analogues are effective substrates for DNA amplification and labelling by Taq polymerase.
Authors: Zasedateleva, Olga A and Vasiliskov, Vadim A and Surzhikov, Sergey A and Kuznetsova, Viktoriya E and Shershov, Valeriy E and Guseinov, Timur O and Smirnov, Igor P and Yurasov, Roman A and Spitsyn, Maksim A and Chudinov, Alexander V
Journal: Nucleic acids research (2018): e73

Mass-spectrometry analysis of modifications at DNA termini induced by DNA polymerases.
Authors: Smirnov, Igor P and Kolganova, Natalia A and Vasiliskov, Vadim A and Chudinov, Alexander V and Timofeev, Edward N
Journal: Scientific reports (2017): 6674

Separation of replication and transcription domains in nucleoli.
Authors: Smirnov, E and Borkovec, J and Kováčik, L and Svidenská, S and Schröfel, A and Skalníková, M and Švindrych, Z and Křížek, P and Ovesný, M and Hagen, G M and Juda, P and Michalová, K and Cardoso, M C and Cmarko, D and Raška, I
Journal: Journal of structural biology (2014): 259-66

A prenatally ascertained, maternally inherited 14.8 Mb duplication of chromosomal bands Xq13.2-q21.31 associated with multiple congenital abnormalities in a male fetus.
Authors: Sismani, C and Donoghue, J and Alexandrou, A and Karkaletsi, M and Christopoulou, S and Konstantinidou, A E and Livanos, P and Patsalis, P C and Velissariou, V
Journal: Gene (2013): 138-42

[Establishment of a hydrogel chip for high-throughput detection of Y chromosome microdeletions].
Authors: Li, You-Zhi and Chen, Zhi-Yao and Wang, Hui and Huang, Huan and Song, Qin-Xin and Zhou, Guo-Hua
Journal: Zhonghua nan ke xue = National journal of andrology (2012): 109-14

Selection of reliable reference genes for gene expression study in nasopharyngeal carcinoma.
Authors: Guo, Yi and Chen, Jia-xin and Yang, Shu and Fu, Xu-ping and Zhang, Zheng and Chen, Ke-he and Huang, Yan and Li, Yao and Xie, Yi and Mao, Yu-min
Journal: Acta pharmacologica Sinica (2010): 1487-94

Time-resolved three-dimensional molecular tracking in live cells.
Authors: Wells, Nathan P and Lessard, Guillaume A and Goodwin, Peter M and Phipps, Mary E and Cutler, Patrick J and Lidke, Diane S and Wilson, Bridget S and Werner, James H
Journal: Nano letters (2010): 4732-7

[Expression of COX10 in human non-obstructive azoospermia testes].
Authors: Yang, Bo and Yuan, Jian-Lin and Gao, Xiao-Kang and Wang, He and Shao, Chen and Liu, He-Liang and Chen, Bao-Qi and Qin, Rong-Liang and Shao, Guo-Xing and Kang, Fu-Xia
Journal: Zhonghua nan ke xue = National journal of andrology (2009): 599-603

[Expression of cell cycle molecules in human azoospermic testes].
Authors: Yang, Bo and Yuan, Jian-lin and Gao, Xiao-kang and Qin, Wei-jun and Liu, Fei and Shao, Chen and Liu, He-liang and Kang, Fu-xia
Journal: Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology (2009): 393-5

说明书
iFluor 647-PEG12-dUTP *1 mM Tris 缓冲液 (pH 7.5)*.pdf

Tris缓冲液（pH 7.5）中的生物素-14-dCTP * 1 mM * 货号17019-AAT Bioquest荧光染料

发表于由

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品，欢迎访问AAT Bioquest荧光染料官网了解更多信息。

Tris缓冲液（pH 7.5）中的生物素-14-dCTP * 1 mM *

Tris缓冲液（pH 7.5）中的生物素-14-dCTP * 1 mM *

Tris缓冲液（pH 7.5）中的生物素-14-dCTP * 1 mM * 货号17019 货号 17019 存储条件 在零下15度以下保存, 避免光照
规格 25 nmoles 价格 1272
Ex (nm) Em (nm)
分子量 905.79 溶剂 Water
产品详细介绍

简要概述

产品基本信息

货号：17019

产品名称：Tris缓冲液（pH 7.5）中的生物素-14-dCTP * 1 mM *

规格：25 nmoles

储存条件：-15℃避光防潮

保质期：12个月

 

产品物理化学光谱特性

分子量：905.79

溶剂：水

激发波长(nm)：N/A

发射波长(nm)：N/A

 

产品介绍

Tris缓冲液（pH 7.5）中的生物素-14-dCTP * 1 mM *是美国AAT Bioquest生产的荧光染料，生物素修饰的dCTP类似物广泛用于各种非放射性DNA标记反应，包括切口平移，随机引物标记，cDNA标记和3′-末端标记。已经显示生物素化的探针以与非生物素化的探针相同的速率和相同的程度与同源核酸杂交。亲和素和链霉亲和素可以检测到杂交的生物素化DNA探针。将生物素-14-dCTP酶促地掺入DNA / cDNA中，以替代dCTP。随后使用与辣根过氧化物酶（HRP），碱性磷酸酶（AP），荧光染料或琼脂糖/磁珠偶联的抗生蛋白链菌素检测所得的生物素标记的DNA / cDNA探针。在胞嘧啶N4位置和生物素标签之间的14原子间隔子将CTP碱基与生物素标签分开，从而确保了尼克翻译的最佳性能。对于使用Taq聚合酶进行PCR掺入实验，可以将50％Biotin-14-dCTP / 50％dCTP比率用于Nick翻译。或者，您可以在测试条件下确定最佳比率。金畔生物是AAT Bioquest的中国代理商，为您提供最优质的Tris缓冲液（pH 7.5）中的生物素-14-dCTP * 1 mM *。

 

参考文献

A visual DNA chip for simultaneous detection of hepatitis B virus, hepatitis C virus and human immunodeficiency virus type-1
Authors: Wen JK, Zhang XE, Cheng Z, Liu H, Zhou YF, Zhang ZP, Yang JH, Deng JY.
Journal: Biosens Bioelectron (2004): 685

Fluorescent DNA hybridization probe preparation using amine modification and reactive dye coupling
Authors: Cox WG, Singer VL.
Journal: Biotechniques (2004): 114

Aminomodified nucleobases: functionalized nucleoside triphosphates applicable for SELEX
Authors: Schoetzau T, Langner J, Moyroud E, Roehl I, Vonhoff S, Klussmann S.
Journal: Bioconjug Chem (2003): 919

Simple method for preparation of fluor/hapten-labeled dUTP
Authors: Nimmakayalu M, Henegariu O, Ward DC, Bray-Ward P.
Journal: Biotechniques (2000): 518

Topology of yeast RNA polymerase II subunits in transcription elongation complexes studied by photoaffinity cross-linking
Authors: Wooddell CI, Burgess RR.
Journal: Biochemistry (2000): 13405

Quantitative analysis of polymerase chain reaction products using biotinylated dUTP incorporation
Authors: Duplaa C, Couffinhal T, Labat L, Moreau C, Lamaziere JM, Bonnet J.
Journal: Anal Biochem (1993): 229

A non-radioisotopic reverse transcriptase assay using biotin-11-deoxyuridinetriphosphate on primer-immobilized microtiter plates
Authors: Urabe T, Sano K, Tanno M, Mizoguchi J, Otani M, Lee MH, Takasaki T, Kusakabe H, Imagawa DT, Nakai M.
Journal: J Virol Methods (1992): 145

Affinity isolation of active murine erythroleukemia cell chromatin: uniform distribution of ubiquitinated histone H2A between active and inactive fractions
Authors: Dawson BA, Herman T, Haas AL, Lough J.
Journal: J Cell Biochem (1991): 166

Non-radioactive labeling and detection of nucleic acids. IV. Synthesis and properties of digoxigenin-modified 2′-deoxyuridine-5′-triphosphates and a photoactivatable analog of digoxigenin (photodigoxigenin)
Authors: Muhlegger K, Huber E, von der Eltz H, Ruger R, Kessler C.
Journal: Biol Chem Hoppe Seyler (1990): 953

 

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说明书
Tris缓冲液（pH 7.5）中的生物素-14-dCTP * 1 mM *.pdf